The invention provides a method for simultaneously detecting the content of 16 novel brominated
flame retardants in human serum. The method comprises the following specific steps: S1, preparing a mixed standard working solution; s2, preparing an
internal standard working solution; s3, drawing a standard working curve; s4, sample pretreatment: adding
dichloromethane into a glass
test tube, adding an
internal standard working solution, carrying out water bath at 40 DEG C, carrying out
nitrogen blowing until the solution is nearly dry, adding fetal calf serum and pure water, and carrying out vortex uniform mixing; the method comprises the following steps: absorbing a
mixed solution of
dichloromethane and
acetonitrile and a sample to form an
emulsion, carrying out vortex full extraction on the
emulsion by using a dispersing agent and an extracting agent, and centrifuging at
room temperature; finally, sucking an organic
precipitation phase, filtering through a filter membrane, transferring into a
small sample introduction
bottle with a glass lining tube, and standing for on-
machine detection; and S5, detection and result analysis: carrying out detection and result analysis on the content of the novel
brominated flame retardant by adopting a
gas chromatography-tandem
triple quadrupole mass spectrometer. The method disclosed by the invention is simple in pretreatment, high in sensitivity and strong in practicability, and can be used for conventional biological monitoring of the NBFRs of the crowd.