28 results about "Dendrobium huoshanense" patented technology

The invention relates to a Tulasnella sp. QYM-1, a culture method of dendrobium huoshanense and application of the Tulasnella sp. QYM-1, and belongs to the technical field of microorganisms. The invention provides the mucilaginoidea fungus QYM-1, and the mucilaginoidea fungus QYM-1 is separated from protocorm formed by symbiotic germination, can promote germination of dendrobium huoshanense seeds to form seedlings, has relatively strong specificity on culture of the dendrobium huoshanense seeds, can replace tissue culture to become a main technology for seedling culture of dendrobium huoshanense, and has a broad application prospect in the field of seedling culture of dendrobium huoshanense. And the method has extremely high application value in production. In addition, the mucilaginoidea fungus QYM-1 is low in production cost and suitable for large-area popularization and application, and has huge research potential in the aspects of regression of rare and endangered orchid plants, wild-imitating cultivation of medicinal orchid plants and the like.

1. The name of the design product: beverage bottle (winding bottle). 2. The use of the design product: the design product is used for beverage bottles. 3. The design points of the design product: the shape of the product, the design is derived from the spiral shape of the Dendrobium huoshanense, the spiral-shaped ribs on the bottle body. 4. The picture or photo that best indicates the design points: design 1 perspective view. 5. Design 1 is designated as the basic design.

The invention relates to the technical field of dendrobium huoshanense processing equipment, in particular to a dendrobium huoshanense low-temperature wall-breaking grinding device which comprises a shell; the crushing and grinding unit is connected with the shell; collecting subrack boxes; the cooling unit is connected with the shell; wherein the smashing and grinding unit comprises a smashing and screening assembly, a material guiding assembly, a transmission control assembly and a grinding assembly, the smashing and screening assembly is fixedly connected with the shell wall of the top end of the shell, the grinding assembly is arranged between the smashing and screening assembly and the inserted collection insertion box, and the grinding assembly is slidably connected with the shell. A material guiding assembly is arranged between the grinding assembly and the smashing and screening assembly, the material guiding assembly is in sliding connection with the inner wall of the shell and connected with a transmission control assembly arranged on the shell, and the transmission control assembly is connected with the smashing and screening assembly. And the grinding efficiency and quality are greatly improved.

The invention discloses a mucilaginosa N15 and a method for promoting the germination of dendrobium huoshanense seeds. The mucilaginosa N15 is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on October 27, 2025, and the preservation number of the strain is CGMCC No: 42290. The invention further discloses a method for promoting the germination of dendrobium huoshanense seeds by using the mucilaginosa N15 and the method for promoting the germination of dendrobium huoshanense seeds by using the mucilaginosa N15. The strain can be prepared into a high-activity microbial agent after activation and amplification culture, and the high-activity microbial agent is mixed with dendrobium huoshanense seeds and then sown in a greenhouse, so that the seed germination efficiency can be remarkably improved. By applying the method, the germination rate of the dendrobium huoshanense seeds can be increased to 60% or above, the mycorrhiza infection rate reaches 80% or above, the comprehensive cost is reduced by 30%, the seedling transplanting survival rate reaches 90% or above, and the seedling raising period is shortened to 12-15 months. The method is easy and convenient to operate, low in cost, high in transplanting survival rate and suitable for large-scale seedling raising and resource protection of dendrobium huoshanense, and has important economic and ecological values.

The invention discloses application of glycosyl transferase coded by a dendrobium huoshanense DhUGT44 gene in biosynthesis of cosmosiin, and belongs to the technical field of biology. The nucleotide sequence of the DhUGT44 gene encodes a nucleotide sequence of an amino acid sequence as shown in SEQ ID NO. 2. The cosmosiin is obtained by a biosynthesis method for carrying out enzyme catalysis by utilizing the DhUGT44 protein in vitro, and a new method is provided for synthesizing the cosmosiin.

The invention discloses a dendrobium huoshanense compound traditional Chinese medicine composition for treating dysmenorrhea and a preparation method of the dendrobium huoshanense compound traditional Chinese medicine composition, and belongs to the field of traditional Chinese medicine compounds. The dendrobium huoshanense compound traditional Chinese medicine composition for treating dysmenorrhea comprises 6-12 parts of dendrobium huoshanense, 9-15 parts of glossy privet fruit, 12-18 parts of radix paeoniae alba, 9-15 parts of dogwood, 9-15 parts of radix rehmanniae recen, 6-12 parts of cortex lycii radicis, 9-15 parts of teasel root, 6-12 parts of eucommia ulmoides, 3-9 parts of honey-fried licorice root and 6-12 parts of achyranthes bidentata. The invention also provides a preparation method of the composition. Compared with the prior art, the prepared dendrobium huoshanense compound traditional Chinese medicine composition for treating dysmenorrhea has the advantage of being good in effect of treating dysmenorrhea caused by deficiency of liver and kidney, yin deficiency and internal heat.

The invention relates to the technical field of transgenosis, and discloses a method for transgenosis by using dendrobium huoshanense protocorm, which comprises the following steps: S1, induction culture of dendrobium huoshanense protocorm; s2, constructing a plant expression vector; s3, infecting and culturing the dendrobium huoshanense protocorm by agrobacterium tumefaciens; s4, screening and culturing the dendrobium huoshanense transgenic tissue and seedlings, the dendrobium huoshanense protocorm is used as a material, agrobacterium tumefaciens-mediated genetic transformation is realized on the basis of a dendrobium huoshanense protocorm tissue rapid propagation system, a stable and rapid genetic transformation system of dendrobium huoshanense is established, and creation of a new variety of dendrobium huoshanense is realized.

The invention belongs to the technical field of microorganisms, and discloses a Tulasnella sp. Strain and application of the Tulasnella sp. Strain. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) The strain has a remarkable promoting effect on seed germination, seedling growth, seedling rooting and accumulation of polysaccharide content in stems of dendrobium officinale and dendrobium huoshanense.

1. The name of this design product: Packaging box (Huoshan Dendrobium). 2. Purpose of this design product: used for packaging of Chinese medicinal materials. 3. The key design points of this product are the combination of shape, pattern and color. 4. The picture or photo that best illustrates the design points: three-dimensional picture. 5. The design for which protection is sought includes color.

1.The name of the design product: packaging box (special for Dendrobium huoshanense). 2.The use of the design product: the design product is used for the outer packaging of Dendrobium huoshanense. 3.The design points of the design product: the combination of shape, pattern and color. 4.The picture or photo that best shows the design points: perspective view. 5.The design claimed contains color.

The invention discloses a dendrobium huoshanense UGT70 gene coded glycosyl transferase and application thereof in synthesis of antioxidant flavonoid bioglycosides, and belongs to the technical field of biology. Nucleotide sequence coding of the UGT70 gene is an amino acid sequence as shown in SEQ ID NO. 2. The flavonoid diglucoside compound is obtained by a biosynthesis method for carrying out enzyme catalysis by utilizing UGT70 protein in vitro, and a new method is provided for synthesizing the flavonoid diglucoside compound.

The invention relates to the technical field of tissue culture, in particular to a tissue culture control method for increasing the content of polysaccharide in protocorm of dendrobium huoshanense, the method is carried out in a bioreactor provided with an online monitoring system, and the online monitoring system is at least used for monitoring a culture characteristic parameter set of a culture solution in real time; the method comprises the following steps: culturing dendrobium huoshanense protocorm under a first culture condition suitable for cell proliferation; judging whether cell growth enters a proliferation saturation state or not based on a culture characteristic parameter set monitored by an online monitoring system in real time; if yes, triggering a polysaccharide induction stage, and taking the culture characteristic parameter set for triggering the polysaccharide induction stage as a reference culture characteristic parameter set; after a time interval is set, collecting the real-time culture characteristic parameter set again, and calculating a culture characteristic deviation vector based on the real-time culture characteristic parameter set and the reference culture characteristic parameter set; and on the basis of the culture characteristic deviation vector, judging the metabolic state characteristic of the current dendrobium protocorm cell.

The utility model discloses a kind of light regulation devices for Dendrobium huoshanense, applied in Dendrobium planting field, including fixed column, the surface of fixed column is fixed with lampshade, the inner wall cover of lampshade is equipped with glass plate, the surface of fixed column is rotatably connected with rotating ring;The utility model makes gear ring drive first gear to rotate by rotating rotating ring, first gear rotation drives second gear to make screw rod rotate, screw rod rotation makes telescopic link drive lamp head to move, cooperate lampshade, to reach the purpose of adjusting illumination range, reduce use cost, improve practicality and applicability, the utility model is by moving pull block and makes the clamping rod and adjusting hole disengage clamping, after adjusting lamp head to the angle required, loosen pull block, clamping rod is clamped with adjusting hole under the action of spring, its fixed mode, it can reach the purpose of facilitating the adjustment of illumination angle, reduce workload, improve the efficiency of adjustment.

The invention relates to the technical field of slicing machines, and discloses dendrobium huoshanense low-temperature vibration type precise slicing equipment which comprises a low-temperature box arranged at the top of a workbench, the retention rate of medicinal components is increased, working assemblies comprise a movement table, a driving assembly and a vibration assembly, and transmission is achieved through a rotating disc, a connecting gear, a connecting rod and the like; a motor drives a driving roller and a rotating seat to rotate, and convex seats on the rotating seat are in sliding contact with cambered surfaces of connecting seats on the two sides of the moving table, so that the moving table is subjected to high-frequency micro-vibration. The micro-vibration can timely vibrate away fibers and colloids possibly winding a cutter, the low-temperature environment is matched for restraining colloid melting, the shutdown cleaning requirement is reduced, the low-temperature environment, reciprocating cutting and high-frequency micro-vibration are organically combined, the low-temperature environment, reciprocating cutting and high-frequency micro-vibration are organically combined, low-temperature colloid melting restraining and high-frequency micro-vibration winding prevention are achieved, and the clamping stagnation probability is greatly reduced through cooperation of multiple links.

The utility model discloses a dendrobium huoshanense three-dimensional planting frame which is applied to the field of planting frames and comprises a frame body, four fixing cylinders are connected to the bottom of the frame body in a bolting mode, nuts are embedded in the fixing cylinders, first screws are connected to the interiors of the nuts in a threaded mode, the bottoms of the first screws are rotationally connected with a base through bearings, and the bases are connected with the base through bearings. The device comprises a frame body, three planting boxes are arranged on each of the two sides of the frame body, bearing plates are symmetrically arranged at the bottoms of the planting boxes and installed on the frame body, a second screw is connected to one side of each bearing plate in a penetrating and threaded mode, a clamping plate is rotationally connected to one end of each second screw, and limiting blocks are symmetrically installed on the two sides of the frame body. The device has the advantages that the bottom horizontal plane adjusting function can be achieved, uneven internal distribution caused by product inclination is avoided, the stability of the device after being placed is improved, the flexibility is good, meanwhile, the planting box can be limited, the operation is simple and convenient, displacement and falling caused by accidental touch are avoided, and the safety and practicability are high.

This invention relates to the field of plant polysaccharide processing technology, and discloses a highly efficient enzymatic hydrolysis and oligosaccharide detection method for Dendrobium huoshanense polysaccharides. The method involves steps such as drying, impurity removal, pulverization, ethanol defatting, hot water extraction, vacuum concentration, alcohol precipitation, and Sevag deproteinization to obtain refined polysaccharides. These are then subjected to enzymatic hydrolysis. Factors such as hydrolysis time, enzyme dosage, and number of hydrolysis cycles are investigated, and the process is optimized using response surface methodology, significantly increasing the yield of Dendrobium huoshanense oligosaccharides to over 90%. Finally, HPLC-ELSD is used to detect the oligosaccharide composition. This method is highly efficient, simple, and pollution-free, providing high-quality raw materials for the development of Dendrobium huoshanense oligosaccharides and significantly increasing its extraction efficiency and economic value.

This invention discloses the application of Dendrobium huoshanense protocorms in anti-fatigue and kidney-tonifying and aphrodisiac effects. Combining Dendrobium huoshanense protocorms with Cistanche deserticola, American ginseng, Rubus idaeus, Cornus officinalis, and Lycium barbarum significantly enhances the anti-fatigue and kidney-tonifying and aphrodisiac effects of the formula. Compared with Cistanche deserticola alone, adding Dendrobium huoshanense protocorms significantly prolongs the exhaustive swimming time in mice, reduces serum urea nitrogen and lactic acid levels, increases liver glycogen content, and increases muscle glycogen content. Compared with Cistanche deserticola alone, adding Dendrobium huoshanense protocorms significantly increases serum superoxide dismutase activity, reduces serum malondialdehyde content, increases serum testosterone content, increases penile nitric oxide synthase activity, and increases penile nitric oxide and cyclic guanosine monophosphate content in penile tissue.

The application discloses a glycosyltransferase coded by Dendrobium huoshanense UGT70 gene and application of the glycosyltransferase in synthesis of antioxidant flavone disglycosides, and belongs to the technical field of biotechnology. The UGT70 gene nucleotide sequence codes an amino acid sequence as shown in SEQ ID NO. 2. The flavone disglycosides are obtained by using the UGT70 protein to perform enzyme catalysis in vitro by a biosynthesis method, and a new method for synthesizing the flavone disglycosides is provided.

The invention discloses a processing method for improving suspension property and anti-inflammatory activity of dendrobium huoshanense powder, and belongs to the technical field of traditional Chinese medicine processing. The processing method comprises the following steps: mixing dendrobium huoshanense and yellow rice wine, moistening, steaming, and carrying out vacuum drying to obtain the dendrobium huoshanense wine steamed product. Compared with the dendrobium huoshanense processed by a traditional dry strip and maple method, the dendrobium huoshanense wine steamed product obtained by the processing method disclosed by the invention has the advantages that the water solubility of the dendrobium huoshanense is remarkably improved, and the immune function and the anti-inflammatory ability are remarkably enhanced. The complete dendrobium huoshanense wine processing technology is developed and formulated, the difficulty that dendrobium huoshanense powder is inconvenient to take is solved while the biological activity of dendrobium huoshanense is improved, and a new direction is provided for dendrobium huoshanense development.

The invention discloses a promoter of dendrobium huoshanense bibenzyl synthetase DsBBS2. The nucleotide sequence of the DsBBS2 promoter is as shown in any one of SEQ ID NO: 1 to SEQ ID NO: 6. The DsBBS2 promoter is a promoter sequence of dendrobium huoshanense bibenzyl synthetase and can effectively drive expression of an exogenous gene in plant leaves, the invention further provides a method for driving expression of the exogenous gene in a transgenic plant on the basis of the DsBBS2 promoter, the transgenic plant is constructed by using a biological material containing the promoter DsBBS2, and the transgenic plant is constructed by using a biological material containing the promoter DsBBS2. And then DsBBS is used for driving the expression of an exogenous gene.

The invention provides an outdoor greenhouse dendrobium huoshanense seed fungus direct seeding breeding method which comprises the following steps: mixing pine barks, peat soil and perlite to obtain a substrate, and bagging to obtain a substrate bag; carrying out moist heat sterilization on the matrix bag, and adding symbiotic fungi into the matrix bag to obtain a strain bag; after a strain bag is cultivated, substances in the strain bag are laid on a seedbed in a greenhouse, the temperature in the greenhouse is 20-30 DEG C, seeds and saw dust are mixed and sown on the substances in the strain bag, then water is supplemented, a planting area is formed, symbiotic fungus liquid is supplemented to the planting area once every month, and breeding is completed until the seeds germinate three leaves. According to the method, the symbiotic fungi are soaked in the matrix in advance, so that the survival of the symbiotic fungi can be effectively improved, the seed infection speed and the germination rate are improved, the germination time is advanced by more than 10 days, and the germination rate is improved to more than 40%.

The utility model discloses a squeezing device for producing dendrobium huoshanense juice, and relates to the technical field of squeezing devices, the squeezing device comprises two first guide rails, a second guide rail is arranged outside each first guide rail, a connecting mechanism is arranged outside each first guide rail, each connecting mechanism comprises a groove, an inner groove, a rotating shaft, a connecting block, a first spring, a fixing block and a fixing groove, by arranging a fixing block and cooperating with a second guide rail, when the squeezing device is moved, the fixing block is pressed to release connection between the fixing block and a fixing groove, at the moment, the second guide rail can be pulled to be away from the first guide rail, and at the moment, limitation of a baffle to a connecting block is released; at the moment, the baffle is moved to be attached to the surface of the second guide rail, at the moment, the second guide rail cannot rotate downwards, the guide rail part located outside the machine body is designed to be rotatable, the guide rail part can be rotated upwards during transportation, and the overall occupied space of the equipment is compressed.

The present invention discloses a dendrobium huoshanense and American ginseng special diet, and relates to the technical field of traditional Chinese medicine diets, and the dendrobium huoshanense and American ginseng special diet comprises the following raw materials in parts by mass: 1-3 parts of dendrobium huoshanense, 9-11 parts of Chinese wolfberry fruits, 9-11 parts of radix astragali, 4-6 parts of American ginseng, 4-6 parts of radix ophiopogonis and 149-151 parts of inulin. According to the method, the retention rate of thermosensitive components such as dendrobium huoshanense polysaccharide is increased from 60-68% to 85% or above through vacuum concentration at 55-60 DEG C, and high-temperature degradation is avoided; the integration of mixing, spraying and drying is realized by adopting a one-step granulation technology, the granulation period is shortened to be within 1.5 hours, and the particle yield is improved by 15-20%; a bag-containing total weight control method is innovatively introduced, the batch fluctuation of packaging materials is effectively compensated, the packaging quantity difference is stabilized within + / -5% and is far better than the + / -10% standard of traditional net weight control, the whole process achieves industrialization upgrading of low energy consumption, high stability and high quality control, and the active ingredient retention rate, the production efficiency and the packaging precision are remarkably improved.

The invention relates to the technical field of food beverages, and discloses a dendrobium huoshanense soda water beverage and a preparation method thereof.The dendrobium huoshanense soda water beverage is prepared from, by weight, 40-70 parts of a dendrobium huoshanense extracting solution, 1.5-3.5 parts of beta-cyclodextrin potassium, 15-35 parts of citric acid, 0.3-1.2 parts of zinc bicarbonate, 0.1-0.6 part of mogroside and 850-950 parts of purified water; the preparation method of the dendrobium huoshanense soda water beverage comprises the following steps: S1, performing enzymolysis extraction on dendrobium huoshanense; s2, carrying out beta-cyclodextrin embedding treatment on the dendrobium huoshanense extracting solution; s3, preparing a buffer solution; s4, compounding and mixing all the components; and S5, filling and sterilizing the beverage. The dendrobium huoshanense extract and beta-cyclodextrin embedding technology is adopted, the stability of active ingredients in the beverage is successfully improved, and the damage of the external environment to the ingredients is reduced by accurately controlling the embedding mode of the extract.

The application discloses a Dendrobium huoshanense protocorm functional food with an enhanced immunity effect, which is prepared by compounding Dendrobium huoshanense protocorm extract, double-spore mushroom extract and Raphiolepis umbellate leaf extract according to a specific weight ratio. The Dendrobium huoshanense protocorm functional food with the enhanced immunity effect prepared by the application can significantly enhance the cell immunity function, the humoral immunity function, the monocyte-macrophage function and the NK cell activity of test animals through animal test verification.

The application discloses anti-inflammatory toothpaste based on Dendrobium huoshanense stem extract and a preparation method thereof, and relates to the technical field of toothpaste.The anti-inflammatory toothpaste contains the following raw materials in parts by weight: friction agent 20-30 parts, humectant 15-30 parts, emulsifier 1-2 parts, thickening agent 1-2.5 parts, Dendrobium huoshanense stem extract 1-3 parts, Abelmoschus flower extract 0.5-1.5 parts, Perilla leaf extract 0.5-1.5 parts, anti-allergic agent 3.5-7.5 parts, auxiliary material 1-3.5 parts, and deionized water 20-40 parts.The specific plant extract and the Dendrobium huoshanense stem extract are synergistically used, and the basic formula system is optimized, so that the comprehensive anti-inflammatory effect of the product is effectively improved, and the stability and mildness of the toothpaste are improved.

The invention discloses a processing method and processing equipment based on dendrobium huoshanense leaves, and relates to the technical field of agricultural product processing, raw material pretreatment: selecting fresh dendrobium huoshanense leaves (within 24 hours after picking), and removing yellow leaves, residual leaves and impurities; cleaning and processing equipment is adopted for cleaning, and flowing clear water is used for flushing for 6-7 times. According to the processing method and the processing equipment based on the dendrobium huoshanense leaves, aiming at the characteristics of the dendrobium huoshanense leaves, key parameters such as fixation temperature, stir-frying time and mode, carbon culture time and the like of the Liuan Guapian tea are optimized, and compared with a common stir-frying process, the retention rate of polysaccharide in a finished product is increased by 20%-25%, and the retention rate of alkaloid is increased by 15%-18%; the bitter and astringent taste of the leaves is removed through the technology, the fishy smell is eliminated, the soup color is yellow green, bright and clear, the finished product is endowed with unique scorch aroma and the original dendrobium huoshanense flavor mixed aroma, the taste is mellow and sweet after taste, the medicinal value and palatability are considered, and the market requirements of medicinal and edible products are met.