The invention discloses a micro-membrane calcium fruit sunburn protective agent as well as preparation and application methods thereof, and relates to the technical field of plant protection. The micro-membrane calcium fruit sunburn protective agent is prepared from the following raw material components in parts by weight: 40-80 parts of zinc oxide, 10-20 parts of nanoscale calcium carbonate, 1-1.5 parts of a penetrating agent, 1-1.5 parts of a thickening agent, 3-5 parts of a spreader and 80-120 parts of deionized water. Zinc oxide can effectively absorb and scatter ultraviolet rays, relieveburn of crop leaves, stems, stamens, fruits and the like caused by excessively strong direct light, reduce the surface temperature of the fruits, and meanwhile, do not influence photosynthesis of theleaves; the nano calcium can enter crops through the surface layers of the crops, supplement calcium, prevent physiological diseases caused by nutrient deficiency of the crops, improve the quality offruits, increase the hardness of the fruits and effectively prolong the storage time of the fruits. A layer of protective film is formed on the surfaces of fruits after the micro-membrane calcium fruit sunburn protective agent is sprayed, fruit sunscald is effectively prevented and treated, and the commodity rate of the fruits is greatly increased; the use is simple and convenient, the use amountis small, the effect is direct, the effect is obvious, the labor cost expenditure is reduced, and good economic benefits and wide market prospects are realized.