83 results about "Bacillus amylolyticus" patented technology

The invention relates to bacillus amyloliquefaciens NCPSJ7 and further relates to an application of the strain in treating plant diseases, diseases before and after harvesting fruits and vegetables as well as in preventing food-borne pathogenic bacteria and putrefying bacteria. The strain is preserved in China Center for Type Culture Collection (CCTCC) on March 22, 2013, wherein the preservation number is CCTCC NO: M2013098 and the strain is named as bacillus amyloliquefaciens NCPSJ7. The thallus and fermentation liquor of the strain disclosed by the invention has the effects of treating plant diseases caused by plant pathogenic fungus including antagonistic peach root rotten disease, fusarium wilt of cucumber, botrytis cinerea, jujube anthracnose, pear black spot, pear blue mould, apple brown rot, apple altermaria leaf spot, watermelon fusarium wilt and the like, as well as diseases after harvesting fruits and vegetables and food-borne pathogenic bacteria and putrefying bacteria including antagonistic staphylococcus aureus, salmonella paratyphi A, vibrio parahaemolyticus, yeast and the like; moreover, the bacillus amyloliquefaciens is broad in spectrum and antibacterial and great in potential in developing novel, efficient and natural biological control and biological preservative and fresh-keeping preparation.

The invention relates to a biological agent against crucifer club root and application thereof, belonging to the technical field of bio-pesticide. The strain for production is Bacillus subtilis XF-1, whose preserving number is CGMCC NO.2357. The stain has the characteristics as follows: (1) the primary colony on the LB culture substrate is white and round having a wet surface; the later colony is light yellow having uneven edge with dry and crimple surface; observed from the microscope, the strain is short-bar shaped and movable with spore, peritricha and dimension of 0.7 - 0.8 * 2.0-2.4 mum; (2) the strain is Gram-positive and aerobic and makes use of glycogen, sugar, citrate, gelatin hydrolysate, starch and casein, but does not make use of cellulose, tyrosine and catalase positive; (3) the stain has the function of sterilization, disease prevention, and yield improvement. The embodiment of the invention is as follows: using the test tube of Bacillus subtilis XF-1 stain, shake cultivation, and culture solution for fermentation to prepare biological agent, then applying the biological agent to the rhizosphere soil of crucifer crops, thereby having good effect in preventing and treating, and simple production.

The present invention relates to bacillus amyloliquefaciens and a preparation method of a microbial inoculum of the bacillus amyloliquefaciens, and belongs to the field of bio-pesticides and plant protection. The bacillus amyloliquefaciens HN-11 is isolated from azadirachta indica tree rhizosphere soil, azadirachta indica residue is used as a medium raw material and a microbial inoculum adsorption carrier for secondary solid state fermentation composting to prepare the microbial inoculums, the microbial inoculums comprises an arbitrary content of the bacillus amyloliquefaciens, 0 to 30% of the azadirachta indica residue, 4 to 12% of the soybean powder, 5 to 25% of wheat bran, 30-50% of peat, 1-5% of KCl, 0.5 to 2% of urea and 1 to 8% of a surfactant. Tests show that the microbial inoculum can overcome the problem of seedling injury caused by direct use of the azadirachta indica residue, can expand the control spectrum, has obvious integral control effect on banana wilt and Radopholus similes Thorne, can also promote plant growth, and the field control effect on the banana wilt can reach 96.2%. The microbial inoculum has great significance to the promotion of integral pest control and agricultural and sideline product recycling, and has broad application prospects in the production practice.

The invention provides a soil improvement formula. The soil improvement formula comprises a soil conditioner, a microbial agent and biostimulant, the soil conditioner is prepared from kitchen organicwaste, and the microbial agent is prepared from 5 wt%-10 wt% of actinomycetes, 10 wt%-20 wt% of nitrogen-fixing bacteria, 5 wt%-10 wt% of paecilomyces lilacinus, 10 wt%-25 wt% of trichoderma viride, 5wt%-20 wt% of lactic acid bacteria and 20 wt%-35 wt% of bacillus subtilis. The biostimulant comprises seaweed extract and / or crustacean extract, wherein the mass ratio of the soil conditioner to themicrobial agent to the biostimulant is (300-800): (150-200): (60-120). According to the soil improvement formula, the secondary metabolism level of apples can be increased, and the external light color and flavor of fruits are improved. The invention further provides an application of the soil improvement formula in apple planting.

The present invention relates to a purple sweet potato feed of microbial fermentation, which is made of the fermentation from the microbial mother liquor together with the inoculated purple sweet potato mixture; wherein the strains for the microbial mother liquor is 400 to 500 ml of starch solution bacillus, 100 to 200 ml of condensation bacillus, 300 to 400 ml of lactobacillus acidophilus, 10 to90 ml of Candida utilis; the purple sweet potato comprises 14 to 34 percent of purple sweet potato, 10.7 to 18.7 percent of chili powder, 10 to 18 percent of chrysanthemum pollen, 0.3 to 0.6 percentof sodium hydroxide, 0.7 to 1.0 percent of ammonium sulfate, 40 to 52 percent of water. Other plant wastes from which the pigment has been extracted are added into the remaining purple sweet potato from which the pigment also has been extracted, and through the microbial fermentation which is transformed into the feeds for pigs, chickens and geese, which improves the palatability of the feed, increases feed enzyme components and decreases the cost, which is of reasonable design, simple operation, convenient production, and is suitable for the broad farmers and breeding plants.

The invention discloses a fungicide containing bacillusamyloliquefaciens and application thereof, belonging to the field of microorganisms. The water content of the fungicide is not higher than 35%. The fungicide is prepared in a way of absorbing a bacillus fermentation liquid by using a carrier. The fungicide disclosed by the invention has the advantages of organic fertilizers and microbial fertilizers and has the functions of improving the utilization ratio of the fertilizer, increasing soil organic matters, promoting the breeding of rhizosphere microorganisms of crops and improving the physicochemical property and biological activity of soil. The fungicide provides comprehensive and balanced nutrition for growth of crops and can prevent and reduce soil-borne diseases, so that the yield and quality of crops are remarkably enhanced.

The invention belongs to the field of agricultural biological control, and particularly discloses a Bacillus amyloliquefaciens strain HMB27684 for control of crop greensickness, and the strain has a preservation number of CGMCC No.11456. The present invention also discloses a microbial agent prepared from the strain, and application thereof to control of crop greensickness. The invention provides an efficient microbe for the control of greensickness of cotton, eggplant and other crops, has average control effect of more than 70% on cotton and eggplant, and has strong pertinency; the microbial agent of the invention is safe to human and livestock, and has little environmental pollution; in addition, the strain has long effective period on the control of greensickness on cotton and eggplant, and does not produce drug resistance. The invention has the advantages of simple preparation method, low cost and easy usage.

The invention belongs to the technical field of soil improvement, and discloses a tea acidified soil remediation bio-organic fertilizer and a preparation method thereof. The tea acidified soil remediation bio-organic fertilizer is prepared from the following components in parts by mass: 20 to 25 parts of biochar, 10 to 15 parts of plant ash, 10 to 15 parts of river sludge, 15 to 20 parts of earthworm powder, 8 to 10 parts of animal waste, 5 to 10 parts of calcium magnesium phosphate fertilizer, 5 to 8 parts of silkworm excrement, 5 to 8 parts of zinc oxide, 5 to 8 parts of montmorillonite, 1 to 3 parts of clay mineral, 1 to 3 parts of rice husk silicon alkali and 1 to 3 parts of bacillus amyloliquefaciens. The organic fertilizer disclosed by the invention can effectively improve the pH of soil and improve the yield and quality of tea leaves; meanwhile, cost is low, raw materials are easy to obtain, the manufacturing method is simple and convenient, and the fertilizer contains rich medium-trace elements and has a soil disinfection function. The soil conditioner can improve the physical and chemical properties of soil, mainly can adjust the pH value of the soil, prevent soil hardening, improve the granular structure of the soil, loosen the soil and increase air and moisture.

The invention discloses bacillus amyloliquefaciens. The strain is preserved in the China General Microbiological Culture Collection Center on July 14th, 2015, and the preservation number is CGMCC No.11067. The invention further discloses a preparation method of the bacillus amyloliquefaciens. The invention further discloses protease produced by the bacillus amyloliquefaciens and further discloses an application of the bacillus amyloliquefaciens in tanning depilation. The protease produced by the bacillus amyloliquefaciens has good depilation activity in sheepskin, pigskin and cow leather which are frequently used in tanning, wherein the depilation effects on the cow leather and the sheepskin are the best, naked leather which is subjected to the depilation is soft, a softening effect is achieved, the grain surface is smooth, and pores are clear.

The invention discloses a pig-origin bacillus amyloliquefaciens spray drying technology, and relates to the field of pig-origin bacillus amyloliquefaciens preparation technologies. The technology comprises the steps that firstly, pig-origin bacillus amyloliquefaciens fermented liquid is prepared; secondly, a protective agent with the concentration of 20% (W / V) is added into the pig-origin bacillus amyloliquefaciens fermented liquid obtained in the first step; thirdly, spray drying is carried out by a spray drying machine, wherein the feeding speed is 1200 mL / h, the inlet temperature is 150 DEG C, and the outlet temperature is 90 DEG C. The pig-origin bacillus amyloliquefaciens spray drying technology is used for pig-origin bacillus amyloliquefaciens spray drying.

The invention provides a hermetia illucens compound amino acid fertilizer and a preparation method thereof.The preparation method comprises the steps that 1, smashing and drying dried hermetia illucens bodies, and stirring and mixing obtained powder and dried grape skin powder residues under hot air according to the mass ratio of (50-80): 1; (2) inoculating bacillus amyloliquefaciens Bt521 into aseed culture medium, and carrying out fermentation, so as to obtain a seed solution; (3) adding 50-60% of an obtained composite hermetia illucens powder into a fermentation medium; (4) inoculating 1-3% of the seed solution to the fermentation medium, and carrying out oscillation fermentation at 26-30 DEG C and 150-250 rpm for 72-78 h to obtain the hermetia illucens compound amino acid fertilizer;the prepared compound amino acid fertilizer has the advantages that the raw materials are rich in protein, the degradation rate of hermetia illucens is high, the fermentation period is short, the varieties of amino acids are rich, and the content of the amino acids can reach 0.32 mg / mL.

The invention discloses a seedling raising substrate for cultivating salt resistant plant seedlings, and a preparation method and application of the seedling raising substrate for cultivating salt resistant plant seedlings. The seedling raising substrate for cultivating salt resistant plant seedlings is prepared from mushroom residues, livestock and poultry excrement and urine, vinegar residues, gypsum residues, vermiculite, functional microorganisms and plant nutrient substances. The preparation method comprises the steps of preparing thoroughly decomposed mushroom residues through stacking fermentation of the mushroom residues, the livestock and poultry excrement and urine and the vinegar residues, preparing plant nutrition element enveloping bodies through entrapping potassium nitrate and magnesium sulphate with urea-formaldehyde resins, preparing microorganism enveloping bodies through entrapping functional microorganisms of trichoderma harzinum T83 and bacillus amyloliquefaciens IAE with polyglutamic acid, and preparing the substrate through solid fermentation and compounding of raw materials. The prepared substrate has stable high-cation environment, resistance of seedlings to hyperosmosis is continuously trained, and seedling raising substrate products suitable for heavy-salt soil regions are developed. The salinity of transplanted soil, which can be borne by plant seedlings cultivated by the products, is increased by 50% to 200%.

The invention discloses a process for producing butyric acid through co-culture and fermentation of bacillus amyloliquefaciens and clostridium butyricum. The process comprises the following steps: a fermentation process is divided into three stages including primary seed culture, secondary seed culture and fermentation culture; bacillus amyloliquefaciens and clostridium butyricum are subjected to shake-flask seed culture, inoculated into a primary seed culture medium for primary seed culture, then inoculated into a secondary seed culture medium for secondary seed culture, and finally inoculated into a fermentation tank culture medium for fermentation culture; the fermentation culture is divided into two stages; in the first stage, when the culture temperature is 30-35 DEG C and the fermentation pH is reduced to 4.5-5.0, reducing sugar is controlled in 10-20 hours; the temperature is increased to 35-37 DEG C for secondary culture; and the pH value is controlled to be 6.0. According to the invention, the starch is used for producing small molecular sugar to replace glucose in a fermentation culture medium; therefore, the cost of glucose in raw materials is reduced; the production efficiency of butyric acid is improved; the production cost is reduced; and the fermentation efficiency is improved.

The invention discloses an ecological base stock for field planting engineering of a mangrove forest, a preparation method for the ecological base stock and application of the ecological base stock. The ecological base stock for field planting engineering of the mangrove forest is prepared from a porous material, peat, a functional microbe and decomposed manure of livestock and poultry, wherein aratio (in parts by mass) of the porous material to the decomposed manure of livestock and poultry to the peat is (1 to 5): (2 to 3): (5 to 10); and the addition level of the functional microbe accounts for 1.5%-3.5% the total mass of the ecological base stock. The functional microbe is bacillus amyloliquefaciens IAE. The porous material comprises ceramsite, biochar large granules and a macro-porosity lightweight substance; the peat comprises pearlite and coco coir; and the decomposed manure comprises fermented chicken manure and cattle manure. The ecological base stock is laid in holes of a tidal flat for transplanting young mangrove plants. According to the ecological base stock, pores of soil / deposit are increased, water exchange capacity of the deposit is improved, the oxygen content ofroot systems of the young mangrove plants is increased, and the survival rate and growth speed of the young mangrove plants are increased.

The invention relates to a multifunctional compound fermentation inoculant and a preparation method and application thereof. The multifunctional compound fermentation inoculant comprises the following components in parts by weight: 5 to 15 parts of bacillus megatherium, 3 to 12 parts of bacillus licheniformis, 4 to 15 parts of bacillus amyloliquefaciens, 5 to 17 parts of bacillus pumilus, 8 to 20 parts of pseudomonas putida, 5 to 15 parts of bacillus mucilaginosus, 1 to 5 parts of aspergillus niger, 3 to 10 parts of aspergillus oryzae, 1 to 8 parts of paecilomyces lilacinus, 3 to 12 parts of trichoderma, 3 to 10 parts of streptomyces jingyangensis, 2 to 8 parts of candida utilis and 3 to 12 parts of lactic acid bacteria. Compared with the prior art, the fermentation inoculant disclosed by the invention can be mutually matched in each stage of organic waste fermentation; the fermentation inoculant is rapid to respond, has high-temperature tolerance, efficiently kills pathogenic microorganisms, can be used for preparing a bio-organic fertilizer prepared by fermenting traditional raw materials such as livestock and poultry manure and straw, can also be used for fermenting kitchen waste, municipal sludge and the like to form an organic fertilizer raw material, and has a wide application prospect.

The invention provides a microbial soil improvement microbial agent for preventing and treating tobacco epidemic diseases. The microbial soil improvement microbial agent comprises bacillus amyloliquefaciens cd5bc rape straw fermentation products and streptomyces oxysporus WS-29246. The microbial soil improvement microbial agent comprises bacillus amyloliquefaciens cd5bc rape straw fermentation products and streptomyces oxysporus WS-29246, the preservation number of the bacillus amyloliquefaciens cd5bc is CCTCC (China Center For Type Culture Collection) NO: M 2016232; the preservation number of the streptomyces oxysporus WS-29246 is CCTCC (China Center for Type Culture Collection) NO: M 2012413; the bacillus amyloliquefaciens cd5bc rape straw fermentation product is prepared by taking rape straw as a fermentation substrate and inoculating bacillus amyloliquefaciens cd5bc as zymophyte for fermentation. The control effects of the composition on the tobacco black shank and the tobacco root black rot can reach 85% or above; cadmium accumulation in tobacco can be effectively reduced, lysine in the tobacco can be effectively reduced, the quality of the tobacco can be improved, harm to a human body is reduced, and the method has a good application prospect.

The invention belongs to the field of gene and biological fermentation, and discloses the application of ornithine cyclohexanase in improving the yield of ibuprofen A produced by bacillus amylolyticus. A plasmid pHY300PLK is used as a basis, by constructing an over-expression vector pHY-ocd of ornithine cyclohexanase Ocd from corynebacterium glutamicum, expression of a gene ocd is promoted in thebacillus amylolyticus LX-12 successfully, and bacillus amylolyticus engineered strains LX-12 / pHY-ocd are obtained. Compared with control strains LX-12 / pHY300, the yield of IturinA of the engineered strains LX-12 / pHY-ocd is increased by at least 37%.

The invention provides a method for preparing a pesticide by fermenting polygonum orientale. The method is technologically characterized in that the polygonum orientale is used as a main raw material and is assisted by domestic garbage, starchy materials and water, commercially available bacillus pumilus, bacillus thuringiensis and starch hydrolyzing bacillus H47 are used as fermenting strains, pH is kept to be 5-8 according to the ratio of the polygonum orientale to the domestic garbage to the starchy materials to the purified water of (2-5): (1-2): 0.01: (3-7), the 2-5% strain inoculation quantity of a system, the fermentation temperature of 25-35 DEG C and the rotating speed is 100-200 rpm, and the fermentation time is 6-9 days. Complete fermentation is achieved, so that various enzymes, vitamins, amino acids and trace elements are fully released, and the efficacy and function of the pesticide are improved. In addition, the physiologically active substances of the plant pesticide are also not affected.

The invention relates to bacillus amyloliquefaciens KY09 and application thereof in deodorization. The classification name of the bacterial strain is Bacillus amyloliquefaciens KY09, the bacterial strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO:M 2020153. The strain can remove ammonia nitrogen and hydrogen sulfide at the same time, can effectively degrade odor generated by kitchen garbage fermentation, reduces odor pollution, and has the advantages of high efficiency and no need of additional energy consumption. When the strain is used for treating the odor of the kitchen garbage, the energy consumption in the resource utilization process of the kitchen garbage can be remarkably reduced, energy conservation and emission reductionare achieved, and more economic values are brought to resource utilization of the kitchen garbage.

The invention relates to the field of biological control and bacterial manure, in particular to bacillus amyloliquefaciens GLD-191, a microbial agent of the bacillus amyloliquefaciens GLD-191 and a preparation method and application of the bacillus amyloliquefaciens GLD-191. The bacillus amyloliquefaciens GLD-191 strain is deposited in the China General Microbiological Culture Collection Center onDecember 19, 2018, and the preservation number is CGMCC No.17011. The invention further provides the microbial agent prepared from the bacillus amyloliquefaciens GLD-191, a preparation method of themicrobial agent, and application of the bacillus amyloliquefaciens GLD-191 and the microbial agent thereof in the prevention and treatment of plant diseases. The bacillus amyloliquefaciens GLD-191 andan isatis root slag solid medium are subjected to secondary fermentation to prepare the novel microbial agent, the utilization rate of nutrients in isatis root slag is improved, and the bacillus amylolyticus GLD-191 can produce antibacterial substances with antimicrobial activity against plant pathogenic fungi, the population of beneficial microorganisms is increased, and the spectrum of diseasecontrol is expanded.

The invention discloses a fermentation broth processing method capable of improving final concentration of pullulanase, and belongs to the technical field of enzyme engineering and fermentation engineering. According to the fermentation broth processing method capable of improving final concentration of pullulanase, thermal insulation is carried out after mixing of a treatment solution C with a pullulanase fermentation liquid supernatant; and then, ultra-filtration concentration is carried out so as to obtain pullulanase fermentation broth with high enzyme activity. Being applied in post-extraction stage of pullulanase prepared by performing fermentation with recombinant bacillus amylolyticus BF7658 / pUC980-BdP, the fermentation broth processing method is capable of improving ultrafiltration performance of the pullulanase fermentation broth. Being subjected to ultra-filtration concentration, the enzyme activity of the concentrate prepared from the fermentation broth liquid supernatant by the fermentation broth processing method disclosed by the invention is higher than 1.75 times of the enzyme activities of concentrates prepared from fermentation broths which are not yet treated with the treatment solution C after the same ultra-filtration concentration process.

The invention discloses soy sauce flavor type liquor with steamed fragrant grains and application thereof. A method includes pretreating raw materials, namely weighting crushed wheat, wheat bran and rice husk in proportion of 1: 4 to 6: 2 to 3 by mass; mixing evenly, adding 37% to 40% of the raw materials of water with raw material and mixing evenly, and steaming the material at ordinary pressure for 1 to 2 hours; mixing liquids of Bacilluslicheniformis, Bacillussubtilis and Bacillusamyloliquefaciens in a proportion of 1.5 to 2.5 :0.8 to 1.2: 0.8 to 1.2 by weight; taking out the steamed materials and scattering the materials to cool at the temperature lower than 35 DEG C, adding 4% to 8% of the raw materials of microorganisms by mass to produced soy sauce flavor, and mixing evenly; placing on bed, keeping the room temperature ranging from 34 to 37 DEG C, when the temperature of the materials is up to 55 DEG C, spreading and cultivating for 36 hours; cultivating bacteria and generating flavor of the grain material for 2 to 3 days until the temperature of the grain material is close to the room temperature, and taking out the grains and storing for 5 to 10 days to obtain the finished product. By the aid of the soy sauce flavor type liquor with the steamed fragrant grains, utilizing rate and product quality of raw materials of soy sauce flavor type liquor are improved.

The invention relates to a combined fertilizer and a fertilizing method thereof. The combined fertilizer comprises a soil conditioning fertilizer and a medium trace element fertilizer, and the soil conditioning fertilizer comprises the following components in parts by mass: 20-30 parts of shells, 10-15 parts of a first growth conditioner, 49-66 parts of a calcium magnesium phosphate fertilizer, 2-3 parts of bacillus megatherium and 2-3 parts of bacillus amyloliquefaciens; the medium trace element fertilizer comprises, by mass, 39.2-56.5 parts of liquid base fertilizer, 20-25 parts of a secondgrowth conditioner, 5-8 parts of a phosphate fertilizer, 0.2-0.3 parts of a boron-containing compound, 4-6 parts of a potassium fertilizer, 4-7 parts of a nitrogen fertilizer, 0.3-0.5 parts of a selenium-containing compound, 6-8 parts of a zinc-containing compound and 4-6 parts of an iron-containing compound; wherein the first growth conditioner and the second growth conditioner are respectively selected from at least one of fulvic acid, humic acid, alkali lignin and amino acid powder. When the combined fertilizer is applied to crops, a crop product which is rich in various nutrient elements and high in nutrient element content can be harversted.

The invention discloses bacillus amyloliquefaciens for inhibiting soy sauce from forming albuginea and application of the bacillus amyloliquefaciens, and belongs to the technical field of microorganisms. According to the invention, soy sauce mash microorganisms are separated and purified to obtain the strain bacillus amyloliquefaciens JDF-1 capable of inhibiting pichia pastoris or albuginea produced by pichia pastoris and inhibiting or eliminating formation of albuginea in soy sauce; and in a simulated soy sauce fermentation system, the bacillus amyloliquefaciens JDF-1 is added, so that generation of albuginea in the system can be inhibited. The bacillus amyloliquefaciens JDF-1 obtained by screening is suitable for a soy sauce fermentation production environment, and albuginea in soy sauce can be well generated by adjusting the addition amount of the strain, so that the bacillus amyloliquefaciens JDF-1 has important significance in reducing the generation of the albuginea and improving the quality of the soy sauce in the soy sauce fermentation production.

The invention belongs to the technical field of cultivation. The invention discloses a livestock and poultry manure composite sulfuric acid type furfural residue organic fertilizer and a preparation method thereof. The livestock and poultry manure composite sulfuric acid type furfural residue organic fertilizer is prepared from livestock and poultry manure, furfural residues, 2-5 parts of lactobacillus acidophilus liquid, 2-4 parts of bacillus subtilis, 0-3 parts of bacillus amyloliquefaciens, 2-5 parts of bacillus megatherium, 1-3 parts of bacillus cereus and 0-2 parts of a mould liquid. Theorganic fertilizer disclosed by the invention can improve soil and improve organic matters; one-step deodorization is achieved, the efficiency reaches 85% or above, and the air quality is improved; the temperature rises quickly and reaches 70 DEG C or above within 2-3 days, moisture in livestock and poultry manure and furfural residues can be removed quickly, fermentation is conducted for about 15days, and the moisture content of the mixed material reaches about 35%; meanwhile, environmental pollution sources of the two wastes are eliminated; the escherichia coli killing rate of the fermentedproduct exceeds 99.99%, and heavy metals are solidified.