Seashell monitoring method for fresh water environment heavy metal pollution and microelement accumulation

A trace element and pollution monitoring technology, which is applied in the direction of general water supply saving, testing water, measuring devices, etc., to achieve the effect of simple pretreatment method, strong comparability and good stability

Inactive Publication Date: 2008-02-13
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, except for some exploratory studies in our laboratory, there are no reports at home and abroad on the use of shellfish to monitor persistent pollutant pollution in freshwater environments; therefore, the selection of suitable biological indicator mussel species, the establishment of monitoring methods and monitoring results The reliability check of is the key point of the present invention

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A shellfish monitoring method for heavy metal pollution and trace element accumulation in a freshwater environment of the present invention adopts the following process steps:

[0023] The samples of Anodonta dorsalis (40 individuals) were collected from four sampling points around Taihu Lake in 2006: Wuli Lake in Wuxi, Huzhou, Manshan in Suzhou and Dapu in Yixing.

[0024] The collected live dentate mussels were temporarily raised in aerated water for 3 days to empty the intestinal debris, and then the biological properties such as shell length, shell height, shell width, and shell weight were measured. Then the soft tissue was dissected to obtain the soft tissue, which was stored in a -20°C refrigerator after weighing. After thawing, rinse with ultrapure water (Millipro, USA, Milliprore-Simplicity personal ultrapure water system, outlet water resistivity 18.2MΩ.cm) water 6 times, dry in a drying oven at 70°C for 18h to constant weight; The dry samples of the soft tis...

Embodiment 2

[0028] A shellfish monitoring method for heavy metal pollution and trace element accumulation in a freshwater environment of the present invention adopts the following process steps:

[0029] The samples of Anodonta dorsalis (40 individuals) were collected from four sampling points around Taihu Lake in 2006: Wuli Lake in Wuxi, Huzhou, Manshan in Suzhou and Dapu in Yixing.

[0030] The collected live dentate mussels were temporarily raised in aerated water for 4 days to empty the intestinal debris, and then the biological properties such as shell length, shell height, shell width, and shell weight were measured. Then the soft tissue was dissected to obtain the soft tissue, which was stored in a -18°C refrigerator after weighing. After thawing, rinse with ultrapure water (Millipro, USA, Milliprore-Simplicity personal ultrapure water system, outlet water resistivity 18.2MΩ.cm) water 7 times, dry in a drying oven at 80°C for 12h to constant weight; The dry samples of the soft tis...

Embodiment 3

[0034] A shellfish monitoring method for heavy metal pollution and trace element accumulation in a freshwater environment of the present invention adopts the following process steps:

[0035] The samples of Anodonta dorsalis (40 individuals) were collected from four sampling points around Taihu Lake in 2006: Wuli Lake in Wuxi, Huzhou, Manshan in Suzhou and Dapu in Yixing.

[0036] The collected live dentate mussels were kept temporarily in aerated water for a period of time to make them empty the intestinal debris, and then measure the biological properties such as shell length, shell height, shell width, and shell weight. Then the soft tissues were dissected and weighed and stored in a -19°C refrigerator for use. After thawing, rinse with ultrapure water (Millipro, USA, Milliprore-Simplicity personal ultrapure water system, outlet water resistivity 18.2MΩ.cm) water 8 times, dry in a drying oven at 60°C for 20h to constant weight; The dry samples of the soft tissue of the hor...

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Abstract

The invention relates to a shellfish monitoring method of freshwater environment heavy metal pollution and trace elements accumulation, which belongs to the biological monitoring environment technology field. The invention is characterized in that anodonta woodiana living heudes are selected to be raised in aerated water. Impurities in a shell and a digestive tract are eliminated. Then, the heudes are dissected and soft tissues of the heudes are obtained and preserved in a refrigerator. A heude soft tissue dry sample is fetched and put in a vessel washed with acid. Acid hydrolysis happens at room temperature. A microwave oven is used for low fire resolution. Water is used to determine volume and solution to be detected is prepared. The invention has the advantages of convenient sampling and simple pretreatment method. With an inductively coupled plasma mass spectrometer, trace elements pollutants in shellfish anodonta woodiana living heudes can be precisely determined and indicated. Concentrations, components characteristics, pollution degree, biological toxicity effects and differences between waters of various pollutants can be rapidly known. The invention has the advantages of good stability, high reproducibility, high comparability and small system error. Harm risks on fishery waters and development trends of all pollutants can be precisely assessed and predicated.

Description

technical field [0001] The invention relates to a shellfish monitoring method for heavy metal pollution and trace element accumulation in a freshwater environment, which can systematically and rapidly monitor various heavy metal pollution and trace element accumulation levels, water features, etc. in the water environment, and belongs to the technical field of biological monitoring environment . Background technique [0002] In recent years, the pollution of heavy metals and other trace elements in the water environment has restricted the rapid development of my country's economy, and the tendency to threaten people's health and quality of life has become increasingly obvious, and some have even caused major disasters. Inland fishery is a very important part of my country's fishery industry. The ecological environment of fishery waters is the foundation of fishery development. Changes in the quantity of inland fishery resources and the healthy development of aquaculture ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/18G01N27/62
CPCY02A20/20
Inventor 杨健刘洪波
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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