13results about "Metallothioneins" patented technology

The invention relates to a method for preparing a nano metal iron oxide material from iron ion binding protein with specific binding capacity, and belongs to the technical field of genetic engineering. In order to overcome the technical problems of non-specific binding of metallothionein and iron ions and relatively low production efficiency in a traditional nano # imgabs0 # biological preparation method, the invention provides an iron ion binding protein with specific binding capacity, and a specific binding iron ion polypeptide sequence is connected on the basis of metallothionein, so that the iron ion binding protein with specific binding capacity is obtained. The specific binding amount of iron ions is increased, and the specific binding iron ion protein is used as a template to prepare the # imgabs 1 # nano material. According to the method for preparing the nano-metal iron oxide material through specific binding of the iron ion protein, reaction conditions are mild, # imgabs2 # is prepared and synthesized in an oriented mode through high selectivity of the specific binding iron ion protein, the preparation process is easy to control, and the particle size range of the prepared # imgabs3 # is 20-1000 nm.

To provide a bis-linkage method for producing a conjugate of a cell-binding agent and a cytotoxic drug molecule in a specific manner.SOLUTION: A method for producing a conjugate compound of Formula (I-v) or Formula (I-w), wherein two or more residues of a cell-binding agent / molecule are simultaneously or sequentially reacted with a readily reactive bis-linker compound.SELECTED DRAWING: Figure 1

The invention relates to a construction method of a metallothionein yeast surface display engineering strain capable of efficiently enriching copper ions. According to the method, the metallothionein yeast surface display engineering strain is constructed based on a saccharomyces cerevisiae cell surface display technology. According to the method, a metallothionein gene CUP1 after codon optimization is connected to a saccharomyces cerevisiae expression vector pYD1 through the steps of double digestion, connection, transformation and the like, and a recombinant display plasmid pYD1-CUP1 is successfully constructed. A lithium acetate method is adopted to convert the recombinant display plasmid pYD1-CUP1 into tryptophan auxotroph saccharomyces cerevisiae EBY100, a plurality of recombinant transformants are obtained through MD plate screening, and screening and verification are carried out. The engineering yeast strain constructed by the method can improve the display efficiency of metallothionein on the surface of saccharomyces cerevisiae cells and enhance the enrichment capacity of the engineering strain on copper ions, so that inorganic copper is replaced by copper-enriched yeast, the side effect of copper is reduced, and the absorption and utilization rate of livestock and poultry on copper is improved.

The present invention relates to a genetically controlled nanoscopy contrast-generating unit comprising a metal interactor, wherein the metal interactor is compatible with nanoscopy fixation protocols, nanoscopy post-fixation protocols, and nanoscopy metal staining protocols and wherein the metal interactor is a molecule to which metal ions can bind to or react with. The present invention also relates to a genetically controlled structural element, wherein said genetically controlled structural element organizes the genetically controlled nanoscopy contrast-generating unit. The genetically controlled structural element can be an encapsulin and the genetically controlled nanoscopy contrast-generating unit can be one or two murine metallothionein-3, or three chimeric metallothioneins. The present invention also relates to a genetically controlled scaffold, wherein said genetically controlled scaffold spatially organizes the genetically controlled structural elements. The present invention also relates to the use of such genetically controlled nanoscopy contrast-generating unit, such genetically controlled structural element, and / or such genetically controlled scaffold for nanoscopy detection methods. The present invention also relates to a nanobiomaterial consisting of the isolated genetically controlled structural elements, and / or genetically controlled scaffolds. The present invention also relates to vectors comprising a nucleic acid encoding a genetically controlled nanoscopy contrast-generating unit, a genetically controlled structural element, and / or a genetically controlled scaffold.

The application discloses a method for improving the cadmium ion adsorption capacity of Escherichia coli, and belongs to the technical field of genetic engineering. E. coli BL21 to Cd 2+ , and the unit point mutation of T16C, K19C and S45C to CeMT2 can significantly improve the Cd 2+ binding capacity of CeMT2, and the cell Cd 2+ concentration of the T16C, K19C and S45C mutants is increased by 1.32 times, 1.50 times and 1.41 times, respectively. E. coli The recombinant expression of CeMT2 (K19C) and LmCadC in BL21 can obviously improve the cadmium ion adsorption capacity of Escherichia coli, and the maximum adsorption capacity reaches 8.5 mg / g.

A Pichia pastoris fermentation process and its application belong to the field of microbial fermentation technology. The technical problem to be solved is to provide an optimized Pichia pastoris fermentation process to address the shortcomings of existing technologies, such as complex purification of soluble MT, low yield, and high price. The key points of the technical solution are a Pichia pastoris fermentation process, including the following steps: (1) transferring Pichia pastoris carrying the MT gene to YPD plate medium for culture and activation to obtain the cultured strain; (2) inoculating the cultured strain obtained in step (1) into BMGY medium with an initial pH of 7-9 for shaking culture, and obtaining cell cells after centrifugation; (3) waiting for the OD of the cell cells obtained in step (2) 600 At 6-10, the medium was transferred to BMMY medium and induced metal was added to induce MT expression; (4) The process parameters were further optimized using a BP neural network model.

The present application belongs to the technical field of plant genetic engineering, and particularly relates to a gene combination expressed in plants in series and application thereof in improving the cadmium tolerance of plants. The gene combination expressed in plants according to the present application comprises a PPKS gene, an MT2aS gene and an MT2bS gene; the nucleotide sequences of the PPKS gene, the MT2aS gene and the MT2bS gene are respectively shown as SEQ ID NO. 2, SEQ ID NO. 4 and SEQ ID NO. 6. The present application improves the heavy metal enrichment capacity and tolerance of plants to heavy metals including cadmium by expressing the gene combination in plants, not only improves the heavy metal absorption capacity of plants near the rhizosphere, but also avoids the toxicity of excessive absorption of heavy metals to plants, thereby improving the tolerance of plants to heavy metals including cadmium.

The invention discloses a method for preparing a nano metal iron oxide material from a metallothionein mutant strain, and belongs to the technical field of gene engineering. In order to overcome the technical problem of low binding amount of metallothionein and iron ions in nano # imgabs0 # prepared by a biological method in the prior art, the invention provides a method for preparing a nano metal iron oxide material from a metallothionein mutant strain, the metallothionein mutant strain is prepared by mutating metallothionein, and the metallothionein mutant strain is fermented to obtain the nano metal iron oxide material. A # imgabs 1 # nano material precursor is prepared, and the # imgabs 2 # nano material which is uniform in nano particle size and smaller in particle size is prepared through calcination. The biological preparation method of the # imgabs3 nano material provided by the invention is mild in reaction condition, environment-friendly in preparation process, green and environment-friendly, and simple and convenient to operate.

The invention belongs to the technical field of genetic engineering and microorganism application, particularly relates to a recombinant saccharomyces cerevisiae engineering bacterium capable of producing glutathione at high yield and enriching iron and zinc, and further discloses application of the recombinant saccharomyces cerevisiae engineering bacterium to preparation of an active product containing a metal chelated polyphenol compound by fermentation of pomegranate peel waste and further regulation and control of a hair follicle microenvironment to promote hair regeneration. According to the scheme, the saccharomyces cerevisiae is subjected to three-site collaborative editing (YPRC delta 15 / Ubp6 / rDNA) through a CRISPR / Cas9 technology, so that the synthesis capability of glutathione is improved, and efficient enrichment of iron and zinc is synchronously realized; the pomegranate peel waste is further utilized for fermentation, macromolecular ellagic acid ester is converted into micromolecular gallic acid, the micromolecular gallic acid and metal ions form a bioactive chelate, a Wnt / beta-catenin pathway can be activated to promote hair papilla cell proliferation, and finally a full-pathway synergistic solution of'oxidation resistance-metal homeostasis-regeneration repair 'is formed.

The present invention provides a gene combination and multi-gene expression cassette for improving cadmium accumulation and cadmium tolerance in plants, relating to the field of biological genetic engineering technology. The present invention combines the PPKS gene, the MT1ES gene, the MT2S gene, the MT3S gene, and the MT4S gene, and constructs gene expression cassettes based on the codon preference of the target plant after codon optimization. The five gene expression cassettes are then serially incorporated into a plant transformation vector to produce a recombinant plant transformation vector. The recombinant plant transformation vector is then used to transform the target plant, yielding an engineered plant capable of efficiently expressing the exogenous gene. Experimental measurements confirm that the introduction of the PPKS, MT1ES, MT2S, MT3S, and MT4S genes improves the plant's cadmium accumulation and tolerance, resulting in an engineered plant with a more ideal cadmium-contaminated soil remediation effect.

A system / agent / composition including a bacterial anti-metallothionein antibody and methods for their use to prevent and / or treat bacterial infections such as ESKAPE infections, for example, Pseudomonas aeruginosa infection are described.

The application provides a wedgedleaf chrysanthemum lanthanum-resistant gene CnMT2, an expression vector thereof, a protein and application thereof, and belongs to the technical field of genetic engineering.The nucleotide sequence of the wedgedleaf chrysanthemum lanthanum-resistant gene CnMT2 is shown as SEQ ID NO.1.It is found by the application that the CnMT2 gene is induced to express under LaCl3 stress treatment at different times, and the expression modes of the aboveground part and the underground part are different.Seed germination of transgenic Arabidopsis shows higher resistance to LaCl3 and H2O2, and the transgenic seedlings show better growth potential as the LaCl3 stress is prolonged, and the CAT and POD enzyme activities of the transgenic Arabidopsis are significantly increased.Therefore, the wedgedleaf chrysanthemum CnMT2 gene can improve the lanthanum resistance and the oxidation resistance of plants, and has the potential as an important gene resource for plant remediation.

The invention discloses a transgenic synechocystis strain with enhanced stress resistance to various heavy metal ions as well as a construction method and application of the transgenic synechocystis strain, and belongs to the technical field of microorganisms. The transgenic synechocystis algal strain is constructed by integrating a cyanobacteria high expression promoter and a gene HMP3 of a human metallothionein artificial mutant into a synechocystis PCC6803 genome. Detection shows that the transgenic synechocystis strain shows remarkably enhanced stress resistance under the stress condition of heavy metal ions such as Cr < 6 + >, Pb < 2 + > and Cd < 2 + >. Compared with a wild type algal strain, the transgenic synechocystis algal strain has higher activity of superoxide dismutase (SOD) and catalase (CAT), and the content of phycobiliprotein, chlorophyll a and carotenoid is also maintained at a higher level. The invention provides an effective method for constructing a transgenic synechocystis strain resistant to stress of various heavy metal ions, and provides a technical means for application of microalgae in heavy metal sewage treatment.