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Method for inducing oviposition and spermiation of bivalve mollusks

A technology for bivalve molluscs and shells, which is applied in the field of aquaculture and can solve problems such as time-consuming, labor-intensive, low success rate, and unstable egg production

Pending Publication Date: 2021-07-06
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional method of induction of shellfish includes methods such as drying in the shade, which is time-consuming and laborious in the actual operation process. There are technical problems such as long duration of induction, low success rate, unstable spawning amount, and out-of-sync spawning time. It requires a long time of staff. Observation, and it is very prone to cross-contamination between families, which directly affects the process of family selection

Method used

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  • Method for inducing oviposition and spermiation of bivalve mollusks
  • Method for inducing oviposition and spermiation of bivalve mollusks

Examples

Experimental program
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Effect test

Embodiment 1

[0020] A method for inducing philippine clams to lay eggs and ejaculate, the specific steps are as follows:

[0021] 1) The filtered seawater uses the seawater from the growth area of ​​Philippine clams. After sand filtration and disinfection, connect the 0.22μm filter element with a syringe, push the seawater out of the filter element, and prepare 5-HT (5-hydroxytryptamine) with a concentration of 2mM with the filtered seawater. Store at 4°C and protect from light.

[0022] 2) Add filtered seawater to a 1L transparent small bucket (spawning bucket) in advance, heat the water bath and raise the temperature to 7-8°C higher than normal seawater, and rise to 30±1°C, so that the broodstock to be produced can be put into the small bucket .

[0023] 3) 30 Philippine clam broodstocks with mature selective glands were washed and placed in a dissecting tray, and filtered seawater with a depth of 1 cm was added to the dissecting tray, and the shells were opened (it took about 3 hours f...

Embodiment 2

[0028] In order to further prove the effect of serotonin to induce labor, four groups of experiments were set up in this embodiment, and the ovulation and oviposition of Philippine clams were counted in the first 80 minutes (statistics were performed once every 10 minutes). The results are shown in figure 1 and figure 2 . Group A: heating seawater + injecting 5-HT; group B: heating seawater + injecting seawater; group C: not heating seawater + directly injecting 5-HT; group D: not heating seawater + injecting seawater. The ratio of individual ovulation and oviposition within 20 minutes in experimental group A was the highest, reaching 53.2%, which was much higher than that of group C (16.7%) without heating seawater. Neither group B nor D oviposited. At the end of the experiment, the total induced labor rate in group A reached 85.3%, that in group C was 75.0%, and that in groups B and D was less than 5%. The results showed that, for the two experimental groups A and C injec...

Embodiment 3

[0030] A method for inducing Pacific oyster to lay eggs and ejaculate, the specific steps are as follows:

[0031] 1) Filtered seawater uses seawater from the Pacific oyster growth area. After sand filtration and disinfection, connect the 0.22μm filter element with a syringe, push the seawater out of the filter element, and prepare 5-HT (5-hydroxytryptamine) with a concentration of 2mM with the filtered seawater. 4 Store at ℃ and avoid light.

[0032] 2) Add filtered seawater to a 1L transparent small bucket (spawning bucket) in advance. The normal temporary water temperature for Pacific oyster broodstock is 18-20°C, and the heated water temperature for induced oyster production is 22-23°C, so that the broodstock that is ready to give birth can be released. into the bucket.

[0033] 3) 30 broodstocks with mature selective glands were washed and placed in a dissecting tray. Added filtered seawater with a depth of 3cm to the dissecting tray and waited for their shells to open (...

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Abstract

The invention relates to a method for inducing oviposition and spermiation of bivalve mollusks, and belongs to the field of aquaculture. The method for inducing oviposition and spermiation of the bivalve mollusks comprises the following specific steps of preparing 5-hydroxytryptamine with the concentration of 2mM by using filtered seawater; adding pure filtered seawater into a container in advance and increasing the temperature by 3-8 DEG C compared with the normal culture temperature for later use; selecting parent mollusks with mature gonads, cleaning, putting into a disc-shaped container, and adding the filtered seawater which is half of the width of the parent mollusks until the mollusks are opened; injecting the prepared 5-hydroxytryptamine solution into the gonads of the parent mollusks, and then putting the parent mollusks into the prepared container to wait for oviposition and spermiation. Within 30 minutes, the oviposition and spermiation rate of the parent mollusks is 75% or above; compared with a traditional method, the method for inducing oviposition and spermiation of the bivalve mollusks is not limited by time, and cross infection between families can be completely eradicated; meanwhile, spermiation and oviposition are consistent in step and short in duration time, and manual continuous attention to oviposition and spermiation is avoided.

Description

technical field [0001] The invention belongs to the field of aquaculture, and in particular relates to a method for inducing bivalve molluscs to lay eggs and ejaculate, so as to carry out artificial insemination and family construction. Background technique [0002] Buried shellfish live in soft-bottom habitats such as sediment, and have water pipes and well-developed axopodia. Common buried shellfish include Philippine clams, green clams, meretrix meretrix, clams, razor clams, mud clams, and clams. The depth of burial varies with the length of the water pipe. These buried bivalve molluscs have delicious meat and high economic value, and the contradiction between supply and demand has become increasingly prominent. With the further expansion of domestic and foreign markets, domestic shellfish farming production has increased, and the farming area has continued to expand. With the continuous improvement and development of my country's shellfish farming industry, shellfish ...

Claims

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Application Information

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IPC IPC(8): A01K61/54
CPCA01K61/54Y02A40/81
Inventor 孙秀俊吴磊张天时刘志鸿周丽青吴彪田吉腾
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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