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Urine microalbumin detection method and kit

A urine microalbumin and solution technology, which is applied in the field of urine microalbumin detection methods, kits and systems, can solve the problems of affecting the health of operators, polluting the environment, and difficulty in popularization, and achieves low cost of testing, simple operation, Guarantee the effect of the application test effect

Inactive Publication Date: 2013-05-08
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, radioimmunity uses radioactive isotopes as tracers, which affects the health of operators and seriously pollutes the environment
Other detection methods also have the characteristics of being difficult to popularize due to the limitations of instrument and reagent conditions.

Method used

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  • Urine microalbumin detection method and kit
  • Urine microalbumin detection method and kit
  • Urine microalbumin detection method and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] This example verifies the urine microalbumin concentration of three urine samples. The actual urine microalbumin concentration of each urine sample is as follows: urine sample 1 is 5.84 mg / L, urine sample 2 is 22.25 mg / L, urine Sample 3 is 68.17mg / L. The cyanine dye used in this embodiment is a compound of the following formula

[0051]

[0052] 1) Prepare standard solution and standard colorimetric solution

[0053] A certain amount of urine microalbumin was dissolved in Tris-HCl (Tris-Na) buffer (pH 8.0, buffer concentration 2 mmol / L) containing 20 mmol / L NaCl, and the preparation concentrations were 0, 10, 20, The urine microalbumin standard solution of 50, 100, 150, 200 mg / L is reserved.

[0054] Take 200μL of the cyanine dye methanol solution with a concentration of 200μmol / L, and add 9.8ml of Tris-HCl (Tris-Na) buffer (pH8.0, buffer concentration 2mmol / L) containing 20mmol / L NaCl to obtain a cyanine solution containing 4μmol / L Dye solution. Divide the above-mentioned...

Embodiment 2

[0061] This example verifies the urine microalbumin concentration of three urine samples. The actual urine microalbumin concentration of each urine sample is as follows: urine sample 1 is 9.18 mg / L, urine sample 2 is 32.43 mg / L, urine Sample 3 is 149.57mg / L. The cyanine dye used in this embodiment is a compound of the following formula

[0062]

[0063] 1) Prepare standard solution and standard colorimetric solution

[0064] A certain amount of urine microalbumin was dissolved in Tris-HCl (Tris-K) buffer (pH 7.0, buffer concentration 30 mmol / L) containing 50 mmol / L KCl, and the preparation concentrations were 0, 10, 20, The urine microalbumin standard solution of 50, 100, 150, 200 mg / L is reserved.

[0065] Take 300μL of methanol solution of cyanine dye with a concentration of 200μmol / L, and add 9.7ml Tris-HCl (Tris-K) buffer (pH7.0, buffer concentration 30mmol / L) containing 50mmol / L KCl to obtain 6μmol / L cyanine Dye solution. Divide the above-mentioned cyanine dye solution into ...

Embodiment 3

[0072] This example verifies the urine microalbumin concentration of three urine samples. The actual urine microalbumin concentration of each urine sample is as follows: urine sample 1 is 9.82 mg / L, urine sample 2 is 20.35 mg / L, urine Sample 3 is 78.26mg / L. The cyanine dye used in this embodiment is a compound of the following formula

[0073]

[0074] 1) Prepare standard solution and standard colorimetric solution

[0075] Dissolve a certain amount of urinary microalbumin in sodium phosphate buffer (pH 6.0, buffer concentration 5mmol / L) to prepare urinary traces with concentrations of 0, 5, 10, 15, 20, 50, 100 mg / L Albumin standard solution, set aside.

[0076] Take 600 μL of the methanol solution of the cyanine dye with a concentration of 200 μmol / L and add 4.4 ml of sodium phosphate buffer (pH 6.0, buffer concentration 5 mmol / L) to obtain a solution containing 24 μmol / L of the cyanine dye. Divide the above-mentioned cyanine dye solution into 10 equal parts, each of which is 0.5...

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Abstract

The invention relates to a urine microalbumin detection method and a kit. The urine microalbumin detection method comprises the following steps of: (1) preparing a plurality of standard solutions witg different urine microalbumin concentrations from a metal-ion-contained buffer solution and microalbumin, wherein the pH of the buffer solution is 5.0-8.2; (2) adding a same amount of cyanine dyes and the metal-ion-contained buffer solution in the plurality of standard solutions to obtain a plurality of standard colorimetric solutions; (3) adding the cyanine dyes and the metal-ion-contained buffer solution in a to-be-tested liquid sample, so as to enable the concentrations and the pH values of the cyanine dyes in the to-be-tested sample to be same as those of the standard colorimetric solutions, so as to obtain a test solution; and (4) comparing the color of the test solution with that of the standard colorimetric solutions obtained in the step (2), finding out one standard colorimetric solution of which the color is the same or close to that of the test solution from the plurality of standard colorimetric solutions, so as to obtain the result that the urine microalbumin concentration of the standard solution corresponding to the standard colorimetric solution is the same or close to that of the to-be-tested liquid.

Description

Technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to a urine microalbumin detection method, kit and system. Background technique [0002] Microalbuminuria refers to the appearance of microalbumin in the urine. Albumin is a normal protein in the blood, but only a very small amount of albumin appears in the urine under physiological conditions. Microalbuminuria reflects abnormal protein leakage from the kidneys. The increase in urine microalbumin is more common in diabetic nephropathy, hypertension, preeclampsia, and is an early sensitive indicator of kidney injury. Urine microprotein indicators are usually used to monitor the occurrence of kidney disease. The detection of urinary microprotein is the most sensitive and reliable diagnostic indicator for early detection of kidney disease. [0003] Urine microalbumin is to assess the degree of kidney damage: when it is found that the urine microalbumin is in the range of 20mg / L-200mg / ...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/80
CPCC09B23/0008C09B23/06
Inventor 孙红霞唐亚林杨千帆管爱娇
Owner INST OF CHEM CHINESE ACAD OF SCI
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