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Method for creating beet salt-resisting and anti-draught new seeds using stem-tip growth-point genetic conversion

A technology of genetic transformation and growth point, applied in the field of creating new salt- and drought-tolerant germplasm of sugar beet, can solve problems such as genetic transformation work that has not been reported, and achieve the effect of high multiplier multiples

Inactive Publication Date: 2003-03-26
SHANDONG UNIV
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AI Technical Summary

Problems solved by technology

So far, there has been no report on the genetic transformation of sugar beet growing point as the receptor

Method used

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  • Method for creating beet salt-resisting and anti-draught new seeds using stem-tip growth-point genetic conversion
  • Method for creating beet salt-resisting and anti-draught new seeds using stem-tip growth-point genetic conversion
  • Method for creating beet salt-resisting and anti-draught new seeds using stem-tip growth-point genetic conversion

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Experimental program
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Embodiment 1

[0044] 1. Materials and methods The bacterial strain is Agrobacterium tumefaciens LBA4404, which contains the pCAMBIA1300 plasmid carrying the target gene. The plasmid carries the target gene choline dehydrogenase (CDH) gene betA or NHX1 gene and constitutive 35S promoter, hygromycin phosphotransferase (HPT) gene, etc. ( figure 1 ). Choline dehydrogenase (CDH) gene betA is derived from E.coli, and the encoded CDH has the functions of choline monooxidase (CMO) and betaine aldehyde dehydrogenase (BADH), so single enzyme metabolic engineering can endow The ability of plants to accumulate glycine betaine. The NHX1 gene originates from Arabidopsis thaliana and encodes Na mainly distributed on the tonoplast + / H + antiporter [1] . (see schematic diagram figure 1 )

[0045] LBA4404 monoclonal culture was shaken overnight at 28°C in YEB liquid medium, centrifuged at 4000rpm for 5min, resuspended in sugar beet cluster shoot liquid medium, and diluted to OD 600 About 0.1-0.5 for...

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Abstract

The method for breeding salt-resistant drought-resistant new variety of sugar beet by utilizing shoot tip growing point genetic transformation includes the following main steps: using shoot tip growing point or tufted bud growing point of the sugar beet as receptor, using agrobacterium containing plant transformation carrier system to infect beet cell to produce transgenic plant, detecting exogenous gene and its expression strength and adverse-resistance of plant of setect out the salt-resistant drought-resistance transgenic plant. Said system uses the teneral inflorescence section of alabastrum stage of beet as explant, includes the tufted bud to produce and make mass proliferation on proper culture medium, strips foliode and petiolute to denude growing point for agrobacterium infection.

Description

(1) Technical field [0001] The invention belongs to the field of bioengineering breeding of crops or the field of crop breeding. Specifically, it relates to a method for creating new sugar beet salt- and drought-tolerant germplasm by genetically transforming shoot apex growth points. (2) Background technology [0002] Sugar beet (Beta) is an important sugar and feed crop. Sugar beet provides more than 40% of the world's sugar. Forage beet has large roots and rich nutrition. It is the best juicy feed for livestock and has a large biomass. Sugar beet has the potential of salt tolerance and can grow normally in soil with a salt content below 0.4%. Some wild relatives of cultivated sugar beet originated from coastal saline-alkali land and have strong salt tolerance. American scholars found that sugar beet can be irrigated with fresh seawater in the later stage of vegetative growth, but the salt tolerance of fodder beet is slightly poor. The development of animal husbandry and...

Claims

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Application Information

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IPC IPC(8): A01H1/00
Inventor 张举仁杨爱芳张可炜王海峰
Owner SHANDONG UNIV
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