Method for culturing and propagating tissues of Taxodium Zhongshanha 302

A technology of tissue culture and culture medium, applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve the problems of cumbersome cultivation process and complicated material collection methods
CN102283126BInactive Publication Date: 2012-11-07INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
1 Cites 4 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
Publication Date
2012-11-07
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a method for culturing and propagating tissues of Taxodium Zhongshanha 302. The method comprises the following steps: (1) selecting an explant and disinfecting the explant; (2) improving an original WPM (wood plant medium) culture medium; (3) after cutting the processed explant, performing primary induction and culture for stems; (4) performing secondary multiplication andculture for new tips obtained in the step (3); (5) performing rooting induction culture for the rootless test-tube plantlet obtained in the step (4); and (6) performing domesticating hardening off culture for the aseptic seedlings obtained in the step (5). The propagating method is adopted to culture seedlings of the Taxodium Zhongshanha 302, the propagating rate is 102-110 times and the seedlingculturing time is short.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention specifically relates to a tissue culture propagation method of Zhongshan fir variety 302 (Taxodium hybrids 'Zhongshansha302'), which belongs to the technical field of woody plant seedling propagation. Background technique

[0002] Zhongshan fir (Taxodium hybrids'Zhongshansha'), a general term for the excellent interspecific hybrid clones of Taxodiaceae (Taxodiaceae), composed of T.distichum, T.ascendens, Mexican cypress Cedar (T.mucronatum) three species of cross-breeding. Semi-evergreen tall tree with prominent trunk, conical or umbrella-shaped crown, compact and plump. The leaves are dense, pinnately compound, strip-shaped, 0.6-1.0 cm long, and spirally scattered on the branchlets, not in two rows. Dioecious, same plant. Female cones grow on the top of new branches, singly or in clusters of 2-3, spherical when mature, 3.5-5.0mm long. The male cones grow on the twigs and are elliptical when mature, 3.0-5.0mm long. The flowering peri...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More