45 results about "Plant transformation vector" patented technology

The present invention relates to rice genomic promoter sequences which promote transcription preferentially in microspores and / or pollen of plants. Also provided are chimeric genes comprising these promoter sequences, and plant transformation vectors comprising these chimeric genes. The present invention also discloses plant cells, plant tissues, plants, seeds and grains comprising these chimeric genes. The invention further discloses methods for expressing foreign nucleic acid sequences preferentially in pollen and for producing plants with modified pollen fertility.

The invention belongs to the technical field of gene engineering and particularly discloses a ginger flower floral specific and damage and pest induced TPS2 (Trehalose-6-Phosphate Synthase 2) promoter and an application thereof. The promoter has a nucleotide sequence as shown in SEQ ID NO: 2. A target gene controlled by the TPS2 promoter disclosed by the invention is only expressed in a flower tissue of a transgenic tobacco, and the expression is relevant to a flower development process and is induced by damage and pests. In addition, the TPS2 promoter disclosed by the invention can be introduced to a cell of a ginger flower or other plants after being connected to any one plant transformation vector to obtain promoter-containing transgenic fragrance and resistance to be used for production; or a specific molecular marker can also be generated according to the sequence information of the promoter disclosed by the invention and is used for authenticating the fragrance and resistance genotypes of the ginger flower or other plants and realizing the molecular-marker-assisted selective breeding, so that the breeding selection efficiency is increased.

The invention belongs to the technical field of gene engineering, and in particular discloses a lily ester floral gene LoAAT1 and application thereof. A nucleotide sequence of the LoAAT1 gene is shown in SEQ ID NO: 1-2, and an amino acid sequence of protein coded by the gene is shown in SEQ ID NO: 3. The LoAAT1 gene is expressed in lily tissue with fragrance and is not expressed in lily varieties having no fragrance, and the expression s related to a development process of flower. The LoAAT1 gene can be linked to any one plant transformation vector and introduced into cells of lily or other plants, to obtain transgenic fragrance expressing the gene, thus being used for production; in addition, a molecular marker can be generated depending on the gene sequence information, and the molecular marker is used for identifying fragrance genotype of lily or other plants and for marker-assisted selective breeding, thus enhancing selecting efficiency of breeding.

The present invention relates in one aspect to a method for producing a transgenic plant, comprising introducing into an unmodified plant an exogenous gene encoding a nitrite reductase, wherein expression of the nitrite reductase encoded by the exogenous gene reduces nitrite content in the transgenic plant relative to the unmodified plant. Also provided are transgenic plants and plant cells comprising an exogenous gene encoding a nitrite reductase, as well as associated uses, chimaeric genes and plant transformation vectors.

The invention discloses a transgenic plant and its application in phytoremediation of polluted soil. Transgenic plants are obtained through steps such as the construction of plant transformation vectors and plant genetic transformation. Transgenic plants can be used for phytoremediation of soil and water bodies polluted by heavy metals. The transgenic plant of the invention exhibits super-accumulation characteristics and resistance to different heavy metals such as lead and cadmium, can be widely used in phytoremediation of soil polluted by heavy metals, and has high remediation efficiency for polluted soil. The invention can pollute the polluted soil caused by mining and the soil of the surrounding soil dumps; the abandoned site of the electroplating factory and the polluted soil around, such as the polluted soil of various composite metals such as Cd, Cr, Cu, Pb, Zn and Ni. The invention has multiple advantages such as strong adaptability and environmental friendliness in remediating compound polluted soil, and has wide application prospects.

Provided is a rice mitochondrial sterile gene and the application thereof. A rice mitochondrial sterile gene and a coding sequence thereof are disclosed. A specific molecular marker was designed from said gene sequence and enables a rapid and effective screening and identification of D1-type cytoplasm from wild rice for breeding a new D1-type cytoplasm sterile line. A specific sequence in the mitochondria genome of D1-type cytoplasm sterile line was identified by comparative genomics. Sterility-related ORFs were identified from gene prediction and expression difference analysis. A plant transformation vector was constructed to transform maintainer line for verification and sterile function.

The invention discloses an agrobacterium-mediated transformation vector composition for obtaining marker-free transgenic plants and application of the agrobacterium-mediated transformation vector composition, and provides a special agrobacterium vector composition for obtaining marker-free transgenic plants. The special vector composition is composed of an individually-packed recombinant vector and the pClean-Soup vector of a pClean series vector; a target gene is inserted into the T-DNA region of the pClean-Green vector of the pClean seriesvector and a marker gene is inserted into the other T-DNA region of the pClean-Green vector to obtain the recombinant vector with the expression of the target gene and the marker gene. As can be seen, with the adoption of the 5TBTG154 composition of the target gene and the marker gene in different T-DNA regions of the same vector, marker-free transgenic plants in a higher proportion can be obtained from progenies. The agrobacterium-mediated transformation vector composition for obtaining the marker-free transgenic plants and the application of the agrobacterium-mediated transformation vector composition have great significance on the transgenic plant research, especially on acquisition and utilization of wheat marker-free transgenic plants.

The invention provides a plant transformation vector, and a construction method and application thereof. In the vector, a red fluorescent protein UPMT (uroporphyrinogen III methyltransferase) and a resistance screening alad gene are used as a reporter gene and a selected marker for plant transfection. The red fluorescent protein UPMT and the resistance screening alad gene have similar functions, are superior to antibiotic and a green fluorescent protein fusion marker for example, and can be used for screening and subcellular organelle positioning in rice chloroplast transformation. Thus, the two genes used as screening markers of a transgenic plant can indicate the positioning of the cytologic and tissue specificity of a target gene, and can also promote the photosynthesis efficiency of plants and the accumulation of nutrient substances. The invention has potential and broad application backgrounds in the transgenic plant research and marketing process, and is hopefully developed into a dominant selected marker system in plant transformation.

The invention discloses a gingerflower sesquiterpene synthase gene HcTPS14 and its application. The full-length cDNA sequence of the HcTPS14 gene is shown in SEQ ID NO:1; the coding sequence is shown in SEQ ID NO:2; the encoded amino acid sequence is shown in SEQ ID NO:3. The HcTPS14 gene is expressed to a certain extent in leaf tissue and rhizome, but hardly expressed in flower organs, and its expression level is regulated by leaf development. The exogenous recombinant protein of HcTPS14, after catalyzing the substrate, can generate α-caryophyllene, a medicinal ingredient of sesquiterpene, which can be used to prepare α-caryophyllene and further prepare essential oils, essences and drugs; connect HcTPS14 to the plant transformation vector, and then introduce In Ginger Flower or other plant cells, transgenic plants expressing the gene can be obtained; specific molecular markers can also be produced according to the gene sequence information of the present invention, for identifying the sesquiterpene α-Caryophyllum of Ginger Flower or other plants The alkene synthase gene is used for molecular marker-assisted selection breeding, thereby improving the selection efficiency of breeding, and has great application prospects.