Culture medium and method for culturing DCs

Abstract

The invention belongs to the field of cytobiology and particularly relates to a culture medium and method for culturing DCs. The culture medium for culturing the DCs comprises a first component, a second component and a third component. The first component is an RPMI1640 culture medium containing bovine serum albumin, interleukin 4, GM-CSF and stem cell factors (SCF). The second component is an RPMI1640 culture medium containing bovine serum albumin, interleukin 14, interleukin 2, interleukin 4 and GM-CSF. The third component is an RPMI1640 culture medium containing bovine serum albumin, CD40L, GM-CSF and interleukin 4. The result shows that the antigen presentation capacity of the DCs cultured by the culture medium is higher, the sensitization capacity to T cells is higher, and the killing capacity to tumors is greatly improved. Use of serum is avoided, and the risk of pathogenic microorganisms is relieved.

Description

technical field

[0001] The invention belongs to the field of cell biology, and in particular relates to a culture medium and a culture method for culturing DC cells. Background technique

[0002] DC cells are known to have the strongest function in vivo and the only professional antigen-presenting cells that can activate resting T cells. They are the central link in initiating, regulating and maintaining immune responses. By activating and culturing a large number of DC cells loaded with tumor antigens in vitro, when the number of cells reaches a certain number, they will be reinfused back to the patient, which can induce a strong anti-tumor immune response in the body. In the world, there are already tumor vaccines made by using DC cells, which present tumor cell antigens to T cells through the antigen presentation ability of DC cells, so that T cells can kill tumors in a targeted manner. However, the content of DC cells in the blood is not high, so obtaining a large numbe...

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