The invention discloses a preparation method of a dendrobium huoshanense wine for improving human body immunity. The dendrobium huoshanense wine is prepared from distilled spirit taking as base liquor and traditional Chinese medicinal materials, wherein the traditional Chinese medicinal materials comprise dendrobium huoshanense, desertliving cistanche, American ginseng, herb of shorthorned epimedium, barbary wolfberry fruit, prepared rehmannia root, pulp of cornus, eucommia bark, Chinese taxillus twig, oriental waterplantain rhizome, cassia bark, tuber onion seed, hippocamp, cornu cervi pantotrichum and licorice. The preparation method comprises the following steps: crushing the medicinal materials into fine powder of 80-120 meshes, adding 5-8 times of the white spirit of 55-60 degrees, mixing and soaking for 8-12 days, and filtering out wine liquid; and adding 3-5 times of the white spirit of 55-60 degrees, soaking for 6-9 days continuously, filtering out wine liquid, combing the wine liquids, and mixing to an appropriate wine degree to obtain the dendrobium huoshanense wine. The dendrobium huoshanense wine disclosed by the invention has the effects of reinforcing kidney to strengthen yang, replenishing qi to strengthen essence and keeping fit, and has very good curative effects on hyposexuality, cold semen weakness, lassitude in loin and legs, dysphoria insomnia, chronic prostatitis and the like caused by insufficiency of kidney-yang and deficiency of qi and blood.