Feeding device suitable for sogatella furcifera injection method RNA interference experiment and using method thereof

Abstract

The invention discloses a feeding device suitable for a sogatella furcifera injection method RNA interference experiment. The feeding device comprises a transparent plastic bottle and a matrix layer used for culturing rice; the matrix layer is located at the bottom in the transparent plastic bottle; an opening is formed in a the transparent plastic bottle body, a door body capable of preventing sogatella furcifera from flying out is hinged to the position, located on the opening, of the bottle body, an active carbon layer and a magnetic lock used in cooperation with the opening are arranged on the door body, a sampling opening is formed in the door body, and a sampling door is hinged to the sampling opening. When the feeding device is in use, the door body is opened, sogatella furcifera receiving specific gene dsRNA is put into the transparent plastic bottle from the door body, and the door body is closed; after 24-h culture, the phenotypic change of sogatella furcifera is observed, sogatella furcifera is sampled through the sampling opening at the interval of 24 h for 4-5 times, experiment results are recorded. The device is reasonable in structure and method setting and more suitable for using injection RNA interference for researching gene functions of sogatella furcifera.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to the technical field of auxiliary tools for studying the gene function of the important rice pest white-backed planthopper, and more particularly to a feeding device suitable for the RNA interference experiment of the white-backed planthopper injection method and a use method thereof. Background technique [0002] Rice is an important food crop in my country, and its output ranks first among various food crops. The white-backed planthopper belongs to the order Homoptera and is a pest of the planthopper family. It not only stings and eats rice, thereby endangering the normal growth of rice, but also spreads plant viruses, causing paralysis of rice plants and lodging, and rice yield reduction or even failure. Studying the function of each gene of S. furcifera from the essence of S. furcifera, that is, at the gene level, will help the research and control of this pest. RNA in...

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Problems solved by technology

[0003] Since the discovery of RNAi technology, it has been widely used in the study of insect gene function, but there is no report on the gene function study technology for white-backed planthopper.

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