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In-vitro propagation method of taxodium hybrids 'zhongshansha136'

A technology of in vitro propagation and varieties, applied in the direction of botany equipment and methods, fertilizer mixture, soilless cultivation, etc., to achieve the effect of improving germination rate

Inactive Publication Date: 2014-11-05
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Invention patent CN 102283126 B discloses Zhongshan fir 302 (the female parent of Zhongshan fir 136) tissue culture and multiplication method, but because Zhongshan fir 136 belongs to the F 2 The genotypes and metabolites of the plants of the first-generation hybrids and Zhongshanshanshan 302 are different, so they cannot be used for Zhongshanshan 136 by simply copying them.

Method used

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  • In-vitro propagation method of taxodium hybrids 'zhongshansha136'
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  • In-vitro propagation method of taxodium hybrids 'zhongshansha136'

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Experimental program
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Effect test

Embodiment 1

[0038] (1) On April 03, 2012, the branches of Zhongshan fir 136 with axillary buds that had just germinated in spring were selected as explants, and the branches were washed with tap water to remove the dirt on the surface of the branches, and then the redundant leaves were removed and disinfected: ①Use Wash with neutral detergent, rinse with running water for 2 hours, and absorb the surface moisture with filter paper; ② Soak in 70% alcohol on the ultra-clean workbench for 20-30 seconds, rinse with sterile water 3 times, shake for 3-5 minutes each time; ③ Immediately Transfer to 1‰ mercury chloride solution for disinfection for 7-10 minutes, and finally wash with sterile water for 3 to 5 times, shaking each time for 3 to 5 minutes.

[0039] (2) Preparation of improved 1 / 2MS medium: use Ca(NO 3 ) 2 .4H 2 O260mg / L replaces CaCl in the original 1 / 2MS medium 2 .2H 2 O, with K 2 SO 4 560mg / L instead of KNO in the original 1 / 2MS medium 3 , adding activated carbon at the same ...

Embodiment 2

[0049] (1) On March 27, 2013, the branches of Zhongshan fir 136 with axillary buds that had just germinated in spring were selected as explants, and the branches were washed with tap water to remove the dirt on the surface of the branches, and then the redundant leaves were removed and disinfected: ① with Wash with neutral detergent, rinse with running water for 2 hours, and absorb the surface moisture with filter paper; ② Soak in 70% alcohol on the ultra-clean workbench for 20-30 seconds, rinse with sterile water 3 times, shake for 3-5 minutes each time; ③ Immediately Transfer to 1‰ mercury chloride solution for disinfection for 7-10 minutes, and finally wash with sterile water for 3 to 5 times, shaking each time for 3 to 5 minutes.

[0050] (2) Preparation of improved 1 / 2MS medium: use Ca(NO 3 ) 2 .4H 2 O260mg / L replaces CaCl in the original 1 / 2MS medium 2 .2H 2 O, with K 2 SO 4 560mg / L instead of KNO in the original 1 / 2MS medium 3 , adding activated carbon at the sam...

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Abstract

The invention discloses an in-vitro propagation method of taxodium hybrids 'zhongshansha136'. The in-vitro propagation method includes the following steps that (1) explants are selected and disinfected; (2) a 1 / 2MS culture medium is improved; (3) the processed explants are cut, and stem primary induction culture and high-voltage electrostatic field (HVEF) processing are conducted; (4) secondary multiplication culture is conducted on adventitious buds obtained in the step (3); (5) root induction culture is conducted on rootless test-tube plantlets obtained in the step (4); (6) domestication seedling and transplanting are conducted on aseptic seedlings obtained in the step (5). The tissue culture propagation method is used for conducting seedling on the taxodium hybrids 'zhongshansha136', the seedling time is short, the reproduction rate is increased by 88-93 times, the rooting rate is 89%, the average root number is 4.1, the seedling survival rate reaches 95%, taxodium hybrids 'zhongshansha136' seedlings can be obtained efficiently, and the requirement for the taxodium hybrids 'zhongshansha136' seedlings of the market is met to a large extent.

Description

technical field [0001] The invention relates to an in vitro propagation method of Zhongshan fir variety 136 (Taxodium hybrids 'zhongshansha136'), belonging to the technical field of woody plant seedling propagation. Background technique [0002] Taxodium hybrids 'zhongshansha' belongs to the genus Taxodium in the family Taxodiaceae. Zhongshan fir is a semi-evergreen tall tree with a tall and majestic tree body and a beautiful tree shape. It is an excellent interspecies of three tree species: T.distichum, T.ascendens and T.mucronatum. The hybrid is bred by the Institute of Botany, Chinese Academy of Sciences, Jiangsu Province for many years. Zhongshan fir has the advantages of fast growth, water and humidity resistance, salt and alkali resistance, less pests and diseases, and strong wind resistance, so it is an excellent greening tree species in saline-alkali tidal flats. [0003] Because Zhongshan fir has straight texture, fine structure, soft material, corrosion and moth ...

Claims

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Application Information

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IPC IPC(8): A01G31/00C05G3/00
Inventor 陆小清李乃伟李云龙王传永陈红蔡小龙
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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