5results about "Metallothioneins" patented technology

The application discloses a method for improving the cadmium ion adsorption capacity of Escherichia coli, and belongs to the technical field of genetic engineering. E. coli BL21 to Cd 2+ , and the unit point mutation of T16C, K19C and S45C to CeMT2 can significantly improve the Cd 2+ binding capacity of CeMT2, and the cell Cd 2+ concentration of the T16C, K19C and S45C mutants is increased by 1.32 times, 1.50 times and 1.41 times, respectively. E. coli The recombinant expression of CeMT2 (K19C) and LmCadC in BL21 can obviously improve the cadmium ion adsorption capacity of Escherichia coli, and the maximum adsorption capacity reaches 8.5 mg / g.

A Pichia pastoris fermentation process and its application belong to the field of microbial fermentation technology. The technical problem to be solved is to provide an optimized Pichia pastoris fermentation process to address the shortcomings of existing technologies, such as complex purification of soluble MT, low yield, and high price. The key points of the technical solution are a Pichia pastoris fermentation process, including the following steps: (1) transferring Pichia pastoris carrying the MT gene to YPD plate medium for culture and activation to obtain the cultured strain; (2) inoculating the cultured strain obtained in step (1) into BMGY medium with an initial pH of 7-9 for shaking culture, and obtaining cell cells after centrifugation; (3) waiting for the OD of the cell cells obtained in step (2) 600 At 6-10, the medium was transferred to BMMY medium and induced metal was added to induce MT expression; (4) The process parameters were further optimized using a BP neural network model.

The invention belongs to the technical field of genetic engineering and microorganism application, particularly relates to a recombinant saccharomyces cerevisiae engineering bacterium capable of producing glutathione at high yield and enriching iron and zinc, and further discloses application of the recombinant saccharomyces cerevisiae engineering bacterium to preparation of an active product containing a metal chelated polyphenol compound by fermentation of pomegranate peel waste and further regulation and control of a hair follicle microenvironment to promote hair regeneration. According to the scheme, the saccharomyces cerevisiae is subjected to three-site collaborative editing (YPRC delta 15 / Ubp6 / rDNA) through a CRISPR / Cas9 technology, so that the synthesis capability of glutathione is improved, and efficient enrichment of iron and zinc is synchronously realized; the pomegranate peel waste is further utilized for fermentation, macromolecular ellagic acid ester is converted into micromolecular gallic acid, the micromolecular gallic acid and metal ions form a bioactive chelate, a Wnt / beta-catenin pathway can be activated to promote hair papilla cell proliferation, and finally a full-pathway synergistic solution of'oxidation resistance-metal homeostasis-regeneration repair 'is formed.

The application provides a wedgedleaf chrysanthemum lanthanum-resistant gene CnMT2, an expression vector thereof, a protein and application thereof, and belongs to the technical field of genetic engineering.The nucleotide sequence of the wedgedleaf chrysanthemum lanthanum-resistant gene CnMT2 is shown as SEQ ID NO.1.It is found by the application that the CnMT2 gene is induced to express under LaCl3 stress treatment at different times, and the expression modes of the aboveground part and the underground part are different.Seed germination of transgenic Arabidopsis shows higher resistance to LaCl3 and H2O2, and the transgenic seedlings show better growth potential as the LaCl3 stress is prolonged, and the CAT and POD enzyme activities of the transgenic Arabidopsis are significantly increased.Therefore, the wedgedleaf chrysanthemum CnMT2 gene can improve the lanthanum resistance and the oxidation resistance of plants, and has the potential as an important gene resource for plant remediation.

The invention discloses a transgenic synechocystis strain with enhanced stress resistance to various heavy metal ions as well as a construction method and application of the transgenic synechocystis strain, and belongs to the technical field of microorganisms. The transgenic synechocystis algal strain is constructed by integrating a cyanobacteria high expression promoter and a gene HMP3 of a human metallothionein artificial mutant into a synechocystis PCC6803 genome. Detection shows that the transgenic synechocystis strain shows remarkably enhanced stress resistance under the stress condition of heavy metal ions such as Cr < 6 + >, Pb < 2 + > and Cd < 2 + >. Compared with a wild type algal strain, the transgenic synechocystis algal strain has higher activity of superoxide dismutase (SOD) and catalase (CAT), and the content of phycobiliprotein, chlorophyll a and carotenoid is also maintained at a higher level. The invention provides an effective method for constructing a transgenic synechocystis strain resistant to stress of various heavy metal ions, and provides a technical means for application of microalgae in heavy metal sewage treatment.