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45 results about "Plant transformation vector" patented technology
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Plant transformation vectors are plasmids that have been specifically designed to facilitate the generation of transgenic plants. The most commonly used plant transformation vectors are termed binary vectors because of their ability to replicate in both E. coli, a common lab bacterium, and Agrobacterium tumefaciens, a bacterium used to insert the recombinant (customized) DNA into plants. Plant Transformation vectors contain three key elements;
Rice pollen-preferential promoters and uses thereof
The present invention relates to rice genomic promoter sequences which promote transcription preferentially in microspores and / or pollen of plants. Also provided are chimeric genes comprising these promoter sequences, and plant transformation vectors comprising these chimeric genes. The present invention also discloses plant cells, plant tissues, plants, seeds and grains comprising these chimeric genes. The invention further discloses methods for expressing foreign nucleic acid sequences preferentially in pollen and for producing plants with modified pollen fertility.
Owner:BAYER CROPSCIENCE NV
Transgenic breeding method producing astaxanthin in crop seed endosperm
ActiveCN105907780AVector-based foreign material introductionAngiosperms/flowering plantsAgricultural scienceAstaxanthin
The invention discloses a transgenic breeding method producing astaxanthin in crop seed endosperm. The transgenic breeding method specifically includes the steps of S1, optimizing the sequence of a PSY gene, a CrtI gene, a BHY gene and a BKT gene according to the codon preference of a breeding crop, and respectively fusing the four genes with the optimized sequences with breeding crop endosperm expression promoters and transit peptides to build gene expression boxes; S2, building the four gene expression boxes obtained in the S1 into plant transformation vectors to obtain the plant transformation vectors of the PSY, the CrtI, the BHY and the BKT; S3, using the plant transformation vectors obtained in the S2 to transform the breeding crop to obtain a transgenic crop with the salmon-colored endosperm and synthesized astaxanthin. The transgenic breeding method has the advantages that the astaxanthin can be synthesized in the seed astaxanthin of crops (especially paddy rice), the crops can be directly used for eating and also be used as the raw materials for producing the astaxanthin, and the method has important guiding significance and production application value to the novel functional crop breeding.
Owner:SOUTH CHINA AGRI UNIV
Rice blast resistance gene Pi1 and application thereof
InactiveCN101906427ADoes not create linkage problems with bad genesNo cascading problemsMicrobiological testing/measurementPlant peptidesBiotechnologyDisease
The invention discloses clone and application of rice blast resistance gene Pi1. The rice blast resistance gene Pi1 comprises gene Pi1-1, or Pi1-2, or tandem sequence of the Pi1-1 and the Pi1-2, the nucleotide sequence of the Pi1-1 is shown as SEQIDNO:1, the nucleotide sequence of the Pi1-2 is shown as SEQIDNO:2, and the nucleotide sequence of the tandem sequence is shown as SEQIDNO:3. The two genes of Pi1-1 and Pi1-2 belong to members of CC-NBS-LRR resistance gene family and are constitutive expression genes. The rice blast resistance gene Pi1 can endue plants with specific disease resistance response to disease caused by rice blast fungus (Magnaporthe oryzae), and is suitable for the plants sensitive to pathogens. By connecting the Pi1 gene sequence with plants transformation carriers to be introduced to plant cells, genetically modified resistant varieties can be obtained so as to be applied to farm production.
Owner:SOUTH CHINA AGRI UNIV
A plant transformation vector and its construction method and application
InactiveCN102286524AHigh expressionOvercoming the problem of preferenceVector-based foreign material introductionAngiosperms/flowering plantsChloroplastMethyltransferase
The invention provides a plant transformation vector, and a construction method and application thereof. In the vector, a red fluorescent protein UPMT (uroporphyrinogen III methyltransferase) and a resistance screening alad gene are used as a reporter gene and a selected marker for plant transfection. The red fluorescent protein UPMT and the resistance screening alad gene have similar functions, are superior to antibiotic and a green fluorescent protein fusion marker for example, and can be used for screening and subcellular organelle positioning in rice chloroplast transformation. Thus, the two genes used as screening markers of a transgenic plant can indicate the positioning of the cytologic and tissue specificity of a target gene, and can also promote the photosynthesis efficiency of plants and the accumulation of nutrient substances. The invention has potential and broad application backgrounds in the transgenic plant research and marketing process, and is hopefully developed into a dominant selected marker system in plant transformation.
Owner:ANHUI AGRICULTURAL UNIVERSITY
Methods for targeted transgene-integration using custom site-specific DNA recombinases
The invention relates to biotechnology and provides novel methods for sequence-specific or sequence-directed transcription activator-like effector recombinase-mediated integration of DNA sequences of interest into host genomes. The invention also provides methods of use for novel plant transformation vectors and expression cassettes, which include novel combinations of chimeric recombinases with plant expression and transformation elements. Methods for gene-targeting, DNA sequence removal, genome modification, and molecular breeding are also provided.
Owner:MONSANTO TECH LLC
Production of Plants with Decreased Nitrite Content
InactiveUS20110239324A1Reduce contentReduce nitriteSugar derivativesOther foreign material introduction processesPlant cellNitrite reductase
The present invention relates in one aspect to a method for producing a transgenic plant, comprising introducing into an unmodified plant an exogenous gene encoding a nitrite reductase, wherein expression of the nitrite reductase encoded by the exogenous gene reduces nitrite content in the transgenic plant relative to the unmodified plant. Also provided are transgenic plants and plant cells comprising an exogenous gene encoding a nitrite reductase, as well as associated uses, chimaeric genes and plant transformation vectors.
Owner:BRITISH AMERICAN TOBACCO (INVESTMENTS) LTD
Production of plants with decreased nitrite content
ActiveUS20150203858A1Reduce contentReduce nitriteSugar derivativesMutant preparationNitrite reductasePlant cell
The present invention relates in one aspect to a method for producing a transgenic plant, comprising introducing into an unmodified plant an exogenous gene encoding a nitrite reductase, wherein expression of the nitrite reductase encoded by the exogenous gene reduces nitrite content in the transgenic plant relative to the unmodified plant. Also provided are transgenic plants and plant cells comprising an exogenous gene encoding a nitrite reductase, as well as associated uses, chimaeric genes and plant transformation vectors.
Owner:BRITISH AMERICAN TOBACCO (INVESTMENTS) LTD
Method for creating beet salt-resisting and anti-draught new seeds using stem-tip growth-point genetic conversion
InactiveCN1404720AIncrease multiplierHigh-quality acceptor materialPlant genotype modificationShoot apexGermplasm
The method for breeding salt-resistant drought-resistant new variety of sugar beet by utilizing shoot tip growing point genetic transformation includes the following main steps: using shoot tip growing point or tufted bud growing point of the sugar beet as receptor, using agrobacterium containing plant transformation carrier system to infect beet cell to produce transgenic plant, detecting exogenous gene and its expression strength and adverse-resistance of plant of setect out the salt-resistant drought-resistance transgenic plant. Said system uses the teneral inflorescence section of alabastrum stage of beet as explant, includes the tufted bud to produce and make mass proliferation on proper culture medium, strips foliode and petiolute to denude growing point for agrobacterium infection.
Owner:SHANDONG UNIV
Garland-flower sesquiterpene synthetase gene HcTPS12 and application thereof
ActiveCN109797161AHigh content of ingredientsIncrease resistanceMicrobiological testing/measurementFermentationComplementary deoxyribonucleic acidRhizome
The invention discloses a garland-flower sesquiterpene synthetase gene HcTPS12 and an application thereof. The full-length cDNA (complementary deoxyribonucleic acid) sequence of the gene HcTPS12 is asshown in SEQ ID NO: 1, the coding sequence of the gene is as shown in SEQ ID NO: 2, and the coded amino acid sequence of the gene is as shown in SEQ ID NO: 3. The expression quantity of the gene HcTPS12 in garland-flower leaf tissues is high, the gene is hardly expressed in organs such as rhizomes and flowers, and the expression quantity is regulated by leaf development. After a heterologous recombinant protein substrate of the gene HcTPS12 is catalyzed, bisabolene serving as a sesquiterpene medicinal component can be generated, and the gene can be used for preparing the bisabolene and further preparing essential oil, essence and medicines. The gene HcTPS12 is connected with a plant transformation vector and then led into cells of a garland-flower or other plants, and a transgenic plant expressing the gene can be acquired. Besides, a specific molecular marker is generated according to gene sequence information and used for identifying the sesquiterpene bisabolene synthetase gene of the garland-flower or other plants and molecular marker assistant selection, so that selection efficiency is improved, and the gene has a large application prospect.
Owner:SOUTH CHINA AGRI UNIV
Rice mitochondria sterility gene and application thereof
The invention relates to the technical field of rice breeding, and specifically relates to a rice mitochondria sterility gene and the application thereof. The invention discloses a rice mitochondria sterility gene and a coding sequence thereof, and D1 type cytoplasm can be quickly and efficiently screened and authenticated from wild rice for cultivation of a D1 type cytoplasm sterile line by designing a specific molecular marker through the gene sequence. A mitochondrial genome specific sequence of the D1 type cytoplasm sterile is authenticated through comparative genomics, sterility related ORFs are authenticated through gene predication and differential expression analysis, and a plant transformation vecter is constructed for transforming maintenance line verification and sterility function.
Owner:JIANGXI SUPER RICE RES & DEV CENT (HAINAN RICE BREEDING CENT OF JIANGXI ACAD OF AGRI SCI) +1
Plant repair method of applying transgenetic plant composite system to polluted soil
ActiveCN110283845AStrong resistanceLarge biomassContaminated soil reclamationGenetic engineeringGenetically engineeredCadmium Cation
The invention discloses a plant repair method of applying a transgenetic plant composite system to polluted soil. A transgenetic plant is obtained by steps of construction of a plant transformation vector, plant genetic transformation and the like. The transgenetic plant can be used for plant repair of heavy metal polluted soil and water bodies. The transgenetic plant shows a hyperaccumulating characteristic and resistance to different heavy metals such as lead and cadmium, can be widely applied to repairing heavy metal polluted soil, and has efficient repair efficiency to the polluted soil. The plant repair method can be used to polluted soil caused by mining, soil pollution of surrounding earth piles, and polluted soil of a waste site of an electroplate factory and the periphery, for example, various composite metal polluted soil such as Cd, Cr, Cu, Pb, Z and Ni. The plant repair method has the multiple advantages of being high in adaptability, environmentally friendly and the like in repair of composite polluted soil, and has a wide application prospect.
Owner:INST OF GEOGRAPHICAL SCI & NATURAL RESOURCE RES CAS
Ginger flower floral specific and damage and pest induced TPS2 (Trehalose-6-Phosphate Synthase 2) promoter and application thereof
ActiveCN103525816ADoes not create linkage problems with bad genesShorten breeding timeFungiBacteriaNucleotideGenotype
The invention belongs to the technical field of gene engineering and particularly discloses a ginger flower floral specific and damage and pest induced TPS2 (Trehalose-6-Phosphate Synthase 2) promoter and an application thereof. The promoter has a nucleotide sequence as shown in SEQ ID NO: 2. A target gene controlled by the TPS2 promoter disclosed by the invention is only expressed in a flower tissue of a transgenic tobacco, and the expression is relevant to a flower development process and is induced by damage and pests. In addition, the TPS2 promoter disclosed by the invention can be introduced to a cell of a ginger flower or other plants after being connected to any one plant transformation vector to obtain promoter-containing transgenic fragrance and resistance to be used for production; or a specific molecular marker can also be generated according to the sequence information of the promoter disclosed by the invention and is used for authenticating the fragrance and resistance genotypes of the ginger flower or other plants and realizing the molecular-marker-assisted selective breeding, so that the breeding selection efficiency is increased.
Owner:SOUTH CHINA AGRI UNIV
Lily ester floral gene LoAAT1 and application thereof
ActiveCN103436537ANo cascading problemsContribute to the generationFungiBacteriaNucleotidePlant cell
The invention belongs to the technical field of gene engineering, and in particular discloses a lily ester floral gene LoAAT1 and application thereof. A nucleotide sequence of the LoAAT1 gene is shown in SEQ ID NO: 1-2, and an amino acid sequence of protein coded by the gene is shown in SEQ ID NO: 3. The LoAAT1 gene is expressed in lily tissue with fragrance and is not expressed in lily varieties having no fragrance, and the expression s related to a development process of flower. The LoAAT1 gene can be linked to any one plant transformation vector and introduced into cells of lily or other plants, to obtain transgenic fragrance expressing the gene, thus being used for production; in addition, a molecular marker can be generated depending on the gene sequence information, and the molecular marker is used for identifying fragrance genotype of lily or other plants and for marker-assisted selective breeding, thus enhancing selecting efficiency of breeding.
Owner:SOUTH CHINA AGRI UNIV
Ginger flower floral specific and damage and pest induced TPS2 (Trehalose-6-Phosphate Synthase 2) promoter and application thereof
ActiveCN103525816BDoes not create linkage problems with bad genesShorten breeding timeFungiBacteriaTrehalose-6-phosphate synthaseNucleotide
The invention belongs to the technical field of gene engineering and particularly discloses a ginger flower floral specific and damage and pest induced TPS2 (Trehalose-6-Phosphate Synthase 2) promoter and an application thereof. The promoter has a nucleotide sequence as shown in SEQ ID NO: 2. A target gene controlled by the TPS2 promoter disclosed by the invention is only expressed in a flower tissue of a transgenic tobacco, and the expression is relevant to a flower development process and is induced by damage and pests. In addition, the TPS2 promoter disclosed by the invention can be introduced to a cell of a ginger flower or other plants after being connected to any one plant transformation vector to obtain promoter-containing transgenic fragrance and resistance to be used for production; or a specific molecular marker can also be generated according to the sequence information of the promoter disclosed by the invention and is used for authenticating the fragrance and resistance genotypes of the ginger flower or other plants and realizing the molecular-marker-assisted selective breeding, so that the breeding selection efficiency is increased.
Owner:SOUTH CHINA AGRI UNIV
Lily ester floral gene LoAAT1 and application thereof
ActiveCN103436537BNo cascading problemsContribute to the generationFungiBacteriaNucleotidePlant cell
The invention belongs to the technical field of gene engineering, and in particular discloses a lily ester floral gene LoAAT1 and application thereof. A nucleotide sequence of the LoAAT1 gene is shown in SEQ ID NO: 1-2, and an amino acid sequence of protein coded by the gene is shown in SEQ ID NO: 3. The LoAAT1 gene is expressed in lily tissue with fragrance and is not expressed in lily varieties having no fragrance, and the expression s related to a development process of flower. The LoAAT1 gene can be linked to any one plant transformation vector and introduced into cells of lily or other plants, to obtain transgenic fragrance expressing the gene, thus being used for production; in addition, a molecular marker can be generated depending on the gene sequence information, and the molecular marker is used for identifying fragrance genotype of lily or other plants and for marker-assisted selective breeding, thus enhancing selecting efficiency of breeding.
Owner:SOUTH CHINA AGRI UNIV
Multi-gene plant expression vector and construction method as well as application thereof
InactiveCN102703498AAvoid self connectionEasy to operateVector-based foreign material introductionAngiosperms/flowering plantsPhosphorylationTail
The invention discloses a multi-gene plant expression vector and a construction method as well as application thereof. The multi-gene plant expression vector comprises a plant transformation vector p209, a cloning vector p6435x and one or more target genes to be transformed. According to construction of the multi-gene plant expression vector, by utilizing the characteristic that the original enzyme cutting site disappears after isocaudarners are connected, and the two ends of a vector or a segment after enzyme cutting are different viscous segments all the time; and thus, the problem of limited endonuclease sites of the conventional vector is solved, meanwhile, self-connection in a vector connection process is also avoided, phosphorylation treatment at the viscous tail end is not required, an intermediate vector and a special engineering strain are not required yet, most of restriction endonuclease sites are avoided, the conventional steps of enzyme cutting, connection, transformation and the like are not required, the operation steps are simplified, the experimental difficulty is reduced, and the technology is mature and reliable, and low in cost, can be finished under normal experimental conditions and is convenient to promote.
Owner:HEBEI AGRICULTURAL UNIV.
Improvement of rape pod shatter resistance by BnaA06FUL1 gene
The invention belongs to the technical field of plant genetic engineering, and in particular, relates to improvement of rape pod shatter resistance by a BnaA06FUL1 gene. The tissue expression of the BnaA06FUL1 gene in brassica napus and differential expression conditions of pod shatter-resistant and easy-to-pod shattered materials are analyzed. The brassica napus is transformed by construction ofa plant transformation vector and overexpressing of the BnaA06FUL1 gene, the pod shatter resistance of the brassica napus is improved, but other characters are not affected. The nucleotide sequence ofthe cloned BnaA06.FUL1 is shown in SEQ:ID NO:1. With brassica napus as a receptor, a transgenic plant overexpressing the brassica napus BnaA06FUL1 gene and having the pod shatter resistance characteris obtained by the agrobacterium-mediated transformation method. The BnaA06FUL1 gene can be used for improving pod shatter resistance ability of crops, especially brassica napus.
Owner:INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
Method for constructing a universal plant expression vector
InactiveCN102978237AImprove build success rateOvercome the defect of substantial drop in efficiencyPlant tissue cultureHorticulture methodsGenetically modified riceCell restriction
The invention provides a method for constructing a universal plant expression vector, and belongs to the technical field of organism. The method comprises the steps of constructing an intermediate vector containing a promoter terminator; connecting a target gene to an efficient plant transformation vector pCMBIA1300 by the intermediate vector to obtain the plant expression vector; transforming the constructed plant transformation vector into a rice cell, screening the transformed regeneration plant (T0 generation) from explants in a plant regeneration medium, and detecting a transformation system to obtain the transgenic rice. The method disclosed by the invention can construct the plant expression vector by double polyclonal restriction points of the vector, so as to expand the application range of the method.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
Ethylene insensitive plants
Nucleic acid and polypeptide sequences are described which relate to an EIN6 gene, a gene involved in the plant ethylene response. Plant transformation vectors and transgenic plants are described which display an altered ethylene-dependent phenotype due to altered expression of EIN6 in transformed plants.
Owner:SALK INST FOR BIOLOGICAL STUDIES +1
Decreasing nitrite content in tobacco via expression of a nitrite reductase
Owner:BRITISH AMERICAN TOBACCO (INVESMENTS) LTD
Promoters functional in plant plastids
The present invention relates to isolated nucleic acid molecule comprising a nucleic acid promoter or untranslated region comprising the nucleic acid sequence of SEQ ID NO: 1, 2 or 3. The invention also relates to isolated nucleic acid promoter or untranslated region comprising the nucleic acid sequence of SEQ ID NO: 1, 2 or 3. The invention further relates to chimeric genes comprising the isolated nucleic acid promoter or untranslated region of SEQ ID NO:1, 2 or 3 operatively linked to the coding sequence of a gene of interest. The invention also relates to plant transformation vectors comprising the chimeric genes of the invention. The invention further relates to transgenic plants, plant cells, plant seeds, plant tissues, or plant plastids, comprising the chimeric genes of this invention.
Owner:SYNGENTA PARTICIPATIONS AG
A phytoremediation method of a transgenic plant complex system applied to polluted soil
ActiveCN110283845BStrong resistanceLarge biomassContaminated soil reclamationFermentationSoil sciencePollution soil
Owner:INST OF GEOGRAPHICAL SCI & NATURAL RESOURCE RES CAS
Rice mitochondrial sterile gene and application thereof
ActiveUS10837068B2Rapidly and effectively screen and identifyMicrobiological testing/measurementPlant peptidesBiotechnologyOryza rufipogon
Provided is a rice mitochondrial sterile gene and the application thereof. A rice mitochondrial sterile gene and a coding sequence thereof are disclosed. A specific molecular marker was designed from said gene sequence and enables a rapid and effective screening and identification of D1-type cytoplasm from wild rice for breeding a new D1-type cytoplasm sterile line. A specific sequence in the mitochondria genome of D1-type cytoplasm sterile line was identified by comparative genomics. Sterility-related ORFs were identified from gene prediction and expression difference analysis. A plant transformation vector was constructed to transform maintainer line for verification and sterile function.
Owner:JIANGXI SUPER RICE RES & DEV CENT +1
Methods for targeted transgene-integration using custom site-specific DNA recombinases
The invention relates to biotechnology and provides novel methods for sequence-specific or sequence-directed transcription activator-like effector recombinase-mediated integration of DNA sequences of interest into host genomes. The invention also provides methods of use for novel plant transformation vectors and expression cassettes, which include novel combinations of chimeric recombinases with plant expression and transformation elements. Methods for gene-targeting, DNA sequence removal, genome modification, and molecular breeding are also provided.
Owner:MONSANTO TECH LLC
Transgenic Plant for Detecting Environmental Chemicals
InactiveUS20080318335A1Not much loadThe process is simple and fastAnalysis by subjecting material to chemical reactionBiological testingTransgeneGMO Plants
The present invention provides a plant transformation vector for producing a plant for detecting an environmental chemical by a change of flower color, the plant transformation vector comprising an AhR gene expressibly incorporated therein, an AhR ligand stimulus-inducible promoter, and an expression suppressive factor for suppressing the expression of a gene involved in the synthesis of a desired flower pigment, the factor being expressibly incorporated therein by the promoter.
Owner:SUNTORY HLDG LTD
Promoter for the high level expression in plant-tissue culture and vector using the same
ActiveUS20100100986A1High activityHigh level expressionBryophytesSugar derivativesSuspension culturePlant transformation vector
Disclosed herein are a promoter (SEQ ID NO.: 1) inducing high level expression of a target gene in plant tissue cultured cells, derived from the sweetpotato gene of Ran GTPase, small GTP binding protein, a plant transformation vector for carrying the same and a method for expressing a foreign gene in plant cell using the vector. The activity of promoter according to the present invention is higher than that of universal CaMV 35S promoter in transgenic suspension cultured cell lines, calluses and adventitious roots. Thus, the promoter is useful in the generation of transgenic cell lines including cultured roots to produce valuable materials such as medicinal or industrial proteins in a large quantities with plant tissue cultured cells.
Owner:BIO FD&C CO LTD
A kind of Agrobacterium transformation vector composition for obtaining marker-free transgenic plants and its application
Owner:INST OF CROP SCI CHINESE ACAD OF AGRI SCI
Promoter from sweet potato ran GTPase gene for the high level expression in plant-tissue culture and vector using the same
ActiveUS8232453B2High activityHigh expressionBryophytesSugar derivativesSmall GTP binding proteinPlant tissue
Disclosed herein are a promoter (SEQ ID NO.: 1) inducing high level expression of a target gene in plant tissue cultured cells, derived from the sweetpotato gene of Ran GTPase, small GTP binding protein, a plant transformation vector for carrying the same and a method for expressing a foreign gene in plant cell using the vector. The activity of promoter according to the present invention is higher than that of universal CaMV 35S promoter in transgenic suspension cultured cell lines, calluses and adventitious roots. Thus, the promoter is useful in the generation of transgenic cell lines including cultured roots to produce valuable materials such as medicinal or industrial proteins in a large quantities with plant tissue cultured cells.
Owner:BIO FD&C CO LTD
Plant transformation vector, and construction method and application thereof
InactiveCN102286524BHigh expressionOvercoming the problem of preferenceVector-based foreign material introductionAngiosperms/flowering plantsBiotechnologyChloroplast
The invention provides a plant transformation vector, and a construction method and application thereof. In the vector, a red fluorescent protein UPMT (uroporphyrinogen III methyltransferase) and a resistance screening alad gene are used as a reporter gene and a selected marker for plant transfection. The red fluorescent protein UPMT and the resistance screening alad gene have similar functions, are superior to antibiotic and a green fluorescent protein fusion marker for example, and can be used for screening and subcellular organelle positioning in rice chloroplast transformation. Thus, the two genes used as screening markers of a transgenic plant can indicate the positioning of the cytologic and tissue specificity of a target gene, and can also promote the photosynthesis efficiency of plants and the accumulation of nutrient substances. The invention has potential and broad application backgrounds in the transgenic plant research and marketing process, and is hopefully developed into a dominant selected marker system in plant transformation.
Owner:ANHUI AGRICULTURAL UNIVERSITY
A kind of ginger flower sesquiterpene synthase gene hctps14 and its application
ActiveCN109722443BHigh content of ingredientsIncrease resistanceMicrobiological testing/measurementFermentationBiotechnologyPlant cell
The invention discloses a gingerflower sesquiterpene synthase gene HcTPS14 and its application. The full-length cDNA sequence of the HcTPS14 gene is shown in SEQ ID NO:1; the coding sequence is shown in SEQ ID NO:2; the encoded amino acid sequence is shown in SEQ ID NO:3. The HcTPS14 gene is expressed to a certain extent in leaf tissue and rhizome, but hardly expressed in flower organs, and its expression level is regulated by leaf development. The exogenous recombinant protein of HcTPS14, after catalyzing the substrate, can generate α-caryophyllene, a medicinal ingredient of sesquiterpene, which can be used to prepare α-caryophyllene and further prepare essential oils, essences and drugs; connect HcTPS14 to the plant transformation vector, and then introduce In Ginger Flower or other plant cells, transgenic plants expressing the gene can be obtained; specific molecular markers can also be produced according to the gene sequence information of the present invention, for identifying the sesquiterpene α-Caryophyllum of Ginger Flower or other plants The alkene synthase gene is used for molecular marker-assisted selection breeding, thereby improving the selection efficiency of breeding, and has great application prospects.
Owner:SOUTH CHINA AGRI UNIV
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