Microecological preparation for purifying water quality in litopenaeus vannamei culture and preparation and application thereof
A micro-ecological preparation, water purification technology, applied in the application, biocide, microorganism and other directions, can solve the problems of drug residues, economic losses of farmers, restricting the development of shrimp farming
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0042] Embodiment 1 A kind of preparation of probiotics
[0043] Cultivation of bacteria of each component in probiotics
[0044] Bacillus subtilis (Bacillus subtilis 1A05680), preservation number CCTCC NO: M2013289 and Bacillus pumilus include the cultivation of Bacillus pumilus (Bacillus pumilus) 1A08151 preservation number CCTCC NO: M2013291: the cultivation of the starting bacterial strain Bacillus subtilis and Bacillus pumilus according to Sequential culture of slant, liquid seed, and liquid fermentation, slant and seed medium are conventional nutrient agar medium or broth medium; fermentation medium formula (W / V): 3% bean cake powder, 1.5% starch, corn steep liquor 1%, glucose 0.5%, (NH 4 ) SO 4 0.1%, KH 2 PO 4 0.5%, NaH 2 PO 4 0.5%, MgSO 4 0.05%, MnSO 4 0.05%, the fermentation condition is 28~37℃, the ventilation culture is 36~48h, the number of viable bacteria is ≥10 9 CFU / mL;
[0045] Lactobacillus plantarum includes Lactobacillus plantarum (Lactobacill...
Embodiment 2
[0051] Application test 1 of embodiment 2 probiotics of the present invention in prawn culture
[0052] 1. Materials and methods
[0053] 1. Test product: the compound probiotic preparation bacterium liquid of embodiment 1, the total number of viable bacteria is more than 1 billion / mL.
[0054] 2. Test prawns: divide the prawns into 2 groups, 250 tails in each group, and set 3 replicates in each group.
[0055] 3. Test water body: The test water is taken from sand-filtered seawater in Baicheng, Xiamen.
[0056] 4. Test site: The test is carried out in a half-ton glass fiber tank. The test cylinders were placed in an open place outdoors, randomly placed under natural conditions.
[0057] 5. Test method: The test period is 45 days. The test adopts conventional prawn feed feeding, and the test group adds 1 kg of test product per ton of feed to ensure that the number of viable bacteria in each gram of feed is ≥ 10 6 CFU / mL, no test product was added to the control group. On t...
Embodiment 3
[0069] The application test of the microecological preparation of the present invention in the culture of prawn shrimp fry of embodiment 3
[0070] 1. Test product: the compound probiotic preparation bacterium liquid of embodiment 1, the total number of viable bacteria is more than 1 billion / mL.
[0071] 2. Experimental prawns: The prawns from seedlings to 45 days old are used throughout. Set up 5 experimental pools and 5 control pools.
[0072] 3. Test site: a prawn farm in Zhanggang Town, Changle City.
[0073] 4, test method: when raising daily, get embodiment 1 compound bacterial agent pool and evenly splash. It is used once every three days, and the dosage of each mu of aquaculture water body is 0.5-1L, and it is doubled when the water quality deteriorates. The control group did not use the bacteria agent. Get the composite bacterial agent of embodiment 1 at the same time and evenly spray on the feed surface after diluting with appropriate amount of water, mix and fee...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com