A method suitable for mass production of various orchid medicinal plants

Abstract

The invention discloses a method suitable for batch production of various orchid medicinal plants. The present invention designs a cultivation method according to production needs, and in the stage of obtaining high-efficiency basic seedlings, increases the concentration of cytokinin and auxin in the medium components, promotes bud germination, and inhibits root formation; adds 1.2 times of organic components and coconut milk to the medium Promote its growth and germination, shorten the germination time, increase the multiplication factor, and accelerate the reproduction of basic seedlings. At least 50 effective buds can be obtained per bottle, and the maximum is controlled within 100, so as to prevent too many seedlings from affecting the quality of basic seedlings. The inventive method not only improves the cultivation efficiency, but also saves the production cost.

Description

technical field [0001] The invention belongs to the technical field of tissue culture of orchid medicinal plants, and in particular relates to a method suitable for mass production of various orchid medicinal plants. Background technique [0002] Orchidaceae is the most endangered plant group in the world. Orchidaceae is the family with the most threatened species listed in the Red List of the International Union for Conservation of Nature (IUCN), and has become the "flagship" group in plant protection. Dendrobium officinale Kimura et Migo and Dendrobiumhuoshanense are Dendrobium officinale and Dendrobium huoshanense, which are perennial herbaceous plants. "Dao Zang" is known as "the first of the nine Chinese immortal grasses". Clematis 〔Anoectochilus roburghii (Wall.) Lindl.〕is a perennial herb of the Orchidaceae roburghii genus. Because of its high medicinal value, it is called the "King of Medicine". Bletilla striata (Bletilla striata (Thunb.) Reichb.f) is a perennial h...

AI Technical Summary

Problems solved by technology

[0004] In recent years, researchers have carried out a lot of industrial technology research on tissue culture and rapid propagation of Orchidaceae medicinal plants, especially in 2015, they provided a method for mass production of Dendrobium candidum tissue culture in one step into seedlings (authorization number ZL201510460584.5) , realized the simultaneous multiplication and differentiation of dendrobium protocorms, strong seedlings and rooting culture and other procedures simplified into a one-step method, but when using the inventive method for large-scale industrial production, it was found that many types of problems affecting the production of high-quality seedlings

The main problems are as follows: First, in the stage of basic seedling propagation, the number of subcultures of protocorms exceeds 5 times, the accumulation of hormones in the tissue gradually increases, and the growth of protocorms with pinhead-sized particles is very strong, gradually reducing the formation of differentiated seedlings, thus affecting the production process The quality and quantity of effective seedlings in the figure 1 , figure 2 , image 3 shown; the second is the mass production stage, which does not carry granular protocorms, but the components of the rooting medium for clustered shoots still contain relatively high concentrations of cytokinins and auxins. At the same time, a root system is formed at the base, and a large number of small buds will also be formed, resulting in uneven seedlings in the bottle, which are not suitable for transplanting in a whole bottle. They must be separated on an ultra-clean workbench, which not only reduces work efficiency, but also increases the chance of contamination. growth conditions such as Figure 4 , Figure 5 shown

If the suitable explants and tissue culture methods of different varieties are different, it will take a lot of time and energy to explore

Therefore, the above-mentioned reasons have seriously restricted the industrialization of high-quality seedlings of orchid medicinal plants.

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