Rice blast resistance gene Pi1 and application thereof

A resistance gene and rice blast resistance technology, applied in the field of genetic engineering, can solve problems such as the lack of effective resistance genes, unresolved problems, and the susceptibility of disease-resistant varieties, and achieve the effect of shortening the breeding time

Inactive Publication Date: 2010-12-08
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the diversity and variability of the rice blast fungus population, coupled with the lack of effective use of resistance genes, and the lack of sufficient understanding of the resistance mechanism, the problem of susceptibility of resistant varieties has not been solved, but due to effective The lack of resistance genes and the short life of disease-resistant varieties have become the most difficult problems for breeders

Method used

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  • Rice blast resistance gene Pi1 and application thereof
  • Rice blast resistance gene Pi1 and application thereof
  • Rice blast resistance gene Pi1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Resistance characteristics of resistance gene Pi1

[0038] First, in order to compare and clarify the resistance spectrum of the five alleles (Pi1, Pik-p, Pik, Pik-m, pi1) at the Pi1 locus, using 40), Hunan (HN, 40), Yunnan (YN, 43), Guizhou (GZ, 60), Sichuan (SC, 66), Jiangsu (JS, 72), Liaoning (LN, 108 ), Jilin (JL, 60), Heilongjiang (HLJ, 63), a total of 612 strains collected were resistant to the varieties held by the above five alleles (respectively C101LAC, K60, Kusabue, Tsuyuake and CO39) comparative analysis. The results showed that Pi1 showed high resistance to 8 rice blast fungus populations except Liaoning and Jilin. This shows that the gene can be used in the above regions. Rice varieties C101LAC and CO39 have been disclosed in the literature (Inukai et al. Allelism of blastresistance genes in near-isogenic lines of rice. Phytopathology, 1994, 84: 1278-1283). Other rice varieties have been disclosed in the literature (Wang et al. Characterizati...

Embodiment 2

[0039] Example 2 Location and Electron Physical Mapping of Rice Blast Resistance Gene Pi1

[0040] F 2 Population, inoculated with the strain CHL930 (Wang et al. Characterization of rice blast resistance genes in the Pik cluster and fine mapping of the Pik-p locus. Phytopathology, 2009, 99 :900-905). The results showed that the F 2 The segregation ratio of resistant plants and susceptible plants in the population was 3:1. It was inferred that the resistance of C101LAC was controlled by a pair of dominant genes. Therefore, a mapping population consisting of 230 individuals (equivalent to 460 gametophytes) was constructed.

[0041] The previous genetic analysis showed that the Pi1 gene and the Pik cluster gene are closely linked (Inukai et al.1994, Phytopathology, 84: 1278-1283), and the applicant's laboratory has carried out fine mapping of the Pik-m gene (Li et al. 2007, Molecular Breeding, 20: 179-188). Therefore, applicants performed linkage analysis using molecular ma...

Embodiment 3

[0043] Example 3 Annotation and sequence analysis of rice blast resistance gene Pi1 candidate gene

[0044] In order to determine the candidate gene of Pi1, the applicant used the reference sequence of Nipponbare, through three gene prediction software RiceGAAS (http: / / ricegaas.dna.affrc.go.jp), Gramene (http: / / 143.48.220.116 / resources / ) and Softberry's FGENESH (http: / / www.softberry.com) performed gene prediction and annotation analysis on the target gene region, and preliminarily determined that the candidate resistance gene of Pi1 is 6 nucleotide binding sites and leucine-rich Candidate genes (Pi1-1N, Pi1-2N, Pi1-3N, Pi1-4N, Pi1-5N, and Pi1-6N) of nucleotide binding site-leucine-rich repeat (NBS-LRR).

[0045] The presence / absence (P / A) analysis of 6 candidate genes based on gene-specific markers showed that the 4 candidate genes (Pi1-1N, Pi1-2N, Pi1- 3N and Pi1-4N) were absent in C101LAC. According to the information provided by Ashikawa et al. (2008, Genetics, 180: 2267...

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Abstract

The invention discloses clone and application of rice blast resistance gene Pi1. The rice blast resistance gene Pi1 comprises gene Pi1-1, or Pi1-2, or tandem sequence of the Pi1-1 and the Pi1-2, the nucleotide sequence of the Pi1-1 is shown as SEQIDNO:1, the nucleotide sequence of the Pi1-2 is shown as SEQIDNO:2, and the nucleotide sequence of the tandem sequence is shown as SEQIDNO:3. The two genes of Pi1-1 and Pi1-2 belong to members of CC-NBS-LRR resistance gene family and are constitutive expression genes. The rice blast resistance gene Pi1 can endue plants with specific disease resistance response to disease caused by rice blast fungus (Magnaporthe oryzae), and is suitable for the plants sensitive to pathogens. By connecting the Pi1 gene sequence with plants transformation carriers to be introduced to plant cells, genetically modified resistant varieties can be obtained so as to be applied to farm production.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to the cloning and application of rice blast resistance gene Pi1. Background technique [0002] Plants are often attacked by various pathogens during the growth process, and plants adopt various defense strategies to protect themselves and avoid being attacked by them. One of the most important defense mechanisms in plants is the ability to recognize the presence of obligate pathogenic microorganisms and initiate their own defense response system. Plant recognition of pathogenic bacteria is mediated by disease resistance genes. Therefore, the analysis and research on the structure of disease-resistant gene products is the basis for understanding the mechanism of plant disease resistance, and also has important guiding significance for the prevention and control of diseases. [0003] To date, more than 80 disease resistance genes have been isolated from plan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/63C12N15/11C07K14/415C12Q1/68A01H5/00
Inventor 潘庆华
Owner SOUTH CHINA AGRI UNIV
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