The invention discloses a seedling transplanting method for seedling seeds of canna edulis Ker. The method comprises steps including seed treatment, sowing, greenhouse culture management, transplantedseedling matrix preparation, seedling transplantation and management, seedling hardening, transplantation to a large field and the like. The seedling seeds of canna edulis Ker are comprehensively treated through proper machinery, agents and temperatures, the dormancy period of the seedling seeds can be broken, rapid and regular germination of the seeds can be promoted, and the germination rate reaches 90% or above; besides, a transplanted seedling matrix is adjusted, conditions such as temperature, humidity and the like of seedlings are strictly controlled, the seeds can germinate regularly within 5-7 d, consistent growth of seedlings is guaranteed, the whole process is time-saving and labor-saving, the seedling culture time is flexible, the crossbreeding efficiency of the canna edulis Ker is improved, and the breeding time of good varieties is shortened.