Sensor for detecting plant active small molecules and preparation method

A small molecule and sensor technology, applied in the field of plant sensors, can solve the problems of difficult detection of internal biological signals in stems and leaves of plants, inappropriateness, high requirements for reliability, transparency, photosynthesis of stems and leaves, etc.

Active Publication Date: 2021-05-28
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the existing sensor devices are attached to the plant epidermis, which can only detect the information at the plant-environment interface such as the temperature and humidity of the epidermis, but it is difficult to detect the internal biological signals of the stems and leaves of the plant, such as the concentration of active small molecules, etc.
In addition, the existing sensors have the function of penetrating plant epidermis, and they directly contact tissue fluid to realize biosensing. However, most of them are developed based on animal transdermal sensing requirements, and there are still many problems to be solved when they are directly applied to plant sensing For example, the electrode array is not suitable for the thinner leaves and curved stems of plants due to its non-flexibility. For example, animal transdermal sensing requires high biological safety of sensing electrodes, while plant transdermal sensing requires Reliability such as resistance to high temperature / high humidity environment, resistance to direct sunlight, and sensor transparency (does not hinder the photosynthesis of stems and leaves) requires high requirements
Therefore, plant sensors at this stage still have relatively large defects.

Method used

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  • Sensor for detecting plant active small molecules and preparation method
  • Sensor for detecting plant active small molecules and preparation method
  • Sensor for detecting plant active small molecules and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] see figure 1 As shown, the embodiment of the present invention provides a sensor 10 for detecting plant active small molecules, including:

[0062] Microneedle array electrode 11, which is used to detect the concentration of plant active small molecules;

[0063] A transparent flexible substrate 12, one side of which has a groove 121 for fixing the microneedle array electrode 11;

[0064] A transparent flexible film 13, which is fixed on the other side of the transparent flexible substrate 12 away from the groove 121;

[0065] A plurality of signal conduction circuits 14, which are arranged between the transparent flexible substrate 12 and the transparent flexible film 13, and each of the signal conduction circuits 14 passes through the transparent flexible substrate 12 and the microneedle array electrode 11 connect.

Embodiment 2

[0067] On the basis of Embodiment 1, this implementation may also include the following:

[0068] see Figure 1 to Figure 5 As shown, the microneedle array electrode 11 includes a microneedle array fixed on a plant and a base on which the microneedle array is fixed, and the microneedle array is formed by a plurality of microneedle arrays. Correspondingly, the groove 121 of the transparent flexible substrate 12 has a structure for fixing the substrate. In one application scenario, the cross section of the groove 121 of the transparent flexible substrate 12 is T-shaped, and the microneedle array is arranged in the middle of one side of the substrate. In another application scenario, the bottom of the groove 121 of the transparent flexible substrate 12 has a through hole, so that the two sides of the transparent flexible substrate 12 are communicated through the through hole. The flexible substrate 12 is flexible, so the microneedle array electrode 11 can be installed and fixed...

Embodiment 3

[0071] On the basis of Embodiment 1, this embodiment may also include the following:

[0072] An embodiment of the present invention provides a method for preparing the above-mentioned sensor, and the microneedle array electrode 11 is prepared by the following method:

[0073] Prepare zinc oxide (ZnO) microsphere solution;

[0074] Prepare the mold of the microneedle array electrode;

[0075] The zinc oxide microsphere solution is mixed with the first mixed solution for preparing microneedle array electrodes to prepare a second mixed solution;

[0076] Filling the second mixed solution into the mold of the microneedle array electrode and solidifying;

[0077] After the second mixed solution is solidified, the zinc oxide microspheres are removed by acid etching to obtain a porous microneedle array electrode;

[0078] The porous microneedle array electrode is modified to obtain a microneedle array electrode including a working electrode, a counter electrode and a reference el...

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Abstract

The invention relates to a sensor for detecting plant active small molecules and a preparation method, and the sensor comprises: a microneedle array electrode, which is used for detecting the concentration of the plant active small molecules; a transparent flexible substrate, one side of which is provided with a groove for fixing the microneedle array electrode; a transparent flexible film, which is fixed on the other side, far away from the groove, of the transparent flexible substrate; and a plurality of signal conduction circuits, which are arranged between the transparent flexible substrate and the transparent flexible film, wherein each signal conduction circuit penetrates through the transparent flexible substrate and is connected with the microneedle array electrode. The sensor can be attached to the surfaces of leaves, stems and the like of a plant, has dual functions of sensing and fixing, and can enable visible light to irradiate the surface of the plant through the sensor.

Description

technical field [0001] The invention belongs to the field of plant sensors, in particular to a sensor for detecting plant active small molecules and a preparation method. Background technique [0002] Rhizosphere microorganisms are of great significance in the process of plant growth, and quite a variety of rhizosphere microorganisms are beneficial to plant growth. For example, bean vegetables can symbiotically fix nitrogen with the corresponding rhizobia, thereby reducing the input of nitrogen fertilizers, so that bean vegetables not only occupy an important position in vegetable supply, bring huge economic value to farmers, but also contribute to reducing agricultural non-point sources. Pollution makes a substantial contribution. [0003] The different response mechanisms of rhizosphere microorganisms to plant growth promotion and stress will be fed back at levels such as hormones. However, the current standard detection methods are in vitro detection of destructive plan...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/3277Y02A40/10
Inventor 谢曦刘繁茂谢致平胡宁杭天陈惠琄陶军李湘凌黄新烁刘星星刘子淇周灵菲
Owner SUN YAT SEN UNIV
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