Rice blast resistance gene Pik and application thereof

A technology of resistance gene and rice blast, which is applied in the field of genetic engineering, can solve the problems of lack of effective resistance gene, unresolved, disease-resistant varieties, etc., and achieve the effect of shortening the breeding time

Inactive Publication Date: 2012-12-19
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the diversity and variability of the rice blast fungus population, coupled with the lack of effective use of resistance genes, and the lack of sufficient understanding of the resistance mechanism, the problem of susceptibility of resistant varieties has not been solved, but due to effective The lack of resistance genes and the short life of disease-resistant varieties have become the most difficult problems for breeders

Method used

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  • Rice blast resistance gene Pik and application thereof
  • Rice blast resistance gene Pik and application thereof
  • Rice blast resistance gene Pik and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1: Resistance characteristics of the resistance gene Pik

[0052] First, in order to compare and clarify the resistance spectrum of the four alleles (Pik, Pik-p, Pik-s, Pik-m) at the Pik locus, using 40), Hunan (HN, 40), Guizhou (GZ, 60), Yunnan (YN, 43), Sichuan (SC, 66), Jiangsu (JS, 72), Liaoning (LN, 108 ), Jilin (JL, 60) and Heilongjiang (HLJ, 63), a total of 612 strains collected were compared to the varieties held by the above four alleles (respectively Kusabue, K60, Shin 2 and Tsuyuake) analyze. The results showed that Pik showed high resistance to the blast fungus populations in Guangdong, Fujian, Hunan, Guizhou, Sichuan and Jiangsu. This shows that the gene can be used in the above regions. Biomaterial rice varieties Kusabue, Shin 2, K60, and Tsuyuake have been reported in the literature (Wang et al. Characterization of riceblast resistance genes in the Pik cluster and fine mapping of the Pik-p locus. Phytopathology, 2009, 99: 900-905) public.

Embodiment 2

[0053] Example 2: Location and Electron Physical Mapping of Rice Blast Resistance Gene Pik

[0054] F 2 Populations were inoculated with strains such as CHL346 that exhibited distinct non-affinity / affinity responses to parental varieties (strains isolated from single spores of Magnaporthe grisea). The results showed that these F 2 The segregation ratio of resistant plants and susceptible plants in the population was 3:1. It was inferred that the resistance of Kusabue to these inoculated strains was controlled by a pair of dominant genes. to these F 2 Linkage analysis of the resistance genes corresponding to the population showed that they were controlled by the same dominant gene. Therefore, a mapping population consisting of 499 individuals (equivalent to 998 gametophytes) was constructed. The biomaterial rice variety IR36 has been disclosed in literature (Lin et al. A high-resolution map of the rice blast resistance gene Pi15 constructed by sequence-ready markers. Plant ...

Embodiment 3

[0057] Example 3: Annotation and sequence analysis of rice blast resistance gene Pik candidate gene

[0058] In order to determine the candidate gene of Pik, the applicant used the reference sequence of Nipponbare, through three gene prediction software RiceGAAS (http: / / ricegaas.dna.affrc.go.jp), Gramene (http: / / 143.48.220.116 / resources / ) and Softberry's FGENESH (http: / / www.softberry.com) performed gene prediction and annotation analysis on the target gene region, and initially determined that the candidate resistance gene of Pik is 6 nucleotide binding sites and leucine-rich Candidate genes (Pik1-NP, Pik2-NP, Pik3-NP, Pik4-NP, Pik5-NP, and Pik6-NP) of nucleotide binding site-leucine-rich repeat (NBS-LRR).

[0059] The presence / absence (P / A) analysis of 6 candidate genes based on gene-specific markers showed that the 4 candidate genes (Pik1-NP, Pik2-NP, Pik3- NP, Pik4-NP) are absent in Kusabue. Therefore, the candidate resistance genes of Pik actually only have 2 Pik-1 and ...

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Abstract

The invention discloses a rice blast resistance gene Pik and application thereof. The invention provides a nucleotide sequence of the rice blast resistance gene Pik and an amino acid polypeptide sequence coded by the nucleotide sequence. The nucleotide sequence comprises two genes which belong to members of a CC-NBS-LRR (CC Nucleotide Binding Site-Leucine-Rich Repeats) resistance gene family and are genes of constitutive expression. The invention also provides application to transforming rice or cultivating disease-resistant variety of other plants by applying the gene and breeding according to the molecular markers generated by the gene sequence.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to the cloning and application of rice blast resistance gene Pik. Background technique [0002] Plants are often attacked by various pathogens during the growth process, and plants adopt various defense strategies to protect themselves and avoid being attacked by them. One of the most important defense mechanisms in plants is the ability to recognize the presence of obligate pathogenic microorganisms and initiate their own defense response system. Plant recognition of pathogenic bacteria is mediated by disease resistance genes. Therefore, the analysis and research on the structure of disease-resistant gene products is the basis for understanding the mechanism of plant disease resistance, and also has important guiding significance for the prevention and control of diseases. [0003] To date, more than 80 disease resistance genes have been isolated from plan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/54C12N9/12C12N15/63C12N15/82A01P3/00C12Q1/68A01H1/04
Inventor 潘庆华翟纯林菲董忠秋王玲
Owner SOUTH CHINA AGRI UNIV
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