Nutrient-degrading enzyme, engineering bacterial agent and application thereof in nutrient regulation of polymeric slow-release fertilizer

By using artificial intelligence-modified specific nutrient-degrading enzymes and composite engineered strains, the problem of low degradation efficiency of polymeric slow-release fertilizers has been solved, achieving efficient and stable nutrient supply and increased crop yield, making it suitable for precision agriculture.

CN121592626BActive Publication Date: 2026-06-09SHANDONG AGRICULTURAL UNIVERSITY
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Patent Information

Application Number
CN202610120933.7
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2026-01-29
Publication Date
2026-06-09
Estimated Expiration
2046-01-29

AI Technical Summary

Technical Problem

Existing polymeric slow-release fertilizers have low degrading enzyme activity, poor thermal stability, and insufficient substrate binding efficiency, making it difficult to meet the needs of large-scale agricultural production. Furthermore, the engineered strains have poor stability, making it difficult to achieve complete degradation of polymeric slow-release fertilizers.

Method used

A specific nutrient-degrading enzyme modified by artificial intelligence was developed. The enzyme molecule modification model was constructed through deep learning, the amino acid sequence of pepE and amiE proteins was optimized, and a composite engineered strain was constructed by combining it with engineered bacterial agents to achieve efficient heterologous expression and improved stability. Furthermore, precise nutrient supply was achieved by adjusting the ratio of peptidase to amidase.

Benefits of technology

It achieves efficient degradation of polymeric slow-release fertilizer, promotes precise supply of nutrients during crop growth, improves crop yield and nutrient utilization, reduces fermentation costs, and has the potential for large-scale application.

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Abstract

This invention discloses a nutrient-degrading enzyme, engineered microbial agents, and their application in the nutrient regulation of polymeric slow-release fertilizers, belonging to the field of enzyme engineering technology. This invention utilizes a specific polymeric slow-release fertilizer-degrading enzyme modified by artificial intelligence, which not only retains the degradation activity of the source strain enzyme but also achieves efficient heterologous expression while promoting a dual improvement in stability and catalytic efficiency. The constructed composite engineered strain exhibits no antagonistic effect, enabling low-cost, large-scale production of the degrading enzyme. The composite engineered strain can achieve precise regulation of nutrient release from polymeric slow-release fertilizers. By adjusting the ratio of engineered strains producing peptidases and amidases, precise supply of nutrients to different stages throughout the growth cycle of different crops can be achieved, resulting in complete degradation of polymeric slow-release fertilizers, significantly improving crop yield and nutrient utilization, and showing significant application prospects in the development of precision agriculture.
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