Method for promoting polysaccharide accumulation of hairy roots of beautiful millettia roots

A hairy root and Niu Dali technology, which is applied in the field of Niu Dali tissue culture, can solve problems such as taking a long time, degeneration, and slow development speed, and achieve the effects of facilitating development and utilization, enhancing synthesis ability, and accelerating growth speed

Inactive Publication Date: 2017-04-26
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, with the continuous increase in the demand for cattle, wild resources have been exhausted, and the supply of raw materials is seriously in short supply.
In order to solve the contradiction between supply and demand, people try to expand the drug source by artificial cultivation. Some successful research reports have been found so far, but there are still many problems restricting its large-scale production. It is very unstable, the survival rate of cutting seedlings is low, and it is very difficult for tissue culture seedlings to take root, etc.
Moreover, the growth cycle of cattle is long (generally 5 years before the production is formed), and it takes a long time to breed or raise seedlings by conventional methods. Due to the small reproduction coefficient and large seed consumption, the development speed is very slow and the production cost increases. The damage of plant diseases and insect pests seriously leads to degradation, which seriously affects the yield and quality

Method used

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  • Method for promoting polysaccharide accumulation of hairy roots of beautiful millettia roots
  • Method for promoting polysaccharide accumulation of hairy roots of beautiful millettia roots

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for promoting the accumulation of polysaccharides in bovine vigorous hairy roots, inoculating bovine vigorous hairy roots into a liquid medium and placing it on a constant temperature shaker for proliferation and culture for at least 30 days, and the liquid medium contains 100 μmol / L jasmonate formazan ester.

[0027] Wherein, the formula of the liquid medium also contains: 1 / 2MS, 0.5mg / L indolebutyric acid and 30g / L sucrose.

[0028] Wherein, the conditions for the proliferation culture are: light intensity 1500Lx, light time 10h / d.

[0029] Wherein, the rotation speed of the constant temperature shaker was 90 rpm, and the proliferation culture temperature was set at 24°C.

Embodiment 2

[0031] A method for promoting the accumulation of polysaccharides in bovine vigorous hairy roots, inoculating bovine vigorous hairy roots into a liquid medium and placing it on a constant temperature shaker for proliferation and culture for at least 30 days, and the liquid medium contains 300 μmol / L jasmonate formazan ester.

[0032] Wherein, the formula of the liquid medium also contains: 1 / 2MS, 1.0mg / L indolebutyric acid and 30g / L sucrose.

[0033] Wherein, the conditions of the proliferation culture are: light intensity 2000Lx, light time 10h / d.

[0034] Wherein, the rotation speed of the constant temperature shaker was 150 rpm, and the proliferation culture temperature was set at 28°C.

Embodiment 3

[0036] A method for promoting the accumulation of polysaccharides in bovine villous roots, inoculating bovine villous roots into a liquid medium and placing them on a constant temperature shaker for proliferation and culture for 30 days, and the liquid medium contains 220 μmol / L methyl jasmonate .

[0037] Wherein, the formula of the liquid medium also contains: 1 / 2MS, 0.8mg / L indolebutyric acid and 30g / L sucrose.

[0038] Wherein, the conditions for the proliferation culture are: light intensity 18000Lx, light time 10h / d.

[0039] Wherein, the rotation speed of the constant temperature shaker was 120rmp, and the proliferation culture temperature was set at 26°C.

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Abstract

The invention discloses a method for promoting the polysaccharide accumulation of hairy roots of beautiful millettia roots. The method comprises: inoculating hairy roots of beautiful millettia roots into a culture medium, and carrying out multiplication culture on a constant-temperature shaking table for at least 30 days, wherein the liquid culture medium contains 100-300mu m mol/L methyl jasmonate. According to the method for promoting the polysaccharide accumulation of hairy roots of beautiful millettia roots, disclosed by the invention, the hairy roots of the beautiful millettia roots are subjected to amplification culture under a specific condition, so that the content of beautiful millettia root polysaccharide in the hairy roots of the beautiful millettia roots is increased, and a new approach is provided for obtaining beautiful millettia root polysaccharide.

Description

technical field [0001] The invention relates to the technical field of bovine vigorous tissue culture. More specifically, the present invention relates to a method for promoting the accumulation of polysaccharides in the hairy root of bovine herpes. Background technique [0002] Niu Dali is a plant of Millettia speciosa Champ. in the leguminous family. Modern research has found that Niu Dali contains polysaccharides, alkaloids, coumarins and various trace elements, among which Niu Dali polysaccharide is its The main active ingredient has the functions of regulating immune system, anti-oxidation, anti-inflammation, and anti-tumor, especially in anti-tumor. Current clinical studies have shown that it has good therapeutic effects on nasopharyngeal cancer, ovarian cancer, lung cancer, and colon cancer , at the same time, bovine herbaceous polysaccharide is also an ideal immune enhancer, and its demand in drug preparation is increasing day by day. [0003] However, with the con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 姚绍嫦白隆华蓝祖栽翟勇进黄浩韦荣昌
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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