230 results about "RNA interference" patented technology

The invention discloses three pattern recognition protein Hc beta GRP genes for preventing and treating fall webworms, dsRNA and application thereof. Specifically, the nucleotide sequences of the three pattern recognition protein Hc beta GRP genes for preventing and treating fall webworms are respectively SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3. The recombinant Hc beta GRP protein is successfully obtained through a prokaryotic expression system, an in-vitro bacteriostatic test proves that the recombinant Hc beta GRP protein, the Hc beta GRP protein and the dsRNA have direct bacteriostatic activity, dsRNA is designed based on a pattern recognition protein Hc beta GRP gene, a hyphantria cunea Hc beta GRP dsRNA interference system implemented by using an injection method is established, an obvious gene silencing effect is obtained by injecting the Hc beta GRP dsRNA interference system into hyphantria cunea, and the Hc beta GRP dsRNA interference system is applied to the hyphantria cunea. The technical support is provided for the application of the Hc beta GRP RNAi technology. Besides, the dsRNA of the hyphantria cunea Hc beta GRP is combined with the biocontrol bacteria by utilizing an established hyphantria cunea Hc beta GRP RNA interference system, so that the control effect of the biocontrol bacteria can be remarkably improved.

The present disclosure relates to isolated nucleic acid molecules comprising at least two double-stranded inhibitory ribonucleic acid (RNA) molecules adapted to silence the same or different genes by RNA interference to enhance silence, thereby modulating gene expression.

The invention relates to a key gene GqBurscon for wing development of a Qinghai grassland moth and application thereof. The key gene GqBurscon for the wing development of the Qinghai grassland caterpillar has a nucleotide sequence as shown in SEQ ID NO: 1. According to the dsRNA synthesized by the key gene GqBurscon for wing development of the Qinghai grass caterpillar, one chain of the dsRNA is the same as the sequence of SEQ ID NO: 1, and the other chain of the dsRNA is reversely complementary with the sequence of SEQ ID NO: 1. The key gene GqBurscon for the wing development of the Qinghai grassland is used for synthesizing dsRNA, the synthesized dsRNA is used for carrying out RNA interference on a target gene, the gene transcription level is knocked down, the gene is effectively silenced in chest, abdomen, wings and wing base tissues of male adult Qinghai grassland, the length and width of the wings are correspondingly reduced, the development of the wings is limited, and the gene transcription level is reduced. The flying capability is influenced, and the death is caused.

The invention discloses dsRNA of a targeted potato beetle heat shock protein HSP70-KDa3 gene as well as a preparation method and application of the dsRNA of the targeted potato beetle heat shock protein HSP70-KDa3 gene. In order to solve the problems that an existing RNAi target gene is prone to off-target, poor in field stability and the like, the potato beetle heat shock protein gene LdHSP70-KDa3 serves as an RNA interference target for the first time, and a double-stranded RNA molecule shown as SEQ ID NO: 1 and a preparation method for preparing the double-stranded RNA are provided. Feeding experiments prove that the double-stranded RNA can efficiently silence target genes, the survival rate of potato beetle larvae is remarkably reduced, and feeding and growth development of the potato beetle larvae are inhibited. In addition, the invention further provides a biological preparation containing the double-stranded RNA and application of the biological preparation in prevention and treatment of potato beetles. The dsRNA targeting the HSP70-KDa3 gene provided by the invention has the characteristics of high efficiency, specificity, environmental friendliness and the like, and a new technical scheme is provided for green prevention and control of potato beetles.

The invention discloses a VdNOB1 gene as well as an anti-pathogenic bacterium target gene segment, an interference vector and disease-resistant application thereof. According to the invention, the VdNOB1 gene is subjected to knockout mutation, so that the infection pathogenicity of verticillium dahliae to host plants can be obviously reduced. The invention further provides a verticillium dahliae VdNOB1 gene anti-pathogenic bacterium target gene segment, the nucleotide sequence of the verticillium dahliae VdNOB1 gene anti-pathogenic bacterium target gene segment is shown as SEQ ID No.4, and the pathogenicity of verticillium dahliae to host plants can be remarkably reduced after plants are transformed by an RNA interference vector constructed by the verticillium dahliae VdNOB1 gene anti-pathogenic bacterium target gene segment. The invention further provides an RNA (Ribonucleic Acid) interference vector containing the VdNOB1 gene or an anti-pathogenic bacterium target gene fragment thereof. The verticillium dahliae VdNOB1 gene or the anti-pathogenic-bacterium target gene segment and the RNA interference vector thereof have application prospects in the aspects of improving the resistance of crops to diseases caused by verticillium dahliae, cultivating new varieties of verticillium dahliae-resistant plants and the like.

The present disclosure provides double stranded oligonucleotides, compositions, and methods relating thereto. The present disclosure encompasses the recognition that structural elements of double stranded oligonucleotides, such as base sequence, chemical modifications (e.g., modifications of sugar, base, and / or internucleotidic linkages) or patterns thereof, and / or stereochemistry (e.g., stereochemistry of backbone chiral centers (chiral internucleotidic linkages), and / or patterns thereof, can have significant impact on oligonucleotide properties and activities, e.g., RNA interference (RNAi) activity, Ago2 loading, thermal stability, in vivo stability, delivery to tissues and into cells, etc. The present disclosure also provides methods for treatment of diseases, e.g., hepatic diseases, central nervous system (CNS) diseases, etc., using provided double stranded oligonucleotide compositions, for example, in RNA interference.

The invention discloses a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention relates to the technical field of biology, and provides a method for preventing and treating cotton verticillium wilt based on RNAi (RNA interference) as well as related components and application thereof. Specifically, the invention discloses application of the verticillium wilt prevention and treatment by a method for inhibiting or silencing the expression of verticillium dahliae pathogenic gene VdHDAC (coding protein of the verticillium dahliae pathogenic gene VdHDAC is shown as SEQ ID NO: 3), the core of the verticillium wihliae pathogenic gene VdHDAC is to provide dsRNA molecules capable of targeting the gene, and the positive-sense strand of the dsRNA molecules is preferably selected from SEQ ID NO: 5, SEQ ID NO: 8, SEQ ID NO: 9 or SEQ ID NO: 11. Based on the molecule, the invention further provides a recombinant vector containing the coding sequence, a recombinant microorganism, a transgenic disease-resistant plant and an RNAi pesticide preparation. The scheme can be realized through host-induced gene silencing, microorganism-mediated gene silencing or preparation treatment and the like, the virulence of pathogenic bacteria can be effectively reduced, and a new technical approach is provided for green prevention and control of verticillium wilt.

The invention discloses dsRNA for targeted regulation of a freshwater shrimp PKA-R1 gene and an obtaining method and application of the dsRNA, and belongs to the technical field of biology.Firstly, the freshwater shrimp PKA-R1 gene is obtained, then a gene segment with the sequence of SEQ ID NO: 3 is obtained through RNA interference and other technologies, dsPKA-R1 is synthesized through the gene segment, and after the synthesized dsPKA-R1 is injected into the pericardial cavity of a freshwater shrimp, it is proved that the dsPKA-R1 can slow down growth of the freshwater shrimp through growth parameter comparison.

The invention relates to an application of a FaPDHE1alpha gene or an encoding protein thereof in prevention and control of strawberry gray mold, and belongs to the field of plant genetic engineering and the field of prevention and control of postharvest diseases of fruits and vegetables. The invention discloses a key positive regulation and control effect of FaPDHE1alpha in regulation and control of strawberry gray mold resistance. By constructing a 35S:: FaPDHE1alpha overexpression vector and a FaPDHE1alpha RNAi interference vector, according to transient gene overexpression and RNA interference silencing tests, it is verified that high expression of the FaPDHE1alpha gene can obviously enhance the resistance of strawberry fruits to botrytis cinerea, and silencing of the gene causes reduction of the disease resistance of the fruits. Mechanism studies show that after the FaPDHE1alpha gene is activated, mitochondrial energy metabolism and controlled ROS signal generation are promoted, antioxidant enzyme activity is enhanced, and defense related gene expression is induced, so that the disease resistance of fruits is improved. By means of functional genomics, the key function of the FaPDHE1alpha gene in prevention and control of gray mold after strawberry harvest is illuminated for the first time, and the FaPDHE1alpha gene can be used for screening disease-resistant breeding materials or developing biological prevention and control preparations for postharvest diseases and has important application value.

The invention belongs to the field of biological agriculture, and particularly relates to application of a wheat stripe rust effect protein gene HASP 38 in improvement of stripe rust resistance of wheat. The amino acid sequence of the wheat stripe rust effect protein HASP 38 provided by the invention is as shown in SEQ ID NO: 1, the nucleotide sequence of the wheat stripe rust effect protein gene HASP 38 is as shown in SEQ ID NO: 2, and an RNA interference vector of the gene is provided. According to the invention, an RNA (Ribonucleic Acid) interference vector of the gene is transformed into a Fielder wild type material, so that the HASP 38 gene silencing wheat with resistance to stripe rust CYR32 is obtained. According to the invention, the wheat stripe rust effect protein gene HASP 38 is determined to play a negative regulation role in wheat stripe rust resistance, and a stripe rust resistant wheat material is created by using the negative regulation factor, so that a new gene and germplasm resource are provided for wheat stripe rust resistant breeding.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an application of SlERF098 protein and a coding gene thereof in regulating and controlling steroid alkaloid content to influence botrytis cinerea resistance. According to the application, the resistance of tomatoes to gray mold is enhanced by regulating and controlling the metabolism of steroid glycoalkaloid through the coding gene. The tomato SlERF098 gene is positioned through whole genome association analysis, and an overexpression strain and an RNA interference strain of the tomato SlERF098 gene are constructed. Experiments prove that the SlERF098 can obviously activate the expression of the GAMEs gene in the SGAs metabolic pathway. In addition, overexpression of the SlERF098 gene can improve the content of SGAs metabolites in tomatoes and the resistance to gray mold, while RNA interference strains present an opposite trend. The invention provides a new target for research on disease-resistant molecular breeding and plant immunity-metabolism coordinated regulation and control, and has important agricultural application value.

This invention relates to the fields of molecular biology and biomedicine, and discloses siRNA for the expression of the *Schistosoma japonicum* Hsc20 gene and its applications. The siRNA contains a nucleotide sequence that hybridizes to a target sequence of the *Schistosoma japonicum* Hsc20 gene, wherein the target sequence is selected from the sequences shown in SEQ ID NO. 1-SEQ ID NO. 4. The siRNA specifically inhibiting the expression of the *Schistosoma japonicum* Hsc20 gene provided by this invention can be used to interfere with the transcription and expression of the *Schistosoma japonicum* Hsc20 gene and the growth and development of *Schistosoma japonicum*. In vitro experiments have confirmed that the siRNA provided by this invention can efficiently silence the *SjHsc20* gene; and in vivo RNA interference experiments in mice show that this siRNA can induce 50.62% (…) P The insect reduction rate was <0.05% and 44.29% ( P A liver oocyte reduction rate of <0.01% is suitable for preparing drugs to treat schistosomiasis.

This invention discloses a method for preventing attacks by the star longhorn beetle. AcAttacin The dsRNA of the gene and its applications, as described AcAttacin The nucleotide sequence is shown in SEQ ID NO: 1. A *D. spp.* longhorn beetle ds. was successfully constructed using injection and feeding methods. AcAttacin RNA interference (RNAi) system, for AcAttacin The expression of the beetle exhibits highly efficient inhibition; using this system, the longhorn beetle... AcAttacin The combined application of this insect biocontrol bacterium, *Metarhizium anisopliae*, significantly enhances the control efficacy of *Metarhizium anisopliae* against the Asian longhorn beetle. This invention not only lays a solid theoretical foundation for the development of green, environmentally friendly, precise, and highly effective immunosuppressants for the Asian longhorn beetle, but also opens up a new strategic path for my country to achieve its green and sustainable development goals for the Asian longhorn beetle. It has extremely important significance and broad application prospects in ecological protection and forestry production.

Provided herein are a PPP1R15B RNAi agent, and a composition comprising a PPP1R15B RNAi agent. Also provided herein are methods of reducing the expression of PPP1R15B in a subject and / or treating a metabolic disease associated with PPP1R15B in a subject using the PPP1R15B RNAi agent or a composition comprising the PPP1R15B RNAi agent.

This invention discloses a pathogenic gene of barley stripe bacterium. Pg00232 Its application. Barley stripe disease is a fungal disease caused by *Barley Stripe Pathogen*, which seriously affects barley yield and quality. Traditional control methods have limited effectiveness and pose environmental risks; breeding disease-resistant varieties is the best strategy. This invention provides pathogenicity information for *Barley Stripe Pathogen*. Pg00232 Genes, and obtained through RNA interference technology Pg00232 The mutant strain further confirmed that Pg00232 The influence of genes on the growth, development, and pathogenicity of barley stripe causal agent. This discovery provides technical support for subsequent large-scale screening of pathogenic mutants of barley stripe causal agent, promotes the isolation, identification, and pathogenic mechanism research of pathogenic genes, and contributes to the breeding and selection of disease-resistant varieties.

The invention discloses a nucleic acid loading system based on phase separation protein as well as a preparation method and application of the nucleic acid loading system. The system comprises a protein NONO and a long-chain non-coding RNA LincGET which interacts with the protein NONO. NONO and LincGET form a droplet structure under an in-vitro phase separation condition, the droplet structure can be further combined with a nucleic acid fragment capable of being combined with LincGET to form a complex with a cavity structure, and the nucleic acid fragment is loaded in a cavity region. The preparation method comprises the following steps: expressing and purifying NONO protein, transcribing LincGET RNA in vitro, mixing and incubating in a buffer system capable of initiating phase separation of NONO protein and LincGET RNA to form liquid drops, and adding a target nucleic acid fragment to realize loading. The system can be recovered into a solid structure after being digested by DNase I, and has a reversible loading characteristic. The invention provides a novel, controllable and specific nucleic acid loading platform which can be applied to delivery of nucleic acid drugs, gene editing tools and RNA interference molecules.

This invention discloses a method for inhibiting Tamdy virus infection and the use of TCP1 inhibitors. Specifically, this invention discloses a method for inhibiting Tamdy virus (TAMV) infection of host cells, the method comprising the step of reducing or inhibiting the expression level and / or activity of the TCP1 gene or its encoded protein in host cells, wherein the reduction or inhibition of the expression level and / or activity of the TCP1 gene or its encoded protein is achieved by methods selected from the group consisting of: gene mutation, gene knockout, gene interruption, RNA interference technology, gene editing technology, introduction of inhibitors of genes or proteins, or combinations thereof.

The present application relates to the field of insect control, and particularly relates to a SIRT1 gene of Rhopalosiphum padi and application thereof and a method for controlling Rhopalosiphum padi. The gene encodes a protein with an amino acid sequence as shown in SEQ ID NO: 2. The present application finds that the SIRT1 gene plays a role in the growth and development of Rhopalosiphum padi. Inhibition of the expression of SIRT1 can significantly increase the mortality rate of Rhopalosiphum padi nymphs, significantly reduce the proportion of winged aphids, and ultimately cause the population to decline and the toxin transmission ability to weaken. By using RNA interference technology, it is found that inhibition of the expression of the SIRT1 gene of Rhopalosiphum padi can significantly increase the mortality rate of the nymphs, and significantly reduce the proportion of winged adults after development. In addition, by spraying dsRNA of the SIRT1 gene interference sequence of Rhopalosiphum padi encapsulated by a nano material, the mortality rate of the Rhopalosiphum padi nymphs is significantly increased.

The invention discloses a method for regulating and controlling migratory locust embryo diapause by a BTB transcription factor LOCMI00260 and application of the method. According to the present invention, the full length of the BTB transcription factor LOCMI00260 is cloned from migratory locust, the specific double-stranded RNA interference sequence fragment dsRNA of the BTB transcription factor LOCMI00260 is developed, and the dsRNA of the BTB transcription factor LOCMI00260 is introduced into the migratory locust egg by using the microinjection method; the result shows that the migratory locust BTB transcription factor LOCMI00260 can improve the diapause rate of migratory locust. The invention provides a new means and thought for migratory locust prevention and treatment.

The present disclosure provides double stranded oligonucleotides, compositions, and methods relating thereto. The present disclosure encompasses the recognition that structural elements of double stranded oligonucleotides, such as base sequence, chemical modifications (e.g., modifications of sugar, base, and / or intemucleotidic linkages) or patterns thereof, and / or stereochemistry (e.g., stereochemistry of backbone chiral centers (chiral intemucleotidic linkages), and / or patterns thereof, can have significant impact on oligonucleotide properties and activities, e.g., RNA interference (RNAi) activity, Ago2 loading, thermal stability, in vivo stability, delivery to tissues and into cells, etc. The present disclosure also provides methods for treatment of diseases, e.g., hepatic diseases, central nervous system (CNS) diseases, etc., using provided double stranded oligonucleotide compositions, for example, in RNA interference.

RNA interference agents for inhibiting the expression of Factor XII (FXII) gene are described. Pharmaceutical compositions comprising one or more FXII RNAi agents together with one or more excipients capable of delivering the RNAi agent(s) to a liver cell in vivo are also described. Delivery of the FXII RNAi agent(s) to liver cells in vivo provides for inhibition of FXII gene expression and treatment of angioedema, including hereditary angioedema (HAE) and venous thromboembolism (VTE), and diseases associated with angioedema.

The present invention provides nucleic acid molecules for modulating the level or amount of mRNA of FGFR4. In particular, the present invention provides delivery of a primary microRNA to form a precursor after in vivo processing, as well as a microRNA for in vivo inhibition of expression of mRNA of FGFR4. The invention also provides a delivery system of the nucleic acid molecule, comprising a carrier, an exosome and a cell, and a pharmaceutical composition containing the same. The invention also provides the use of the nucleic acid molecule and delivery system in disease treatment and drug preparation, in particular methods and drugs for cancer.

The invention discloses application of a wheat disease-resistant factor TatrxH4 in prevention and treatment of gibberellic disease, and belongs to the technical field of genetic engineering, and a coding gene CDS sequence of the wheat disease-resistant factor TatrxH4 is shown as SEQ ID NO: 1; the amino acid sequence of the wheat disease-resistant factor TatrxH4 is as shown in SEQ ID NO: 2; the wheat disease-resistant factor TatrxH4 plays a positive regulation role in immune response of wheat to gibberellic disease, and over-expression of the wheat disease-resistant factor TatrxH4 improves the resistance of wheat to gibberellic disease. According to the invention, an agrobacterium tumefaciens-mediated method and an RNA interference technology are adopted to obtain a wheat strain of an overexpressed disease-resistant factor TatrxH4, and the disease-resistant factor TatrxH4 is identified according to the morbidity condition, disease index and expression condition of disease-resistant related marker genes of an overexpressed plant and common Fielder wheat infected with fusarium graminearum PH-1, so that the disease-resistant factor TatrxH4 is identified. The wheat disease-resistant factor TatrxH4 is known to play a positive regulation role in the immune response of wheat scab resistance, so that the wheat disease-resistant factor TatrxH4 can be used for creating a scab-resistant wheat germplasm material and plays a practical role in prevention and treatment of the wheat scab on the basis of the positive regulation role of the wheat disease-resistant factor TatrxH4.

Provided herein are SOD1 RNAi agents and compositions comprising a SOD1 RNAi agent. Also provided herein are methods of using the SOD1 RNAi agents or compositions comprising a SOD1 RNAi agent in reducing SOD1 expression and / or treating SOD1-mediated neurological disease.

The invention relates to the technical field of biological medicine, in particular to an oligonucleotide molecule for inhibiting expression of ACTRIIB and application of the oligonucleotide molecule. The oligonucleotide molecule comprises at least 12 contiguous nucleotides of any one of the nucleotide sequences of SEQ ID NO: 1 to SEQ ID NO: 70, SEQ ID NO: 141 to SEQ ID NO: 185, SEQ ID NO: 231 to SEQ ID NO: 244 and SEQ ID NO: 71 to SEQ ID NO: 140, SEQ ID NO: 186 to SEQ ID NO: 230, SEQ ID NO: 245 to SEQ ID NO: 258, or a sequence that differs from the at least 12 contiguous nucleotides by no more than 3 nucleotides, or a sequence complementary thereto, the nucleotides being in a modified or unmodified state. The oligonucleotide molecule mediates RNA interference on the ACTRIIB, so that muscle building, muscle function improvement, exercise capability enhancement and prevention or treatment of muscle system related diseases are realized.

The invention discloses application of a flammulina velutipes transcription factor gene AreA in regulation and control of cell wall polysaccharide synthesis and sporocarp taste. Flammulina velutipes as an important edible mushroom is widely welcomed due to rich nutrition, but the edible experience of consumers is seriously influenced and the market potential of the flammulina velutipes is limited due to the problems that the flammulina velutipes is poor in chewiness, easy to plug teeth and the like. The silent strain of the enoki mushroom areA gene is successfully constructed through an RNA interference technology, it is found that the polysaccharide content of cell walls and the toughness of sporocarp can be reduced through reduction of gene expression, then the taste of the sporocarp is improved, and the market competitiveness of the sporocarp is improved. According to the invention, the areA gene in the flammulina velutipes is effectively silenced through the specifically designed siRNA fragment, so that the polysaccharide content of the cell wall and the toughness of the sporocarp are reduced, and a theoretical basis is provided for reducing the toughness of the cell wall of the flammulina velutipes by using a genetic engineering technical means.

The application discloses a method for improving the resistance of citrus to Huangyanbing by using ClPsaN gene, comprising the following steps: (1) cloning a VIGS fragment of the citrus ClPsaN gene; (2) constructing two VIGS expression vectors of TRV and CLBV; and (3) transforming the VIGS expression vector into citrus to obtain VIGS plants in which the citrus ClPsaN gene is silenced. According to the method, the VIGS vector of the citrus ClPsaN gene is constructed, then the vector is transformed into citrus, and the obtained citrus plants can obviously show resistance to Huangyanbing. The titer of Huangyanbing, i.e., Huangyanbing yellow vein-illness virus, can be reduced by 22-34%, and the accumulation amount of virus protein can be reduced by 35-50%, so that the incidence of Huangyanbing is significantly reduced. In addition, the TRV silencing of the citrus ClPsaN gene does not affect the phenotype of the citrus plants, and the citrus ClPsaN gene can be used as a candidate gene to be used in the breeding of citrus plants resistant to Huangyanbing by using VIGS silencing, RNA interference and gene editing technologies in cooperation with multiple genes of the citrus plants resistant to or susceptible to Huangyanbing.

The present invention relates to products and compositions and their uses. In particular, the invention relates to nucleic acid products that interfere with the expression of the TMPRSS6 gene or inhibit its expression and to therapeutic uses such as for the treatment of hemochromatosis, porphyria and hematological disorders such as β-thalassemia, sickle cell disease and iron overload from transfusions or myelodysplastic syndrome.

The invention discloses an application of a CTL-S1 gene and miR-let-7 in regulation and control of reproductive development of bactrocera cucurbitae. The CTL-S1 gene has high specificity expression in bactrocera cucurbitae ovary, and the hatching rate of offspring can be reduced by inhibiting the expression of the CTL-S1 gene through RNA interference. Through the analysis of a dual luciferase report system of RNA co-immunoprecipitation, the result shows that the miR-let-7 and the CTL-S1 have a targeting relationship. Overexpression of miR-let-7 can lead to significant reduction of the expression quantity of the CTL-S1 gene, and a phenotype similar to RNA interference inhibition of the expression of the CTL-S1 gene appears, so that the hatching rate of offspring is reduced. The invention provides a potential gene target for developing a genetic control strategy for destroying the reproduction of bactrocera cucurbitae, provides a new thought for the subsequent biological control of insects by adopting a sterile technology, and has a wide application prospect.

Methods of preparation and use of modified double stranded oligonucleotides, oligonucleotide conjugates or compositions are provided. The invention provides a modified double-stranded oligonucleotide, specific modification of a single site or multiple sites on specific sites of a positive-sense strand and / or an antisense strand of the oligonucleotide can enable the modified oligonucleotide to have good pharmaceutical activity, and the modified double-stranded oligonucleotide has good application prospects in RNA interference (RNAi) drug development.