43 results about "Plant transformation vector" patented technology

The invention discloses a transgenic breeding method producing astaxanthin in crop seed endosperm. The transgenic breeding method specifically includes the steps of S1, optimizing the sequence of a PSY gene, a CrtI gene, a BHY gene and a BKT gene according to the codon preference of a breeding crop, and respectively fusing the four genes with the optimized sequences with breeding crop endosperm expression promoters and transit peptides to build gene expression boxes; S2, building the four gene expression boxes obtained in the S1 into plant transformation vectors to obtain the plant transformation vectors of the PSY, the CrtI, the BHY and the BKT; S3, using the plant transformation vectors obtained in the S2 to transform the breeding crop to obtain a transgenic crop with the salmon-colored endosperm and synthesized astaxanthin. The transgenic breeding method has the advantages that the astaxanthin can be synthesized in the seed astaxanthin of crops (especially paddy rice), the crops can be directly used for eating and also be used as the raw materials for producing the astaxanthin, and the method has important guiding significance and production application value to the novel functional crop breeding.

The invention relates to biotechnology and provides novel methods for sequence-specific or sequence-directed transcription activator-like effector recombinase-mediated integration of DNA sequences of interest into host genomes. The invention also provides methods of use for novel plant transformation vectors and expression cassettes, which include novel combinations of chimeric recombinases with plant expression and transformation elements. Methods for gene-targeting, DNA sequence removal, genome modification, and molecular breeding are also provided.

The present invention relates in one aspect to a method for producing a transgenic plant, comprising introducing into an unmodified plant an exogenous gene encoding a nitrite reductase, wherein expression of the nitrite reductase encoded by the exogenous gene reduces nitrite content in the transgenic plant relative to the unmodified plant. Also provided are transgenic plants and plant cells comprising an exogenous gene encoding a nitrite reductase, as well as associated uses, chimaeric genes and plant transformation vectors.

The present invention relates in one aspect to a method for producing a transgenic plant, comprising introducing into an unmodified plant an exogenous gene encoding a nitrite reductase, wherein expression of the nitrite reductase encoded by the exogenous gene reduces nitrite content in the transgenic plant relative to the unmodified plant. Also provided are transgenic plants and plant cells comprising an exogenous gene encoding a nitrite reductase, as well as associated uses, chimaeric genes and plant transformation vectors.

The method for breeding salt-resistant drought-resistant new variety of sugar beet by utilizing shoot tip growing point genetic transformation includes the following main steps: using shoot tip growing point or tufted bud growing point of the sugar beet as receptor, using agrobacterium containing plant transformation carrier system to infect beet cell to produce transgenic plant, detecting exogenous gene and its expression strength and adverse-resistance of plant of setect out the salt-resistant drought-resistance transgenic plant. Said system uses the teneral inflorescence section of alabastrum stage of beet as explant, includes the tufted bud to produce and make mass proliferation on proper culture medium, strips foliode and petiolute to denude growing point for agrobacterium infection.

The invention discloses a plant repair method of applying a transgenetic plant composite system to polluted soil. A transgenetic plant is obtained by steps of construction of a plant transformation vector, plant genetic transformation and the like. The transgenetic plant can be used for plant repair of heavy metal polluted soil and water bodies. The transgenetic plant shows a hyperaccumulating characteristic and resistance to different heavy metals such as lead and cadmium, can be widely applied to repairing heavy metal polluted soil, and has efficient repair efficiency to the polluted soil. The plant repair method can be used to polluted soil caused by mining, soil pollution of surrounding earth piles, and polluted soil of a waste site of an electroplate factory and the periphery, for example, various composite metal polluted soil such as Cd, Cr, Cu, Pb, Z and Ni. The plant repair method has the multiple advantages of being high in adaptability, environmentally friendly and the like in repair of composite polluted soil, and has a wide application prospect.

The invention belongs to the technical field of gene engineering and particularly discloses a ginger flower floral specific and damage and pest induced TPS2 (Trehalose-6-Phosphate Synthase 2) promoter and an application thereof. The promoter has a nucleotide sequence as shown in SEQ ID NO: 2. A target gene controlled by the TPS2 promoter disclosed by the invention is only expressed in a flower tissue of a transgenic tobacco, and the expression is relevant to a flower development process and is induced by damage and pests. In addition, the TPS2 promoter disclosed by the invention can be introduced to a cell of a ginger flower or other plants after being connected to any one plant transformation vector to obtain promoter-containing transgenic fragrance and resistance to be used for production; or a specific molecular marker can also be generated according to the sequence information of the promoter disclosed by the invention and is used for authenticating the fragrance and resistance genotypes of the ginger flower or other plants and realizing the molecular-marker-assisted selective breeding, so that the breeding selection efficiency is increased.

The invention belongs to the technical field of gene engineering, and in particular discloses a lily ester floral gene LoAAT1 and application thereof. A nucleotide sequence of the LoAAT1 gene is shown in SEQ ID NO: 1-2, and an amino acid sequence of protein coded by the gene is shown in SEQ ID NO: 3. The LoAAT1 gene is expressed in lily tissue with fragrance and is not expressed in lily varieties having no fragrance, and the expression s related to a development process of flower. The LoAAT1 gene can be linked to any one plant transformation vector and introduced into cells of lily or other plants, to obtain transgenic fragrance expressing the gene, thus being used for production; in addition, a molecular marker can be generated depending on the gene sequence information, and the molecular marker is used for identifying fragrance genotype of lily or other plants and for marker-assisted selective breeding, thus enhancing selecting efficiency of breeding.

The invention discloses a multi-gene plant expression vector and a construction method as well as application thereof. The multi-gene plant expression vector comprises a plant transformation vector p209, a cloning vector p6435x and one or more target genes to be transformed. According to construction of the multi-gene plant expression vector, by utilizing the characteristic that the original enzyme cutting site disappears after isocaudarners are connected, and the two ends of a vector or a segment after enzyme cutting are different viscous segments all the time; and thus, the problem of limited endonuclease sites of the conventional vector is solved, meanwhile, self-connection in a vector connection process is also avoided, phosphorylation treatment at the viscous tail end is not required, an intermediate vector and a special engineering strain are not required yet, most of restriction endonuclease sites are avoided, the conventional steps of enzyme cutting, connection, transformation and the like are not required, the operation steps are simplified, the experimental difficulty is reduced, and the technology is mature and reliable, and low in cost, can be finished under normal experimental conditions and is convenient to promote.

The invention belongs to the technical field of plant genetic engineering, and in particular, relates to improvement of rape pod shatter resistance by a BnaA06FUL1 gene. The tissue expression of the BnaA06FUL1 gene in brassica napus and differential expression conditions of pod shatter-resistant and easy-to-pod shattered materials are analyzed. The brassica napus is transformed by construction ofa plant transformation vector and overexpressing of the BnaA06FUL1 gene, the pod shatter resistance of the brassica napus is improved, but other characters are not affected. The nucleotide sequence ofthe cloned BnaA06.FUL1 is shown in SEQ:ID NO:1. With brassica napus as a receptor, a transgenic plant overexpressing the brassica napus BnaA06FUL1 gene and having the pod shatter resistance characteris obtained by the agrobacterium-mediated transformation method. The BnaA06FUL1 gene can be used for improving pod shatter resistance ability of crops, especially brassica napus.

The invention provides a method for constructing a universal plant expression vector, and belongs to the technical field of organism. The method comprises the steps of constructing an intermediate vector containing a promoter terminator; connecting a target gene to an efficient plant transformation vector pCMBIA1300 by the intermediate vector to obtain the plant expression vector; transforming the constructed plant transformation vector into a rice cell, screening the transformed regeneration plant (T0 generation) from explants in a plant regeneration medium, and detecting a transformation system to obtain the transgenic rice. The method disclosed by the invention can construct the plant expression vector by double polyclonal restriction points of the vector, so as to expand the application range of the method.

The present invention provides a plant transformation vector for producing a plant for detecting an environmental chemical by a change of flower color, the plant transformation vector comprising an AhR gene expressibly incorporated therein, an AhR ligand stimulus-inducible promoter, and an expression suppressive factor for suppressing the expression of a gene involved in the synthesis of a desired flower pigment, the factor being expressibly incorporated therein by the promoter.

Disclosed herein are a promoter (SEQ ID NO.: 1) inducing high level expression of a target gene in plant tissue cultured cells, derived from the sweetpotato gene of Ran GTPase, small GTP binding protein, a plant transformation vector for carrying the same and a method for expressing a foreign gene in plant cell using the vector. The activity of promoter according to the present invention is higher than that of universal CaMV 35S promoter in transgenic suspension cultured cell lines, calluses and adventitious roots. Thus, the promoter is useful in the generation of transgenic cell lines including cultured roots to produce valuable materials such as medicinal or industrial proteins in a large quantities with plant tissue cultured cells.

The invention discloses a hedychium coronarium sesquiterpene synthetase gene HcTPS14 and application thereof. The full-length cDNA sequence of the HcTPS14 gene is shown in SEQ ID NO: 1; the coding sequence is shown in SEQ ID NO:2; and the coded amino acid sequence is shown in SEQ ID NO: 3. The HcTPS14 gene has certain expression in both leaf tissues and rhizomes; however, the HcTPS14 gene is hardly expressed in floral organs. Moreover, the expression level of the HcTPS14 gene is regulated by leaf development. Being used for catalyzing substrates, exogenous recombinant proteins with the HcTPS14gene allow generation of a medicinal sesquiterpene component, namely alpha-caryophyllene; and thus, the HcTPS14 gene can be used for preparing alpha-caryophyllene, and further into essential oil, essence and pharmaceuticals. Being connected with plant transformation vectors, and then introduced into cells of hedychium coronarium or other plants, the HcTPS14 gene allows obtaining of transgenic plants expressing the HcTPS14 gene. Moreover, specific molecular markers can be produced on basis of the gene sequence information disclosed by the invention so as to be used for identifying sesquiterpene-alpha-caryophyllene synthetase genes of hedychium coronarium and other plants, thereby realizing molecular-marker-assisted selected breeding so as to improve selection efficiency of breeding. Thus,relatively great application prospects are ensured.