Method for quickly breeding Louisiana cypress in-vitro

An in vitro and rapid technology, applied in the field of plant tissue culture, can solve the problem that the in vitro rapid propagation method of Chinese fir has not yet been reported, and achieve the effects of saving seedlings occupying land, high reproduction coefficient, and saving foreign exchange.

Inactive Publication Date: 2009-01-21
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] So far, the in vitro rapid propagatio...

Method used

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  • Method for quickly breeding Louisiana cypress in-vitro
  • Method for quickly breeding Louisiana cypress in-vitro
  • Method for quickly breeding Louisiana cypress in-vitro

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1. Test materials

[0044] 1. Mature bald cypress seeds come from Louisiana, USA.

[0045] 2. Preparation of culture medium:

[0046] (1), the composition or preparation method of "SH basic medium";

[0047] Table 1 SH medium (Schenk and Hildebrandt, 1972)

[0048] SH I large number of elements

Ingredients of Vegetarian Mother Solution

content

/ mg·L -1

SH II trace elements

Ingredients of Vegetarian Mother Solution

content

/ mg·L -1

SH III iron salt mother

liquid component

content

/ mg·L -1

SH IV Organic

Compound Mother Liquor Components

content

/ mg·L -1

KNO 3

2500

h 3 BO 3

5.0

FeSO 4 ·7H 2 o

15.0

Inositol

1000

NH 4 h 2 PO4

300

ZnSO 4 ·7H 2 o

1.0

Na 2 EDTA·H 2 o

20.0

Thiamine hydrochloride

5.0

CaCl 2 2H 2 o

200

MnSO 4 ·H 2 o

10.0

Niacin...

Embodiment 2

[0070] Except the 6-BA used in the adventitious bud proliferation medium to 20 mg / liter, the IBA 5 mg / liter, and the addition of NAA to 5 mg / liter, the rest are the same as in Example 1.

Embodiment 3

[0072] Except that the agar used in the adventitious bud proliferation medium is 6.5 grams per liter, all the other are the same as in Example 1.

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Abstract

The invention discloses an in-vitro quick propagating method of Taxodium.distichum, which pertains to the field of plant tissue culture. The method of the invention comprises the following steps: (1) taking mature embryos of sterilized Taxodium.distichum seeds as explant; (2) induction culture of adventitious buds; (3) elongation culture of adventitious buds; (4) propagation culture of adventitious buds; (5) culture of sound plantlets; (6) induction culture of adventitious roots; (7) formation of regenerative plants; (8) plantlets hardening and transplanting; wherein the basic culture medium applied to each culture stage is SH culture medium; the growth regulator added at each culture stage has the most suitable use amount and proportion which are selected by orthogonal design experiments. The test-tube plantlets of the Taxodium.distichum bred by the method of the invention have strong growth capacity, high propagation coefficient, up to 80% of the inducing rooting rate, approximate 90% of the transplant survival rate, and can be used for large-scale and industrialized production.

Description

technical field [0001] The invention relates to a method for plant tissue culture, in particular to a method for in vitro rapid propagation of mature embryos of Taxodium distichum, belonging to the field of plant tissue culture. Background technique [0002] There are three tree species in the genus Taxodium Rich., namely: Taxodium distichum, Taxodium distichum, and Mexican taxodium; among them, Taxodium distichum is an ancient "relict plant", which was born in the late Jurassic to the early Cretaceous. Already flourishing, the Quaternary glaciers have all been extinct in Eurasia, and they have only been preserved and multiplied in parts of North America and Latin America. [0003] Judging from tree morphology and biological characteristics, Bald Cedar is a tall tree with a majestic and beautiful crown shape, luxuriant branches and leaves, and a late leaf-falling period. It has a beautiful perennial landscape and is very suitable for landscape greening. At the same time, th...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00C12N5/04
Inventor 曹福亮杨小虎汪贵斌朱灿灿
Owner NANJING FORESTRY UNIV
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