The invention discloses a plant wound healing protective agent, which consists of the following components in percentage by weight: 0.1 to 8 percent of bactericide, 0.1 to 11 percent of nutrient, 40 to 85 percent of protective agent, 1 to 6 percent of pigment, 2 to 20 percent of tackifier, and 8 to 20 percent of water. The invention also discloses a preparation method of the protective agent, which comprises the following steps of: firstly, adding water and stirring; secondly, adding the nutrient and tackifier with stirring, heating the solution to 30 to 60 DEG C, and stirring for 10 to 50 minutes; thirdly, adding the pigment with stirring, and after continuously stirring for 10 to 60 minutes, stopping heating; and finally, adding the bactericide, after stirring for 10 to 40 minutes, adding the protective agent, and after stirring for 60 to 240 minutes, stopping stirring. The plant wound healing protective agent has good effect on the infection caused by Cytospora, canker, gummosis and other fungus and bacteria pathogenic bacteria, and has special effect on the protection of rot scars of fruit trees, canker scars, gummosis scars and wounds and saw bites.