45 results about "Taxodium" patented technology

A pezicula sp. SC1337 strain, and a method of preparing trisaccharide ester derivatives by utilizing the strain are disclosed. The accession number of the pezicula sp. SC1337 is GDMCC No:60144. The pezicula sp. SC1337 is separated from endophytes of branches of taxodium distichum (L.) Rich., and can generate five compounds having obvious inhibiting activity on twelve common plant pathogenic fungi and having fresh-keeping effects on fruits, and therefore the strain can be used for preparing the five compounds having obvious inhibiting activity on the twelve common plant pathogenic fungi and having fresh-keeping effects on fruits, the compounds are used for control of the plant pathogenic fungi and fresh-keeping of fruits, and the strain has a wide application prospect.

The invention discloses a ThHAM1 gene related to development of roots of taxodium zhongshansha and application for the ThHAM1 gene and belongs to the technical field of plant gene engineering. A nucleotide sequence of the ThHAM1 gene is shown as SEQ ID NO. 1, and an amino acid sequence of an expression protein of the ThHAM1 gene is shown as SEQ ID NO. 3. Populus davidiana x P.bolleana is convertedby constructing a gene GbF3'H1 vector, compared with a control plant, the ThHAM1 gene performs efficient expression in the Populus davidiana x P.bolleana, following the situation that variation of aphenotype occurs, a growth speed of a plant is slowed with roots being most obvious, the number of adventitious roots is reduced, a length is shortened, and the expression quantity of a target gene isincreased by 200-500 times, so that illustrated that the ThHAM1 gene is an important gene for regulating and controlling development of the plant roots and influencing a plant morphology, and the ThHAM1 gene has an important application value in the field of forest tree gene engineering and can be used for regulating a plant growth and development speed or the plant morphology.

The present invention discloses a taxodium hybrid 'zhongshanshan' ThSHR3 gene for promoting plant growth and an application of the taxodium hybrid 'zhongshanshan' ThSHR3 gene, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the ThSHR3 gene is shown in SEQ ID NO.1 and an amino acid sequence of an expressed protein is shown in SEQ ID NO.2. Sequence alignment and evolution analysis of the ThSHR3 gene show that the ThSHR3 gene belongs to a SHR gene subfamily. Populus davidiana x P.bolleana is transformed by constructing a gene pH35GS-ThSHR3 vector. Compared with the control plant, the ThSHR3 gene is highly expressed in the Populus davidiana x P.bolleana, the expression amount of the target gene is increased by 500 times or more, and the plant growthrate is significantly higher than that of the control plants, indicating that the ThSHR3 gene is an important gene that regulates plant development and affects plant morphology, has important application value in the field of forest genetic engineering and can be used to adjust plant growth and development speed or plant morphology.

The invention relates to the technical field of ecological management, and provides a reservoir tail hydro-fluctuation belt wetland restoration method based on forest habitat. The method comprises the following steps of S1, selecting saplings: selecting a flooding-resistant large arbor with the height that the tree top can exceed the highest water level by 20-80cm after the tree top is submerged in the highest water level after planting, the large arbor comprises one or more of pond fir, Chinese tipping, taxodium zhongshanha and taxodium falcatum; and S2, planting, wherein planning small-sized trees resistant to water logging along a riparian zone of a reservoir tail hydro-fluctuation belt, and planning the large arbors below small arbors at a certain distance. According to the method, the spatial heterogeneity of the reservoir tail hydro-fluctuation belt is improved, and the original vegetation condition can be quickly and completely recovered after the reservoir tail hydro-fluctuation belt goes through a long-time highest water level environment.

The invention discloses an in-vitro quick propagating method of Taxodium.distichum, which pertains to the field of plant tissue culture. The method of the invention comprises the following steps: (1) taking mature embryos of sterilized Taxodium.distichum seeds as explant; (2) induction culture of adventitious buds; (3) elongation culture of adventitious buds; (4) propagation culture of adventitious buds; (5) culture of sound plantlets; (6) induction culture of adventitious roots; (7) formation of regenerative plants; (8) plantlets hardening and transplanting; wherein the basic culture medium applied to each culture stage is SH culture medium; the growth regulator added at each culture stage has the most suitable use amount and proportion which are selected by orthogonal design experiments. The test-tube plantlets of the Taxodium.distichum bred by the method of the invention have strong growth capacity, high propagation coefficient, up to 80% of the inducing rooting rate, approximate 90% of the transplant survival rate, and can be used for large-scale and industrialized production.

The invention relates to the technical field of pesticide synergists, and discloses a botanical synergist of emamectin benzoate. The synergist is prepared by mixing taxodium zhongshanha essential oil with emamectin benzoate according to a mass ratio of (10-50): 1 for use, and can be used as an insecticide for preventing and controlling spodoptera frugiperda. The synergist can remarkably improve the pesticide effect of the emamectin benzoate, can reduce the dosage of the emamectin benzoate under the same prevention effect, overcomes and delays the pesticide resistance of pests, and has the significance of safety, environmental protection, reduction of environmental pollution, remarkable economic benefits and environmental protection.

The invention discloses a taxodium Zhongshanha container seedling and field integrated cultivation method, and belongs to the technical field of taxodium Zhongshanha cultivation. The method comprises the following steps: selecting 1-year-old taxodium zhongshanha cutting seedlings, after classification, planting the seedlings in a cutting bed temporarily in time, planting container seedlings while taking the container seedlings during field planting, and conducting watering in time after planting; performing deep ploughing on a clayed soil area before winter, performing leveling and bedding in early spring of the next year, and directly performing field improvement and bedding on a sandy soil area before operation in early spring; carrying out field ditching and shallow burying after the container seedlings are planted; performing spray irrigation on shallowly-buried container seedlings, manually pulling out weeds in the container in time after the seedlings survive, and pruning lateral branches and competitive branches in the growing period; and finally outplanting the seedlings in the form of container seedlings. Through two technical links of container planting and field shallow burying management and protection, the advantages of two cultivation methods of container seedling cultivation and field cultivation are combined, and the method has the advantages that cultivation conditions are controllable, the survival rate of seedlings after field planting is high, the growth vigor is consistent, and seedling outplanting is not limited by seasons.

The invention provides a hydro-fluctuation belt biological treatment method. The method comprises steps of planting Taxodium hybrid 'Zhongshanshan' in areas having 10 meters or lower elevation difference with the highest elevation of the hydro-fluctuation belt when the hydro-fluctuation belt is exposed out of the water surface, hybrid-planting fodder mulberry, Bermuda grass or entada pstems between the Taxodium hybrid 'Zhongshanshan'. By the use of the biological treatment method, high plant survival rate can be achieved; and great and stable biological treatment effect can be realized.

The invention discloses an in-vitro propagation method of taxodium hybrids 'zhongshansha136'. The in-vitro propagation method includes the following steps that (1) explants are selected and disinfected; (2) a 1 / 2MS culture medium is improved; (3) the processed explants are cut, and stem primary induction culture and high-voltage electrostatic field (HVEF) processing are conducted; (4) secondary multiplication culture is conducted on adventitious buds obtained in the step (3); (5) root induction culture is conducted on rootless test-tube plantlets obtained in the step (4); (6) domestication seedling and transplanting are conducted on aseptic seedlings obtained in the step (5). The tissue culture propagation method is used for conducting seedling on the taxodium hybrids 'zhongshansha136', the seedling time is short, the reproduction rate is increased by 88-93 times, the rooting rate is 89%, the average root number is 4.1, the seedling survival rate reaches 95%, taxodium hybrids 'zhongshansha136' seedlings can be obtained efficiently, and the requirement for the taxodium hybrids 'zhongshansha136' seedlings of the market is met to a large extent.

The invention discloses a tissue culture propagation method of taxodium hybrids'zhongshansha118'. The method comprises the following steps: (1) selecting and disinfecting an explant; (2) improving a 1 / 2 MS culture medium; (3) cutting the treated explant, carrying out stem primary induction culture and treating in a high-voltage electrostatic field (HVEF); (4) carrying out subculture multiplication culture on adventitious buds obtained in the step (3); (5) carrying out rooting induction culture on rootless test tube seedlings obtained in the step (4); and (6) carrying out domestication on sterile seedlings obtained in the step (5) and transplanting. When the tissue culture propagation method disclosed by the invention is used for cultivating seedlings of taxodium hybrids'zhongshansha118', the seedling growing time is short, the multiplication rate is 89-92 times, the rooting rate is 90%, the average rooting quantity is 4.0-4.1, and the domestication survival rate is up to 95%. With the method, seedlings of taxodium hybrids'zhongshansha118' can be effectively propagated and the market requirement on seedlings of taxodium hybrids'zhongshansha118' can be met to the great extent.

The invention, which belongs to the technical field of biology, relates to a detection primer, a kit and a detection method for endophytic fungi diversity of taxodium zhongshanshan. The detection primer comprises a first primer pair and a second primer pair. The sequences of the first primer pair are shown as SEQ ID NO.1 and SEQ ID NO.2; and the sequences of the second primer pair are shown as SEQID NO.3 and SEQ ID NO.4. The detection primer provided by the invention is obtained by analyzing and designing the diversity of leaf fungi of the taxodium zhongshanshan and has high sensitivity and detection concentration. The detection method provided by the invention is a detection scheme with simple steps and low cost; and a theoretical reference can be provided for detecting the diversity ofendophytic fungi in different varieties of leaves in different regions of the taxodium zhongshanshan.