A kind of tissue culture propagation method of Zhongshan fir variety 118

A technology of tissue culture and varieties, applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effect of improving germination rate

CN104115752BInactive Publication Date: 2016-01-20INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2016-01-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a tissue culture propagation method of taxodium hybrids'zhongshansha118'. The method comprises the following steps: (1) selecting and disinfecting an explant; (2) improving a 1 / 2 MS culture medium; (3) cutting the treated explant, carrying out stem primary induction culture and treating in a high-voltage electrostatic field (HVEF); (4) carrying out subculture multiplication culture on adventitious buds obtained in the step (3); (5) carrying out rooting induction culture on rootless test tube seedlings obtained in the step (4); and (6) carrying out domestication on sterile seedlings obtained in the step (5) and transplanting. When the tissue culture propagation method disclosed by the invention is used for cultivating seedlings of taxodium hybrids'zhongshansha118', the seedling growing time is short, the multiplication rate is 89-92 times, the rooting rate is 90%, the average rooting quantity is 4.0-4.1, and the domestication survival rate is up to 95%. With the method, seedlings of taxodium hybrids'zhongshansha118' can be effectively propagated and the market requirement on seedlings of taxodium hybrids'zhongshansha118' can be met to the great extent.
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Description

technical field

[0001] The invention relates to a tissue culture propagation method of Zhongshan fir variety 118 (Taxodium hybrids 'zhongshansha118'), belonging to the technical field of woody plant seedling propagation. Background technique

[0002] Taxodium hybrids 'zhongshansha' belongs to the genus Taxodium in the family Taxodiaceae. Zhongshan fir is a semi-evergreen tall tree with a tall and majestic tree body and a beautiful tree shape. It is an excellent interspecies of three tree species: T.distichum, T.ascendens and T.mucronatum. The hybrid is bred by the Institute of Botany, Chinese Academy of Sciences, Jiangsu Province for many years. Zhongshan fir has the advantages of fast growth, water and humidity resistance, salt and alkali resistance, less pests and diseases, and strong wind resistance, so it is an excellent greening tree species in saline-alkali tidal flats.

[0003] Because Zhongshan fir has straight texture, fine structure, soft material, corrosion and ...

Examples

Embodiment 1

[0038] (1) On April 30, 2012, select spring Zhongshan fir 118 with just germinated branches of axillary buds as explants, wash the branches with tap water, remove the dirt on the surface of the branches, then remove redundant leaves, and disinfect: ① use Wash with neutral detergent, rinse with running water for 2 hours, and absorb the surface moisture with filter paper; ② Soak in 70% alcohol on the ultra-clean workbench for 20-30 seconds, rinse with sterile water 3 times, shake for 3-5 minutes each time; ③ Immediately Transfer to 1‰ mercury chloride solution for disinfection for 7-10 minutes, and finally wash with sterile water for 3 to 5 times, shaking each time for 3 to 5 minutes.

[0039] (2) Preparation of improved 1 / 2MS medium: use Ca(NO 3 ) 2 .4H 2 O228mg / L replaces CaCl in the original 1 / 2MS medium 2 .2H 2 O, with K 2 SO 4 730mg / L instead of KNO in the original 1 / 2MS medium 3 , adding activated carbon at the same time, the concentration is: 1500mg / L, other compon...

Embodiment 2

[0049] (1) On April 26, 2013, the branches of Zhongshan fir 118 with axillary buds that had just germinated in spring were selected as explants, and the branches were washed with tap water to remove the dirt on the surface of the branches, and then the redundant leaves were removed and disinfected: ① with Wash with neutral detergent, rinse with running water for 2 hours, and absorb the surface moisture with filter paper; ② Soak in 70% alcohol on the ultra-clean workbench for 20-30 seconds, rinse with sterile water 3 times, shake for 3-5 minutes each time; ③ Immediately Transfer to 1‰ mercury chloride solution for disinfection for 7-10 minutes, and finally wash with sterile water for 3 to 5 times, shaking each time for 3 to 5 minutes.

[0050] (2) Preparation of improved 1 / 2MS medium: use Ca(NO 3 ) 2 .4H 2 O228mg / L replaces CaCl in the original 1 / 2MS medium 2 .2H 2 O, with K 2 SO 4 730mg / L instead of KNO in the original 1 / 2MS medium 3 , adding activated carbon at the sam...