A disclosed urinary microalbumin detection apparatus comprises a box holder, a box cover and a reagent strip; the box cover is provided with a sample adding hole and an observation port; the reagent strip comprises a support base sheet and a chromatography structure disposed on the base sheet; the chromatography structure comprises sample filtering paper, an immune colloidal gold paper sheet, an immune nitrocellulose membrane and water adsorption paper which are in successive head-to-tail connection arrangement; and the apparatus is characterized in that the sample adding hole also is provided with an urine cup opened at the bottom, and the bottom opening of the urine cup has the shape and the size both same to those of the sample adding hole, the immune nitrocellulose membrane is provided with a quality control band and multiple detection lines, and the quality control band and the multiple detection lines are exposed in the observation port. The urinary microalbumin detection apparatus has the advantages of convenient operation, rapid reaction, high sensitivity, high specificity, economy and utility, uneasiness to contaminate hands, capability of quantificationally obtaining the concentration interval of urinary microalbumin, and the like, and is suitable for on-site detection and personal usage.