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Method for determining water quality biotoxicity

A biological toxicity and water quality technology, applied in the field of environmental monitoring, water quality online monitoring of biological fish, can solve the problems of insensitivity, consumption of test materials, inaccurate results, etc., and achieve the effect of improving timeliness and ensuring the safety of water supply and water quality

Inactive Publication Date: 2015-08-19
SHANDONG JIANZHU UNIV +1
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Problems solved by technology

At present, most detection methods use biological monitoring equipment for water quality biological toxicity, but only for comprehensive index evaluation, which is the result of the overall effect of pollutants on aquatic organisms, which consumes a lot of test materials, and it is easy to ignore its impact on organisms. Some aspects of the body itself, resulting in inaccurate and insensitive results, while wasting a lot of manpower and material resources

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  • Method for determining water quality biotoxicity
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Embodiment 1

[0027] A method for measuring water quality biological toxicity, comprising the steps of:

[0028] (1) Put the zebrafish in standard dilution water with room temperature of 25°C and continuous aeration, feed and remove feces and food residues every day. The domestication time is 7 days, and the feeding is stopped the day before the test; the mortality of the zebrafish must be guaranteed during the domestication period Less than 10%, if the mortality rate of zebrafish is higher than 10%, then replace a batch of fish domestication;

[0029] (2) Place domesticated zebrafish in standard dilution water and different concentrations of Cr prepared in standard dilution water 6+ In solution, Cr 6+ The concentration of the solution is 25mg / L, 40mg / L, 50mg / L, 75mg / L and 100 mg / L, a total of 6 experiments, each group is set up 3 parallel groups, the number of zebrafish in each group is 5, and Cr 6+ In the acute toxicity test under stress, the mortality rate of zebrafish within 48 hours ...

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Abstract

The invention discloses a method for determining water quality biotoxicity, which comprises the following steps: domesticating zebra fish; performing acute toxicity test of the domesticated zebra fish and calculating medial lethal concentration of 48 hours; performing behavioristics test of the domesticated zebra fish, determining movement speed change of the zebra fish and judging water quality biotoxicity. The movement speed of the zebra fish in a water environment is used as a biomarker to evaluate the biotoxicity of a pollutant, the movement behavior of the zebra fish in the water environment is traced and captured, the movement trail of the zebra fish is intuitively observed anytime, a speed index is determined in real time, the quantified evaluation of the water quality biotoxicity is determined by using the movement speed change of the zebra fish, a water quality fluctuation condition is reflected in short time, timeliness, sensitivity and accuracy of biological monitoring early warning are improved, an early warning technical support is provided for acute water body pollution, and safety of water quality of supplied water is guaranteed.

Description

technical field [0001] The invention relates to the field of online water quality monitoring biological fish, in particular to a method for measuring water quality biological toxicity, which belongs to the field of environmental monitoring. Background technique [0002] Human beings cannot live without water, and the quality of drinking water is closely related to human health. With the development of society and economy, the progress of science and the improvement of people's living standards, people's requirements for the quality of drinking water have been continuously improved, and the standards for drinking water quality have also been continuously developed and improved accordingly. The impact of drinking water on human health is not only reflected in the content of a certain substance, but in the harm of all substances contained in drinking water to the human body. Therefore, the comprehensive toxicity of drinking water is an important way to control the quality and s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/18
CPCY02A20/20
Inventor 李梅贾瑞宝李汝宋武昌逯南南张克峰
Owner SHANDONG JIANZHU UNIV
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