Method for producing biological water-purifying agent through microbe mutual fermentation of chicken manure

A technology for fermenting chicken manure and water purifying agent is applied in the field of agricultural waste utilization, which can solve the problems of livestock and poultry manure pollution and increase urban pressure, and achieve the effects of improving water quality, high efficiency and high conversion rate.

Active Publication Date: 2012-04-04
长沙浩博生物技术有限公司
View PDF3 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most livestock and poultry farms do not treat manure, so the pollution of livestock and poultry manure is increasing the pressure on cities and towns

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for producing biological water-purifying agent through microbe mutual fermentation of chicken manure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The effective microbial strains in the fermentation process of chicken manure were Aspergillus oryzae [ACCC30155], Saccharomyces cerevisiae [ACC20065], Bacillus subtilis [ACCC10148] and photosynthetic bacteria [ACCC10650], all purchased from China Agricultural Microbiology Center.

[0015] Aspergillus oryzae and yeast medium: 10% wort medium, no agar is added as a liquid medium, and 2% agar is added as a solid medium.

[0016] Bacillus subtilis culture medium: nutrient broth, liquid medium without agar, solid medium with 2% agar.

[0017] Photosynthetic bacteria medium: PSB medium, CH 3 COONa 3.0g·L -1 , (NH 4 ) 2 SO 4 , 1.5g·L -1 , MgSO 4 ·7H 2 O0.8g L -1 , KH 2 PO 4 0.3g·L -1 , K 2 HPO 4 0.5g·L -1 , yeast extract powder 1.2g·L -1 , pH7.5, no agar is used as a liquid medium, and 2% agar is added as a solid medium.

[0018] Inoculate the above-mentioned strains on the corresponding slopes for activation, and then inoculate them into the corresponding Erl...

Embodiment 2

[0026] The activation culture of the strain is the same as in Example 1.

[0027] 55wt% fresh chicken manure, 27% Pleurotus eryngii fungus chaff, 18% inorganic compound fertilizer solid raw material are mixed uniformly, then inoculated with Aspergillus oryzae 6wt% accounting for solid raw material gross weight, saccharomyces cerevisiae 2wt%, Bacillus subtilis 2wt% and 2 wt% of photosynthetic bacteria, then the thickness of the material is kept at 50-60 cm, stacked and fermented at normal temperature for 15 days, and stirred twice during the fermentation process to obtain the product of the present invention. The number of effective microorganisms after fermentation is about 4×10 8 CFU / g, according to different users, inorganic fertilizers can be added to make products with different nutrients.

[0028] Get 100 kilograms of above-mentioned products, add inorganic compound fertilizer (nutrient is 17%N on the market: the product of 5%P: 8%K all can) 20 kilograms are mixed, both ...

Embodiment 3

[0031] The activation culture of the strain is the same as in Example 1.

[0032] 55wt% fresh chicken manure, 27% Pleurotus eryngii fungus chaff, 18% inorganic compound fertilizer solid material are mixed uniformly, then inoculate 2wt% of Aspergillus oryzae accounting for the total weight of solid material, 1wt% of Saccharomyces cerevisiae, 1wt% of Bacillus subtilis and 1 wt% of photosynthetic bacteria, then the thickness of the material is kept at 50-60 cm, stacked and fermented at normal temperature for 15 days, and stirred twice during the fermentation process to obtain the product of the present invention. The effective number of microorganisms after fermentation is 3×10 8 CFU / g, according to different users, inorganic fertilizers can be added to make products with different nutrients.

[0033] Get 100 kilograms of above-mentioned products, add inorganic compound fertilizer (nutrient is 17%N on the market: the product of 5%P: 8%K all can) 15 kilograms mix, can obtain the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for producing a biological water-purifying agent through microbe mutual fermentation of chicken manure. The method comprises: mixing 50-65% of fresh chicken manure, 20-30% of pleurotus eryngii residues, and 15-20% of an inorganic compound fertilizer, and inoculating Aspergillus oryzae, a yeast, a photosynthetic bacterium and bacillus subtilis into the mixture for fermentation, which can generate an effective microbe number of 3-4*10<8>CFU/g. Specifically, the inoculated strains are: 2-6 wt% of a solid strain of Aspergillus oryzae ACCC30155, 1-2 wt% of a liquidstrain of saccharomyces cerevisiae ACC20065, 1-2 wt% of a liquid strain of bacillus subtilis ACCC10148, and 1-2 wt% of a liquid strain of photosynthetic bacterium ACCC10650. After 15-20d of stacking fermentation, the biological water-purifying agent can be obtained. And the produced biological water-purifying agent of the invention can be prepared into different water purifying agents and aquaculture products according to needs, thus boasting wide application prospects in agriculture, aquaculture industry and environmental protection industry.

Description

technical field [0001] The invention belongs to the technical field of agricultural waste utilization, in particular to producing a biological water purifying agent by transforming livestock and poultry manure through microbial fermentation, and then preparing a water body purifying agent or aquaculture product for water cultivation and fertilization. Background technique [0002] In recent years, large-scale, intensive, and modern farms have shown a sharp upward trend. Due to unsound environmental regulations and shortage of funds, the issue of manure disposal is often not considered at the beginning of farm construction. According to measurements, a factory chicken farm that raises 100,000 chickens can produce up to 10 tons of chicken manure per day, and an annual output of more than 3,600 tons of chicken manure. Most livestock and poultry farms do not treat the manure, so the pollution of livestock and poultry manure is increasing the pressure on cities and towns. The ha...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/18C12N1/20C02F3/34C05F17/00C12R1/01C12R1/125C12R1/69C12R1/865
CPCY02W30/40
Inventor 谭周进邓悟森丁祥力伍兵
Owner 长沙浩博生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap