Pest repellent containing methyl palmitate and preparation and use methods thereof
A technology of methyl hexadecanoate and pest repellent, which is applied in the directions of animal repellent, botanical equipment and method, pest repellent, etc., to achieve the effects of realizing pollution-free control, avoiding control difficulties and high efficiency
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[0031] A preparation method of a pest repellent includes the following steps:
[0032] (1) Preparation of mother liquor A: accurately weigh 0.42~4.26 g methyl hexadecanoate and dissolve it with a solvent;
[0033] (2) Preparation of auxiliary liquid B: prepare 10% sodium dodecylbenzene sulfonate solution with deionized water;
[0034] (3) Take 10 mL of B solution and mix with A solution, and mix well with ultrasonic for 4-6 min;
[0035] (4) Use a solvent to dilute the volume to 1 L to obtain the pest repellent.
[0036] The method of using the pest repellent is to dilute the pest repellent with ethanol or water by 0 to 5 times, and spray it directly on the flower buds and branches of the plant.
[0037] The pests are beet armyworm and / or cotton bollworm.
[0038] The said beet armyworm is a female beet armyworm.
[0039] The pest repellent of the present invention can be used for the prevention and control of beet armyworm, cotton bollworm and other pests, and has the best repellent effec...
Example Embodiment
[0044] Example 1: Discovery of pest repellent substances
[0045] (1) Feeding method of beet armyworm
[0046] Place the collected mature larvae of Spodoptera exigua into a 250 mL beaker lined with moist sand and seal with gauze. After pupation and emergence, the adults are transferred to another 250 mL beaker. Supplement nutrition with 10% honey water to lay eggs and hatch on healthy Chinese rose leaves, and raise them to mature larvae (feeding conditions: 27℃, 70% RH, 12 h photoperiod). The pupae were pupated in the same way, and the pupae were taken out under a stereoscope to distinguish males and females. The male and female pupae were raised separately and used for biological testing after emergence.
[0047] (2) The inhibitory effect of host plant infection with powdery mildew on the oviposition behavior of adults of beet armyworm
[0048] Put about 3 cm of sterile soil in 2 500 ml beakers, and sprinkle an appropriate amount of sterile water to moisten the soil. Select healthy...
Example Embodiment
[0056] Example 2: Separation and identification of repellent active ingredients
[0057] The headspace dynamic adsorption method was used to extract the volatile component mixture of Chinese rose leaves infected with powdery mildew, and the mixture was analyzed and tested by gas chromatography-antenna electrophysiology (GC-EAD). The results are as follows image 3 , Figure 4 , Shown in Table 1. The monomer compounds in the mixture that can produce obvious EAD signals were further identified by GC-MS. Among them, peak No. 5 is methyl hexadecanoate, which has a relatively large content in the mixture and produces a strong EAD signal. The results showed that the natural compound (mixture) extracted from the susceptible rose plants contains methyl hexadecanoate (monomer), which may be used by the beet armyworm female moth to identify the susceptible host during the selection process of the egg laying site One of the important information compounds.
[0058] Table 1 Volatile componen...
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