24 results about "Bivalve shell" patented technology

The invention discloses a method for progressively extracting and identifying microplastics in bivalve in a deep sea methane leakage area according to sizes and levels. According to the method provided by the invention, biological tissues are subjected to freeze drying and dehydration, and micro-plastics contained in bivalve mollusks in an extreme environment are extracted in a lossless and classified manner by using a plurality of experimental steps such as a pH staged enhanced enzyme-hydrogen peroxide mixed digestion solution, hierarchical progressive vacuum suction filtration, identification according to size advantages and the like. The invention aims to realize quantitative and qualitative analysis of full-size micro-plastic in the bivalve according to size ranges. The characterization method is suitable for exploring microplastics enriched in each tissue of bivalve in a deep sea extreme ecosystem, and is matched with a captured seabed shellfish bed, so that the time history and historical accumulation of microplastics absorbed by the whole extreme ecosystem are deduced, and the problem of large-scale sampling, extraction and analysis in a deep sea extreme environment is solved; and a scientific inspection strategy is provided for the influence of the microplastic pollution history in a specific sea area.

The invention discloses a composite inducer for promoting adhesion and metamorphosis of marine bivalve mollusk larvae and an application method of the composite inducer. The composite inducer for promoting adhesion and metamorphosis of the marine bivalve mollusk larvae comprises glutamine, glutamic acid, potassium chloride, calcium chloride and taurine, and the concentration ratio of the glutamine to the glutamic acid to the potassium chloride to the calcium chloride to the taurine is 1: 1: 2 * 10: 1.7 * 10: 4. According to the application, glutamine, potassium ions, calcium ions, glutamic acid and taurine are matched, the application effect of the glutamine, the potassium ions, the calcium ions, the glutamic acid and the taurine in the aspect of improving the attachment and metamorphosis rate of the shellfish larvae is defined, the technical blank of multi-component cooperative regulation and control of the attachment and metamorphosis of the shellfish at present is filled, a new solution is provided for bivalve shellfish fry production, and the application prospect is wide. The method is of great significance in promoting healthy development of the mariculture industry.

To provide an apparatus for opening a bivalve shell such as a scallop by simply placing it manually or the like and continuously cutting the joint between one shell and the adductor muscle. [Solution] The device of the present invention is composed of a loading and conveying section I, a two-sided cutting section II, a shell fixing and scallop adductor muscle / shell joining and cutting section III or a shell fixing and scallop adductor muscle / shell joining and cutting section III', and a processed shell discharge section IV. By placing a bivalve shell on a platform plate connected to the conveyor chain of the conveying section, the shell is pressed against the platform plate by a pressing mechanism above the conveyor and sent to the cutting mechanism downstream of the conveying section, where a two-sided opening is formed in the end of the shell so as to cut off the hinge, the shell is supported on the platform plate by a shell fixing unit or the like downstream of the opening formation position, and as the shell moves on the conveyor chain, a knife is inserted into the shell from one of the opening sides by a knife pushing unit, and the knife is advanced and retreated to cut the joint between the adductor muscle and the shell, this bivalve shell opening device.

The invention discloses a bivalve mollusc identification method combining molecular identification with three-dimensional vision. Firstly, a bivalve mollusc sample is dissected and divided into adductor muscle tissue and shell, DNA of the adductor muscle tissue is extracted, COI gene sequence is amplified and sequenced by PCR, and molecular tags of the bivalve mollusc sample are obtained; then, the shell of the shell is restored, photos of the shell of the shell at different angles are taken by a three-dimensional model shooting device, and the photos are processed to obtain a three-dimensional model of the bivalve mollusc sample; then, a model for classification and identification is obtained based on three-dimensional model data training, and finally, the three-dimensional model data of the shell sample to be tested is input into the model. The model can realize classification and identification of unknown shells containing only three-dimensional model data, and the classification accuracy rate is above 80%, which solves the relevant difficulties of identification of morphologically similar shells and non-invasive identification, and has practical application significance for improving the breeding and production efficiency of bivalve molluscs, as well as the identification of cryptic species in ecological detection.

The invention discloses a fish and shellfish symbiosis seaborne net cage culture system and method, and relates to the technical field of aquaculture, and the method comprises the following steps: arranging a culture net cage in an offshore sea area; fish fries are put into a surrounding net, bivalve shellfishes are put into a shellfish culture piece cage, and the shellfish culture piece cage is hung in the culture net cage; feeding bait irregularly, checking the integrity of the shellfish culture cage and the covering condition of attachments regularly, and making a treatment means according to the covering rate; in the later period of culture, the adult fishes with the body length exceeding a preset value are caught irregularly in a fishing mode, and after the culture period is finished, the remaining adult fishes are caught in a concentrated mode by increasing the height of the purse seine; the shellfish culture piece cage is lifted out of the water surface to collect adult shellfish. The culture system disclosed by the invention comprises a culture net cage and shellfish culture piece cages, wherein the shellfish culture piece cages are hung in a purse seine. According to the method, mutualism of marine fishes and bivalve shellfish can be achieved, the resource utilization rate and the culture benefits are improved, and diversified fishery development can be achieved.

The present invention relates to the technical field of farming aquatic animals, such as bivalve mollusks, for example, oysters or scallops. In particular, the present invention provides a device, system and method for farming mollusks. This invention improves production efficiency in the farming of mollusks, mainly bivalve mollusks, by handling the movement of said mollusks and defining a specific area for each individual. This entails an improvement in individual survival, improving farming density and reducing loss caused by death.

The utility model provides a device for rapidly and nondestructively detecting shellfish body size and adductor muscle characters. The device comprises an X-ray light source, a conveyor, an X-ray panel acquisition instrument and an image analyzer, the X-ray light source and the X-ray panel acquisition instrument are arranged in the X-ray detection box; the X-ray light source is positioned above the conveyor and is fixedly arranged at the top of the detection box; the X-ray panel acquisition instrument is fixedly arranged below the conveyor; the conveyor horizontally penetrates through two opposite side walls of the detection box and is fixedly connected to the base; and the image analyzer is connected with the X-ray panel acquisition instrument, generates an image and calculates the body size and adductor muscle traits of the detected object. According to the high-flux X-ray detection device provided by the utility model, the living body, rapid and non-destructive measurement of the body size and adductor muscle traits of bivalve mollusks can be realized at the same time.

This invention discloses a bivalve shell-opening device, comprising a frame, a pushing mechanism, a pressing mechanism, and a shell-opening mechanism for separating the two shells of a bivalve. The pushing mechanism is equipped with a clamping and positioning mechanism to push the bivalve between the pressing mechanism and the shell-opening mechanism. The method includes the following steps: S1, feeding: The bivalve is placed with its connecting part facing down in the clamping and positioning mechanism, which clamps the bivalve. Then, the pushing mechanism pushes the clamping and positioning mechanism below the pressing mechanism; S2, shell opening: The pressing mechanism presses down, causing the bivalve to move towards the shell-opening mechanism. The shell-opening mechanism inserts into the connecting part of the bivalve, then opens the bivalve and separates the two shells. This invention improves automation, increases shell-opening efficiency, and reduces manual intervention.

The application provides a kind of dynamic evaluation method for carbon storage of bivalve, which comprises the following steps: monitoring and collecting environmental data of typical aquaculture sea area, calculating a functional function, carrying out on-site flow experiment to determine physiological and biological parameters of bivalve, converting to obtain carbon parameters, based on dynamic energy budget theory, combining multiple environmental factors and carbon parameters to construct a carbon budget model integrating the whole physiological process, and finally simulating the carbon storage flux of bivalve in the growth cycle. The application solves the problems of static evaluation of bivalve carbon sink, insufficient consideration of environmental factor fluctuation, incomplete description of key physiological processes and insufficient precision of carbon budget quantification, and realizes the daily dynamic and accurate evaluation of bivalve carbon storage, quantifies the carbon storage contribution of shell formation and biogenic sedimentation.

The application provides an antibacterial peptide and application thereof in juvenile shellfish culture, and the amino acid sequence of the antibacterial peptide is SEQ ID NO:2. The antibacterial peptide screened by the application is derived from bivalve shellfish, has less toxic and side effects on the shellfish, is suitable for playing a role in the shellfish, and has specificity and pertinence to pathogenic bacteria in the shellfish culture water. The amino acid sequence of the antibacterial peptide is optimized, has strong bacteriostatic activity, high stability, and high expression efficiency in yeast. After high expression of the antibacterial peptide, the antibacterial peptide is prepared into microcapsules with a particle size suitable for filtering and eating of the juvenile shellfish, so that the problems of the prior art, such as direct spraying into the culture water to cause the juvenile shellfish to be unable to eat, and low expression efficiency of microalgae and low antibacterial peptide content, are solved.

The purpose of the present invention is to provide a method for introducing exogenous nucleic acid fragments into bivalve sperm, wherein the method comprises centrifuging the collected bivalve sperm to remove the supernatant, adding NaCl solution to mix, centrifuging again and removing the supernatant; adding CaCl2 solution to the precipitate after removing the supernatant and mixing it by blowing; then adding the exogenous nucleic acid fragment and performing an ice-water bath; the solution after the ice bath is immediately heat-shocked under 42°C water bath conditions; the solution after the transformation is completed is then ice-water bathed again to complete the operation of introducing the exogenous fragment into the sperm. Experiments have shown that the introduction rate of the sgRNA recombinant expression plasmid into the body of the mud clam embryo after successful fertilization can reach 47% to 70%. Compared with the prior art, the introduction method of the present invention is simple to operate, has low introduction cost, short operation time, can realize batch operation, and meets the actual demand for large spawning of shellfish.

The present application belongs to the technical field of gene editing, and particularly relates to a gene editing method for obtaining a bivalve adult editing body. Specifically, a target gene with an outward visible phenotype and an early development not lethal is taken as a marker, target sites sgRNAs are designed based on the marker, and then a Cas9 / sgRNAs editing body is constructed by means of an electrotransfection system, so that gene editing of the bivalve is realized and the adult editing body is obtained. The present application breaks through the technical bottleneck that the existing gene editing of the bivalve is limited to the larva development stage and the editing body is difficult to screen in adulthood, and provides a new technical system for creating the editing body germplasm of the bivalve. In addition to being applicable to oysters, the technical system is also applicable to other bivalves with small fertilized eggs, difficult microinjection and high larva culture mortality.

The present invention discloses a method for intelligent detection of bivalve planktonic larvae based on deep learning, comprising: collecting microscopic images of bivalve planktonic larvae with ink dots, image preprocessing, preparation of a planktonic larvae data set, training and acquisition of an application model, and planktonic larvae detection. The method uses a deep learning method to achieve intelligent detection of the number and shell length of bivalve planktonic larvae under a microscopic field of view, thereby reducing the workload and working time of manual microscopic observation of larvae, and greatly improving the work efficiency of larvae observation in the aquatic field.

Systems and methods are providing for filtering a body of water, such as a lake, ocean, river or the like. A system comprises a housing having an interior configured for containing a plurality of bivalves within the water and a fluid distribution system coupled to the housing and configured to circulate water within the interior of the housing such that the water is filtered by the bivalves at a rate of about 10 to about 100 gallons of water per day per bivalves. The system provides a controlled environment to allow bivalves, such as clams, oysters and scallops, to filter the water naturally and remove materials, such as sediment, nitrogen, phosphorus, carbon, magnesium, zinc and the like. Oysters may, for example, filter pollutants from the water, remove excess nitrogen from the water and / or remove carbon from the water, which reduces the overall amount of carbon that eventually ends up in the atmosphere.

To provide an active substance having a function of enhancing an attachment metamorphosis rate of umbo later-stage larva of scallop, oyster, ark shell, etc., and inducing attachment metamorphosis, and a use method thereof.SOLUTION: An attachment metamorphosis enhancing method of bivalve which includes scallop, oyster, and ark shell includes adding glucose, D-fucose, D-xylose, D-galactose, D-mannose, gluconic acid, L-rhamnose, and glucosamine which are navicula active substance metabolite polysaccharides to a breeding water body in a culture tank for eyespot larva of the bivalve.SELECTED DRAWING: Figure 1

This invention relates to the field of food nutritional quality assessment technology, and discloses a method for predicting the lipid nutritional quality of bivalve mollusks based on climate change driving factors. The method includes: determining the family to which the bivalve mollusks to be predicted belong and their geographical region; obtaining the change values ​​of climate change driving factors in the region, including temperature, aragonite saturation, salinity, and dissolved oxygen; determining the lipid nutritional quality indicators to be assessed, and constructing a prediction model based on the correlation between the lipid nutritional quality indicators corresponding to different geographical regions and different bivalve mollusks and the change values ​​of climate change driving factors; substituting the obtained change values ​​of climate change driving factors into the prediction model to calculate the predicted values; and assessing the lipid nutritional quality indicators of the bivalve mollusks to be predicted based on the predicted values ​​and outputting the results. This invention reveals the response mechanism of mollusks' lipid metabolism to climate factors, providing objective data support for the site selection assessment and suitable species screening for bivalve mollusks.

The invention provides a bivalve shell opening and closing behavior identification method. A to-be-recognized image is acquired, the to-be-recognized image is input into a trained bivalve shell detector, and whether at least one target bivalve shell exists in the to-be-recognized image or not is detected according to the bivalve shell detector. And if not, returning to execute acquisition of the to-be-identified image, and if yes, acquiring a target bivalve mollusk set. And inputting a to-be-recognized image into the trained open bivalve mollusk instance divider, detecting the to-be-recognized image according to the open bivalve mollusk instance divider, and obtaining an initial open bivalve mollusk set. And carrying out matching processing on the target bivalve mollusk set and the initial open bivalve mollusk set, and obtaining a shell opening and closing behavior identification result of the to-be-identified image. Therefore, on the basis of the bivalve detection result, the bivalve shell opening and closing behavior recognition is realized in combination with the open bivalve shell instance segmentation result, and the accuracy of bivalve shell opening and closing behavior recognition is improved.

The present invention provides a method for rapidly and non-destructively evaluating the soft weight of bivalve molluscs, and belongs to the technical field of shellfish breeding. The method comprises: measuring the body size and weight traits of the bivalve molluscs in the training set, calculating the shell surface area of ​​the bivalve molluscs in the training set, constructing a soft weight estimation model based on the bivalve molluscs in the training set by using ridge regression, measuring the body size and full wet weight of the bivalve molluscs in the test set, calculating the shell surface area of ​​the bivalve molluscs in the test set, and estimating the soft weight of the bivalve molluscs in the test set by using the soft weight evaluation model. The present invention can accurately and non-destructively measure the soft weight of bivalve molluscs with high throughput, circumvents the problem that the traditional anatomical measurement method must sacrifice the bivalve molluscs, overcomes the limitations of the evaluation method based on image recognition, which is high cost, low efficiency, and unstable accuracy, and provides a solution to the difficult problem of dynamically monitoring the changes in the soft weight of bivalve molluscs, making it possible to systematically analyze the genetic basis of the regulation of the soft weight of bivalve molluscs and accelerate the cultivation of high-fat bivalve varieties.

The invention belongs to the technical field of shellfish testing, and discloses a high-flux bivalve shellfish shell closing force measuring system and method.The high-flux bivalve shellfish shell closing force measuring system comprises a shell closing force measuring module and a pressure measuring module, and the shell closing force measuring module is connected with the pressure measuring module through a multi-channel signal collector; the shell closing force measuring module comprises a bottom plate and a fixing device mounted above the bottom plate, and a plurality of pressure sensors are mounted on the fixing device; and each pressure sensor is electrically connected with the multi-channel signal collector. According to the technical scheme, operation is convenient, and shell closing force detection can be efficiently and massively carried out in a short time.

The present invention discloses a navicula active substance for promoting the attachment and metamorphosis of bivalve mollusks and its application. The navicula active substance is a polysaccharide of the active metabolite of navicula, which includes glucose, D-fucose, D-xylose, D-galactose, D-mannose, etc. The raw materials for its preparation are ecological and pollution-free. The prepared polysaccharide is non-toxic, and the preparation method is convenient and fast. It has been experimentally confirmed that the polysaccharide of the active metabolite of navicula can improve the attachment and metamorphosis rates of several late-veliger larvae such as scallops, oysters and ark clams, has the effect of inducing the attachment and metamorphosis of bivalve mollusks, has a high metamorphosis efficiency, can improve the economic benefits of bivalve mollusk farming, and has good application prospects.

The invention belongs to the technical field of gene editing, and particularly relates to a gene editing method for obtaining a bivalve adult edit. The method specifically comprises the following steps: by taking a target gene which has a visual phenotype in appearance and is not lethal in early development as a marker, designing a targeting site sgRNAs based on the marker, and constructing a Cas9 / sgRNAs edit body by virtue of an electrotransfection system, so as to realize gene editing of bivalve mollusks and obtain an adult edit body. According to the method, the technical bottlenecks that the existing bivalve mollusk gene editing is only limited to the larva development stage and the edit adult screening is difficult are broken through, and a new technical system is provided for the creation of bivalve mollusk edit germplasm materials. In addition to oysters, the technical system is also suitable for other bivalve shellfishes with small fertilized eggs, difficulty in micro-injection and high larva culture death rate.

The present invention provides a method for producing DHMBA, an antioxidant, from the flesh of bivalves of the superfamily Oenothera, such as pearl oysters or oysters, by heating or pressurizing the flesh of the pearl oysters or oysters, in a larger quantity and at a higher concentration than other bivalves. [Solution] The present invention is characterized in that 3,5-dihydroxy-4-methoxybenzyl alcohol can be produced in a larger quantity from the heat-treated bivalve meat of the Bivalviidae superfamily than from other bivalves by placing the meat of a bivalve belonging to the Bivalviidae superfamily into an extract and heating the extract.

To provide a device for opening a shell by easily mounting a bivalve such as scallop by manual work, and continuously cutting a connection part between one shell and an adductor muscle.SOLUTION: A device for opening a bivalve includes: a mounting conveyance part I; a two-face cut part II; a shell fixing and adductor muscle / shell connection-cutting part III or an adductor muscle / shell connection-cutting part III'; and a processed shell discharge part IV. Due to that a bivalve is mounted on a base plate joined onto a conveyor chain of the conveyance part, the bivalve is sent to a cutting mechanism at a conveyance downstream side while being pushed to the base plate by a pushing-down mechanism of a conveyor upper part. An opening of two faces is formed on a shell end part so as to cut off a hinge, the shell is supported on the base plate by a shell fixing unit or the like at an opening formation position downstream side, a knife is inserted into the shell from one opening face by a knife push-in unit while the shell moves due to movement of the conveyor chain, and the knife is progressed and retreated so as to cut a connection part between the adductor muscle and the shell.SELECTED DRAWING: Figure 3

The invention discloses a bivalve mollusk transport case and a using method. The bivalve mollusk transport case comprises an outer transport case and an inner storage case. The storage inner box is nested in the transportation outer box; a gap is reserved between the storage inner box and the transportation outer box; the storage inner box further comprises a sponge plate, and the sponge plate is soaked with water and placed in the storage inner box. Wherein a cooling cavity is formed in a gap between the transportation outer box and the storage inner box, and the cooling cavity is used for injecting cooling water. Therefore, according to the bivalve mollusk transport case, the low-temperature water in the water injection layer of the bivalve mollusk transport case is used for keeping the storage inner case at a low temperature, sufficient humidity can be provided for the storage inner case by soaking the sponge plate, bivalve mollusks are put into the storage inner case to be transported, a large amount of water does not need to be relied on any more, light-weight transport is achieved, and meanwhile the transportation efficiency is improved. As a large amount of water does not need to be injected into the storage inner box, the space utilization rate of the transport box is increased, and the effects of reducing cost and increasing efficiency are achieved.