The invention relates to loach settling-type puffing mixed feed and a preparing method thereof, and belongs to the technical field of puffing feed production used for aquatic products. The feed comprises the following raw materials, that is, fish meal, meat meal, corn gluten meal, spray-dried animal blood cells, fermented soybean meal, soybeans, bean pulp, rapeseed dregs, DDGS, flour, soybean oil, monocalcium phosphate, bentonite, 98.5% lysine hydrochloride, 99% DL methionine, choline chloride, decavitamin premix compound, 35% vitamin C phosphate, composite mineral substance premix compound and sodium butyrate. The loach settling-type puffing mixed feed can well maintain intestinal health of loaches, further improves the nutrition utilization rate, invigorates health and improves anti-stress capacity of the loaches. The prepared loach settling-type puffing mixed feed is used in breeding stage of the loaches, the ingestion characteristics of the loaches are combined, the ingestion and growth states of the loaches are good, the physique of the loaches is strong, the feed coefficient is obviously reduced than that of an existing popular hard particle feed product, the survival rate is obviously improved and culture benefits are substantially improved.