A kind of in vitro propagation method of Zhongshan fir variety 136

A technology of in vitro reproduction and varieties, applied in the direction of botany equipment and methods, fertilizer mixture, soilless cultivation, etc., to achieve the effect of improving germination rate
CN104126492BInactive Publication Date: 2016-04-13INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
Publication Date
2016-04-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an in-vitro propagation method of taxodium hybrids 'zhongshansha136'. The in-vitro propagation method includes the following steps that (1) explants are selected and disinfected; (2) a 1 / 2MS culture medium is improved; (3) the processed explants are cut, and stem primary induction culture and high-voltage electrostatic field (HVEF) processing are conducted; (4) secondary multiplication culture is conducted on adventitious buds obtained in the step (3); (5) root induction culture is conducted on rootless test-tube plantlets obtained in the step (4); (6) domestication seedling and transplanting are conducted on aseptic seedlings obtained in the step (5). The tissue culture propagation method is used for conducting seedling on the taxodium hybrids 'zhongshansha136', the seedling time is short, the reproduction rate is increased by 88-93 times, the rooting rate is 89%, the average root number is 4.1, the seedling survival rate reaches 95%, taxodium hybrids 'zhongshansha136' seedlings can be obtained efficiently, and the requirement for the taxodium hybrids 'zhongshansha136' seedlings of the market is met to a large extent.
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Description

technical field

[0001] The invention relates to an in vitro propagation method of Zhongshan fir variety 136 (Taxodium hybrids 'zhongshansha136'), belonging to the technical field of woody plant seedling propagation. Background technique

[0002] Taxodium hybrids 'zhongshansha' belongs to the genus Taxodium in the family Taxodiaceae. Zhongshan fir is a semi-evergreen tall tree with a tall and majestic tree body and a beautiful tree shape. It is an excellent interspecies of three tree species: T.distichum, T.ascendens and T.mucronatum. The hybrid is bred by the Institute of Botany, Chinese Academy of Sciences, Jiangsu Province for many years. Zhongshan fir has the advantages of fast growth, water and humidity resistance, salt and alkali resistance, less pests and diseases, and strong wind resistance, so it is an excellent greening tree species in saline-alkali tidal flats.

[0003] Because Zhongshan fir has straight texture, fine structure, soft material, corrosion and moth ...

Claims

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