52 results about "Dendrobium huoshanense" patented technology

The invention relates to a Tulasnella sp. QYM-1, a culture method of dendrobium huoshanense and application of the Tulasnella sp. QYM-1, and belongs to the technical field of microorganisms. The invention provides the mucilaginoidea fungus QYM-1, and the mucilaginoidea fungus QYM-1 is separated from protocorm formed by symbiotic germination, can promote germination of dendrobium huoshanense seeds to form seedlings, has relatively strong specificity on culture of the dendrobium huoshanense seeds, can replace tissue culture to become a main technology for seedling culture of dendrobium huoshanense, and has a broad application prospect in the field of seedling culture of dendrobium huoshanense. And the method has extremely high application value in production. In addition, the mucilaginoidea fungus QYM-1 is low in production cost and suitable for large-area popularization and application, and has huge research potential in the aspects of regression of rare and endangered orchid plants, wild-imitating cultivation of medicinal orchid plants and the like.

1. The name of the design product: beverage bottle (winding bottle). 2. The use of the design product: the design product is used for beverage bottles. 3. The design points of the design product: the shape of the product, the design is derived from the spiral shape of the Dendrobium huoshanense, the spiral-shaped ribs on the bottle body. 4. The picture or photo that best indicates the design points: design 1 perspective view. 5. Design 1 is designated as the basic design.

The invention provides freeze-dried fresh dendrobium huoshanense powder and a preparation process thereof, and relates to the technical field of freeze-dried fresh dendrobium huoshanense powder. The preparation process of the freeze-dried fresh dendrobium huoshanense powder comprises the following steps: S1, material selection: selecting and taking dendrobium huoshanense as a raw material, S2, cleaning: cleaning and drying the raw material in the S1, S3, cutting: cutting the raw material in the S2, S4, crushing and pulping: pulping the raw material in the S3 and mixing the pulped raw material with water, S5, stirring and mixing: stirring the pulp in the S4, and performing freeze-drying to obtain the freeze-dried fresh dendrobium huoshanense powder. And S6, freeze-drying and crushing: freeze-drying the slurry obtained in the step S5, and crushing the freeze-dried slurry into powder. The dendrobium huoshanense freeze-dried fresh powder is cut, pulped and freeze-dried and crushed, so that the dendrobium huoshanense freeze-dried fresh powder is finer and smaller in particle size, tastes better when being eaten by old people, is improved in taste and is convenient for the old people to eat.

The invention relates to the technical field of dendrobium huoshanense processing equipment, in particular to a dendrobium huoshanense low-temperature wall-breaking grinding device which comprises a shell; the crushing and grinding unit is connected with the shell; collecting subrack boxes; the cooling unit is connected with the shell; wherein the smashing and grinding unit comprises a smashing and screening assembly, a material guiding assembly, a transmission control assembly and a grinding assembly, the smashing and screening assembly is fixedly connected with the shell wall of the top end of the shell, the grinding assembly is arranged between the smashing and screening assembly and the inserted collection insertion box, and the grinding assembly is slidably connected with the shell. A material guiding assembly is arranged between the grinding assembly and the smashing and screening assembly, the material guiding assembly is in sliding connection with the inner wall of the shell and connected with a transmission control assembly arranged on the shell, and the transmission control assembly is connected with the smashing and screening assembly. And the grinding efficiency and quality are greatly improved.

The invention discloses a dendrobium huoshanense extract and application of the dendrobium huoshanense extract in preparation of a medicine for preventing and / or treating depression, and belongs to the technical field of biological medicine. The dendrobium huoshanense extract is obtained through reflux extraction by using an alcohol-water mixed solution, and the volume concentration of alcohol in the alcohol-water mixed solution is 50%-90%. The reflux extraction conditions are as follows: the temperature is 70-90 DEG C, and the time is 1-4 hours. According to behavioral data analysis of mouse sweet water preference, tail suspension test, forced swimming test, open field test, elevated cross labyrinth test and the like, the dendrobium huoshanense extract has a remarkable anti-depression effect, has a good effect of preventing and / or treating depression, and has a good application prospect in prevention and / or treatment of depression.

The invention discloses application of Dendrobium huoshanense DhOMT8 gene coded methyltransferase in biosynthesis of a flavonoid compound for regulating gastrointestinal activity, and belongs to the technical field of biology. The nucleotide sequence of the DhOMT8 gene encodes a nucleotide sequence of an amino acid sequence as shown in SEQ ID NO. 2. The methylated flavonoid compound is obtained by a biological synthesis method for carrying out enzyme catalysis by using the DhOMT8 protein in vitro, and a new method is provided for synthesizing the flavonoid compound for regulating gastrointestinal activity.

The invention discloses a mucilaginosa N15 and a method for promoting the germination of dendrobium huoshanense seeds. The mucilaginosa N15 is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on October 27, 2025, and the preservation number of the strain is CGMCC No: 42290. The invention further discloses a method for promoting the germination of dendrobium huoshanense seeds by using the mucilaginosa N15 and the method for promoting the germination of dendrobium huoshanense seeds by using the mucilaginosa N15. The strain can be prepared into a high-activity microbial agent after activation and amplification culture, and the high-activity microbial agent is mixed with dendrobium huoshanense seeds and then sown in a greenhouse, so that the seed germination efficiency can be remarkably improved. By applying the method, the germination rate of the dendrobium huoshanense seeds can be increased to 60% or above, the mycorrhiza infection rate reaches 80% or above, the comprehensive cost is reduced by 30%, the seedling transplanting survival rate reaches 90% or above, and the seedling raising period is shortened to 12-15 months. The method is easy and convenient to operate, low in cost, high in transplanting survival rate and suitable for large-scale seedling raising and resource protection of dendrobium huoshanense, and has important economic and ecological values.

The invention discloses dendrobium huoshanense neutral polysaccharide as well as a preparation method and application thereof, and relates to the technical field of traditional Chinese medicinal material processing, the dendrobium huoshanense neutral polysaccharide has Mn of 5-15 * 10 < 3 > Da and MW of 5-15 * 10 < 3 > Da, monosaccharide comprises arabinose (Ara), glucose (Glc) and mannose (Man) in a molar mass ratio of (0.5-1.0%): (25-30%): (69-74.5%), and the purity is greater than 90%; the determination of molecular weight and the analysis of monosaccharide composition of the dendrobium huoshanense neutral polysaccharide show that the dendrobium huoshanense neutral polysaccharide has an obvious promotion effect on the proliferation of animal bifidobacterium in a specific concentration range (0.25%-0.5%), is added into food or developed into health-care food for regulating the intestinal flora function, and has an obvious promotion effect on the proliferation of animal bifidobacterium. The proliferation of bifidobacteria can be effectively promoted, the micro-ecological balance of intestinal tracts is improved, the health of human bodies is maintained, and wide development potential and wide market prospects are achieved.

The invention discloses application of glycosyl transferase coded by a dendrobium huoshanense DhUGT44 gene in biosynthesis of cosmosiin, and belongs to the technical field of biology. The nucleotide sequence of the DhUGT44 gene encodes a nucleotide sequence of an amino acid sequence as shown in SEQ ID NO. 2. The cosmosiin is obtained by a biosynthesis method for carrying out enzyme catalysis by utilizing the DhUGT44 protein in vitro, and a new method is provided for synthesizing the cosmosiin.

The invention discloses dendrobium huoshanense compound granules as well as a preparation method and a quality detection method thereof, and belongs to the technical field of traditional Chinese medicine preparations. Dendrobium huoshanense, radix rehmanniae recen, radix ophiopogonis and liquorice are used as a formula, granules are selected as a medicine carrying form, the advantages of maintaining property and flavor tropism of each medicine, mainly treating functions, being relatively large in medicine carrying capacity, easy to dissolve, quick in effect taking and simple and convenient in process are exerted, and a decoction process, a concentration drying process and a granulation process in pharmacy are optimized; the preparation method which is simple and convenient in granulation, high in yield and high in stability is obtained, and the obtained compound granules are uniform in granules, small in angle of repose and relatively good in flowability. Quality control experiment results show that the dendrobium huoshanense compound granules prepared according to the preparation method provided by the invention are high in content of effective components such as total flavonoids and rehmannia D, the process is stable, and technical support is provided for treatment of oral mucositis.

The invention discloses a dendrobium huoshanense compound traditional Chinese medicine composition for treating dysmenorrhea and a preparation method of the dendrobium huoshanense compound traditional Chinese medicine composition, and belongs to the field of traditional Chinese medicine compounds. The dendrobium huoshanense compound traditional Chinese medicine composition for treating dysmenorrhea comprises 6-12 parts of dendrobium huoshanense, 9-15 parts of glossy privet fruit, 12-18 parts of radix paeoniae alba, 9-15 parts of dogwood, 9-15 parts of radix rehmanniae recen, 6-12 parts of cortex lycii radicis, 9-15 parts of teasel root, 6-12 parts of eucommia ulmoides, 3-9 parts of honey-fried licorice root and 6-12 parts of achyranthes bidentata. The invention also provides a preparation method of the composition. Compared with the prior art, the prepared dendrobium huoshanense compound traditional Chinese medicine composition for treating dysmenorrhea has the advantage of being good in effect of treating dysmenorrhea caused by deficiency of liver and kidney, yin deficiency and internal heat.

The invention relates to the technical field of transgenosis, and discloses a method for transgenosis by using dendrobium huoshanense protocorm, which comprises the following steps: S1, induction culture of dendrobium huoshanense protocorm; s2, constructing a plant expression vector; s3, infecting and culturing the dendrobium huoshanense protocorm by agrobacterium tumefaciens; s4, screening and culturing the dendrobium huoshanense transgenic tissue and seedlings, the dendrobium huoshanense protocorm is used as a material, agrobacterium tumefaciens-mediated genetic transformation is realized on the basis of a dendrobium huoshanense protocorm tissue rapid propagation system, a stable and rapid genetic transformation system of dendrobium huoshanense is established, and creation of a new variety of dendrobium huoshanense is realized.

The invention discloses a preparation method and application of a dendrobe salt roasted product, and belongs to the technical field of traditional Chinese medicines. The preparation method comprises the following steps: drying fresh dendrobium huoshanense strips serving as a raw material, adding saline water for moistening until the core of the dendrobium huoshanense is completely penetrated, and frying to obtain the salt roasted dendrobium huoshanense product. According to the preparation method, a single factor is combined with a Box-Behnken response surface method, the contents of main medicinal components including total polysaccharides, total flavonoids, alcohol-soluble extracts and water-soluble extracts of dendrobium huoshanense are taken as evaluation indexes, comprehensive scoring is carried out by combining an entropy weight method, and the preparation method of the dendrobium huoshanense salt roasted product is obtained through optimization. The quality of the dendrobium huoshanense salt honey-fried product obtained by the method is obviously improved, the total polysaccharide content and the alcohol-soluble extract content of the dendrobium huoshanense salt honey-fried product exceed 12.82% and 51.25% in the specification of pharmacopeia respectively, and the effect of improving hyperthyroidism yin deficiency is also obviously improved.

The invention provides a preparation method of uric acid reducing microsphere particles containing a dendrobium huoshanense leaf extract, and belongs to the technical field of uric acid reducing compositions.The specific method includes the steps that fresh dendrobium huoshanense leaves are subjected to alcohol extraction to obtain a dendrobium huoshanense leaf ethanol extracting solution, and then the dendrobium huoshanense leaf ethanol extracting solution is purified through an AB-8 resin chromatographic column to obtain the uric acid reducing microsphere particles containing the dendrobium huoshanense leaf extract. The brand-new natural dendrobium huoshanense leaf extract microsphere particle for reducing uric acid is obtained by combining a corn polypeptide prolamin mixed solution formed by mixing a corn prolamin solution and a corn polypeptide solution, has the effect of remarkably reducing uric acid of mice, and has the advantages that the stability of effective components is kept after the particle is stored for 1-2 months, and the bioavailability of the particle is improved. The uric acid value of a hyperuricemia mouse can be obviously reduced, and the economic value is very good.

The invention belongs to the technical field of microorganisms, and discloses a Tulasnella sp. Strain and application of the Tulasnella sp. Strain. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) The strain has a remarkable promoting effect on seed germination, seedling growth, seedling rooting and accumulation of polysaccharide content in stems of dendrobium officinale and dendrobium huoshanense.

1. The name of this design product: Packaging box (Huoshan Dendrobium). 2. Purpose of this design product: used for packaging of Chinese medicinal materials. 3. The key design points of this product are the combination of shape, pattern and color. 4. The picture or photo that best illustrates the design points: three-dimensional picture. 5. The design for which protection is sought includes color.

1.The name of the design product: packaging box (special for Dendrobium huoshanense). 2.The use of the design product: the design product is used for the outer packaging of Dendrobium huoshanense. 3.The design points of the design product: the combination of shape, pattern and color. 4.The picture or photo that best shows the design points: perspective view. 5.The design claimed contains color.

The invention discloses a dendrobium huoshanense UGT70 gene coded glycosyl transferase and application thereof in synthesis of antioxidant flavonoid bioglycosides, and belongs to the technical field of biology. Nucleotide sequence coding of the UGT70 gene is an amino acid sequence as shown in SEQ ID NO. 2. The flavonoid diglucoside compound is obtained by a biosynthesis method for carrying out enzyme catalysis by utilizing UGT70 protein in vitro, and a new method is provided for synthesizing the flavonoid diglucoside compound.

The invention relates to the technical field of tissue culture, in particular to a tissue culture control method for increasing the content of polysaccharide in protocorm of dendrobium huoshanense, the method is carried out in a bioreactor provided with an online monitoring system, and the online monitoring system is at least used for monitoring a culture characteristic parameter set of a culture solution in real time; the method comprises the following steps: culturing dendrobium huoshanense protocorm under a first culture condition suitable for cell proliferation; judging whether cell growth enters a proliferation saturation state or not based on a culture characteristic parameter set monitored by an online monitoring system in real time; if yes, triggering a polysaccharide induction stage, and taking the culture characteristic parameter set for triggering the polysaccharide induction stage as a reference culture characteristic parameter set; after a time interval is set, collecting the real-time culture characteristic parameter set again, and calculating a culture characteristic deviation vector based on the real-time culture characteristic parameter set and the reference culture characteristic parameter set; and on the basis of the culture characteristic deviation vector, judging the metabolic state characteristic of the current dendrobium protocorm cell.

The utility model discloses a kind of light regulation devices for Dendrobium huoshanense, applied in Dendrobium planting field, including fixed column, the surface of fixed column is fixed with lampshade, the inner wall cover of lampshade is equipped with glass plate, the surface of fixed column is rotatably connected with rotating ring;The utility model makes gear ring drive first gear to rotate by rotating rotating ring, first gear rotation drives second gear to make screw rod rotate, screw rod rotation makes telescopic link drive lamp head to move, cooperate lampshade, to reach the purpose of adjusting illumination range, reduce use cost, improve practicality and applicability, the utility model is by moving pull block and makes the clamping rod and adjusting hole disengage clamping, after adjusting lamp head to the angle required, loosen pull block, clamping rod is clamped with adjusting hole under the action of spring, its fixed mode, it can reach the purpose of facilitating the adjustment of illumination angle, reduce workload, improve the efficiency of adjustment.

The application discloses anti-inflammatory toothpaste based on Dendrobium huoshanense stem extract and a preparation method thereof, and relates to the technical field of toothpaste.The anti-inflammatory toothpaste contains the following raw materials in parts by weight: friction agent 20-30 parts, humectant 15-30 parts, emulsifier 1-2 parts, thickening agent 1-2.5 parts, Dendrobium huoshanense stem extract 1-3 parts, Abelmoschus flower extract 0.5-1.5 parts, Perilla leaf extract 0.5-1.5 parts, anti-allergic agent 3.5-7.5 parts, auxiliary material 1-3.5 parts, and deionized water 20-40 parts.The specific plant extract and the Dendrobium huoshanense stem extract are synergistically used, and the basic formula system is optimized, so that the comprehensive anti-inflammatory effect of the product is effectively improved, and the stability and mildness of the toothpaste are improved.

The invention provides a dendrobium huoshanense seedling hardening device, and relates to the technical field of dendrobium huoshanense seedling hardening. Comprising a seedling hardening temperature control room, a cultivation moving plate, a water tank and a collecting tank, a flow guide plate is arranged at the bottom end of the seedling hardening temperature control room, the cultivation moving plate is inserted above the flow guide plate, groove blocks are fixedly connected to the two sides of the bottom end of the cultivation moving plate, and rolling wheels are rotationally matched with the interiors of the groove blocks; the rollers are rotatably matched with the inner wall of the seedling hardening temperature control room, a plurality of cultivation tanks are arranged above the cultivation moving plate, a water tank is fixedly connected to the bottom end of the rear side of the seedling hardening temperature control room, and first water pumps are fixedly connected to the two sides of the top end of the water tank. By arranging a first water pump, a water tank, a spraying nozzle, a second motor and a spoiler, the first water pump pumps out a water source in the water tank, circulation is achieved through a water pipe, the spraying nozzle completes atomization spraying, and the humidity in the seedling hardening temperature control room can be increased in the water source spraying process.

The invention relates to the technical field of slicing machines, and discloses dendrobium huoshanense low-temperature vibration type precise slicing equipment which comprises a low-temperature box arranged at the top of a workbench, the retention rate of medicinal components is increased, working assemblies comprise a movement table, a driving assembly and a vibration assembly, and transmission is achieved through a rotating disc, a connecting gear, a connecting rod and the like; a motor drives a driving roller and a rotating seat to rotate, and convex seats on the rotating seat are in sliding contact with cambered surfaces of connecting seats on the two sides of the moving table, so that the moving table is subjected to high-frequency micro-vibration. The micro-vibration can timely vibrate away fibers and colloids possibly winding a cutter, the low-temperature environment is matched for restraining colloid melting, the shutdown cleaning requirement is reduced, the low-temperature environment, reciprocating cutting and high-frequency micro-vibration are organically combined, the low-temperature environment, reciprocating cutting and high-frequency micro-vibration are organically combined, low-temperature colloid melting restraining and high-frequency micro-vibration winding prevention are achieved, and the clamping stagnation probability is greatly reduced through cooperation of multiple links.

The utility model discloses an insect-proof dendrobium huoshanense planting shed, which is applied to the field of planting sheds and comprises two bases, and an arched support is arranged between the two bases. The first reinforcing rods and the second reinforcing rods are installed among the multiple arched supports, the first reinforcing supporting rods are installed between the first reinforcing rods and the second reinforcing rods, and the connecting rods are installed between the two first reinforcing supporting rods, so that the compression resistance of the arched supports of the planting shed can be improved; the situation that the arched supports are collapsed due to heavy snow weather is prevented, the compression resistance of the arched supports is improved, the water collecting grooves are designed in the two sides of the planting shed and matched with the water guide pieces for use, rainwater falling from the planting shed can be collected in rainy weather, the rainwater collected in the water collecting grooves can be used for subsequent irrigation, and the planting shed is convenient to use. And the amount of external water is reduced, so that the aim of saving water resources is fulfilled.

The utility model discloses a dendrobium huoshanense three-dimensional planting frame which is applied to the field of planting frames and comprises a frame body, four fixing cylinders are connected to the bottom of the frame body in a bolting mode, nuts are embedded in the fixing cylinders, first screws are connected to the interiors of the nuts in a threaded mode, the bottoms of the first screws are rotationally connected with a base through bearings, and the bases are connected with the base through bearings. The device comprises a frame body, three planting boxes are arranged on each of the two sides of the frame body, bearing plates are symmetrically arranged at the bottoms of the planting boxes and installed on the frame body, a second screw is connected to one side of each bearing plate in a penetrating and threaded mode, a clamping plate is rotationally connected to one end of each second screw, and limiting blocks are symmetrically installed on the two sides of the frame body. The device has the advantages that the bottom horizontal plane adjusting function can be achieved, uneven internal distribution caused by product inclination is avoided, the stability of the device after being placed is improved, the flexibility is good, meanwhile, the planting box can be limited, the operation is simple and convenient, displacement and falling caused by accidental touch are avoided, and the safety and practicability are high.

This invention relates to the field of plant polysaccharide processing technology, and discloses a highly efficient enzymatic hydrolysis and oligosaccharide detection method for Dendrobium huoshanense polysaccharides. The method involves steps such as drying, impurity removal, pulverization, ethanol defatting, hot water extraction, vacuum concentration, alcohol precipitation, and Sevag deproteinization to obtain refined polysaccharides. These are then subjected to enzymatic hydrolysis. Factors such as hydrolysis time, enzyme dosage, and number of hydrolysis cycles are investigated, and the process is optimized using response surface methodology, significantly increasing the yield of Dendrobium huoshanense oligosaccharides to over 90%. Finally, HPLC-ELSD is used to detect the oligosaccharide composition. This method is highly efficient, simple, and pollution-free, providing high-quality raw materials for the development of Dendrobium huoshanense oligosaccharides and significantly increasing its extraction efficiency and economic value.

This invention discloses the application of Dendrobium huoshanense protocorms in anti-fatigue and kidney-tonifying and aphrodisiac effects. Combining Dendrobium huoshanense protocorms with Cistanche deserticola, American ginseng, Rubus idaeus, Cornus officinalis, and Lycium barbarum significantly enhances the anti-fatigue and kidney-tonifying and aphrodisiac effects of the formula. Compared with Cistanche deserticola alone, adding Dendrobium huoshanense protocorms significantly prolongs the exhaustive swimming time in mice, reduces serum urea nitrogen and lactic acid levels, increases liver glycogen content, and increases muscle glycogen content. Compared with Cistanche deserticola alone, adding Dendrobium huoshanense protocorms significantly increases serum superoxide dismutase activity, reduces serum malondialdehyde content, increases serum testosterone content, increases penile nitric oxide synthase activity, and increases penile nitric oxide and cyclic guanosine monophosphate content in penile tissue.

The invention provides a preparation process of a dendrobium huoshanense concentrated solution suitable for a mask, and relates to the field of preparation of dendrobium huoshanense concentrated solutions. The preparation process of the dendrobium huoshanense concentrated solution suitable for the facial mask comprises the following steps: S1, cleaning materials: cleaning and drying dry dendrobium huoshanense stems, S2, cutting and crushing: cutting the dry dendrobium huoshanense stems in the S1, and performing crushing treatment through a crusher, S3, cooking the materials, S4, performing low-temperature treatment: performing low-temperature condensation treatment, and S5, preparing the dendrobium huoshanense concentrated solution suitable for the facial mask. S5, adding ethanol: adding an ethanol solution in the step S4, S6, performing ultrafiltration treatment, and S7, performing blending. According to the dendrobium huoshanense concentrated solution mask, materials are cut and smashed, ethyl alcohol is added, the release amount of effective substances in dry dendrobium huoshanense stems is conveniently increased, the use effect of the dendrobium huoshanense concentrated solution mask is improved, a concentrated solution is filtered through an ultrafiltration membrane, impurities in the concentrated solution are conveniently filtered, and the cleanliness of the concentrated solution is improved.

The application discloses a glycosyltransferase coded by Dendrobium huoshanense UGT70 gene and application of the glycosyltransferase in synthesis of antioxidant flavone disglycosides, and belongs to the technical field of biotechnology. The UGT70 gene nucleotide sequence codes an amino acid sequence as shown in SEQ ID NO. 2. The flavone disglycosides are obtained by using the UGT70 protein to perform enzyme catalysis in vitro by a biosynthesis method, and a new method for synthesizing the flavone disglycosides is provided.

The invention relates to the technical field of plant cultivation, in particular to a dendrobium huoshanense stem cell cloning proliferation control method, which improves the proliferation efficiency and quality and enhances the resource protection effect. The method comprises the following steps: acquiring a target proliferation characteristic quantization set of dendrobium huoshanense stem cell clone proliferation for each preset culture stage based on a dendrobium huoshanense stem cell clone proliferation design requirement; at the end time node of each preset culture stage, culture environment data of the dendrobium huoshanense stem cells are collected, and a culture environment data set and a real-time proliferation characteristic quantification set are obtained; calculating a deviation distance between the real-time multiplication feature quantization set and the corresponding target multiplication feature quantization set to obtain a real-time multiplication feature deviation vector; and inputting the real-time proliferation characteristic deviation vector and the corresponding culture environment data set into a pre-constructed culture environment regulation and control analysis model to obtain a culture environment regulation and control strategy for the next preset culture stage.