Microecological preparation for purifying water quality in litopenaeus vannamei culture and preparation and application thereof

A micro-ecological preparation, water purification technology, applied in the application, biocide, microorganism and other directions, can solve the problems of drug residues, economic losses of farmers, restricting the development of shrimp farming

Inactive Publication Date: 2017-06-20
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the high temperature season of late spring and summer every year, various acute infectious diseases often break out, such as nerve necrosis virus, iridescent virus, vibrio and other pathogenic bacteria, which cause a large number of deaths of shrimp seedlings and juvenile shrimp, bringing huge economic losses to farmers , seriously restricting the development of shrimp farming
According to statistics, the annual morta...

Method used

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  • Microecological preparation for purifying water quality in litopenaeus vannamei culture and preparation and application thereof
  • Microecological preparation for purifying water quality in litopenaeus vannamei culture and preparation and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 A kind of preparation of probiotics

[0043] Cultivation of bacteria of each component in probiotics

[0044] Bacillus subtilis (Bacillus subtilis 1A05680), preservation number CCTCC NO: M2013289 and Bacillus pumilus include the cultivation of Bacillus pumilus (Bacillus pumilus) 1A08151 preservation number CCTCC NO: M2013291: the cultivation of the starting bacterial strain Bacillus subtilis and Bacillus pumilus according to Sequential culture of slant, liquid seed, and liquid fermentation, slant and seed medium are conventional nutrient agar medium or broth medium; fermentation medium formula (W / V): 3% bean cake powder, 1.5% starch, corn steep liquor 1%, glucose 0.5%, (NH 4 ) SO 4 0.1%, KH 2 PO 4 0.5%, NaH 2 PO 4 0.5%, MgSO 4 0.05%, MnSO 4 0.05%, the fermentation condition is 28~37℃, the ventilation culture is 36~48h, the number of viable bacteria is ≥10 9 CFU / mL;

[0045] Lactobacillus plantarum includes Lactobacillus plantarum (Lactobacill...

Embodiment 2

[0051] Application test 1 of embodiment 2 probiotics of the present invention in prawn culture

[0052] 1. Materials and methods

[0053] 1. Test product: the compound probiotic preparation bacterium liquid of embodiment 1, the total number of viable bacteria is more than 1 billion / mL.

[0054] 2. Test prawns: divide the prawns into 2 groups, 250 tails in each group, and set 3 replicates in each group.

[0055] 3. Test water body: The test water is taken from sand-filtered seawater in Baicheng, Xiamen.

[0056] 4. Test site: The test is carried out in a half-ton glass fiber tank. The test cylinders were placed in an open place outdoors, randomly placed under natural conditions.

[0057] 5. Test method: The test period is 45 days. The test adopts conventional prawn feed feeding, and the test group adds 1 kg of test product per ton of feed to ensure that the number of viable bacteria in each gram of feed is ≥ 10 6 CFU / mL, no test product was added to the control group. On t...

Embodiment 3

[0069] The application test of the microecological preparation of the present invention in the culture of prawn shrimp fry of embodiment 3

[0070] 1. Test product: the compound probiotic preparation bacterium liquid of embodiment 1, the total number of viable bacteria is more than 1 billion / mL.

[0071] 2. Experimental prawns: The prawns from seedlings to 45 days old are used throughout. Set up 5 experimental pools and 5 control pools.

[0072] 3. Test site: a prawn farm in Zhanggang Town, Changle City.

[0073] 4, test method: when raising daily, get embodiment 1 compound bacterial agent pool and evenly splash. It is used once every three days, and the dosage of each mu of aquaculture water body is 0.5-1L, and it is doubled when the water quality deteriorates. The control group did not use the bacteria agent. Get the composite bacterial agent of embodiment 1 at the same time and evenly spray on the feed surface after diluting with appropriate amount of water, mix and fee...

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Abstract

The invention provides a microecological preparation for purifying water quality in litopenaeus vannamei culture and preparation and application thereof, and relates to aquaculture. The microecological preparation for purifying water quality in litopenaeus vannamei culture contains at least one kind of materials from bacillus subtilis, bacillus pumilus and at least one kind of materials from lactobacillus plantarum and lactobacillus acidophilus; the microecological preparation for purifying water quality in litopenaeus vannamei culture comprises veterinary medicine, feed additives or water quality purification agents and the like. The preparation applied to the litopenaeus vannamei culture is concretely a preparation prepared from bacillus subtilis, bacillus pumilus, lactobacillus plantarum and lactobacillus acidophilus, and is applied in aspects of promoting litopenaeus vannamei growth, improving the immunity and the disease resistant capability, improving the litopenaeus vannamei culture survival rate, simultaneously reducing the ammonia nitrogen in the culture water body and purifying the culture water quality.

Description

technical field [0001] The invention relates to aquaculture, in particular to a microecological preparation for purifying water quality in Litopenaeus vannamei culture, a preparation method and application thereof. Background technique [0002] Since 2003, with the promotion of high-density farming mode, my country's shrimp farming industry has entered a period of rapid development. In 2006, the total amount of shrimp farming in my country was 1.25 million tons, accounting for 39.2% of the global farmed shrimp production, and it has remained the world's first for many years . However, the rapid development of intensive and high-density aquaculture has seriously damaged the ecological environment of aquaculture, and also produced a series of negative effects, including waste of feed resources, environmental deterioration, serious diseases, germplasm degradation, drug residues, etc. , especially disease problems are becoming more and more serious. Shrimp white spot virus, tau...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34A61K35/742A61K35/747A61P3/00A61P37/04A61P31/14A61P31/20A23K50/80A23K10/18A01N63/02A01P1/00C12R1/125C12R1/07C12R1/25C12R1/23C02F101/16C02F103/20
CPCA01N63/10A61K35/742A61K35/747C02F3/348C02F2101/16C02F2103/20C02F2303/04C12N1/20A61K2300/00Y02A40/818
Inventor 邵宗泽单大鹏李旭林剑辉王丽萍
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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