The invention relates to an industrial method for extracting and purifying zinc metallothionin from rabbit liver. It comprises following steps: inducing, coarse extracting, chromatography purifying; inducing adult rabbit for 5- 7 times through belly hypodermic injection, the zinc injection dosage is increased by 10- 250 mg for every kg rabbit gradually; stirring rabbit liver with homogenate liquor, adding denatured liqour and stirring, freezing and centrifuging, collecting supernatant -1, heating fastly, colling fastly to normal temperature, freezing and centrifuging again, getting supernatant -2, condensing through ultra- filtering membrane, getting supernatant -3 for chromatography in gel medium, desalting collecting liquid and freeze drying. The invention is characterized by higher productivity of Zn- MT, low extracting cost, high purity, simple preparation process and convenient extracting method.