44 results about "Bacillus amylolyticus" patented technology

The invention discloses a bacillus subtilis strain and application thereof, the bacillus subtilis strain is bacillus subtilis X-NY1 and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 20242038. Bacillus subtilis X-NY1, bacillus velezensis and bacillus amyloliquefaciens are compounded into a microbial agent, a film-forming agent, a dispersing agent, a coloring agent, a stabilizing agent, a preservative, a conditioning agent and a nutritional agent are further added to prepare the compound microbial seed coating agent, the effective viable count of the microbial agent is 108-1011, and the quality guarantee period is longer than 6 months. The compound microbial seed coating agent can improve the plant height, root length, fresh weight and the like in the growth process of pennisetum alopecuroides, can effectively improve the stress resistance of the pennisetum alopecuroides under the salinization condition, and is small in addition amount, low in cost, safe, reliable, obvious in growth promoting effect and suitable for application and popularization.

The invention discloses bacillus amyloliquefaciens which is derived from yeast for making hard liquor and has multiple tolerance and hexanoic acid production characteristics as well as a separation method and application of the bacillus amyloliquefaciens. The strain is separated from yeast for making hard liquor, can produce hexanoic acid, has the characteristics of high temperature resistance, acid resistance, ethanol resistance and the like, is identified as bacillus amyloliquefaciens DJ001, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has the preservation number of CGMCC No.33299. The invention further discloses a method for preparing the caproic acid-resistant yeast for making hard liquor for making hard liquor for making hard liquor for making hard liquor for making hard liquor for making hard liquor for making hard liquor for making hard liquor for making hard liquor for making hard liquor for making hard liquor. When the strain is added into yeast for making hard liquor, the hexanoic acid production capacity and saccharification capacity of the yeast for making hard liquor can be improved, and when the strain is added into fermented grains, the yield of white spirit and the content of ethyl hexanoate can be improved. The method has important values in the aspects of improving the yield and quality of the white spirit, saving the cost, improving the efficiency and the like, and can increase enterprise and social benefits.

The invention provides a production method of a growth-promoting granular bio-organic fertilizer, and relates to the technical field of organic fertilizers. According to the invention, a mixture of bacillus amyloliquefaciens and bagasse is added into a decomposed organic fertilizer for secondary fermentation treatment, then the material after secondary fermentation is subjected to low-temperature drying and then is subjected to granulation treatment, and a potassium fulvic acid aqueous solution is added in the granulation process to be used as a binder, so that the fertilizer efficiency of the biological organic fertilizer is improved; meanwhile, the use amount of a traditional binder is reduced.

The invention belongs to the field of microorganisms and fermentation, and relates to a biological preparation, a preparation method thereof and application of the biological preparation in preparation of products for inhibiting escherichia coli. And the biological agent is a co-fermentation product of fructus forsythiae and bacillus amyloliquefaciens (Bacillus amyloliquefaciens). The biological preparation provided by the invention can obviously improve the secretion of IFN-alpha in a mouse body, reduce the production of IL-1beta, IL-6 and TNF-alpha, increase the proportion of CD3 + CD4 + and CD3 + CD8 + T cells, and improve the expression of ZO-1 and Occludin in duodenum and jejunum. Compared with single use of fructus forsythiae and bacillus amyloliquefaciens, the biological preparation shows a stronger synergistic effect, can effectively prevent and inhibit escherichia coli infection, and is not easy to generate drug resistance. The preparation method is simple, raw materials are easy to obtain, and the feed additive is suitable for developing antibacterial drugs and feed additives.

The invention belongs to the technical field of greenhouse vegetable salinization soil improvement, and particularly relates to a preparation method of a compound microbial agent for greenhouse vegetable salinization soil improvement. Comprising the following steps: S1, preparing a fermentation substrate: mixing bran, molasses, ammonium sulfate, rice hull powder and water according to a mass ratio, and sterilizing at 121 DEG C for 30 minutes for later use; s2, preparing biochar: preparing modified biochar with MgO loading capacity of 4-6% and magnesium-loaded biochar with MgO loading capacity of 5-7%; s3, preparing a compound bacterium suspension: respectively preparing bacillus amyloliquefaciens, bacillus mucilaginosus, trichoderma viride, rhizobium and saccharomyces cerevisiae into 5 * 10 < 10 > CFU / mL bacterium suspensions, and mixing the bacterium suspensions according to a volume ratio to obtain the compound bacterium suspension. Through cooperation of multiple strains and charcoal-loaded magnesium, the salinization index of the facility vegetable soil can be remarkably reduced, the soil fertility is comprehensively improved, the micro-ecology of the soil is restored, and the growth and quality of vegetables are promoted.

The invention discloses a quality-improving and yield-increasing method for salvia miltiorrhiza based on straw source smoke water and bacillus amyloliquefaciens, and relates to the technical field of planting methods. According to the method, the tobacco water and the bacillus amyloliquefaciens are compounded to obtain a mixed solution, and the mixed solution is adopted to perform root soaking and foliage spraying on the salvia miltiorrhiza, so that the yield and the quality of the salvia miltiorrhiza are improved, and particularly, the tobacco water and the bacillus amyloliquefaciens are combined for use; the single plant accumulation amounts of the tanshinone I, the tanshinone IIA, the cryptotanshinone and the salvianolic acid B are increased by 20.33%, 12.92%, 26.53% and 102.01% compared with those of a group without treatment, and the bacillus amyloliquefaciens has a synergistic effect on increasing the content of medicinal components in the radix salviae miltiorrhizae. In addition, by means of the method, the yield and agronomic characters of the salvia miltiorrhiza can be improved, specifically, the weight, SPAD value, net photosynthetic rate, stomatal conductance and nitrogen phosphorus and potassium accumulation amount of the salvia miltiorrhiza roots are improved.

The invention discloses a liver-benefiting fermented Chinese herbal medicine composition and a preparation method thereof, which belongs to the technical field of Chinese medicine fermentation, and comprises a Chinese herbal medicine composition and a probiotic composition, wherein the mass ratio of the Chinese herbal medicine composition to the probiotic composition is (1500-2500): (10-30); the Chinese herbal medicine composition is composed of the following components: Artemisia capillaris, Astragalus, green tea extract, Plantago, mint, Coix seeds, safflower, borneol, wolfberry, Lycium barbarum, Lysimachia chinensis, honeysuckle and pumpkin; the probiotic composition is composed of the following components: Bacillus amyloliquefaciens, Bacillus subtilis, Lactobacillus plantarum, Bifidobacterium, pectinase and β-glucanase. The problem of low bioavailability and unstable efficacy of traditional Chinese medicine preparations is solved. It has the advantage of promoting the release and absorption of effective ingredients.

The invention discloses a bacillus amyloliquefaciens B2 suspending agent and a preparation method and application thereof, the bacillus amyloliquefaciens is named as B2, the preservation number is CGMCC No.30634, and the bacillus amyloliquefaciens B2 suspending agent prepared from the bacillus amyloliquefaciens B2 suspending agent can be used for preventing and treating potato scab. By optimizing the formula of the fermentation culture medium and various process parameter conditions, B2 fermentation liquor of which the concentration is greater than or equal to 8 billion / mL is obtained; the bacillus amyloliquefaciens B2 suspending agent with the concentration of 2 billion / mL is researched and developed through auxiliary agent screening and compatibility testing of auxiliary agents. Field experiments show that the 2 billion / mL bacillus amyloliquefaciens B2 suspending agent has a good biocontrol effect on potato scab, and the yield of potatoes and the income of farmers are obviously increased.

The invention belongs to the technical field of microbial fermentation, and particularly provides a fermentation production method of mannase, which comprises the following steps: simultaneously inoculating saccharomyces cerevisiae and bacillus amyloliquefaciens into a fermentation base material for fermentation to obtain the mannase. In the fermentation process of the saccharomyces cerevisiae, a large amount of reducing sugar can be used for metabolism to generate ethanol, and the ethanol is gradually accumulated in a fermentation system along with the growth metabolism of the saccharomyces cerevisiae, so that more and more bacillus amyloliquefaciens form spores. When the saccharomyces cerevisiae is large in volume, the growth of the saccharomyces cerevisiae is slowed down by feeding after starvation, and meanwhile, the temperature and the pH are manually adjusted to germinate the spores. As the temperature is increased, ethanol volatilization is accelerated, decline of the saccharomyces cerevisiae is accelerated, cell wall breaking metabolites are released from cells, nutrient substances in a fermentation system are rich, spores start to germinate, and bacillus amyloliquefaciens can secrete mannase to decompose the cell walls of the saccharomyces cerevisiae.

The invention belongs to the field of tea leaf microbial fermentation, and particularly relates to a tea leaf fermentation preparation method which comprises the following steps: primary treatment, fixation, micro fermentation, first heating and then cold treatment, micro heaping for yellowing, rolling and drying. According to the preparation method disclosed by the invention, in the fermentation preparation process of the wild tea, the formula of the fermentation liquor is optimized, and the bacillus amyloliquefaciens, the lactic acid bacteria and the saccharomycetes are cooperatively fermented, so that tea soup is long in aftertaste and rich in mouth feel, and the tea soup still has certain freshness at the third brewing time. Through cooperation of slight fermentation and slight heaping for yellowing, the bitter taste of the first brewing tea soup of the wild tea can be obviously reduced, and the debitterizing effect is obvious. The tea taste can be obviously prolonged by matching heat treatment with cold treatment in combination with micro fermentation and micro heaping for yellowing, and tea soup still has a certain degree of aftertaste after sixth brewing and is not boring. The toughness of the tea leaves can be remarkably improved by adopting first heating and then cold treatment, especially the defects caused by heaping for yellowing can be improved, and the tea leaves are not rotten after being boiled for a long time and are more resistant to brewing.

The invention belongs to the technical field of biological bacterial fertilizer preparation, and relates to a composite wormcast biological bacterial fertilizer and a preparation and use method thereof. Mixing and pre-fermenting the modified cow dung and the modified corn straw; performing gradient inoculation on adult and young earthworms for biotransformation to obtain composite wormcast; carrying out enzymolysis on an organic nitrogen source, and compounding with humic acid to prepare a stable nitrogen source solution; and finally, mixing the composite wormcast, a nitrogen source liquid and a specific bacillus amyloliquefaciens inoculant in proportion, and carrying out stepped temperature-controlled fermentation to obtain a finished product. According to the method, through raw material biological modification, earthworm gradient conversion, nutrient enzymolysis compounding and functional bacterium directional fermentation multi-stage cooperation, the survival rate and activity of functional bacteria in the product and the soil colonization capacity are remarkably improved, nutrient slow release and supply and demand matching are achieved, and the functions of nutrient supply, growth promotion and disease resistance and soil improvement are integrated.

The application discloses a multi-strain combined solid-state fermentation rapeseed meal probiotic agent and a fermentation method thereof, adopts Bacillus amyloliquefaciens, Saccharomyces cerevisiae and Bacillus subtilis to combine solid-state fermentation rapeseed meal to reduce the content of anti-nutritional factors such as glucosinolates, and comprises the following steps: pretreatment and sterilization of rapeseed meal raw materials, preparation of fermentation seed liquid, solid-state fermentation of probiotics, and preparation of fermented rapeseed meal products. The application firstly uses Bacillus amyloliquefaciens to solid-state ferment rapeseed meal, and combines with Saccharomyces cerevisiae and Bacillus subtilis to add into rapeseed meal to carry out combined fermentation; compared with other technical solutions, the application can greatly reduce the content of anti-nutritional factor glucosinolate in rapeseed meal, and can reduce the content of anti-nutritional factors tannin, phytic acid and crude fiber in rapeseed meal and improve the content of peptides in the rapeseed meal product, various bioactive substances can be produced in the probiotic fermentation process, and the method has the advantages of low cost, simple operation, short time consumption and high efficiency.

The invention discloses a method for reducing emission of nitrous oxide (N2O) in farmland soil based on synergistic fertilization of bacillus amyloliquefaciens EBL11, and belongs to the technical field of agricultural environment treatment. Aiming at the problems of high cost, high environmental risk, poor stability and the like in the existing N2O emission reduction technology, the bacillus amyloliquefaciens EBL11 is cooperatively applied with a fertilizer in a solid microbial inoculum form, so that the N2O emission of the farmland is remarkably reduced. The method is characterized by comprising the following steps: (1) synergistically applying a compound fertilizer and a fungicide; (2) synergistically applying an organic fertilizer and a fungicide; and (3) mixing the microbial inoculum and the fertilizer, uniformly spreading the mixture to the soil, and combining intermittent irrigation and repeated quadrat monitoring management. Experiments show that the method can reduce the N2O emission rate of the paddy field by 131.2%-215.4% within one week, soil is converted into an absorption state from emission, and meanwhile, the biomass of crops such as oilseed rape is increased. The method has the characteristics of high efficiency, emission reduction, low cost and environmental friendliness, and is suitable for large-scale popularization in farmlands such as paddy fields and rape fields.

The application provides a method for processing cigar tobacco leaves by using bacillus fermentation, a bacterial agent for fermenting and processing cigar tobacco leaves and application thereof, and belongs to the technical field of tobacco fermentation. The bacillus is inoculated and cultured to obtain a seed liquid, the seed liquid is inoculated into a culture medium containing wine lees at an inoculation amount of 1-5% (v / v), and the culture is cultured at 30-40 DEG C for 12-14h to obtain a bacillus liquid; the bacillus liquid is sprayed on the cigar tobacco leaves, and the cigar tobacco leaves are subjected to temperature combination fermentation and then balanced in moisture. The bacillus is bacillus amyloliquefaciens SS0813, bacillus velezensis MZ1030 or bacillus aryabhattai GL0525. The strains are combined with temperature combination fermentation to process the cigar tobacco leaves, the content of various aroma components in the cigar tobacco leaves can be stably increased, the stimulating aroma components such as nicotine are obviously reduced, and the cigar tobacco is more mellow when smoked.

The invention belongs to the field of plant growth regulators, and particularly relates to a plant growth regulator containing camellia oleifera residues and a preparation method of the plant growth regulator. According to the plant growth regulator, waste oil tea residues, shiitake mushroom dregs, distillers' grains, bagasse and banana stems and leaves generated in the agricultural production process serve as fermentation substrates, trichoderma harzianum and saccharomycetes are added for fermentation, then livestock and poultry slaughter waste and bacillus amyloliquefaciens are sequentially added for fermentation treatment, and the plant growth regulator is obtained. Through staged fermentation culture and mutual influence and synergistic effect between different microorganisms and substrates, the finally prepared plant growth regulator can significantly improve plant growth and increase plant height and stem diameter of plants, has a significant growth promoting effect, realizes recycling of resources, meets the requirements of green and environmental protection, and is worthy of popularization and application. And a new thought is provided for sustainable circular agriculture.

The invention provides a saline-alkali soil improver and a method for improving saline-alkali soil, and belongs to the technical field of soil remediation and agricultural resource utilization. The saline-alkali soil improver is prepared from polymeric amino acid, humic acid, phosphogypsum, pyroligneous liquor, oxalic acid, a compound microbial agent and biochar. The compound microbial agent is obtained by mixing bacillus subtilis, pseudomonas, bacillus amyloliquefaciens and citrobacter Young. The invention further provides a method for improving the saline-alkali soil, the saline-alkali degree of the soil can be rapidly reduced, the granular structure is improved, the soil fertility is improved, and the yield and quality of crops in the saline-alkali soil are improved.

The invention discloses a plant stress-resistant nutrient solution mother liquor composition as well as a preparation method and application thereof. The plant stress-resistant nutrient solution mother liquor composition consists of a first stress-resistant mother liquor and a second stress-resistant mother liquor, the first stress-resistant mother liquor is formed by mixing a betaine aqueous solution and a carboxymethyl chitosan acetic acid solution; the second stress-resistant mother liquor is prepared by mixing a folium cortex eucommiae extract ethanol solution, a bacillus amyloliquefaciens fermentation supernatant and a nano-hydroxyapatite suspension according to a volume ratio of (1.5-2.5): (2.5-3.5): (0.8-1.2). The stress-resistant nutrient solution mother liquor composition is designed, multiple components including betaine, chitosan, eucommia ulmoides extract, bacillus amyloliquefaciens and nano-hydroxyapatite are synergistically compounded and applied through root irrigation, so that the stress resistance of crops is stably improved for a long time, and the defects that traditional foliage dressing is short in lasting effect, high in operation frequency and limited by the environment are effectively overcome.

The application provides application of a bacillus amyloliquefaciens in soy product processing wastewater, and belongs to the technical field of biotechnology. The bacillus amyloliquefaciens with a strain preservation number of CCTCC NO: M2021847 is used as a fermentation strain, and soy product processing wastewater is used as a main component of a culture medium for fermentation to prepare bacillus amyloliquefaciens fertilizer. The fertilizer can be used in agricultural production to obviously improve crop yield, especially the base stem diameter of crops, thereby playing a role in anti-lodging and anti-natural disasters, reducing the use amount of traditional fertilizers, reducing costs, realizing resource utilization of soy product wastewater, and reducing wastewater discharge.

The invention provides a trichoderma inoculant, a preparation method thereof and application of the trichoderma inoculant in improving yield and quality of beet, and belongs to the technical field of agricultural microbial inoculants. The trichoderma agent comprises the following components: trichoderma harzianum, trichoderma viride, purpureocillium lilacinum, bacillus amyloliquefaciens and bacillus laterosporus in a mass ratio of (3-5): (1-3): (2-4): (2-4): (1-2). According to the invention, the strains cooperate with each other to promote the growth of beet, effectively enhance the immune resistance of beet, and effectively prevent and reduce beet root rot, brown blotch and other diseases caused by continuous cropping; the photosynthesis efficiency of the beet can be enhanced, the accumulation of photosynthetic products is improved, the soil structure is improved, the number of beneficial bacteria groups in the soil is increased, and the rhizosphere environment is improved, so that the sugar content and yield of the beet are increased. By applying the microbial agent disclosed by the invention, multiple effects of increasing the yield per mu, increasing the sugar content of beet, improving soil, improving the fertilizer efficiency, resisting diseases, preventing insects and the like can be achieved, and farmers are helped to increase both production and income.

The invention discloses a bifunctional xylanase fusion protein, a preparation method thereof and application of the bifunctional xylanase fusion protein in preparation of feruloyl oligosaccharide, and belongs to the technical field of genetic engineering and enzyme engineering. The fusion protein is composed of GH10 xylanase derived from bacillus altitudinis, a connecting peptide and GH11 xylanase derived from bacillus amyloliquefaciens. According to the invention, through an elaborately designed linker, the GH10 family xylanase and the GH11 family xylanase are fused through a specific connecting peptide, so that mutual interference between structural domains can be effectively overcome, the synergistic catalysis of GH10 and GH11 is fully played, and the novel bifunctional xylanase with synergistic interaction, strong specificity, high catalytic efficiency and high thermal stability is obtained. Compared with a single enzyme or a physical compound enzyme, the compound enzyme has better hydrolysis efficiency and application performance, and can be widely applied to the fields of food, textile, paper pulp bleaching, feed industry and the like.

The present invention belongs to the technical field of microorganism and plant disease prevention and control, and specifically, relates to a composition for preventing and controlling blueberry root and stem diseases and its application, wherein the composition comprises: a) Bacillus amyloliquefaciens, b) bamboo shoot fiber powder, corn pollen polysaccharide, cattle bone meal, mineral potassium humate and polyglutamic acid, and c) biomass power plant ash; wherein the preservation number of Bacillus amyloliquefaciens is CCTCC NO: M20221206. Applying the composition during the blueberry planting process can significantly reduce the content of fungal toxins such as fusaric acid, fumonisin and trichothecene in the rhizosphere soil of blueberries, increase the content of resistance substances such as salicylic acid, jasmonic acid and phenolic substances in the root system, and reduce the fungal toxins caused by Fusarium solani ( Fusarium solani ), Fusarium equisetum ( Fusarium horsetail ), and / or, Pythium ( Pythium sterile )、Rhizoctonia solani( Rhizoctonia solani )、Fusobacterium spp. Neofusicoccus minor ) to measure the disease index of blueberry rhizome diseases caused by infection, and ultimately achieve effective prevention and control of blueberry rhizome diseases.

The present invention belongs to the field of bioengineering technology, and specifically relates to an alkaline amylase mutant and its application. The present invention mutates the amino acids M, V, Y, V, V, Y and D at positions 57, 152, 203, 223, 294, 345 and 392 in the amino acid sequence of alkaline amylase from alkaliphilic Bacillus to I, I, D, I, G, C and N, respectively, to obtain an alkaline amylase mutant, wherein the mutant has the characteristics of alkali resistance and heat resistance, and at the same time, a recombinant expression vector containing the mutant gene is transferred into Bacillus amyloliquefaciens ZD-708 (△GAΔEGL) for expression, and the mutant is expressed at 60 m 3 The average enzyme activity level in the fermentation tank reached 31243U / mL, which can further reduce the production cost of alkaline amylase and promote the widespread application of alkaline amylase in the textile and washing industries.

The invention provides a mushroom bran fermentation and amino acid extraction process, and belongs to the technical field of microorganisms. According to the invention, the mushroom bran fermentation material after fruiting is taken as a substrate, the amino acid liquid is extracted by utilizing an enzymolysis method, and the amino acid liquid can be used as an organic component of a water-soluble fertilizer in agricultural production to provide a required nutrient source for plant growth. In addition, the bacillus amyloliquefaciens JK-2 is used as a research object to carry out salt tolerance domestication, and the domesticated strain is used as a microbial component of the water-soluble fertilizer to prepare the microbial agent, so that the application effect of the novel composite water-soluble fertilizer can be improved.

This invention relates to a black rice fermentation powder, its preparation method, and its application, belonging to the field of food fermentation and processing technology. This invention provides a method for preparing black rice fermentation powder, comprising the following steps: (1) crushing black rice and sterilizing it to obtain black rice flour; (2) inoculating the black rice flour with a fermenting agent to obtain a fermentation substrate; (3) mixing the fermentation substrate with water for fermentation and freeze-drying to obtain black rice fermentation powder. This invention uses a specific combination of *Saccharomyces cerevisiae* and *Bacillus amyloliquefaciens* as a fermenting agent to ferment black rice flour. Through experiments, the optimal fermentation parameters were determined. The resulting black rice fermentation powder exhibits significantly higher levels of active ingredients and antioxidant capacity than unfermented and single-strain fermented products, fully verifying the advanced nature, synergy, and high efficiency of this application.

The invention relates to the technical field of apple cultivation, and discloses a fertilization method for improving the quality of Fuji apples and a special fertilizer system. The method comprises the following steps: (1) establishing a stepped fertilization system based on nutritional requirements in a growth period, and dividing the growth period of Fuji apples into five key stages, namely an autumn application and root raising stage, a spring germination stage, an early fruit swelling stage, a middle fruit swelling stage and a color changing stage; (2) developing a special compound fertilizer system which comprises a nitrogen-calcium synergistic fertilizer, a medium trace element slow-release fertilizer, a seaweed root-promoting barrel fertilizer and a coloring functional fertilizer; (3) adopting an organic-inorganic-biological synergistic fertilization mode, wherein an organic fertilizer contains soybean oligopeptide (the molecular weight is 800-1200Da) and a specific flora (bacillus amyloliquefaciens is greater than or equal to 0.1 billion CFU / g); and (4) a root activity regulation technology is matched, and annual activation of the root system is realized through three times of flushing application of the seaweed primary pulp. Experiments show that the single fruit weight of Fuji apples is increased by 22.3%, soluble solids are increased by 32.7%, the fruit coloring index is increased by 25.4%, and meanwhile, the use amount of the chemical fertilizer is reduced by 16.7%.

The invention relates to biological prevention and control of weeds and discloses an antagonistic microorganism combined fungicide for inhibiting growth of trifolium repens. The combined fungicide is prepared from the following active ingredients in percentage by weight: 30%-45% of bacillus amyloliquefaciens liquid, 25%-35% of pseudomonas fluorescens liquid, 20%-30% of trichoderma harzianum liquid and 5%-10% of bacillus subtilis liquid, the viable count of the bacillus amyloliquefaciens is greater than or equal to 1 * 10 < 9 > CFU / mL, the viable count of the pseudomonas fluorescens is greater than or equal to 5 * 10 < 8 > CFU / mL, the spore concentration of the trichoderma harzianum is greater than or equal to 2 * 10 < 7 > / mL, and the viable count of the bacillus subtilis is greater than or equal to 8 * 10 < 8 > CFU / mL. According to the antagonistic microorganism combined fungicide, through the synergistic antagonistic effect of bacillus amyloliquefaciens, pseudomonas fluorescens, trichoderma harzianum and bacillus subtilis, and in cooperation with a specific auxiliary agent and an adaptive use method, the antagonistic microorganism combined fungicide has the advantages that the antagonistic microorganism combined fungicide can be applied to lawn weed control, farmland intercropping and interplanting white clover prevention and control, garden green land white clover overgrowth treatment and other scenes; the growth of the trifolium repens can be effectively inhibited, and the plant height growth rate, the fresh weight and the coverage / density of the trifolium repens can be remarkably reduced.

The present invention relates to the field of polypeptides, and in particular to a marine collagenase Almanase and its application in preparing tilapia skin collagen tripeptide. The present invention screens out a S8 family serine protease Almanase from the marine genus Alteromonas sp., and its amino acid sequence is shown in SEQ ID NO: 1. The present invention also provides a Pichia yeast for producing Almanase, and Almanase with high enzyme activity can be obtained by fermentation. The marine collagenase Almanase of the present invention is compounded with commercially available alkaline protease Alcalase and Bacillus amyloliquefaciens neutral protease to produce tilapia skin collagen tripeptide. In the obtained enzymatic hydrolyzate, the proportion of oligopeptides with a molecular weight of <500Da is increased by 23%, and the content of collagen tripeptide is increased by 2.03 times.

The invention belongs to the technical field of microorganisms and baijiu brewing, and particularly relates to bacillus amyloliquefaciens capable of producing ethyl lactate and application of the bacillus amyloliquefaciens to improvement of fermented ethyl lactate of rice-flavor baijiu. The strain is separated from later-stage fermentation liquor of rice-flavor liquor, and has the characteristics of acid resistance, high-concentration ethanol resistance and high-yield ethyl lactate. According to the invention, the strain is added in the later period of fermentation of the rice-flavor liquor for enhanced fermentation, and then physical temperature control is carried out, so that the concentration of ethyl lactate can be synergistically increased, flavor substances such as total ester of the rice-flavor liquor can be remarkably increased, and meanwhile, the liquor yield is not influenced, so that the compound fragrance of rice wine is increased, and the liquor quality is improved. The method is good in stability among batches, can obtain the rice-flavor baijiu with high ethyl lactate content, and is suitable for popularization and application in baijiu brewing.

The application discloses a traditional Chinese medicine shampoo capable of inducing hair follicle regeneration and promoting hair growth and a preparation method thereof, and relates to the technical field of daily chemical products. The shampoo comprises the following components in parts by weight: Bacillus amyloliquefaciens fermentation extract 10-20 parts, Amoora roxburghii Benth extract 25-40 parts, tryptophan 1-4 parts, Myristica fragrans Houtt extract 20-35 parts, Platycladus orientalis (L.) Franco extract 20-30 parts, seaweed extract 18-26 parts, surfactant 6-15 parts, foaming agent 1-3 parts, thickening agent 0.5-1.5 parts, lubricant 0.1-0.8 parts, humectant 0.5-2.5 parts and water 800-1000 parts. The effective components of the shampoo can rapidly penetrate into the keratin layer barrier of the scalp and be absorbed by the scalp, promote the proliferation of hair follicle stem cells, prolong the growth period of the hair follicles, delay the entry of the hair follicles into the involution period, prevent the atrophy of the hair follicles and realize rapid hair growth.

The invention discloses bacillus amyloliquefaciens capable of producing heptanoic acid and nonanoic acid as well as an isolated culture method and application of the bacillus amyloliquefaciens, and belongs to the technical field of microorganisms. The bacillus amyloliquefaciens LZLJ2-11 is separated and screened from high-temperature yeast for making hard liquor, the preservation number is CCTCC NO: M 2025367, the bacillus amyloliquefaciens LZLJ2-11 can produce heptanoic acid and nonanoic acid at the same time through wheat, the performance is stable, the bacillus amyloliquefaciens LZLJ2-11 can adapt to the high-temperature environment, the high-acid environment and other environments in the liquor making process, flavor precursor substances are effectively provided when the bacillus amyloliquefaciens LZLJ2-11 is applied to liquor making, and the production efficiency is improved. The content of ethyl heptanoate and ethyl nonanoate in the fermented grains is increased, the microbial resource library of white spirit brewing is enriched, functional division of microbial communities in the white spirit brewing process is facilitated, and microbial resources are provided for research of the white spirit brewing complex microbial inoculants.