57results about How to "Consistent growth" patented technology

An MRAM structure is disclosed in which the bottom electrode has an amorphous TaN capping layer to consistently provide smooth and dense growth for AFM, pinned, tunnel barrier, and free layers in an overlying MTJ. Unlike a conventional Ta capping layer, TaN is oxidation resistant and has high resistivity to avoid shunting of a sense current caused by redeposition of the capping layer on the sidewalls of the tunnel barrier layer. Alternatively, the α-TaN layer is the seed layer in the MTJ. Furthermore, the seed layer may be a composite layer comprised of a NiCr, NiFe, or NiFeCr layer on the α-TaN layer. An α-TaN capping layer or seed layer can also be used in a TMR read head. An MTJ formed on an α-TaN capping layer has a high MR ratio, high Vb, and a RA similar to results obtained from MTJs based on an optimized Ta capping layer.

A small percentage of cells within an established solid tumor have the properties of stem cells. These solid tumor stem cells give rise to both more tumor stem cells and to the majority of cells in the tumor that have lost the capacity for extensive proliferation and the ability to give rise to new tumors. Thus, solid tumor heterogeneity reflects the presence of tumor cell progeny arising from a solid tumor stem cell.We have developed a xenograft model in which we have been able to establish tumors from primary tumors via injection of tumors in the mammary gland of severely immunodeficient mice. These xenograft assay have allowed us to do biological and molecular assays to characterize clonogenic solid tumor stem cells.We have also developed evidence that strongly implicates the Notch pathway, especially Notch 4, as playing a central pathway in carcinogenesis.

The invention belongs to the field of microalgae culture, and in particular relates to a half-dry solid-state adherent culture device for microalgae industrial production. The half-dry solid-state adherent culture device comprises a bracket system, adherent culture units, a supporting shaft transmission system, a speed regulating motor, liquid supplementation pipes, collection grooves and a culture medium liquid storage tank, wherein at least one adherent culture unit is arranged on the bracket system and is connected with the speed regulating motor through the supporting shaft transmission system; the liquid supplementation pipes are respectively arranged above the adherent culture unit and are communicated with the culture medium liquid storage tank through pipelines; the collection grooves are respectively formed below the adherent culture unit and are communicated with the culture medium liquid storage tank through liquid collection pipes; a liquid circulating pump and a carbon supplementation device are arranged in the culture medium liquid storage tank. The half-dry solid-state adherent culture device can be used for producing microalgae biomass and secondary metabolites on a large scale; the yield in unit floor area is effectively improved; the culture period is shortened; the culture water consumption is greatly reduced; the cost of the culture device and the operation cost are reduced; the industrial magnification is facilitated.

The invention discloses a breeding method of bactrocera cucurbitae, which comprises the steps of egg collection, pupa collection, pupa eclosion and imago breeding. According to the method, a natural-growth environmental condition and a living habit of the Bactrocera cucurbitae are researched and analyzed, so that a successful method for artificially breeding the Bactrocera cucurbitae is obtained by creative exploration, and the Bactrocera cucurbitae with basically-consistent growth and development conditions can be obtained in batches; on the basis of the method, the bactrocera cucurbitae meeting a using requirement of scientific experiment research can be provided for scientific experiments all the year around without limitation of natural conditions and seasons; and the breeding method can increase a survival rate of artificial breeding of the Bactrocera cucurbitae, and improve the breeding efficiency.

The sputtering apparatus includes a vacuum vessel, a sputter electrode placed within the vacuum vessel to hold a target material to be sputtered, a radio frequency power source for applying radio frequency waves to the electrode, a substrate holder which is spaced from the electrode and on which a substrate is held, a thin film being to be deposited on the substrate from components of the target material, and an impedance adjusting circuit for adjusting a first impedance of the substrate holder. The impedance adjusting circuit has a first end directly set at a ground potential and has an impedance circuit which is adjustable for adjusting the first impedance, a second impedance of the impedance circuit is adjusted to thereby adjust the first impedance and, hence, a potential of the substrate.

The present invention relates to a chemical vapor deposition device comprising a Raman spectroscopy in-situ measurement cavity. The device comprises a transmission device, a reaction cavity for performing chemical vapor deposition reaction on a thin film material and a monitoring cavity for performing Raman spectroscopy in-situ monitoring on the thin film material; the reaction cavity communicateswith the monitoring cavity, a reaction base is arranged in the reaction cavity, and a Raman spectroscopy monitoring module is arranged in the monitoring cavity; the reaction base is used as a substrate for film material growth; the transmission device is used for controlling the reaction base to move between the reaction chamber and the monitoring chamber; and the Raman spectroscopy monitoring module is used for performing Raman spectroscopy in-situ monitoring on the thin film material moved into the monitoring cavity. The present invention provides the chemical vapor deposition device comprising the Raman spectroscopy in-situ measurement cavity, which solves the problem of in-situ Raman detection of the thin film under the condition that the growth of the thin film is not affected, and further can adjust the process in real time to reduce defects.

The invention relates to a drought-tolerance planting method. The method includes the three steps of nursery building, seedling raising and transplanting. By reasonably planning a nursery, reasonably processing seeds or cottage branches and reasonably distributing transplanting soil, the survival rate of tree seeds is increased, diseases and pests are reduced, it can be ensured that tree seed growth spaces and conditions in the same area are basically consistent, and the tree seeds uniformly grow.

The invention discloses a seedling culturing method for keeping consistency of the seedling age of cabbage seedlings in a same plug, belonging to the technical field of vegetable culturing. The method comprises the following steps: selecting a smooth land parcel in a greenhouse, evenly laying special matrixes with the thickness of about 3cm, pouring water through the matrixes, evenly spreading seeds, scattering a layer of matrixes with the thickness of 0.3cm on the spread seeds, pouring a small amount of water, and waiting for seedling emergence; placing the matrixes in an empty plug, pouring water on the matrixes, transferring the batch of seedlings to the pre-prepared plug after the leaves of the cabbage seedlings emerging at first are open and flat, and ensuring a seedling in a hole; transferring the plug which is filled with the seedlings to the greenhouse for centralized management; and repeatedly carrying out the steps until the seedlings do not exist in a seedbed. In the invention, the seedlings with the consistent seedling age, even size and consistent growth situation are transferred into the same plug, and therefore, as long as the seedlings in the seed bed are transferred into the same plug in accordance with the method, the seedlings in the same plug are ensured to be consistent in the later growth.

The invention discloses a seedling-adhering method for porphyra haitanensis conchospores. The method comprises the following steps: setting up a conchospore spreading pond, a conchospore collecting pond and a conchospore seedling-adhering pond, wherein the conchospore collecting pond is located at a higher position, and the conchospore seedling-adhering pond is located at a lower position; uniformly spraying seawater containing conchospores in the conchospore collecting pond onto a seedling curtain in the conchospore seedling-adhering pond by using potential energy difference through a porphyra haitanensis conchospore water-spray seedling-adhering device. The porphyra haitanensis conchospore water-spray seedling-adhering device is used, and therefore the method disclosed by the invention has the advantages that the densities and the spore growth statuses of the same batch are uniform, the seedling-adhering conditions are improved, the yield and the production value are favorably ensured, and the management intensity of seedling raising is lightened.

The invention discloses a method for preparing conidia of lasiodiplodia theobromae. The method comprises the following steps: 1) inoculating lasiodiplodia theobromae onto a PDA (potato dextrose agar) culture medium to be cultured until a colony grows vigorously; 2) selecting a grape variety sensitive to lasiodiplodia theobromae, cutting healthy green grape branches into 30cm sections and sterilizing the surfaces of the sections; 3) punching holes in the green branches and removing the phloem; preparing hypha blocks from the colony with a sterilized puncher, inoculating the prepared hypha blocks in the holes, wrapping the hypha blocks with films to preserve moisture and inserting the inoculated branches in tissue culture containers filled with sterile water to be cultured at 28 DEG C; culturing for 48 hours under the condition that the relative humidity is more than 90%, and then maintaining the humidity at 70% and culturing for 5-10 days. The method has the beneficial effects that the preparation time of conidia can be shortened; prepared spore suspension has small spore difference, stable quality and strong pathogenicity and provides guarantees for pathogenicity identification, population genetic differentiation, pathogenesis analysis, and the like of lasiodiplodia theobromae.

The invention provides a tissue culture method of Stachys siebolidii Miq. According to the method, a propagation system of commercial test-tube seedlings is obtained through the steps of induction, propagation expanding, seedling strengthening, rooting and the like of Stachys siebolidii Miq immature stem segments in an in-vitro condition, thereby shortening the propagation cycle, increasing the proliferation rate, making the seedlings consistent in grow state, excellent in quality and not restricted by seasons, achieving anniversary production, and achieving the propagation of high-quality commercial Stachys siebolidii Miq seedlings through a tissue culture mode.

The invention discloses a method for walnut seedling culture and transplantation. Through seedling setting with trays, root cutting transplanting and container culture, the growth process and the transplanting process of seedlings do not affect each other. Soil balls which seedling roots take greatly reduce loss of roots in the transplanting process, the transplanting survival rate is improved, the rejuvenation period is shortened, root systems of the cultured seedlings are flourishing, and the standard of grafting on the same year is met. The method is simple to operate, practical, low in cost and consistent in growth vigor, the transplanting survival rate is 95% or above, the seedling growth amount is obvious on the year of transplantation, nearly a half of rootstocks can reach the grafting standard when autumn is up, the seedling culture time is short, and the method is suitable for large-scale reproduction.

The invention discloses a seedling transplanting method for seedling seeds of canna edulis Ker. The method comprises steps including seed treatment, sowing, greenhouse culture management, transplantedseedling matrix preparation, seedling transplantation and management, seedling hardening, transplantation to a large field and the like. The seedling seeds of canna edulis Ker are comprehensively treated through proper machinery, agents and temperatures, the dormancy period of the seedling seeds can be broken, rapid and regular germination of the seeds can be promoted, and the germination rate reaches 90% or above; besides, a transplanted seedling matrix is adjusted, conditions such as temperature, humidity and the like of seedlings are strictly controlled, the seeds can germinate regularly within 5-7 d, consistent growth of seedlings is guaranteed, the whole process is time-saving and labor-saving, the seedling culture time is flexible, the crossbreeding efficiency of the canna edulis Ker is improved, and the breeding time of good varieties is shortened.

The invention relates to the technical field of bletilla striata seedling raising, and discloses a rapid seedling raising device for bletilla striata. The device comprises a seedling raising disc, a supporting base is arranged below the seedling raising disc, a supporting ring is fixedly connected to the annular side wall of the seedling raising disc, supporting legs are symmetrically and fixedlyconnected to the lower end of the supporting ring, and the other ends of the supporting legs are fixedly connected to the upper end of the supporting base. A seedling jacking mechanism is connected between the seedling raising disc and the supporting base, and a light supplementing mechanism is connected to the center of the upper end of the seedling raising disc. The seedling jacking mechanism comprises a driving motor, the driving motor is fixedly connected to the center of the upper end of the supporting base, the output end of the driving motor is connected with a threaded rod through a coupler, the threaded rod is in threaded connection with a threaded pipe, and the upper end of the threaded pipe is fixedly connected with a supporting plate. According to the rapid seedling raising device for the bletilla striata, the problems that when cultivated bletilla striata seedlings are transplanted at present, soil around the bletilla striata seedlings is loosened, so that the root systemsof the bletilla striata seedlings are damaged, and the survival rate of the transplanted bletilla striata seedlings is reduced can be solved.

The invention belongs to the field of microalgae culture and specifically relates to a multilayer semi-dry adherent culture device provided with multilayer adherent culture units. The adherent culture units are arranged horizontally or arranged forming a certain inclination angle from the horizontal plane, and one end of each multilayer adherent culture unit is aligned and the multilayer adherent culture units are parallelly arranged on a bracket system. Lengths of the multilayer adherent culture units are progressively increased successively from top to bottom, and the multilayer adherent culture units are driven by a motor to rotate. During operation of the device, a microalgae seed solution is firstly sprayed through a microalgae inoculators onto each layer of the adherent culture unit successively; a medium drips onto culture surfaces of all the adherent culture units through a medium distributor, and the surplus liquid returns back to a medium collecting tank under the action of gravity so as to be repeatedly used; and when an algae mud scraper is used for harvesting algae mud, the blade sticks close to the surfaces of the adherent culture units, and algae mud is scrapped off into an algae mud collecting tank with rotating of the adherent culture units. According to the invention, the problem of uneven light distribution is solved, the device is convenient for inoculation and harvesting, and efficient utilization of luminous energy is realized.

The invention provides a wild buckwheat seedling breeding method. The wild buckwheat seedling breeding method comprises the following steps of (1) selecting wild buckwheat stalks to prepare cutting stalks; (2) soaking one ends of the cutting stalks in a rooting agent for a certain period of time, and then taking out the cutting stalks; (3) inserting one ends of the cutting stalks soaked with the rooting agent into a cutting garden; and (4) after cutting is completed, arranging a sun-shading part above the cutting garden, and breeding wild buckwheat seedlings. Seedlings bred by the wild buckwheat seedling breeding method provided by the invention have the advantages that the transplanting survival rate is up to 96 percent, and the growth condition is good. Meanwhile, the wild buckwheat seedlings bred through the breeding method can avoid the problems that the provenance degradation rate of seedlings produced through tuberous root breeding is high, the infection rate is high in the subsequent growth process, the provenance tuberous root breeding coefficient is low, and the use amount of tuberous root seedlings is large.

The invention discloses a planting method for salviae miltiorrhizae. The method comprises the following steps that surface dressing is conducted, the section achieving ventilation and lighting is selected, after deep ploughing is conducted, planting hole are dug after soil leveling is conducted, watering is conducted thoroughly, and a seedbed is formed; germination accelerating and seedling raising are conducted, seeds are soaked with clear water, then the seeds are wrapped by wet cloth and placed in a thermostat till most of the seeds sprout, and then the seeds are subjected to spot sowing onthe seedbed; seedling transplanting and field management are conducted finally, wherein developed seedlings are transplanted to standard planting holes in a field, complementary planting of seedlingsis conducted within one month after the seedlings are transplanted, then intertillage is conducted 3-5 times, and watering and fertilization are conducted in sequence every 15-20 days; pests and thelike can be effectively prevented, and by means of germination accelerating and seedling raising, the growth vigour can be consistent; standard single moisture planting is adopted, development and planting of the salviae miltiorrhizae are facilitated; by means of a few mount of promoting type field maintenance, excessive unbalanced growth of the salviae miltiorrhizae can be avoided, growth of roots of the salviae miltiorrhizae can be facilitated, and the planting benefit is increased.

The invention discloses a mobile weighing crop water demand observation system, which is characterized in that it comprises a measuring cylinder (1), a movable truss (2), a weighing device (3), a rainproof shed (4) and a measuring cylinder area (5); the measuring cylinder (1) is multiple, and each measuring cylinder (1) is installed in the measuring cylinder area (5) in the way of buried double sleeves, and the movable truss (2) is erected on the Above the measuring cylinder area (5), the weighing device (3) is installed on the movable truss (2) through a pulley and can be moved to the top of each measuring cylinder (1) to lift and weigh it. The rainproof The shed (4) is erected on the track dedicated to the long side direction of the measuring cylinder area (5), which can avoid the influence of wind and precipitation on the observation. The invention can observe the water demand of crops with an accuracy of 0.03-0.1mm in the change of water layer, can meet the strict requirements of irrigation tests on precision, and has the characteristics of low cost and small occupied area, and is suitable for popularization in agricultural irrigation tests.

The invention discloses a broad-spectrum anti-phage E. Coli BL21-(DE3)-PR and application thereof. A recombinant protein expression strain E. coli BL21(DE3)-PR is successfully screened with an engineering strain E. coli E. coli BL21(DE3) as a parent strain. The strain is resistant to infection of a phage capable of splitting E. coli BL21 (DE3). The broad-spectrum anti-phage E. Coli BL21-(DE3)-PR performs growth curve and protein expression assay on the recombinant protein expression strain E. coli BL21(DE3)-PR, and results indicate that the growth capability and protein expression capability of the stain are basically the same as an original strain E. coli BL21 (DE3).

The invention belongs to the field of microalgae culture and specifically relates to a multilayer semi-dry adherent culture device provided with multilayer adherent culture units. The adherent culture units are arranged horizontally or arranged forming a certain inclination angle from the horizontal plane, and one end of each multilayer adherent culture unit is aligned and the multilayer adherent culture units are parallelly arranged on a bracket system. Lengths of the multilayer adherent culture units are progressively increased successively from top to bottom, and the multilayer adherent culture units are driven by a motor to rotate. During operation of the device, a microalgae seed solution is firstly sprayed through a microalgae inoculators onto each layer of the adherent culture unit successively; a medium drips onto culture surfaces of all the adherent culture units through a medium distributor, and the surplus liquid returns back to a medium collecting tank under the action of gravity so as to be repeatedly used; and when an algae mud scraper is used for harvesting algae mud, the blade sticks close to the surfaces of the adherent culture units, and algae mud is scrapped off into an algae mud collecting tank with rotating of the adherent culture units. According to the invention, the problem of uneven light distribution is solved, the device is convenient for inoculation and harvesting, and efficient utilization of luminous energy is realized.

The invention belongs to the field of microalgae culture, and in particular relates to a half-dry solid-state adherent culture device for microalgae industrial production. The half-dry solid-state adherent culture device comprises a bracket system, adherent culture units, a supporting shaft transmission system, a speed regulating motor, liquid supplementation pipes, collection grooves and a culture medium liquid storage tank, wherein at least one adherent culture unit is arranged on the bracket system and is connected with the speed regulating motor through the supporting shaft transmission system; the liquid supplementation pipes are respectively arranged above the adherent culture unit and are communicated with the culture medium liquid storage tank through pipelines; the collection grooves are respectively formed below the adherent culture unit and are communicated with the culture medium liquid storage tank through liquid collection pipes; a liquid circulating pump and a carbon supplementation device are arranged in the culture medium liquid storage tank. The half-dry solid-state adherent culture device can be used for producing microalgae biomass and secondary metabolites on a large scale; the yield in unit floor area is effectively improved; the culture period is shortened; the culture water consumption is greatly reduced; the cost of the culture device and the operation cost are reduced; the industrial magnification is facilitated.

The invention discloses broad-spectrum antiphage E.coli BL21(DE3)-PR and application thereof. The engineering strain escherichia coli E.coli BL21(DE3)-PR is used as a parent strain to successfully screen one recombinant protein expression strain escherichia coli E.coli BL21(DE3)-PR; and the strain can be used for resisting infection of a phage capable of cracking the escherichia coli E.coli BL21(DE3)-PR. The recombinant protein expression strain escherichia coli E.coli BL21(DE3)-PR is subjected to growth curve and protein expression determination; and a result shows that the growth capabilityand protein expression capability of the strain are basically the same as those of an original strain E.coli BL21(DE3).

The invention discloses an automatic hermetia illucens box culture system which comprises a culture box frame, culture boxes, a camera, a slurry conveying unit, a larva feeding device and a control terminal. An automatic door is arranged at the bottom of each culture box, and a larva body conveying belt is arranged below each layer of culture box; the slurry conveying unit comprises a slurry pump and a slurry tank which are connected through a slurry conveying pipe, the slurry conveying pipe extends out to form a branch pipe with a slurry spraying hole above each layer of culture box, and an electric valve is arranged at each slurry spraying hole; and the control terminal is electrically connected with the camera, the automatic door, the larva body conveying belt, the slurry pump, the electric valves and the larva feeding device. According to the hermetia illucens box culture system, it can be guaranteed that the growth condition of larva bodies in each culture box reaches the standard, manual box drawing, box placing, feeding and discharging are not needed, it can be guaranteed that the growth condition of the larva bodies reaches the standard while the production efficiency is greatly improved, and finally the product quality is guaranteed.

The invention discloses a taxodium Zhongshanha container seedling and field integrated cultivation method, and belongs to the technical field of taxodium Zhongshanha cultivation. The method comprises the following steps: selecting 1-year-old taxodium zhongshanha cutting seedlings, after classification, planting the seedlings in a cutting bed temporarily in time, planting container seedlings while taking the container seedlings during field planting, and conducting watering in time after planting; performing deep ploughing on a clayed soil area before winter, performing leveling and bedding in early spring of the next year, and directly performing field improvement and bedding on a sandy soil area before operation in early spring; carrying out field ditching and shallow burying after the container seedlings are planted; performing spray irrigation on shallowly-buried container seedlings, manually pulling out weeds in the container in time after the seedlings survive, and pruning lateral branches and competitive branches in the growing period; and finally outplanting the seedlings in the form of container seedlings. Through two technical links of container planting and field shallow burying management and protection, the advantages of two cultivation methods of container seedling cultivation and field cultivation are combined, and the method has the advantages that cultivation conditions are controllable, the survival rate of seedlings after field planting is high, the growth vigor is consistent, and seedling outplanting is not limited by seasons.

The present invention relates to a method for synthesizing artificial seeds of spruce spruce. The artificial seed embryo adopts the somatic embryo induced by callus, and the somatic embryo is used as embedding after being dried, and the artificial endosperm includes 1 / 4LM, 0.5mg / L of IBA and 20g / L of sucrose, the artificial seed coat is made by immersing the artificial seed embryo and artificial endosperm into the artificial seed coat liquid and solidifying, and the artificial seed coat liquid includes the mass percentage concentration 2-3% sodium alginate and 1.5-2.5% CaCl by mass 2 . The sandy spruce artificial seed of the invention is not limited by seasons, and has better nutrient supply and disease resistance. The synthesis of artificial seeds of spruce sand will solve major problems such as low seed yield, shortage of seedlings, resource protection and maintenance of excellent traits of spruce sand, and it is also convenient for transportation, so that spruce sand can be better used in sandy land Ecological construction such as governance and construction of timber forests.

The invention provides a method for inducing plant regeneration and constructing a transgenic plant regeneration system for the whole plant of each part of Eucommia ulmoides, belonging to the technical field of woody plant tissue culture. The method for inducing plant regeneration by using the whole plant of each part of Eucommia ulmoides provided by the present invention includes the following steps: obtaining callus by induction of Eucommia seed embryos, stems and / or roots, and obtaining complete regeneration of Eucommia ulmoides through differentiated culture and rooting culture of the callus Plant; the medium of the differentiation culture is: basal medium+0.5-2mg / L6-BA+1.0-2.0mg / LIAA+25-35g / L sucrose+8-10g / L agar, pH is 5.8-6. The method for plant regeneration provided by the invention can be carried out through the roots, stems and seed embryos of Eucommia ulmoides, which has a wider application range, and the corresponding parts can be selected for regeneration according to needs, and the obtained tissue culture regeneration seedlings have corresponding Excellent traits, the cultivated tissue culture seedlings have a consistent and good growth state, are not easy to contaminate bacteria, are not easy to brown, and have a high differentiation rate and rooting rate.

The invention discloses a pepper planting method, which at least comprises a seedling raising step, and the seedling raising step comprises the following steps: step 1) cleaning and sterilizing; step 2) germination acceleration; and step 3) rejection: putting the pepper seeds subjected to germination acceleration into a vibrating screen, starting the vibrating screen, generating low-frequency vibration on the pepper seeds by using the vibrating screen, crushing relatively weak buds in the pepper seeds by relatively strong buds in the low-frequency vibration process, and further performing vibration rejection on the pepper seeds subjected to germination acceleration. According to the method, the vibration rejecting link is set in a seedling raising step, weak buds are rejected through vibration, the growth state of the planted pepper is consistent, pepper planting management is facilitated, a heat storage and heat preservation layer is arranged in germination accelerating equipment, steam heat in a steam cavity can be absorbed by the heat storage and heat preservation layer, the pepper seeds in a storage bin can be continuously subjected to heat preservation and heating, so that the germination accelerating efficiency of the pepper seeds is improved, and meanwhile, the energy consumption of steam generation equipment is reduced.