Preparation method of sea cucumber biologically fermented baits

A technology of biological fermentation and biological bait, applied in animal feed, animal feed, additional food elements, etc., can solve the problems of lack of sea cucumber nutrition and bait research, unsatisfactory natural bait, hinder the rapid growth of sea cucumber, etc., and achieve a large economy Significance and social value, effect of increasing utilization and reducing morbidity

Inactive Publication Date: 2016-10-26
DONGGUAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the rapid development of intensification, scale and automation of the sea cucumber farming industry, the limited natural bait can no longer meet the needs of the actual market
However, there is still a lack of systematic research on the

Method used

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  • Preparation method of sea cucumber biologically fermented baits
  • Preparation method of sea cucumber biologically fermented baits
  • Preparation method of sea cucumber biologically fermented baits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Step a, marine pseudoalteromonas culture:

[0062] The fermentation broth of Pseudomonas marine has been prepared above.

[0063] Step b, mixed EM bacterial culture:

[0064] Take the seed liquids of the four strains of Bacillus, photosynthetic bacteria, yeast and lactic acid bacteria prepared above, and inoculate them into the mixed EM bacteria fermentation medium with v / v inoculums of 0.4%, 0.6%, 0.4%, and 0.6% respectively , under the conditions of 150rpm and 30°C, ferment and cultivate in a 500L fermenter for 72 hours.

[0065] Step c, mixing Pseudomonas marine fermented liquid and EM bacterium fermented liquid, inoculate according to the inoculum size of 2% v / w (the inoculation ratio of marine Pseudomonas fermented liquid and EM bacterium fermented liquid is 1:1.5 ) into the bait (bait formula 1), adjust the moisture content to 42%. After mixing evenly, pack into 25kg plastic bags with a one-way air valve for anaerobic fermentation at 25°C for 7 days.

[0066] ...

Embodiment 2

[0076] Step a, marine pseudoalteromonas culture:

[0077] The fermentation broth of Pseudomonas marine has been prepared above.

[0078] Step b, mixed EM bacterial culture:

[0079] Take the seed liquids of the four strains of Bacillus, photosynthetic bacteria, yeast and lactic acid bacteria prepared above, and inoculate them into the mixed EM bacteria fermentation medium with the inoculum of 0.4%, 0.6%, 0.4%, and 0.6% v / v respectively , under the conditions of 180rpm and 30°C, ferment and cultivate in a 500L fermenter for 80 hours.

[0080] Step c, mixing the Pseudomonas marine fermented liquid and the EM bacterium fermented liquid, inoculating according to the inoculum size of 2% v / w (the ratio of the Pseudomonas marine fermented liquid and the EM bacterium fermented liquid is 1:2) In the bait (bait formula 2), adjust the moisture content to 45%. After mixing evenly, pack into 25kg plastic bags with a one-way air valve for anaerobic fermentation at 30°C for 5 days.

[00...

Embodiment 3

[0090] Step a, marine pseudoalteromonas culture:

[0091] The fermentation broth of Pseudomonas marine has been prepared above.

[0092] Step b, mixed EM bacterial culture:

[0093] Take the seed liquids of the four strains of Bacillus, photosynthetic bacteria, yeast and lactic acid bacteria prepared above, and inoculate them into the mixed EM bacteria fermentation medium with the inoculation amounts of 0.6%, 0.9%, 0.6%, and 0.9% v / v respectively , under the condition of 180rpm and 30°C, fermented and cultivated in a 500L fermenter for 72 hours.

[0094] Step c, mix Pseudomonas marine and EM bacteria, inoculate according to the inoculation amount of 3% v / w (the ratio of Pseudomonas marine and EM bacteria is 1:2.5) in the bait (bait formula 3) , adjust the moisture content to 48%. After mixing evenly, pack into 25kg plastic bags with a one-way air valve for anaerobic fermentation at 35°C for 3 days.

[0095] Step d. When the fermented bait has water vapor, it is slightly wa...

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Abstract

The present invention discloses a preparation method of sea cucumber biologically fermented baits. Marina pseudoalteromonas and mixed effective microorganisms (EM) are inoculated into the baits to be subjected to anaerobic fermentation at 25-35 DEG C for 3-7 days. The mixed EM consists of bacillus, photosynthetic bacteria, yeasts and lactobacillus. The method comprises the step of conducting solid state fermentation on the stichopus japonicus baits via the microorganisms to prepare the fermented baits. Compared with the unfermented baits, the prepared baits are significantly increased in the attractiveness to the stichopus japonicus. Besides, the fermented baits are more comprehensive in nutrition and more conductive to the growth of the stichopus japonicus, improve the survival rates of the stichopus japonicus, improve the intestinal flora of the stichopus japonicus, and improve the digestion and absorption rates of the baits by the stichopus japonicus. At the same time, due to the addition of seaweed gel degrading bacteria, the fermented baits can effectively degrade kelp powder rich in the seaweed gel and difficult to digest by the stichopus japonicus in the baits, so that the proportion of the kelp powder in the baits can be increased to replace parts of expensive sargassum and the purposes of lowering enterprise costs is acheived.

Description

technical field [0001] The invention relates to a fermented bait, in particular to a preparation method of a mixed EM bacteria fermented sea cucumber bait containing marine pseudoalteromonas. Background technique [0002] The body wall of sea cucumber is rich in collagen and polysaccharides, and contains calcium, iron, manganese and other trace elements. It has high edible and medicinal value, and is one of the important marine economic animals in northern my country. With the rapid development of intensification, scale and automation of the sea cucumber farming industry, the limited natural bait can no longer meet the needs of the actual market. However, there is still a lack of systematic research on the nutrition and bait of sea cucumber. Insufficient bait nutrition hinders the rapid growth of sea cucumbers, and some baits also have hygiene problems, carrying pathogenic bacteria, causing diseases, and even death, causing huge economic losses. The bait eaten by sea cucum...

Claims

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Application Information

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IPC IPC(8): A23K50/80A23K10/18A23K10/30A23K10/37A23K10/22A23K20/163
CPCY02A40/818Y02P60/87
Inventor 王际辉肖珊王晗郭航张天任
Owner DONGGUAN UNIV OF TECH
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