48 results about "Bivalve shell" patented technology

Provided by this invention are novel intergeneric bivalve shellfish hybrids, including clams and scallops. Also provided are methods for producing the novel hybrids and their progeny.

The invention discloses a purity method for heavy metal in the bivalve shellfish. The purity method is: culturing the bivalve shellfish temporarily in the sand leach natural sea water that is 22 to 28 Deg.C of the water temperature, 12 to 32 per micrometer salinity, and continuous charge, releasing microalgae into the sand leach natural sea several times for purity treatment for 2 to 31 days. Purifying bivalve shellfish with the present invention, the heavy metal can be discharged 40 to 50 percents, which meets the edible hygienic standards.

An anti-swallow pacifier includes: a base member having nipple member protruding forwardly from the base member, a bivalve shell having a pair of half-shell pieces hingedly secured to the base member having a restoring spring retained on the base member for resiliently automatically closing the two half-shell pieces for covering the nipple portion, an anti-swallow shield circumferentially formed on a rear portion of the base member for preventing from swallow of the pacifier by a baby as the shield may be obstructed beyond the baby's mouth without being swallowed, and a handle secured to the anti-swallow shield.

The invention provides a bivalve processing device which can easily realize high functionalization. The bivalve processing device can utilize a forcedly-opening mechanism (8) to open an unprocessed bivalve (2), then takes out the edible part which can be eaten uncooked from the lower shell (2b) under the forcedly-opening mechanism (8), and is provided with a foreign body removing mechanism (6) which is arranged on the upstream side of the forcedly-opening mechanism (8) along the shell transportation direction and is used to remove the foreign bodies, which is attached on the surface of the upper shell (2a) and abut on an upper absorbing lining (61).

The invention relates to a Japanese scallop sea area breeding method which comprises the following steps: monitoring germen indexes to parent scallops of Japanese scallops in a sea area in a propagate season; monitoring the quantity and the size of baby scallops in the sea area having the baby scallops of the Japanese scallop after the monitoring data show the oviposition, and putting a breeding device into the set bearing area when the monitoring of the number and the size of the baby scallops shows the proportion of adhering baby scallops: bivalve shell baby scallops with shell height more than 200 micrometers occupy more than 40 percent of the total of bivalve shell baby scallops, or the bivalve shell baby scallops with shell height more than 230 micrometers occupy more than 20 percent of the total of the bivalve shell baby scallops, or the bivalve shell baby scallops with shell height more than 250 micrometers occupy 5-10 percent of the total of the bivalve shell baby scallops. The Japanese scallop sea area breeding method can obtain natural degeneration-resistant excellent baby scallops in an open sea area, realizes the massive production of the natural baby scallops, and develops a source of the natural green baby scallops.

A chair seat includes an upper metallic covering sheet, a lower covering sheet, and a main body; the upper metallic covering sheet is substantially in the shape of a bivalve shell valve, and the main body is held in the upper metallic covering sheet; the upper metallic covering sheet is pressed at a lower circumferential portion thereof to form a compressed step-shaped section, which closely touches and grips the main body; the lower covering sheet is substantially in the shape of a bivalve shell valve, and has a holding portion, and the upper metallic covering sheet is tightly fitted in the holding portion of the lower covering sheet at the compressed step-shaped section such that the main body is wrapped, and such that the upper metallic covering sheet is separable from the lower covering sheet for replacement.

The invention provides a breeding method for lowering the bacterial quantity and heavy metal content in bodies of bivalve molluscs, and belongs to the technical field of purification of bivalve molluscs. The breeding method comprises the following steps that chlorinated disinfectant and seawater are mixed and disinfected for 10-14 hours to obtain disinfected seawater, wherein the effective chlorine content of the chlorinated disinfectant is 40-60 wt%, and the ratio of the mass of the chlorinated disinfectant to the volume of the seawater is (600-1350)g:(20-45)m<3>; the obtained disinfected seawater is mixed with sodium thiosulfate, and the obtained mixed solution is subjected to continuous aeration for 10-14 hours to obtain de-chlorine seawater, wherein the mass ratio of the sodium thiosulfate to the chlorinated disinfectant is 1:(1-3); the bivalve molluscs are bred in the de-chlorine seawater for 10-35 days and are fed with microalgae biological bait in the breeding period. By the adoption of the breeding method for lowering the bacterial quantity and heavy metal content in the bodies of the bivalve molluscs, the bacterial quantity and the heavy metal content in the bodies of thebivalve molluscs can be lowered, both the total number of bacteria and the number of coli groups reach the food hygiene standard, and obtained oyster products have fresh meat, do not have fishy smellor mucus, and are milky white.

A metamorphosis inductor based on organic substances for the larvae of dual-shell shellfish is prepared from KCl (5.0-1000 g/m3, L-tyrosine (0.01-3 g/m3 and VB12 (0.01-0.5 mg/m3 through proportionally mixing. Its advantage is high metamorphosis percentage increased by 20-40%.

The invention discloses a method for simultaneously detecting AZA1, AZA2 and AZA3 in bivalve aquatic products. According to the method, liquid-liquid extraction and hybrid anion exchange reversed phase adsorption solid-phase extraction and purification are utilized to perform sample treatment on scallops, oysters, variegated clams and other bivalve aquatic products, and an analysis and detection method for simultaneously determining three main azaspiracid shellfish poisons AZA1, AZA2 and AZA3 in a sample through a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method is established; and furthermore, the average recovery rate of AZAs is more than 80%, and the relative standard deviation (RSD) is less than 12%. The method disclosed by the invention has the characteristics of high speed, high efficiency, sensitivity and accuracy.

The invention discloses a water pollution monitoring biosensor, a monitoring system and a monitoring method, wherein the biosensor comprises an aquatic organism which is a living bivalve organism, anoptical grating fixing structure, and an optical grating; the optical grating fixing structure comprises a first fixing base, a second fixing base and an elastic beam piece, the elastic beam piece isof an arch structure, a first fixing part and a second fixing part of the elastic beam piece are mounted on the first fixing base and the second fixing base respectively, and a bent part in the middleof the elastic beam piece is located between the two fixing bases; the two fixed bases are respectively fixed on the surfaces of two shells of the aquatic organism; the optical grating is a fiber Bragg grating and is adhered to the outer side of the bent part of the optical grating fixing structure; opening and closing of the two shells of the aquatic organism drive the bent part to expand or contract through the two fixing bases, then the optical grating is driven to regularly expand or contract, and signal changes are generated.

The invention belongs to the field of marine biology application, particularly relates to the field of shellfish molecular genetic breeding, and relates to an electrotransfection bivalve shellfish gene editing method, the concentration of recombinant plasmids is kept to be greater than or equal to 2 mu g/mu l, sgRNA and Cas9 protein are mixed into RNP (sgRNA and Cas9 protein complex) according to the volume ratio of 2: 1, the working concentration of the sgRNA is greater than or equal to 50 ng/mu l, the working concentration of the Cas9 is greater than or equal to 250 ng/mu l, the working concentration of the sgRNA is greater than or equal to 50 ng/mu l, and the working concentration of the Cas9 is greater than or equal to 250 ng/mu l; an electrotransfection method is applied to bivalve mollusk gene editing, the electric pulse condition is that the pulse interval is 1s, the number of pulses is 2, the electrode distance is 4mm, pulse voltage and pulse time are changed, the pulse voltage range is 50V-500V, and the pulse time is 100 microseconds-1ms. The method disclosed by the invention can provide a basis for bivalve gene function verification and gene breeding application, and has the advantages of simplicity in operation, low cost, easiness in popularization and the like.

The invention discloses an outdoor large-scale spat intermediate culture method. The method comprises the following steps that 1) an outdoor shellfish bed is constructed in a pond; 2) a water level height difference is gradually formed among a higher-place pond, an algae pond and the outdoor shellfish bed in the vertical direction, high-density shrimp culture is carried out in the higher-place pond, fish and shrimp mixed culture is carried out in the algae pond, and intermediate culture on spats is carried out in the outdoor shellfish bed; and 3) culture wastewater is purified: the culture wastewater in the higher-place pond is discharged into the algae pond for ectopic dilution and conversion firstly, the wastewater is changed into fertilizer water to promote the growth of beneficial unicellular algae, then the fertilizer water in the algae pond is introduced into the outdoor shellfish bed, the fertilizer water is changed into purified water through the filter feeding activity of bivalve shellfish, and meanwhile, the unicellular algae are used as bait organisms to promote the growth of the spats, so that the culture wastewater is purified in an ex-situ manner. According to the method, the breeding cost is greatly reduced, the productivity of intermediate culture of the spats is greatly improved, high economic benefits are achieved, the problem of treatment of culture wastewater can be effectively solved, and the method is suitable for popularization and application in areas suitable for breeding of bivalve spats such as clams and razor clams.

The invention relates to a method for inducing oviposition and spermiation of bivalve mollusks, and belongs to the field of aquaculture. The method for inducing oviposition and spermiation of the bivalve mollusks comprises the following specific steps of preparing 5-hydroxytryptamine with the concentration of 2mM by using filtered seawater; adding pure filtered seawater into a container in advance and increasing the temperature by 3-8 DEG C compared with the normal culture temperature for later use; selecting parent mollusks with mature gonads, cleaning, putting into a disc-shaped container, and adding the filtered seawater which is half of the width of the parent mollusks until the mollusks are opened; injecting the prepared 5-hydroxytryptamine solution into the gonads of the parent mollusks, and then putting the parent mollusks into the prepared container to wait for oviposition and spermiation. Within 30 minutes, the oviposition and spermiation rate of the parent mollusks is 75% or above; compared with a traditional method, the method for inducing oviposition and spermiation of the bivalve mollusks is not limited by time, and cross infection between families can be completely eradicated; meanwhile, spermiation and oviposition are consistent in step and short in duration time, and manual continuous attention to oviposition and spermiation is avoided.

The invention relates to the fields of biology and material science, in particular to a preparation method of a scanning electron microscope sample of a bivalve shell. The method comprises the following four parts of pre-processing, positioning, sampling and post-processing. The preparation method of the scanning electron microscope sample of the bivalve shell is provided by utilizing common experiment equipments in a laboratory. By adopting the method provided by the invention, the scanning electron microscope sample of the bivalve shell can be prepared quickly, simply and efficiently, a clear scanning electron microscope picture of the bivalve shell can be effectively obtained.

The invention provides a method for feeding bivalve with bivalve feeding agent, which comprises the steps of using cellobiose and stored sugar or the sugar forming stored sugar with a molecular weight less than 540 as the bivalve feeding agent and solving the bivalve feeding agent in the bivalve feeding environment till the concentration is 10mg/l to 30000mg/l.

The invention belongs to the technical field of marine pollutant stress research and relates to a buried bivalve mollusk running water type marine pollution stress experiment system. The experiment system comprises a water storage tank, a premixing tank, an experiment tank and a purification tank which are connected in sequence, as well as a peristaltic pump, a valve and an instrument device for automatic control which are arranged on a connecting pipeline, wherein a central fixing frame is arranged in the premixing pool, a seawater inlet, a mother liquor sample adding opening and an oxygenation stone are arranged in the middle of the central fixing frame, the seawater inlet is connected with a water outlet of the water storage pool, a mother liquor sample adding opening is connected witha mother liquor bottle, an oxygenation stone is connected with an oxygenation device air outlet pipe, a cover body for sealing the experiment pool is arranged at the top of the experiment pool and provided with an experiment pool water outlet connected with the purification pool, an experiment pool water inlet connected with the premixing pool is formed in the bottom of the experiment pool, a submersible pump is arranged on an inner wall of the premixing tank below the central fixing frame, and a dissolved oxygen probe is set at an outlet of the experimental pool. The experimental system provides technical support for accurately researching the influence of marine pollutants on shellfish.

The invention provides an early warning method for paralytic saxitoxin in bivalve mollusks. Whether the risk that the content of paralytic saxitoxin exceeds the standard exists or not is determined by detecting the content of paralytic saxitoxin in digestive glands of the bivalve mollusks. According to the method, the digestive gland is used as a monitoring sample, in toxin extraction operation, compared with integral soft tissue used in a conventional detection method, homogenate grinding is easier, operation is easy and convenient, and time and labor are saved. The volume of a detection sample is smaller, and toxin extraction is more economical. The accumulation of the digestive adenosine toxin is quicker and the accumulation amount is high, so that the toxin is more easily and sensitively detected. Meanwhile, compared with monitoring of the toxic algae level in a water body and a shellfish organism biomarker, the digestive gland is used as a toxin monitoring sample, interference of other factors is small, and the shellfish toxin level can be reflected more directly.

The invention discloses an intelligent detection method for bivalve mollusk planktonic larvae based on deep learning. The intelligent detection method comprises the steps of bivalve mollusk planktonic larva microscopic image collection with ink dots, image preprocessing, planktonic larva data set making, application model training and obtaining and planktonic larva detection. According to the method, intelligent detection of the number and the shell length of bivalve planktonic larvae under the microscopic view is realized through a deep learning method, so that the workload and the working time of artificial microscopic observation of the larvae are reduced, and the working efficiency of larva observation in the aquatic product field is greatly improved.

The invention discloses a method for detecting bivalve derived components by fluorescent quantitative PCR (polymerase chain reaction), which comprises the following steps: firstly, weighing a sample, carrying out DNA (deoxyribonucleic acid) extraction on the sample, carrying out PCR amplification through a primer, then carrying out agarose gel electrophoresis on the amplified product to obtain an electrophoretic band, cutting and recycling the target band, sending the target band to a sequencing company for sequencing, inputting a sequencing result into an NCBI (National Center of Biotechnology Information) website for BLAST (Basic Local Amplified Sequence Test) comparison, species information and related target gene sequences are obtained, the target gene sequence of each bivalve shellfish is downloaded, DNAMAN software is used for comparison to obtain the same gene sequence of the obtained shellfish variety, primers and Taqman probes are designed according to the target sequences, the primers and the Taqman probes are sent to a company to synthesize the primers and the probes, commercially available shellfish samples are used for primer and probe verification, and the target gene sequence of each bivalve shellfish is obtained. The bivalve shellfish can be detected. According to the present invention, the DNA extraction is performed on the sample, the real-time fluorescence quantitative PCR amplification is performed by using the specific primer probe, whether the sample contains the bivalve shellfish component is determined according to the amplification curve and the CT value, and the type detection can be effectively performed on the shellfish component in the food.