A method for rapidly propagating Polygonatum test-tube plantlets

A test-tube seedling, Polygonatum chinensis technology, applied in the field of plant cultivation, can solve the problems of multiflora Polygonatum chinensis variety degradation, uneconomical, large amount of seed rhizomes and the like, and achieves the effects of improving the transplanting survival rate and the high pollution rate.

Active Publication Date: 2022-03-11
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Polygonatum rhizome is shaped like a potato, and people in mountainous areas often eat it as a vegetable. In addition, Polygonatum has good ornamental value. Therefore, Polygonatum combines medicinal, edible, and ornamental functions, and has a very high market prospect. The medicinal value and health value of Polygonatum polyflora are recognized by people, the demand for raw materials of Polygonatum polyflora is increasing, and the price is also rising, which leads to a large amount of predatory excavation of wild Polygonatum polyflora by farmers in mountainous areas , so that the natural resources of Polygonatum multiflora are rapidly exhausted, which brings adverse effects on the development, utilization and sustainable development of Polygonatum multiflora
[0003] At the same time, since Polygonatum multiflora mostly adopts split root propagation in production, the propagation coefficient is low, and the amount of seed rhizome is large, which is not economical and limits the large-scale planting of Polygonatum multiflora, and long-term split root propagation is easy to cause Degradation of Polygonatum species, especially changes in their active active ingredients
The problem of seedling production has become a major bottleneck in the artificial large-scale planting of Polygonatum polyflora

Method used

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  • A method for rapidly propagating Polygonatum test-tube plantlets
  • A method for rapidly propagating Polygonatum test-tube plantlets
  • A method for rapidly propagating Polygonatum test-tube plantlets

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: A method of rapid breeding of a yellow crison, which includes the following steps:

[0027] (1) Collect flowers 15 to 20 days of flowers, washed with detergent for 10min, then rinse with water 10min, bring into the inoculation room, over the workbench, remove the petals, only the sub-room part, 75% alcohol soak 45 ~ 60S, soak 5 min with disinfectant, rinse 3 times in sterile water.

[0028] (2) Aseptic sub-room obtained by step (1) is on the ultra-net workbench, and the chopper is cut out with an anatomic knife, and the invisible medium is induced, and dark culture is administered 15 days, and the light illuminance is 1000-1500. Lex, lighting time 12H / D; 30D after the seed embryo germinated and induced into a callus, the solid medium group is: 2,4-dichlorophenoxyacetic acid (2, 4-D) 0.5 ~ 1.0 Mg / L + sucrose 25 ~ 30 g / l + 1 / 2 ms medium + agar 5 ~ 7 g / l + pH 5.4 ~ 5.8.

[0029] (3) Inoculation of the callus (2) by the callus of Huangjing seed embryos induce...

Embodiment 2

[0034] As in Example 1, step (2), step (3), step (4), induction, callus proliferation, callus, callus, and cultivate four stages Screening is performed in a combination of the medium listed in Table 1 to preferably combine the formulation of the optimal medium. Table 1 shows the following:

[0035] In response to the production and different cultural stages, the cultural form, the main observation of the cryptus-induced, texture, color (light green (light green (light green is good), etc., the main observation of the recipe is reasonable; the callus proliferation phase is reasonable; It mainly observed that the callus increased the fast, texture, color (light green is good) and other indicators to judge whether the formulation is reasonable; the callus division is mainly observed by the ability to distinguish between callus differentiation, uncertain buds Degree, the degree of glassization (the lower the better), color and other indicators to judge the good and bad of the formulat...

Embodiment 3

[0046] Example 3: Subsequently, in order to obtain a yellow-crowned seedlings in Example 1, the callus can be omitted in step 3, i.e., the callus obtained in step 2 can be directly divided into seedlings.

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Abstract

The invention discloses a method for rapid propagation of Polygonatum test-tube seedlings. The specific steps are as follows: the flowers of Polygonatum 15-20 days after pollination are used as explants, and the immature embryos are taken out after laboratory disinfection and placed on a callus induction medium. After the induced callus is further proliferated, it is transferred to a differentiation medium to differentiate adventitious buds, and finally the adventitious buds are cultured for strong seedlings to take root to form Polygonatum tissue cultured plantlets. The invention can be directly used for factory-scale seedling production, and has the advantages of less pollution, fast multiplication speed, low mutation rate, neat and robust seedlings, low production cost, convenient transportation, high transplanting survival rate and the like.

Description

Technical field [0001] The present invention belongs to plant cultivation techniques, and specifically, a method of rapid reproduction of Huangjing testizer. Background technique [0002] Polygonatum Sibirum, also known as the chicken head, yellow chicken, yellow chicken, pen tube, for the lily of the lily, the root stem containing a variety of steroidal saponins and Huangjing low glycans, with nourishing Qi,,,, spleen, etc., commonly used in spleen and stomach weakness, physical fatigue, no food, lung deficiency, insufficient blood, insufficient blood, and thirst, simultaneous taste, sweet, edible tasty. Its meat root stem thickness, contains a large amount of starch, sugar, fat, protein, carotene, vitamin and a variety of other nutrients, raw food, stewing can be full of hunger, and fitness, can be plenty, muscle full The bone marrow is strong and is very beneficial to the body. The shape of the yellow stem roots is like a mountain. The people in the mountains often treat it as...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001A01H4/005
Inventor 史俊贾君沈正红席刚俊葛晓宽徐晓燕
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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