116results about How to "Reduce nitrite" patented technology

The invention relates to protein feed produced by multi-fungus fermentation with steam explosion rapeseed meal and cotton meal and a method. Rapeseed meal and cotton meal are used as main materials, meat and bone meal, straw and pomace are used as auxiliary materials, and a group of oxic liquid strain and anaerobic liquid strain are inoculated, so the protein feed is obtained. The preparation method is as follows: raw materials are delivered to a steam explosion system to be exploded, and then are released to normal pressure quickly after steam explosion; the materials after steam explosion are delivered to chelation reaction granary and cooled to 30 DEG C, and then are sterilized by microwave. The sterilized materials are delivered to a fermentation granary and the group of the oxic liquid strain is inoculated at the same time, the inoculum concentration is 5 percent, the intermittent ventilation and oxygen supplying are performed, the oxic fermentation time is between 36 and 48 hours; after the oxic fermentation, an anaerobic strain is inoculated, the inoculum concentration is 5 percent, and the sealed anaerobic culture is performed; and when the peptides content reaches 3 percent, the fermentation ends. The protein feed produced by multi-fungus fermentation with steam explosion rapeseed meal and cotton meal and the method have the advantages that toxin of miscellaneous mealis degraded completely after the steam explosion and microorganism fermentation treatment; the protein feed has flavor taste, and contains active substances, such as large amount of B vitamins, a plurality of vezyme and peptides, and so on; in addition, the protein feed has the functions of promoting growth development, resisting diseases.

The invention relates to a method for fermentation production of a poultry bacterial agent by multi-bacteria miscible liquid, wherein aerobic bacteria, namely Bacillus subtilis, Bacillus licheniformis, bacillus natto, beer yeast, Aspergillus niger and Aspergillus oryzae are cultured in a shaking table according to different culture mediums, so as to culture a mother seed solution; simultaneously Lactobacillus acidophilus, bifidobacteria and enterococcus faecalis are subjected to anaerobic culture by utilization of Kille flasks, and a mother bacterial solution of photosynthetic bacteria is cultured by a Kille flask under the condition of illumination; the prior stock solution is inoculated into a seed tank for anaerobic culture and fermentation according to 4 percent of the inoculum concentration, and the fermentation time is between 48 and 60 hours; and the cultured seed liquid is inoculated into a productive tank for anaerobic culture and fermentation according to 10 percent of the inoculum concentration, the fermentation time is between 60 and 72 hours, and the fermentation end point is reached when the pH value is reduced to 4.0. The method has the advantages that the microscopic examination viable count of the bacterial agent is more than 5 billion per milliliter, so that the bacterial agent is safe and nontoxic, thereby not only improving the disease resistance of poultry but also promoting the quick growth of the poultry, reducing the feed-meat ratio and improving the quality of meat, eggs and milk.

The invention belongs to the field of agriculture, which relates to a slow control release biological inorganic and organic mixed fertilizer. The mixing fertilizer is prepared by fully mixing the following components in percentage by weight: 3%-10% of beneficial microbial agent, 0.4%-1% of fertilizer slow control release agent, 5%-12% of organic matter, 0.2%-10% of plant growth stimulin, 15%-25% of nitrogen, 5%-12% of phosphorus, 6%-15% of potassium, and 2%-10% of middle trace element. The invention not only has the advantages of high nutrient concentration and simple production process, but also can enhance the utilization rate of the fertilizer, save the fertilizer, increase the output, improve the soil, and promote the sustainable development of agriculture and environment protection.

The invention discloses a compound microecological preparation used for water body purification of aquaculture. The compound microecological preparation is prepared by taking photosynthetic bacteria, bacillus amyloliquefaciens and denitrifying bacteria thalli as active constituents with a volume proportioning of 1:2:1 of fermentation liquors of the photosynthetic bacteria, the bacillus amyloliquefaciens and the denitrifying bacteria thalli. The invention is characterized in that the compound microecological preparation can obviously reduce the content of substances of ammonia nitrogen, nitrate nitrogen, nitrite nitrogen, COD and the like, in aquaculture water body. In the invention, strains have many types, rich quantity and strong environmental adaptivity, the three contained bacteria are all live bacteria; and the strains contain rich protein and vitamin and can also secrete multiple types of enzymes and anti-bacteria substances to promote cultivated living beings to grow and improve immunity of the cultivated living beings. Therefore, the invention can not only effectively purify the aquaculture water body, promote beneficial algae to reproduce and improve water body ecological environment, but also improve yield and quality of fish and shrimp; moreover, the invention is harmless to people and livestock, and can be produced in scale to be used for improving aquaculture environment.

The invention relates to a water purification microbial inoculum which is produced by blending and fermenting a plurality of bacteria. Straws are taken as raw materials and then seed solutions of a plurality of bacteria, a straw fermention medium and a concentrated nitrifying bacteria solution are added, thus obtaining the water purification microbial inoculum. A preparation method of the water purification microbial inoculum comprises the steps as follows: the straws are sent to a material steam explosion system and then a steam explosion is conducted to the straws; after the steam explosion, the straws are sent to a chelated reaction storage for reaction; after reacting in the chelated reaction storage, the straws are sent to a fermention storage for fermentation; 5 percent of the seed solution of a plurality of sporos and 30 percent to 40 percent of the straw fermention medium are added to the fermention storage; and before the straws are taken out of the storage, 2 percent to 3 percent of the concentrated nitrifying bacteria solution is added and stirred evenly, thereby obtaining the water purification microbial inoculum. The water purification microbial inoculum has the advantages that the valid viable bacteria number of the produced water purification microbial inoculum reaches 2 billion/g (slab) and nitration and subnitration can be realized; a purifier has comprehensive water purification functions which can effectively restrain the harmful bacteria of a cultured water body, thereby realizing the disease prevention and survival rate improvement; the degradation of the organic pollutant in the water body can be accelerated; and ammonia nitrogen can be decreased and dissolved, and oxygen can be increased, thereby achieving the aims of increasing production and income.

The invention provides a production method of a microbial fish guano. The production method comprises the following steps: fermenting materials, drying, crushing, and adding inorganic fertilizer and solid photosynthetic bacteria. Because all the organic fertilizers are fermented, the invention has a good water solubility and can fast culture pelagic organisms in the water substance, thereby providing adequate feedstuff to fishes and shrimps; the invention improves the culture water environment, and reduces the harmful substances in water, such as ammonia, nitrogen, nitrite, hydrogen sulfide and the like; the invention can realize the intensive alternate-breeding culture and greatly increase the yield per unit culture area, and the yield can be increased by more than 40% based on the current service condition; and the invention has the advantages of low cost, safety, high efficiency, environmental friendliness and no pollution, thereby ensuring the production of pollution-free green food.

The invention relates to a method for preparing a water quality modifying microecological preparation for reducing ammonia nitrogen and nitrite nitrogen, and belongs to the technical field of aquiculture technology and ecological environmental protection. The microecological is prepared by the following steps: mixing Bacillus foecalis alkaligenes CGMCC NO.1.1786 and arthrobacterium CGMCC NO.1282 into mixed bacteria; performing fermentation on the mixed bacteria for 24 to 36 hours in a culture medium in a fermenter at the fermentation temperature of between 28 and 32 DEG C; processing the obtained product into a liquid preparation through filling or continuous dilution or concentration after fermentation culture; and preparing the water quality modifying microecological preparation with the functions of reducing the ammonia nitrogen and the nitrite nitrogen in a water body. The preparation is convenient to use, is slightly influenced by environmental conditions, has small dosage, can obviously reduce the content of the ammonia nitrogen and the nitrite nitrogen in a pool water body after use, maintains the effect for 10 to 15 days, can effectively prevent diseases and paroxysmal death of aquaculture organisms and improve the yield and the quality of the aquaculture organisms when simultaneously improving the environmental quality of the pool water quality and the survival conditions of the aquaculture organisms.

The invention relates to a superoxide dismutase radish and the preparing method, seed dressing with SOD special-purpose agent for radish before sowing, managing according to normal planting management condition, spraying with 800-1200 times solution of the SOD special-purpose agent 15 days after the emergence of seedlings, one time for per 15-20 days, and for 4-6 times, then getting the fruit. Employing Yiwei SOD special-purpose agent on radish, and increasing the SOD activity and content in radish through the breeding and shifting of the good endogenetic bacillus in radish. The SOD radish maintains the good property of the regional radish, but the SOD content is obviously increased, which can improve the human immunity and delaying aging, for which the market prospect of the SOD radish is bright , and the radish fits the developing direction of green food and functional food. The radish maintains high SOD activity by employing the special-purpose SOD agent, and also can maintain high SOD activity during additional processing.

The invention provides a microecological preparation for purifying water quality in litopenaeus vannamei culture and preparation and application thereof, and relates to aquaculture. The microecological preparation for purifying water quality in litopenaeus vannamei culture contains at least one kind of materials from bacillus subtilis, bacillus pumilus and at least one kind of materials from lactobacillus plantarum and lactobacillus acidophilus; the microecological preparation for purifying water quality in litopenaeus vannamei culture comprises veterinary medicine, feed additives or water quality purification agents and the like. The preparation applied to the litopenaeus vannamei culture is concretely a preparation prepared from bacillus subtilis, bacillus pumilus, lactobacillus plantarum and lactobacillus acidophilus, and is applied in aspects of promoting litopenaeus vannamei growth, improving the immunity and the disease resistant capability, improving the litopenaeus vannamei culture survival rate, simultaneously reducing the ammonia nitrogen in the culture water body and purifying the culture water quality.

The invention provides a formula of floc nutrients for cultivating bio-flocs and beneficial bacteria as well as a preparation and an application. The formula is characterized in that the formula comprises the following components in percentage by weight: 20-40% of puffed corn flour, 5-10% of rice husk powder, 5-10% of humic acid, 40-60% of molasses powder and 10-20% of beneficial bacteria powder; wherein the beneficial bacteria is 1x10<8>-1x10<10> cell/g, and comprises Bacillus cereus, Bacillus licheniformis, Bacillus subtilis, Lactobacillus, and Lactobacillus acidophilus. The floc nutrients are widely applied to water bodies for cultivating prawns, sea cucumbers and fishes, so that formation of bio-flocs in the water bodies is accelerated, ammonia nitrogen and nitrous nitrogen and other harmful substances are rapidly reduced, and directed breeding of beneficial bacteria in the water bodies are improved. The floc nutrients are green and environmentally friendly, and have the advantages of substantially improved production benefits and reduced production cost, wide market prospects, usage convenience; only water is needed for dilution of the floc nutrients, and the diluted floc nutrients are sprayed into cultivation water once a day.

The invention discloses a biological water purification agent powder and a preparation thereof, belonging to the technical field of microorganism environment protection. The biological water purification agent powder comprises 35-55 wt% of composite bacillus, 10-20 wt% of nitrobacteria and 25-55 wt% of auxiliary material. In the preparation of the biological water purification agent powder, a primary solid content separator is used for removing 60-70% of water, a secondary solid content separator is used for obtaining a formed solid content with the solid content being 30-45%, and finally mixing preparation is carried out to obtain the finished product. The biological water purification agent powder can be used in purification treatment of contaminated water to improve the water quality, and the substrate sludge is decomposed to provide an excellent water quality environment for aquaculture animals.

The invention discloses flavored tea-pork sausages and processing methods thereof. The flavored tea-pork sausages are processed and made by using pork as a main raw material and using tea liquid, fruit, fermented vegetables, a spice extracting solution and the like as main auxiliary materials. According to different used materials, the invention provides formulas of three different flavors of flavored tea-pork sausages. According to different product storage methods, the invention provides methods for processing three types of products, namely a dried flavored tea-pork sausage, a refrigerated type ripened flavored tea-pork sausage and a normal-temperature stored type ripened flavored tea-pork sausage. Through the adding of the tea liquid, the fruit and the fermented vegetables in the processing of the sausages, the growth of beneficial microorganisms, such as lactobacilli, can be promoted in the ripening process of the sausages, and the growth and the reproduction of putrefying bacteria and harmful microorganisms and the production and the accumulation of nitrite can be inhibited in the processing process of the sausages. Meanwhile, the sausages can be specially flavored, the greasy feeling of the sausages is reduced, and the nutrients of the sausages are more balanced.

The invention provides a black odor water treatment method based on biochar immobilization composite microorganism treatment and an aeration reoxygenation system of the black odor water treatment method. The treatment method comprises the following steps: (1) surveying the river pollution profile, (2) performing source control and sewage interception, (3) performing aeration and reoxygenation treatment, (4) performing degradation and purification treatment by using a biochar immobilization composite microorganism system, and (5) performing comprehensive monitor and long-term dynamic assessment on the health of an urban river ecosystem. Input of energy materials is small, man-made management control is less, economical efficiency is achieved, and the cost is only 30-50% of the cost of a traditional environmental engineering technology. According to the immobilization composite microorganism ecological restoration technology, natural organisms or slightly modified organisms rather than artificial substances are utilized to purify sewage in rivers and lakes, the environmental compatibility is good, and no secondary pollution to the environment is caused; and the method can be combined with hydrophilic landscape construction, and the external forms are natural, and is more humanized.

A sono-molecular-conversion device and method for effecting nitrification of ammonia in water, the method including feeding the water containing ammonia through a sono-molecular conversion device including a plurality of ultrasound transducers; and applying ultrasonic energy to the water containing ammonia by the sono-molecular conversion device to ultrasonically collapse microsized bubbles with transient cavitation in the water containing ammonia to effect nitrification of ammonia therein by sono-molecular-conversion.

The invention discloses fermented-flavor tea fish sausage and a processing method thereof. The fermented-flavor tea fish sausage is prepared by taking minced fillet and pork as main raw materials and the tea liquid, fruit, fermentation vegetable, spice extracting solutions and the like as main accessories, and inoculating the pure-culture mixed strain of lactic acid bacteria, saccharomycetes and micrococcus for fermentation. According to different materials, the invention provides three formulas of the fermented-flavor tea sausage with different flavors. According to different product preservation methods, the invention provides the processing method of two products of cold-storage cooking-flavor tea sausage and normal temperature-storage cooking-flavor tea sausage. By adding the tea liquid, fruit, fermentation vegetable and fermentation strains in sausage processing, the growth of beneficial microorganisms such as lactic acid bacteria and the like can be promoted in the sausage maturation process, and the growth and reproduction of harmful microorganisms are inhibited while inhibiting the generation and accumulation of nitrite, thereby improving the edible safety; meanwhile, the sausage has a specific flavor, the nutrition of the sausage tends to balance, and the oiliness of the sausage is reduced.

The invention discloses a flavor tea fish sausage and a processing method thereof. The flavor tea fish sausage is prepared from minced fish and pork as main raw materials and tea liquid, fruits, fermented vegetables, spice extracting liquid and the like as main auxiliary materials. According to different materials, formulae of three flavor tea fish sausages with different flavors are provided. According to different product storage methods, the invention provides processing methods of a drying type flavor tea fish sausage, a refrigeration type cooked flavor tea fish sausage and a normal-temperature storage type cooked flavor tea fish sausage. The tea liquid, the fruits and the fermented vegetables are added in the sausage processing process, so that the growth of beneficial microorganisms such as lactic acid bacteria in the sausage cooking process can be promoted, the growth and the propagation of putrefying bacteria and harmful microorganisms in the sausage processing process and the generation and the accumulation of nitrite can be inhibited, the eating safety is high, the sausage can be endowed with a unique flavor, the greasiness of the sausage is reduced, and the nutrition of the sausage can tend to be balanced.

The invention discloses a complex microbial inoculant for improving the aquaculture pond bottom mud environment and a preparation method and application thereof. The complex microbial inoculant is composed of bacterial powder 1, bacterial powder 2 and bacterial powder 3. The bacterial powder 1 is Thermochromatium tepidum ATCC 43061. The bacterial powder 2 is Thioalkalivibrio denitrificans DSM 13742. The bacterial powder 3 is Achromobacter denitrificans DSM 30026 and Achromobacter xylosoxidans subsp.denitrificans ATCC15173. The weight ratio of the bacterial powder 1 to the bacterial powder 2 to the bacterial powder 3 is 1-2 to 1-2 to 1-2. The complex microbial inoculant can deeply permeate into the bottom of a pond, quickly and efficiently degrade residual feeds, excrement, flora and fauna dead bodies and organic detritus depositing to the bottom of the pond, remarkably reduce ammonia nitrogen, nitrite, sulfide and other hazardous substances at the bottom of the pond, and then achieves the purposes of improving the pond bottom mud environment and purifying the water.

The present invention relates in one aspect to a method for producing a transgenic plant, comprising introducing into an unmodified plant an exogenous gene encoding a nitrite reductase, wherein expression of the nitrite reductase encoded by the exogenous gene reduces nitrite content in the transgenic plant relative to the unmodified plant. Also provided are transgenic plants and plant cells comprising an exogenous gene encoding a nitrite reductase, as well as associated uses, chimaeric genes and plant transformation vectors.

The present invention discloses a preparation method of sea cucumber biologically fermented baits. Marina pseudoalteromonas and mixed effective microorganisms (EM) are inoculated into the baits to be subjected to anaerobic fermentation at 25-35 DEG C for 3-7 days. The mixed EM consists of bacillus, photosynthetic bacteria, yeasts and lactobacillus. The method comprises the step of conducting solid state fermentation on the stichopus japonicus baits via the microorganisms to prepare the fermented baits. Compared with the unfermented baits, the prepared baits are significantly increased in the attractiveness to the stichopus japonicus. Besides, the fermented baits are more comprehensive in nutrition and more conductive to the growth of the stichopus japonicus, improve the survival rates of the stichopus japonicus, improve the intestinal flora of the stichopus japonicus, and improve the digestion and absorption rates of the baits by the stichopus japonicus. At the same time, due to the addition of seaweed gel degrading bacteria, the fermented baits can effectively degrade kelp powder rich in the seaweed gel and difficult to digest by the stichopus japonicus in the baits, so that the proportion of the kelp powder in the baits can be increased to replace parts of expensive sargassum and the purposes of lowering enterprise costs is acheived.

It has been found that the urine from an IC patient shows a high value in amount and the existence of activity of an azurophilic granular substance, thereby to establish a method for examining IC. The present invention relates to a method for examining interstitial cystitis using the kinetics of an azurophilic granular substance in urine as a marker. Also, the present invention relates to a kit for examining interstitial cystitis for use in the examination method, a use of an azurophilic granular substance as a test marker for examining interstitial cystitis or evaluating pharmacological effects of a drug, and a method for examining therapeutic effects on a patient with interstitial cystitis using an azurophilic granular substance as a marker.

The invention discloses a method for producing a biological water-purifying agent through microbe mutual fermentation of chicken manure. The method comprises: mixing 50-65% of fresh chicken manure, 20-30% of pleurotus eryngii residues, and 15-20% of an inorganic compound fertilizer, and inoculating Aspergillus oryzae, a yeast, a photosynthetic bacterium and bacillus subtilis into the mixture for fermentation, which can generate an effective microbe number of 3-4*10<8>CFU/g. Specifically, the inoculated strains are: 2-6 wt% of a solid strain of Aspergillus oryzae ACCC30155, 1-2 wt% of a liquidstrain of saccharomyces cerevisiae ACC20065, 1-2 wt% of a liquid strain of bacillus subtilis ACCC10148, and 1-2 wt% of a liquid strain of photosynthetic bacterium ACCC10650. After 15-20d of stacking fermentation, the biological water-purifying agent can be obtained. And the produced biological water-purifying agent of the invention can be prepared into different water purifying agents and aquaculture products according to needs, thus boasting wide application prospects in agriculture, aquaculture industry and environmental protection industry.