The invention discloses a flour with multiple microelement supplements and a preparation method of the flour. The flour with multiple microelement supplements comprises the following raw materials inparts by mass: wheat flour, sorghum flour, corn flour, soybean flour, sweet potato powder, egg yolk powder, organic spinach powder, chestnut powder, walnut powder, cabbage powder, grape powder, peanutpowder, potato peel powder, apple powder, banana powder, oyster powder, degummed kelp powder, laver powder, wheat germ powder, indica rice flour, asparagus, sesame powder, garlic powder, vitamin B1,vitamin B2, vitamin B6, vitamin B12, health-care additives, color mixing powder, fungus additives. According to the present invention, the flour with multiple microelement supplements has the following beneficial effects that the flour with multiple microelement supplements is more conducive to human absorption by entering the gastrointestinal tract with digestive protease, has no damage to nutrient carriers when used, transports organic substances to required organs after entering the gastrointestinal tract, and effectively supplement calcium, iron, zinc, selenium, vitamins and other nutrients that play an important role in the human body.