The invention belongs to the technical field of biological control, and relates to application of 
Wickerhamomyces anomalus in 
postharvest disease control, storage and fresh-keeping of tomatoes. A method comprises the following steps of: activating and culturing 
Wickerhamomyces anomalus, performing centrifuging to obtain thalli, diluting the thalli with 
sterile water to obtain a 
yeast suspension with the concentration of 1*10<8> cells / mL, 
punching the surfaces of the tomatoes, injecting the 
yeast suspension, and injecting an isopyknic 
botrytis cinerea 
spore suspension with the concentration of 1*10<5> spores / mL after 2 hours, so as to effectively control the gray mold, or directly and uniformly spraying the 
yeast suspension on the surfaces of the tomatoes, and performing natural air-
drying. The purposes of preventing and treating the 
postharvest gray mold of the tomatoes, storing and fresh-keeping can be realized; according to the invention, 
postharvest gray mold and natural rot of the tomatoes can be effectively controlled, so that the loss caused by postharvest diseases of the tomatoes is reduced; meanwhile, operation is convenient, safe and environment-friendly; and remarkable 
economic benefits and 
social benefits are achieved.