The invention discloses a preparation method and application of a mass-selective monoclonal antibody capable of resisting 8 triazin type agricultural chemicals, and belongs to the technical field of biology. According to the preparation method disclosed by the invention, an appropriate activity arm is introduced into a 1,3,5- triazine ring, immune half-antigen and coating half-antigen are prepared, by an activity ester method, ovalbumin (OVA) and bovine serum albumin (BSA) are coupled to prepare immunogen and coating antigen, a Balb / c mouse is immunized by the immunogen, and a monoclonal antibody is prepared through a hybridoma technique; based on the antibody, the coating antigen is used as detection antigen, heterogenous indirect competitive enzyme-linked immunosorbent assay (ELISA) is established, and the mass-selective monoclonal antibody can be used for detecting 8 triazin type agricultural chemicals including prometryn, prometon, mesoran, secbumeton, atratone, desmetryn, simetrynand ametryn. The method is low in detection cost, high in sensitivity and simple and convenient in operation, and is suitable for on-site quick massive screening of the 8 triazin type agricultural chemicals.