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Method for preparing protein feed by utilizing waste liquid fermentation in production of ginkgo leaf extracts

A technology for fermenting protein feed and extracts, which is applied in the field of preparing protein feed by fermentation of waste liquid in the production of Ginkgo biloba extract, which can solve the problems of environmental pollution, waste of resources, waste, etc. Boosts highly nutritious effects

Inactive Publication Date: 2015-04-01
JIANGSU QIANYAOTANG GUOYI RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, during the production process of Ginkgo biloba extract (GBE), only 0.2-0.425% of the total flavonoids are extracted by macroporous resin, while other organic and inorganic beneficial nutrients are left in the aqueous solution Discharged as waste liquid will not only pollute the environment, but many beneficial ingredients with high nutritional value will be discarded, resulting in a great waste of resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Take the waste liquid that flows out after the Ginkgo flavonoids are absorbed by the macroporous adsorption resin in the production of Ginkgo biloba extract, and concentrate the waste liquid at a temperature of 80°C and a vacuum of -0.1MPa until the water content of the concentrate is reached. at 60-62%;

[0037] (2) Take 82.28 kg of the concentrate obtained in step (1), add 3.08 kg of brown sugar, 2.2 kg of malt extract, and 0.44 kg of yeast extract, stir evenly, and heat press at 0.7 kg / m 2 Sterilize at 115°C for 30 minutes to obtain the production fermentation medium;

[0038] (3) When the sterilized fermentation medium in step (2) is cooled to below 40°C, add a compound microbial starter consisting of 6 kg of Candida, 3 kg of Bacillus subtilis and 3 kg of Streptococcus lactis, Stir evenly, pile the fermented material into a 4-6cm thick material layer, place it in a thick-layer ventilated bioreactor, control the cultivation temperature at 34°C, and the ventilati...

Embodiment 2

[0041] (1) Take the waste liquid that flows out after the Ginkgo flavonoids are absorbed by the macroporous adsorption resin in the production of Ginkgo biloba extract, and concentrate the waste liquid at a temperature of 90°C and a vacuum of -0.065MPa until the water content of the concentrate is reached. at 60-62%;

[0042] (2) Take 87.17 kg of the concentrate obtained in step (1), add 2.3 kg of brown sugar, 2.3 kg of malt extract, and 0.23 kg of yeast extract, stir evenly, and heat press at 0.7 kg / m 2 Sterilize at 115°C for 30 minutes to obtain the production fermentation medium;

[0043] (3) When cooling the fermented medium sterilized in step (2) below 40°C, add a compound microbial starter consisting of 4 kg of Candida, 2 kg of Bacillus licheniformis and 2 kg of Lactobacillus lactis, Stir evenly, pile the fermentation material into a 4-6cm thick material layer, place it in a thick-layer ventilated bioreactor, control the cultivation temperature at 30°C, and the ventilat...

Embodiment 3

[0046] (1) Take the waste liquid that flows out after the Ginkgo flavonoids are absorbed by the macroporous adsorption resin in the production of Ginkgo biloba extract, and concentrate the waste liquid at a temperature of 85°C and a vacuum of -0.08MPa until the water content of the concentrate is reached. at 60-62%;

[0047] (2) Take 84.25 kg of the concentrate obtained in step (1), add 2.67 kg of brown sugar, 1.78 kg of malt extract, and 0.3 kg of yeast extract, stir evenly, and heat press at 0.7 kg / m 2 Sterilize at 115°C for 30 minutes to obtain the production fermentation medium;

[0048] (3) When the fermentation medium sterilized in step (2) is cooled to below 40°C, add a composite microbial starter consisting of 5.5 kg of Saccharomyces cerevisiae, 2.75 kg of Bacillus cereus and 2.75 kg of Lactobacillus bulgaricus, Stir evenly, pile the fermentation material into a 4-6cm thick material layer, place it in a thick-layer ventilated bioreactor, control the cultivation temper...

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Abstract

The invention discloses a method for preparing protein feed by utilizing waste liquid fermentation in production of ginkgo leaf extracts. The method is characterized by comprising the steps: adsorbing ginkgetin through macroreticular resins in the production of ginkgo leaf extracts; condensing effluent waste liquid which is obtained by the adsorption until the water content reaches 60-62 percent; adding concentrates including 2.5-3.5 percent of brown sugars, 1.5-2.5 percent of malt extracts and 0.25-0.5 percent of yeast extracts; performing hot-pressing sterilization; cooling the temperature to be below 40 DEG C; adding 8-12 percent of composite microbiological bacteria leavening agents at the temperature of 30-34 DEG C under the condition of the ventilation quantity of 0.8 m<3> / min to perform solid state fermentation for 60-72 hours; drying in vacuum at the temperature of 50-65 DEG C under the condition that the vacuum degree is -0.07--0.1MPa until the water content is 4-6 percent so as to obtain a fermentation protein feed product. According to the method, a new method, a new technology and a new technical route are provided for development and utilization of waste liquid caused by processing the ginkgo leaf extracts, and the feasibility is provided for effective utilization of wastes; moreover, the fermentation protein feed product is easy and simple in processing, high in utilization rate, low in cost and high in economic benefit, and has a great popularization and application value.

Description

technical field [0001] The invention belongs to the field of feed processing, relates to the field of biological feed processing, in particular to a method for preparing protein feed by fermenting waste liquid in the production of ginkgo biloba extract. Background technique [0002] Ginkgo biloba extract is crushed from Ginkgo biloba leaves, heated and refluxed with ethanol, the extracts are combined, the ethanol is recovered and concentrated to an appropriate amount, added to the treated macroporous adsorption resin column, and eluted with water and different concentrations of ethanol in sequence , collect the corresponding eluate, recover ethanol, and spray dry; or recover ethanol, concentrate into a thick paste, vacuum dry, and pulverize to obtain (National Pharmacopoeia Committee, Pharmacopoeia of the People's Republic of China, 2010 edition, part one, page 392). However, the current manufacturers of Ginkgo biloba extracts have all optimized the extraction process of Gin...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K1/18
Inventor 张奎昌张志年
Owner JIANGSU QIANYAOTANG GUOYI RES INST CO LTD
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