Method for improving transplanting survival rate of polygonatum cyrtonema tissue culture seedlings

Abstract

The invention belongs to the technical field of plant biology, and discloses a method for improving the transplanting survival rate of polygonatum cyrtonema tissue culture seedlings, which comprises the following steps: taking elongation buds obtained by polygonatum cyrtonema tissue culture as explants, separating the explants from adventitious bud clusters, treating bud bases with a rooting promoting solution, and transplanting the explants to obtain the polygonatum cyrtonema tissue culture seedlings. Inoculating into a culture medium for synchronous induction culture of adventitious roots and micro tubers; transferring the tissue culture bottle filled with the polygonatum cyrtonema regeneration plant with the induced micro tubers and adventitious roots to a plastic greenhouse under outdoor natural light for domestication; and transplanting the treated polygonatum kingianum tissue culture seedlings into a matrix, and carrying out seedling culture under a forest to obtain polygonatum cyrtonema regenerated plants. The method has the beneficial effects that the method capable of effectively improving the transplanting survival rate of the polygonatum cyrtonema tissue culture seedlings is provided, and certain technical support is provided for industrial production and transplanting of the polygonatum cyrtonema tissue culture seedlings.

Description

Technical Field

[0001] The invention relates to the field of plant biotechnology, and in particular to a method for improving the transplant survival rate of polygonatum cyrtonema tissue culture seedlings. Background Art

[0002] Polygonatum cyrtonema Hua, also known as "Xianren Yuliang", is a perennial herbaceous plant of the genus Polygonatum in the Liliaceae family. Its rhizome is used as medicine, and it has the effects of replenishing qi and nourishing yin, strengthening the spleen, moistening the lungs, and benefiting the kidneys. As one of the "Top Ten Anhui Medicines", Polygonatum cyrtonema Hua has been cultivated in large quantities in Anhui Province. It is a traditional bulk medicinal material that combines medicinal, edible, ornamental and health-care values. In recent years, with the development of traditional Chinese medicine and the big health industry, the medicinal and health-care values ​​of Polygonatum cyrtonema Hua have been increasingly recognized by people, and its market prospects are very broad.

[0003] The lack of excellent varieties and effective variety breeding technology is a bottleneck problem that restricts the development of the Polygonatum cyrtonema industry. The traditional sowing and tuber propagation methods are difficult to meet the growing demand of the Polygonatum cyrtonema seedling market due to the problems of low breeding efficiency, long breeding cycle, and breeding restricted by materials and seasons. Plant tissue culture technology has the advantages of high breeding efficiency, short breeding cycle, and breeding not restricted by season and time. It is of great significance in the preservation of Chinese medicinal plant resources and factory breeding. At present, there have been many reports on the research of plant tissue culture technology in the rapid propagation of Polygonatum cyrtonema seedlings, but in the process of tissue culture production and planting of Polygonatum cyrtonema seedlings, the low survival rate of field transplanting of tissue culture seedlings has become a key factor restricting the industrial production of tissue culture seedlings of Polygonatum cyrtonema.

[0004] Therefore, in view of the above problems, based on the tissue culture breeding of excellent strains of Polygonatum cyrtonema, a method for improving the transplant survival rate of Polygonatum cyrtonema tissue culture seedlings is urgently needed to meet the production needs of large-scale planting of Polygonatum cyrtonema tissue culture seedlings. Summary of the invention

[0005] The technical problem to be solved by the present invention is how to improve the transplant survival rate of Polygonatum cyrtonema tissue culture seedlings.

[0006] The present invention solves the above technical problems through the following technical means:

[0007] A method for improving the survival rate of transplanting Polygonatum cyrtonema tissue culture seedlings comprises the following steps:

[0008] (1) Synchronous induction culture of adventitious roots and microtubers: The elongated buds obtained by tissue culture of Polygonatum cyrtonema were used as explants, separated from the adventitious bud clusters, and the bud bases were treated with rooting promotion liquid and then inoculated into culture medium for synchronous induction culture of adventitious roots and microtubers;

[0009] (2) Treatment of Polygonatum cyrtonema tissue culture seedlings before transplanting: The tissue culture bottles containing the regenerated plants of Polygonatum cyrtonema with induced microtubers and adventitious roots were transferred to a plastic greenhouse outdoors under natural light for acclimatization;

[0010] (3) Transplantation and seedling management of Polygonatum cyrtonema tissue culture seedlings: The treated Polygonatum cyrtonema tissue culture seedlings were transplanted into the substrate and cultured under the forest to obtain regenerated plants of Polygonatum cyrtonema.

[0011] Preferably, in (1), the Polygonatum cyrtonema is Qiyuan Polygonatum cyrtonema.

[0012] Preferably, in (1), the rooting promoting solution is an aqueous solution added with a final concentration of 500-1000 mg / L NAA, 250-1000 mg / L IBA and 10-100 mg / L ABA.

[0013] Preferably, in (1), the treatment time of the rooting promoting solution is 60-180 seconds.

[0014] Preferably, in (1), the culture medium is 1 / 4-2MS culture medium supplemented with 10-60 g / L sucrose and 7.0 g / L agar.

[0015] Preferably, in (1), the synchronous induction culture of adventitious roots and microtubers is carried out in a greenhouse with a temperature of 25±2°C, a light intensity of 2000-2500 lx, a photoperiod of 14 / 10h (light / darkness), and a light quality of one or more of blue light, red light, and white light.

[0016] Preferably, in (1), the period of synchronous induction culture is 6 weeks.

[0017] Preferably, in (2), the greenhouse acclimatization time is 7-10 days.

[0018] Preferably, in (2), the method further comprises soaking the greenhouse-acclimated tissue culture seedlings in a 0.01%-0.05% potassium permanganate solution containing 10-60 μM melatonin for 5-10 minutes.

[0019] Preferably, in (3), the steps are specifically included: transplanting the treated Polygonatum cyrtonema tissue culture seedlings into a seedling bag filled with garden soil: perlite: nutrient soil volume ratio = 4-8: 1-2: 1-4 and cultivating them under a forest at an altitude of 300-600m for seedling cultivation, spraying 1 / 8-1 / 2MS macroelements on the leaves after transplanting, covering with a sunshade net, and removing the sunshade net after 4 weeks. During this period, watering once and spraying 1 / 8-1 / 2MS macroelements once every 2 weeks to obtain regenerated Polygonatum cyrtonema plants.

[0020] The advantages of the present invention are:

[0021] The method for improving the transplant survival rate of Polygonatum cyrtonema tissue culture seedlings provided by the present invention has the following outstanding advantages: the developed technology for synchronous induction of adventitious roots and microtubers of elongated buds of Polygonatum cyrtonema tissue culture and the cultivation and management technology realize the synchronous induction of adventitious roots and microtubers of Polygonatum cyrtonema tissue culture seedlings and direct under-forest cultivation, greatly improving the transplant survival rate of Polygonatum cyrtonema tissue culture seedlings while simplifying the transplant steps of tissue culture seedlings, the under-forest transplant survival rate is as high as 96.8%, and provides important technical support for the industrialized production and planting of Polygonatum cyrtonema tissue culture seedlings. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 The regenerated plants of Polygonatum cyrtonema obtained by culturing for 6 weeks using conventional tissue culture technology in Example 1 of the present invention only have adventitious roots but no microtubes;

[0023] Figure 2 The regenerated plant of Polygonatum cyrtonema obtained by culturing for 4 weeks using the synchronous induction technology of adventitious roots and microtubers in Example 1 of the present invention;

[0024] Figure 3 The regenerated plant of Polygonatum cyrtonema obtained by culturing for 6 weeks using the adventitious root and microtuber synchronous induction technology in Example 1 of the present invention;

[0025] Figure 4 The invention relates to cleaning and drying of the regenerated plants of Polygonatum cyrtonema through tissue culture in an embodiment of the present invention;

[0026] Figure 5 The regenerated plant of Polygonatum cyrtonema transplanted for one month in Example 1 of the present invention;

[0027] Figure 6 This is a complete regenerated plant of Polygonatum cyrtonema that has been transplanted for 2 months in Example 1 of the present invention. DETAILED DESCRIPTION

[0028] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] Unless otherwise specified, the test materials and reagents used in the following examples can be obtained from commercial sources.

[0030] If no specific techniques or conditions are specified in the examples, they can be carried out according to the techniques or conditions described in the literature in the art or according to the product instructions.

[0031] Example 1

[0032] A method for improving the survival rate of transplanting Polygonatum cyrtonema tissue culture seedlings, the specific operation is as follows:

[0033] (1) After separating the uniformly growing and robust elongated buds of Polygonatum cyrtonema with a height of about 2.5-3.0 cm (the method for obtaining the elongated buds is derived from the Chinese patent application document with application number 202211199919.9, i.e., the adventitious buds of Polygonatum cyrtonema obtained after step (4) of Example 1 of the application document) from the adventitious bud cluster, one part as the control group is directly inoculated into the adventitious root induction medium and culture conditions involved in Example 2 (6) of the Chinese patent application document with application number 202211199916.5 for adventitious root induction culture; the other part as the experimental group is immersed at the base in a medium supplemented with a final concentration of 1000 mg / L NAA, 750 mg / L IBA and 25 mg / L The treated elongated shoots were inoculated in 1 / 2MS medium supplemented with 40 g / L sucrose and 7.0 g / L agar in a greenhouse at 25 ± 2 °C, 2000 lx light intensity, 14 / 10 h (light / darkness), and blue light quality for synchronous induction of adventitious roots and microtubers. After 6 weeks of light culture, adventitious roots were induced at the base of the elongated shoots of Polygonatum cyrtonema in the control group, but no microtubers were formed. On average, each explant produced 6.8 adventitious roots, and the average root length of the adventitious roots was 2.1 cm ( Figure 1 ). After the tissue culture bottles containing the regenerated plants of Qiyuan Polygonatum in the control group were moved to a plastic greenhouse outdoors under natural light for 7-10 days of acclimatization, the Polygonatum cyrtonema tissue culture seedlings were taken out and the roots were rinsed under running water, and soaked in a 0.05% potassium permanganate solution with 40μM melatonin for 7.5 minutes. The treated Polygonatum cyrtonema tissue culture seedlings were transplanted into seedling bags filled with garden soil: perlite: nutrient soil volume ratio = 6:1:2 and cultured under the forest at an altitude of 300-600m for seedling cultivation. After transplanting, 1 / 4MS macroelements were sprayed on the leaves. Cover with a shade net and remove the shade net after 4 weeks. During this period, water was poured once every 2 weeks and 1 / 4MS macroelements were sprayed once. After 2 months of transplanting, the average transplant survival rate was only 75.6%; while for the Polygonatum cyrtonema tissue culture elongation buds in the experimental group, adventitious roots were formed at the base of the stem after 4 weeks of light culture, accompanied by microtuber formation ( Figure 2 ), after 2 weeks of continuous light culture, the synchronous induction rate of adventitious roots and microtubers reached 96.5%, with an average of 12.3 adventitious roots per explant and an average adventitious root length of 5.4 cm ( Figure 3 ).

[0034] (2) The tissue culture bottles containing the regenerated plants of Qiyuan Polygonatum with induced microtubers and robust adventitious roots were moved to a plastic greenhouse outdoors under natural light for acclimatization for 7-10 days; then the Polygonatum cyrtonema tissue culture seedlings were taken out and the roots were rinsed under running water, and soaked in a 0.05% potassium permanganate solution with 40 μM melatonin added for 7.5 minutes, and the surface moisture was dried and set aside. Figure 4 ).

[0035] (3) Transplant the treated Polygonatum cyrtonema tissue culture seedlings into a seedling bag filled with garden soil: perlite: nutrient soil volume ratio = 6:1:2 and culture them under a forest at an altitude of 300-600m for seedling cultivation. Spray 1 / 4 MS macronutrients on the leaves after transplanting. Cover with a sunshade net and remove the sunshade net after 4 weeks. During this period, water once every 2 weeks and spray 1 / 4 MS macronutrients once. One month after transplanting, new leaves grow after the original tissue culture leaves of the Polygonatum cyrtonema regenerated plants fall off ( Figure 5 ); Two months after transplanting, the plants were strong and green, with an average plant height of 7.3 cm and an average transplant survival rate of 96.8% ( Figure 6 ).

[0036] Example 2

[0037] This example tests the effect of exogenous plant growth regulators on the synchronous induction of adventitious roots and microtubers of Polygonatum cyrtonema tissue culture elongated shoots. The specific steps are as follows:

[0038] (1) Uniform and robust elongated shoots of Polygonatum cyrtonema with a height of about 2.5-3.0 cm (obtained by the method described in Example 1) were separated from the adventitious bud clusters, and the bases were immersed in an aqueous solution supplemented with different concentrations of NAA (0, 250, 500, 750 and 1000 mg / L), IBA (0, 250, 500, 750 and 1000 mg / L) and ABA (0, 10, 25, 50, 75 and 100 mg / L) for 60 s. The treated elongated shoots were then inoculated into MS medium supplemented with 30 g / L sucrose and 7.0 g / L agar at a temperature of 25±2°C, a light intensity of 2000 lx, and a photoperiod of 14 / 10 h (light / dark) under LED blue light conditions for synchronous induction culture of adventitious roots and microtubers. During the culture period, the induction of adventitious roots and microtubers was observed from time to time. After 6 weeks of light culture, the induction rate of adventitious roots and microtubers was counted, and the average number of adventitious roots produced by each explant and the length of adventitious roots were calculated. The results are shown in Table 1. The results show that in the synchronous induction process of adventitious roots and microtubers of Polygonatum cyrtonema, exogenous plant growth regulators NAA, IBA and ABA play a synergistic role within a certain concentration range, and high concentrations of ABA will inhibit the formation of adventitious roots and microtubers. Among them, when the base of the elongated buds of Polygonatum cyrtonema was placed in an aqueous solution with a final concentration of 1000 mg / L NAA, 750 mg / L IBA and 25 mg / L ABA for 60 seconds, the induction effect of adventitious roots was the best, the synchronous induction efficiency of adventitious roots and microtubers was 77.9%, and an average of 5.2 adventitious roots were produced per explant, and the average length of the adventitious roots was 4.4 cm.

[0039] Table 1 Effects of different exogenous NAA, IBA and ABA concentrations on the synchronous induction of adventitious roots and microtubers of elongated shoots in Polygonatum cyrtonema

[0040]

[0041] Note: Data are mean values, each treatment contained 120 explants, and each treatment was repeated three times.

[0042] Example 3

[0043] This example tests the effects of exogenous NAA, IBA and ABA treatment time and sucrose concentration on the synchronous induction of adventitious roots and microtubers of Polygonatum cyrtonema tissue culture. The specific steps are as follows:

[0044] (1) Uniform and robust elongated shoots of Polygonatum cyrtonema with a height of about 2.5-3.0 cm (obtained by the method described in Example 1) were separated from the adventitious bud clusters, and the bases were immersed in an aqueous solution supplemented with 1000 mg / L NAA, 750 mg / L IBA and 25 mg / LABA for different treatment times (30, 60, 90, 120 and 180 s). The treated elongated shoots were then inoculated into MS medium supplemented with different concentrations of sucrose (20, 30, 40 and 60 g / L) and 7.0 g / L agar at a temperature of 25±2°C, a light intensity of 2000 lx, and a photoperiod of 14 / 10 h (light / dark) under LED blue light conditions for synchronous induction culture of adventitious roots and microtubers. During the culture period, the induction of adventitious roots and microtubers was observed from time to time. After 6 weeks of light culture, the induction rate of adventitious roots and microtubers was counted, and the number of adventitious roots produced by each explant and the length of the adventitious roots were averaged. The research results are shown in Table 2. The research results show that in the synchronous induction process of adventitious roots and microtubers of Polygonatum cyrtonema, the treatment time of exogenous plant growth regulators and the concentration of sucrose have an important regulatory effect on the synchronous induction of adventitious roots and microtubers. Within a certain range, with the extension of the treatment time of plant growth regulators and the increase of sucrose concentration, the synchronous induction rate of adventitious roots and microtubers of Polygonatum cyrtonema gradually increased. When the base of the elongated shoots of Polygonatum cyrtonema was placed in an aqueous solution with a final concentration of 1000 mg / L NAA, 750 mg / L IBA and 25 mg / L ABA for 90 seconds and then inoculated in MS medium with 40 g / L sucrose and 7.0 g / L agar, the induction effect of adventitious roots was the best, and the synchronous induction efficiency of adventitious roots and microtubers was 89.4%, with an average of 8.3 adventitious roots per explant and an average length of 5.1 cm. Then, with the extension of treatment time and the increase of sucrose concentration, the synchronous induction efficiency of adventitious roots and microtubers showed a downward trend.

[0045] Table 2 Effects of different treatment times of exogenous plant growth regulators and sucrose concentration in the culture medium on the synchronous induction of adventitious roots and microtubers of elongated shoots in Polygonatum cyrtonema

[0046]

[0047] Note: Data are mean values, each treatment contained 120 explants, and each treatment was repeated three times.

[0048] Example 4

[0049] This example tests the effects of macroelements in the culture medium and LED light quality on the synchronous induction of adventitious roots and microtubers of Polygonatum cyrtonema tissue culture. The specific steps are as follows:

[0050] (1) Uniform and robust elongated shoots of Polygonatum cyrtonema with a height of about 2.5-3.0 cm (obtained by the method described in Example 1) were separated from the adventitious bud clusters, and the bases were immersed in an aqueous solution supplemented with 1000 mg / L NAA, 750 mg / L IBA and 25 mg / LABA for 90 s. The treated elongated shoots were then inoculated into MS medium supplemented with 40 g / L sucrose, 7.0 g / L agar and different multiples of macroelements (1 / 4, 1 / 2, 1) at a temperature of 25±2°C, a light intensity of 2000 lx, a photoperiod of 14 / 10 h (light / darkness) and different LED light qualities (white light, red light, blue light and a red-blue light quality ratio of 3:1) for synchronous induction culture of adventitious roots and microtubers. During the culture period, the induction of adventitious roots and microtubers was observed from time to time. After 6 weeks of light culture, the induction rate of adventitious roots and microtubers was counted, and the average number of adventitious roots produced by each explant and the length of the adventitious roots were calculated. The research results are shown in Table 3. The research results show that during the synchronous induction of adventitious roots and microtubers of Polygonatum cyrtonema, the content of macroelements in the culture medium and the quality of LED light have an important regulatory effect on the synchronous induction of adventitious roots and microtubers of Polygonatum cyrtonema. Compared with white light, LED blue light can effectively promote the synchronous formation of adventitious roots and microtubers of Polygonatum cyrtonema. Further studies have found that within a certain range, as the macroelements in the culture medium decrease, the synchronous induction rate of adventitious roots and microtubers of Polygonatum cyrtonema gradually increases. When the base of the elongated buds of Polygonatum cyrtonema was placed in an aqueous solution supplemented with a final concentration of 1000 mg / L NAA, 750 mg / L IBA and 25 mg / L ABA and treated for 90 seconds, and then inoculated in a 1 / 2MS medium supplemented with 40 g / L sucrose and 7.0 g / L agar, the induction effect of adventitious roots was the best, and the synchronous induction efficiency of adventitious roots and microtubers was 96.5%. On average, 12.3 adventitious roots were produced per explant, and the average length of the adventitious roots was 5.4 cm.

[0051] Table 3 Effects of LED light quality and macronutrient content in culture medium on synchronous induction of adventitious roots and microtubers of elongated shoots in Polygonatum cyrtonema

[0052]

[0053] Note: Data are mean values, each treatment contained 120 explants, and each treatment was repeated three times.

[0054] Example 5

[0055] This example tests the effects of exogenous melatonin concentration and transplanting matrix ratio on the survival rate of transplanting Polygonatum cyrtonema tissue culture seedlings. The specific steps are as follows:

[0056] The tissue culture bottles containing the Qiyuan Polygonatum regeneration plants induced with microtubes and robust adventitious roots obtained in Example 1-4 were moved to a plastic greenhouse under natural light outdoors for 7 days; then the Polygonatum tissue culture seedlings were taken out and the roots were rinsed under running water, and soaked in a 0.05% potassium permanganate solution with different concentrations (0, 10, 20, 40 and 60uM) of melatonin for 7.5 minutes. After drying the surface moisture, they were transplanted into seedling bags filled with different ratios of nutrient matrix (garden soil: perlite: nutrient soil volume ratio = 4-8: 1-2: 1-4) under the forest at an altitude of 300-600m for seedling cultivation. After transplanting, 1 / 4MS macroelements were sprayed on the leaves. Cover with a sunshade net and remove the sunshade net after 4 weeks. During this period, water was poured once every 2 weeks and 1 / 4MS macroelements were sprayed once. The transplant survival rate was counted after 2 months of transplanting.

[0057] Table 4 Effects of exogenous melatonin concentration and transplanting substrate ratio on the survival rate of transplanted Polygonatum cyrtonema tissue culture seedlings

[0058]

[0059] Note: Data are mean values, each treatment contained 120 explants, and each treatment was repeated three times.

[0060] The research results are shown in Table 4. The research results show that in the process of transplanting Polygonatum cyrtonema tissue culture seedlings, the exogenous melatonin concentration and the ratio of nutrient matrix have an important influence on the transplant survival rate of Polygonatum cyrtonema tissue culture seedlings. The research results show that a certain concentration of melatonin immersion treatment can effectively improve the transplant survival rate of Polygonatum cyrtonema tissue culture seedlings. It is speculated that the reason may be that the appropriate concentration of melatonin can effectively promote the growth of Polygonatum cyrtonema tissue culture seedlings and improve their tolerance to environmental stress. The research results show that after the Polygonatum cyrtonema tissue culture seedlings were treated with a 0.05% potassium permanganate solution with a mass fraction of 40μM melatonin, they were transplanted into a nutrient matrix of V garden soil: V perlite: V nutrient soil = 6:1:2 and cultured for 2 months, the highest transplant survival rate was 96.8%.

[0061] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for improving the survival rate of transplanting Polygonatum cyrtonema tissue culture seedlings, characterized in that: The following steps are involved: (1) Synchronous induction culture of adventitious roots and microtubers: The elongated buds obtained by tissue culture of Polygonatum cyrtonema were used as explants, separated from the adventitious bud clusters, and the bud bases were treated with rooting promotion liquid and then inoculated into culture medium for synchronous induction culture of adventitious roots and microtubers; (2) Treatment of Polygonatum cyrtonema tissue culture seedlings before transplanting: The tissue culture bottles containing the regenerated plants of Polygonatum cyrtonema with induced microtubers and adventitious roots were transferred to a plastic greenhouse outdoors under natural light for acclimatization; (3) Transplantation and seedling management of Polygonatum cyrtonema tissue culture seedlings: The treated Polygonatum cyrtonema tissue culture seedlings were transplanted into the substrate and cultured under the forest to obtain regenerated plants of Polygonatum cyrtonema.

2. The method for improving the survival rate of transplanting Polygonatum cyrtonema tissue culture seedlings according to claim 1, characterized in that: In (1), the Polygonatum cyrtonema is Qiyuan Polygonatum.

3. The method for improving the survival rate of transplanting Polygonatum cyrtonema tissue culture seedlings according to claim 1, characterized in that: In (1), the rooting promoting solution is an aqueous solution added with a final concentration of 500-1000 mg / L NAA, 250-1000 mg / L IBA and 10-100 mg / L ABA.

4. The method for improving the survival rate of transplanting Polygonatum cyrtonema tissue culture seedlings according to claim 1, characterized in that: In (1), the treatment time of the rooting promoting solution is 60-180s.

5. The method for improving the survival rate of transplanting Polygonatum cyrtonema tissue culture seedlings according to claim 1, characterized in that: In (1), the culture medium is 1 / 4-2MS medium supplemented with 10-60 g / L sucrose and 7.0 g / L agar.

6. The method for improving the survival rate of transplanting Polygonatum cyrtonema tissue culture seedlings according to claim 1, characterized in that: In (1), synchronous induction culture of adventitious roots and microtubers is carried out in a greenhouse with a temperature of 25±2°C, a light intensity of 2000-2500 lx, a photoperiod of 14 / 10 h (light / darkness), and a light quality of one or more of blue light, red light, and white light.

7. The method for improving the survival rate of transplanting Polygonatum cyrtonema tissue culture seedlings according to claim 1, characterized in that: In (1), the synchronous induction culture period was 6 weeks.

8. The method for improving the survival rate of transplanting Polygonatum cyrtonema tissue culture seedlings according to claim 1, characterized in that: In (2), the greenhouse acclimatization time is 7-10 days.

9. The method for improving the survival rate of transplanting Polygonatum cyrtonema tissue culture seedlings according to claim 1, characterized in that: In (2), the method further includes soaking the greenhouse-acclimated tissue culture seedlings in a 0.01%-0.05% potassium permanganate solution containing 10-60 μM melatonin for 5-10 minutes.

10. The method for improving the survival rate of transplanting tissue culture seedlings of Polygonatum cyrtonema according to any one of claims 1 to 9, characterized in that: In (3), the steps are specifically included: transplanting the treated Polygonatum cyrtonema tissue culture seedlings into seedling bags filled with garden soil: perlite: nutrient soil volume ratio = 4-8: 1-2: 1-4 and cultivating them under the forest at an altitude of 300-600m for seedling cultivation, spraying 1 / 8-1 / 2MS macroelements on the leaves after transplanting, covering with a sunshade net, and removing the sunshade net after 4 weeks. During this period, watering once and spraying 1 / 8-1 / 2MS macroelements once every 2 weeks to obtain regenerated Polygonatum cyrtonema plants.

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