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Compound microecological preparation used for water body purification of aquaculture

A composite micro-ecology and water purification technology, applied in the direction of microorganism-based methods, microorganisms, microorganisms, etc., to achieve the effects of strong environmental adaptability, abundant quantity, and many types of bacteria

Inactive Publication Date: 2010-06-02
TIANJIN CITY AGRI BIO TECH RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Photosynthetic bacteria and denitrifying bacteria have been widely used in aquaculture as single and compound bacterial agents, but from the analysis of existing literature and patents, there is no use of Bacillus amyloliquefaciens, photosynthetic bacteria and denitrifying bacteria in combination for aquaculture. Research Report on Purification of Breeding Water

Method used

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  • Compound microecological preparation used for water body purification of aquaculture
  • Compound microecological preparation used for water body purification of aquaculture
  • Compound microecological preparation used for water body purification of aquaculture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1: Research on the Compounding Ratio of Compound Probiotics

[0014] Three dosages of photosynthetic bacteria, Bacillus amyloliquefaciens and denitrifying bacteria were set up for orthogonal combination, and the three levels of photosynthetic bacteria were A1 (10mL), A2 (20mL) and A3 (30mL), respectively. Bacillus 3 levels are respectively B1 (20mL), B2 (40mL), B3 (60mL), denitrifying bacteria 3 levels are respectively C1 (10mL), C2 (30mL), C3 (50mL), carry out orthogonal combination, get Nine combinations with different compound ratios were used for water purification test. At the end of the test, an orthogonal analysis was carried out on each index, and the results are shown in Table 1.

[0015] Each water quality index at the end of the test in Table 1

[0016]

[0017] Note: Taking the degradation rate of CK as a reference, the degradation rate (increase rate) higher than the control is +, and the lower is -

[0018] The results of orthogonal analysis ...

Embodiment 2

[0019] Example 2: Verification test of water purification effect of compound probiotics

[0020] The screened superior combinations A1B1C1, A3B3C3, A1B1C2 and purchased commercial bacteria EM were tested for water purification verification. The water quality indicators measured at the end of the test are shown in Table 2.

[0021] Each water quality index at the end of the test in Table 2

[0022]

[0023] Note: Different letters in the same column indicate significant difference at P<0.05.

[0024] It can be seen from Table 2 that the combination of A1B1C1 can increase the dissolved oxygen and degrade COD and nitrite better than the combination of A1B1C2 and A3B3C3. The degradation rate of A1B1C1 combination on COD in simulated aquaculture water was 100% significantly better than 34.1% of EM treatment, and the degradation rates of ammonia nitrogen, nitrite and nitrate were 70%, 89% and 56% better than EM bacteria.

Embodiment 3

[0025] Example 3: Safety Evaluation of Compound Probiotics

[0026] A feeding test was carried out on the compound microecological preparation A1B1C1. Each tank was filled with 40 L of water, and 10 crucian carp (about 6-8 cm in length) of basically the same size were stocked. Each tank was inoculated with 1 L of the compound probiotic preparation, and the bacteria were injected once every 7 days. The period is 30d.

[0027] Table 3 Fry survival and weight gain

[0028]

[0029] During the test period, the fish fry were eating normally, very active, without floating head phenomenon, and no death phenomenon occurred, indicating that the compound microecological preparation was safe for fish, and the treatment of adding bacterial agents was beneficial to the growth of fry, and the weight of the fish treated with the compound probiotic preparation was relatively low. The control was 117g higher, an increase of 30.5%.

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Abstract

The invention discloses a compound microecological preparation used for water body purification of aquaculture. The compound microecological preparation is prepared by taking photosynthetic bacteria, bacillus amyloliquefaciens and denitrifying bacteria thalli as active constituents with a volume proportioning of 1:2:1 of fermentation liquors of the photosynthetic bacteria, the bacillus amyloliquefaciens and the denitrifying bacteria thalli. The invention is characterized in that the compound microecological preparation can obviously reduce the content of substances of ammonia nitrogen, nitrate nitrogen, nitrite nitrogen, COD and the like, in aquaculture water body. In the invention, strains have many types, rich quantity and strong environmental adaptivity, the three contained bacteria are all live bacteria; and the strains contain rich protein and vitamin and can also secrete multiple types of enzymes and anti-bacteria substances to promote cultivated living beings to grow and improve immunity of the cultivated living beings. Therefore, the invention can not only effectively purify the aquaculture water body, promote beneficial algae to reproduce and improve water body ecological environment, but also improve yield and quality of fish and shrimp; moreover, the invention is harmless to people and livestock, and can be produced in scale to be used for improving aquaculture environment.

Description

technical field [0001] The invention belongs to the technical field of biological bacteria preparations and the application field of agricultural microorganisms. In particular, it relates to a compound microecological preparation for water purification in aquaculture. Background technique [0002] With the continuous improvement of aquaculture intensification, the physical and chemical environment and ecological environment of aquaculture water are deteriorating day by day; at the same time, the excessive discharge of human sewage, various industrial wastewater, and agricultural dewatering has also exacerbated the decline in the quality of aquaculture water; These problems cause breeding diseases to become more and more serious. The abuse of antibiotics and chemicals not only enhances the drug resistance of pathogenic microorganisms, but also leads to excessive harmful residues in aquatic products, directly threatening human health. Countries and regions such as the United...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/20C02F3/34C12R1/01C12R1/07C12R1/38C02F101/16
Inventor 谢凤行赵玉洁周可张峰峰李亚玲
Owner TIANJIN CITY AGRI BIO TECH RES CENT
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