38 results about "Bivalve mollusk" patented technology

The invention relates to a bottom sowing-type mariculture facility which is suitable for the sea area with silt bottom, in particular to a bottom sowing-type raft frame and a net cage for marine bivalve mollusks, sea cucumbers or mixotrophic culture of marine bivalve mollusks and sea cucumbers. The facility is provided with a PVC pipe, a polyethylene rope for fixing the PVC pipe, a webbing, a stake or an anchor for fixing the facilities, a rope fixed on the stake or the anchor, and a fixable floating ball which is connected to the rope and floats on the surface of water; the PVC pipe is fixed on the polyethylene rope in parallel, the polyethylene rope is arranged in parallel, is perpendicular to the PVC pipe, is positioned at the upper side and the lower side of the two terminals of the PVC pipe and forms the structure similar to a 'soft ladder'; the 'soft ladder' is externally covered by the webbing, and a plurality of independent net cages are formed by combining the PVC pipe, the polyethylene rope and the webbing; the two ends of the undrawn 'soft ladder' are fixed at the bottom of the sea by the stake or the anchor respectively, and the floating ball is fixed at the two ends of the 'soft ladder' by the rope. The facility can effectively prevent the enemies and reduce the influence of storm and ocean current, thus being convenient for management and harvest and favorable to the comprehensive utilization of mariculture area.

PCT No. PCT/IE96/00030 Sec. 371 Date Nov. 19, 1997 Sec. 102(e) Date Nov. 19, 1997 PCT Filed May 14, 1996 PCT Pub. No. WO96/36236 PCT Pub. Date Nov. 21, 1996A method of processing bivalve molluscs comprises filling a basket-like container (11) with the bivalve molluscs and vibrating the bivalve molluscs within the container (11), thereby inducing them to close tightly under stress and compacting them together. A lid (12) is closed to maintain the compact configuration of the bivalve molluscs, which are cooked by immersing the container into boiling seawater, before being removed and plunged into chilled water to halt the cooking process. The bivalve molluscs are then blast frozen. Because the shells of the bivalve molluscs are tightly closed and better compaction is achieved by vibrating the bivalve molluscs than by compressing them into the container (11), the shells are unable to open and the internal juices are retained within the shells throughout the cooking, chilling and freezing steps, thereby resulting in processed bivalve molluscs having greatly improved organoleptic qualities upon thawing.

The invention relates to a device used for matching with the measurement for respiration and excretion of aquatic animals, in particular to an attachment used for matching with various dissolved oxygen probes to measure the respiration and the excretion of various aquatic animals such as fish, shrimps as well as crabs, bivalve mollusks, sea cucumbers, etc. living in freshwater or seawater. The device takes an organic glass slab-bottom at the bottom of a crude organic glass tube as a back cover to form a vessel; a concentric-circle organic glass flange provided with holes is sleeved on the upper end of the crude organic glass tube at the height parallel to a nozzle; an organic glass cover plate is arranged on the top of the organic glass flange to be used as an upper cover; a fine organic glass tube and a PVE tube cuff are arranged on the organic glass cover plate; the PVE tube cuff is inserted into the dissolved oxygen probes in a sealing way; a diving pump is arranged under the PVE tube cuff. The device solves the problems that the prior art cannot reflect the metabolic status of the aquatic animals factually and accurately, etc., and has the advantages of simple structure, low manufacturing cost, being easy to match with correlated instruments to detect accurately, etc.

The present invention relates to a surfactant moryoupe and the preparation method and the application of the surfactant, which pertains to the technical field of pharmaceutical industry. The composition is made by extraction, purification and other steps of the tissues of bivalves mollusks, the composition contains rich phospholipid complex of the seafood which is composed of polyunsaturated fatty acids, wherein, the composition includes plasmalogen, active short-chain peptides and amino acids etc. The composition of the present invention has the surface activity of the surfactant, which can be used for preparing the drugs for replacement, therapy during the lack of pulmonary surfactants; furthermore, the invention has no immunogenicity.

The invention discloses an experiment method and system for researches on seawater environmental stresses. According to the experiment method, a mother solution of the seawater environmental stresses is added to a culture water tank with a drop method and is uniformly mixed with surrounding seawater through a mixing device, and the shellfish culture environment is changed gradually. The system comprises an environmental stress device for accommodating the mother solution of the environmental stresses and a culture device located below the environmental stress device, wherein the environmental stress device comprises an airtight water storage tank provided with a water inlet sealing screw cap above, the lower side of the airtight water storage tank is connected with a gas inlet pipe and a liquid outlet hose respectively, and a speed-changing knob is further arranged on the water outlet hose; the culture device is a culture water tank accommodating seawater, and an oxygen inflating device is further arranged at the bottom of the culture water tank; the mother solution is mixed to the culture water tank through the liquid outlet hose in a suspended dripping manner. With the adoption of the experiment method and the experiment device, the living environment of bivalve mollusks can be truly gradually changed, other interference factors are avoided, and the accuracy and the precision of the experimental researches on the seawater environmental stresses are improved.

The invention relates to the technical field of purifying heavy metal in edible marine products, and discloses a method for purifying cadmium in bivalve mollusc, which comprises the following steps: adding nano calcium carbonate suspension, magnesium ion water solution, vitamin C and casein phosphopeptide into the water body cultivating the bivalve mollusc; replacing the water body every day, reading nano calcium carbonate suspension, magnesium ion water solution, vitamin C and casein phosphopeptide. According to the invention, the method is simple in operation, the purification cost is low and the operation time is short, the Cadmium ion residue in the edible bivalve bodies can be efficiently purified, and the product quality and flavor are not affected. According to the invention, when the bivalve mollusc polluted by Cadmium is treated by using the method, the removal rate of cadmium in the bivalve mollusk bodies can reach 36.5-38.4% after 7 days.

An optimization feeding method for shrimp culture includes feeding according to the current feeding time and mode, its characteristcs are during the maturation period of shrimp to adopt natural animal baits and compound feeds by 2-4 :1 frequency or weight ratio to feed in turn; the said natural animal baits are sleeve-fishs, clamworms, minitype shrimps and crabs or bivalve mollusks which can be fed naturally by shrimp; the said compound feeds are juvenile shrimp or adult shrimp containing protein over 40 percent. To adopt this feeding mode of the present invention can promote shrimp growth, improve feed conversion efficiency and avoid feed resource waste caused by selectivity.

The invention relates to an aquaculture method of shells, in particular to an inductive agent for inducing marine bivalve mollusk to produce eggs as well as an application method thereof. The application method comprises the following steps: taking cooked egg yolk liquid obtained through bolting-silk treatment as the inductive agent, splashing the inductive agent in a parent shellfish pool uniformly according to the concentration, being 1.0 to 1.5 g/m<3>, of the cooked egg yolk under the conditions that the water temperature is 20 to 30 DEG C, the salinity is 25 to 35 and the pH value is 7.5 to 8.3, treating for 0.5 to 1 hour, performing pool separation according to a siphonage method after the parent shellfishes lay eggs, fully filling the pool with water again, and diluting the concentration of cooked egg yolk. Compared with other stimulation methods, the method has the following advantages: the egg producing efficiency can be increased by 30 to 50 percent and the egg producing efficiency of the parent shellfishes can be effectively improved. The inductive agent can be applied to various marine bivalve mollusk such as hard-shelled clam, clam, short-necked clam, cyclina sinensis gmelin, bay scallop, chlamys farreri and patinopecten yessoensis.

The invention provides a sealed inflatable upwelling culture device for intermediate culture of bivalve mollusks. The device includes a culture pipe, a negative pressure pipe, filter nets, an inflatorand a water outlet pipe, wherein the culture pipe is the outermost layer of the whole device, a bottom end of the culture pipe is provided with the filter mesh, an upper end side wall of the culturepipe is provided with a water outlet hole, the negative pressure pipe is placed in the culture pipe, a bottom end of the negative pressure pipe is provided with the filter net, one end of the water outlet pipe is connected with the negative pressure pipe, the other end of the water outlet pipe is placed at the water outlet hole of the culture pipe, and the inflator is placed on the filter net inside the negative pressure pipe. The culture device is advantaged in that through the culture method, water and electricity resources are greatly saved, the growth rate of juvenile mollusks is 20% to 50% higher than the traditional method, the survival rate is over 90%, and the seawater and the bait can be saved more than 70%.

The invention belongs to the field of marine biology application, particularly relates to the field of shellfish molecular genetic breeding, and relates to an electrotransfection bivalve shellfish gene editing method, the concentration of recombinant plasmids is kept to be greater than or equal to 2 mu g/mu l, sgRNA and Cas9 protein are mixed into RNP (sgRNA and Cas9 protein complex) according to the volume ratio of 2: 1, the working concentration of the sgRNA is greater than or equal to 50 ng/mu l, the working concentration of the Cas9 is greater than or equal to 250 ng/mu l, the working concentration of the sgRNA is greater than or equal to 50 ng/mu l, and the working concentration of the Cas9 is greater than or equal to 250 ng/mu l; an electrotransfection method is applied to bivalve mollusk gene editing, the electric pulse condition is that the pulse interval is 1s, the number of pulses is 2, the electrode distance is 4mm, pulse voltage and pulse time are changed, the pulse voltage range is 50V-500V, and the pulse time is 100 microseconds-1ms. The method disclosed by the invention can provide a basis for bivalve gene function verification and gene breeding application, and has the advantages of simplicity in operation, low cost, easiness in popularization and the like.

The invention relates to a method for processing breeding water of tortoises, which adopts a biological cleaning mode by a biologic chain principle. Fresh water fry and fresh water snail mollusk and/or bivalve mollusk are bred in a breeding pond of tortoises, and the breeding water of tortoises can be cleaned by a food chain formed by excrement of the tortoises, food residues, the fresh water fry, the fresh water snail mollusk and/or the bivalve mollusk. The body length of the fresh water fry is less than 10 centimeters; the breeding density of the fresh water fry is 1-25 fish fry per square meter, and the fresh water fry can be one of or more than one of scaled fish fry, carp fry, grass carp fry, chub fry and goldfish fry. The fresh water snail mollusk can be one of or more than one of mudsnail, Shankeng snail and sand snail. The fresh water bivalve mollusk is clam. The method for processing breeding water of tortoises has the advantages of easy realization, easy operation, environmental protection and low processing cost, accords with new requirements of new agricultural and sideline aquaculture for green ecology, and is suitable for the technical field of tortoise breeding.

The invention relates to a method for inducing oviposition and spermiation of bivalve mollusks, and belongs to the field of aquaculture. The method for inducing oviposition and spermiation of the bivalve mollusks comprises the following specific steps of preparing 5-hydroxytryptamine with the concentration of 2mM by using filtered seawater; adding pure filtered seawater into a container in advance and increasing the temperature by 3-8 DEG C compared with the normal culture temperature for later use; selecting parent mollusks with mature gonads, cleaning, putting into a disc-shaped container, and adding the filtered seawater which is half of the width of the parent mollusks until the mollusks are opened; injecting the prepared 5-hydroxytryptamine solution into the gonads of the parent mollusks, and then putting the parent mollusks into the prepared container to wait for oviposition and spermiation. Within 30 minutes, the oviposition and spermiation rate of the parent mollusks is 75% or above; compared with a traditional method, the method for inducing oviposition and spermiation of the bivalve mollusks is not limited by time, and cross infection between families can be completely eradicated; meanwhile, spermiation and oviposition are consistent in step and short in duration time, and manual continuous attention to oviposition and spermiation is avoided.

A toxicant detection system comprises at least one watertight chamber containing a mollusk. Water to be screened is introduced into the chamber. The mollusk is preferably supported on a mounting structure which facilitates quick mounting of the mollusk in the device, comprising a releasable element affixed to the shell of the mollusk, e.g., a bolt which is screwed into a nut affixed to the mollusk's shell. A sensing apparatus is provided which includes a movable member supported in the chamber so that it engages the shell of the mollusk, and moves when the shell opens and closes. The sensing apparatus detects the position of the mollusk shell based on the position of the movable member. The sensing apparatus preferably includes a Hall effect transducer which co-acts with a magnet carried on the movable member. The system allows for accurate readings of the small movements of the mollusk shell which aid in detecting toxicants, and also provides for installation of mollusks of varying sizes, without the need to move components of the standard configuration of the chamber. Methods and systems for detecting or identifying a toxicant in water being screened are also described.

The invention provides an early warning method for paralytic saxitoxin in bivalve mollusks. Whether the risk that the content of paralytic saxitoxin exceeds the standard exists or not is determined by detecting the content of paralytic saxitoxin in digestive glands of the bivalve mollusks. According to the method, the digestive gland is used as a monitoring sample, in toxin extraction operation, compared with integral soft tissue used in a conventional detection method, homogenate grinding is easier, operation is easy and convenient, and time and labor are saved. The volume of a detection sample is smaller, and toxin extraction is more economical. The accumulation of the digestive adenosine toxin is quicker and the accumulation amount is high, so that the toxin is more easily and sensitively detected. Meanwhile, compared with monitoring of the toxic algae level in a water body and a shellfish organism biomarker, the digestive gland is used as a toxin monitoring sample, interference of other factors is small, and the shellfish toxin level can be reflected more directly.

The invention discloses an intelligent detection method for bivalve mollusk planktonic larvae based on deep learning. The intelligent detection method comprises the steps of bivalve mollusk planktonic larva microscopic image collection with ink dots, image preprocessing, planktonic larva data set making, application model training and obtaining and planktonic larva detection. According to the method, intelligent detection of the number and the shell length of bivalve planktonic larvae under the microscopic view is realized through a deep learning method, so that the workload and the working time of artificial microscopic observation of the larvae are reduced, and the working efficiency of larva observation in the aquatic product field is greatly improved.

The invention provides an open inflatable upwelling culture device for intermediate culture of bivalve mollusks. The device includes a culture pipe, filter nets and an inflator, wherein the culture pipe is the outermost layer of the whole device, two ends of the culture pipe are opened, the filter nets are two layers, the culture pipe is divided by the filter nets into an upper portion, a middle portion and a lower portion, the middle between the two layers of filter nets is the middle portion and is the culture area, the inflator is arranged at an upper end of the upper filter net, an upper end of the culture pipe is provided with a water outlet, and a lower end of the culture pipe is provided with a water inlet. The culture device is advantaged in that through the culture method, water and electricity resources are greatly saved, the growth rate of juvenile mollusks is 20% to 50% higher than the traditional method, the survival rate is over 90%, and the seawater and the bait can be saved more than 70%.

The invention provides a method for quickly evaluating the resistance of bivalve mollusks based on a tension index, which is a method for detecting the tension index without damage on the basis of not influencing the activity of a tested individual and quickly evaluating the resistance of scallops based on the tension index. According to the method, efficient and lossless tension detection is carried out on the scallops, and the obtained four tension indexes including the total force, the shell closing time, the average force and the maximum force of the scallops serve as the basis for rapidly evaluating the scallop resistance. Compared with the traditional method, the method provided by the invention can realize nondestructive living body detection, and compared with a concentricity detection method, the method provided by the invention has the characteristics of being more stable, accurate, simple, convenient, high in detection success rate and the like. In addition, the tension of the adductor muscle of the scallop is used as an index for rapidly evaluating the resistance of the scallop for the first time. The method provided by the invention can provide reference for scallop resistance index determination, and provides technical support for subsequent breeding of scallop stress-resistant varieties.