15 results about "Bacillus amylolyticus" patented technology

The invention belongs to the field of tea leaf microbial fermentation, and particularly relates to a tea leaf fermentation preparation method which comprises the following steps: primary treatment, fixation, micro fermentation, first heating and then cold treatment, micro heaping for yellowing, rolling and drying. According to the preparation method disclosed by the invention, in the fermentation preparation process of the wild tea, the formula of the fermentation liquor is optimized, and the bacillus amyloliquefaciens, the lactic acid bacteria and the saccharomycetes are cooperatively fermented, so that tea soup is long in aftertaste and rich in mouth feel, and the tea soup still has certain freshness at the third brewing time. Through cooperation of slight fermentation and slight heaping for yellowing, the bitter taste of the first brewing tea soup of the wild tea can be obviously reduced, and the debitterizing effect is obvious. The tea taste can be obviously prolonged by matching heat treatment with cold treatment in combination with micro fermentation and micro heaping for yellowing, and tea soup still has a certain degree of aftertaste after sixth brewing and is not boring. The toughness of the tea leaves can be remarkably improved by adopting first heating and then cold treatment, especially the defects caused by heaping for yellowing can be improved, and the tea leaves are not rotten after being boiled for a long time and are more resistant to brewing.

The invention belongs to the technical field of biological bacterial fertilizer preparation, and relates to a composite wormcast biological bacterial fertilizer and a preparation and use method thereof. Mixing and pre-fermenting the modified cow dung and the modified corn straw; performing gradient inoculation on adult and young earthworms for biotransformation to obtain composite wormcast; carrying out enzymolysis on an organic nitrogen source, and compounding with humic acid to prepare a stable nitrogen source solution; and finally, mixing the composite wormcast, a nitrogen source liquid and a specific bacillus amyloliquefaciens inoculant in proportion, and carrying out stepped temperature-controlled fermentation to obtain a finished product. According to the method, through raw material biological modification, earthworm gradient conversion, nutrient enzymolysis compounding and functional bacterium directional fermentation multi-stage cooperation, the survival rate and activity of functional bacteria in the product and the soil colonization capacity are remarkably improved, nutrient slow release and supply and demand matching are achieved, and the functions of nutrient supply, growth promotion and disease resistance and soil improvement are integrated.

The application discloses a multi-strain combined solid-state fermentation rapeseed meal probiotic agent and a fermentation method thereof, adopts Bacillus amyloliquefaciens, Saccharomyces cerevisiae and Bacillus subtilis to combine solid-state fermentation rapeseed meal to reduce the content of anti-nutritional factors such as glucosinolates, and comprises the following steps: pretreatment and sterilization of rapeseed meal raw materials, preparation of fermentation seed liquid, solid-state fermentation of probiotics, and preparation of fermented rapeseed meal products. The application firstly uses Bacillus amyloliquefaciens to solid-state ferment rapeseed meal, and combines with Saccharomyces cerevisiae and Bacillus subtilis to add into rapeseed meal to carry out combined fermentation; compared with other technical solutions, the application can greatly reduce the content of anti-nutritional factor glucosinolate in rapeseed meal, and can reduce the content of anti-nutritional factors tannin, phytic acid and crude fiber in rapeseed meal and improve the content of peptides in the rapeseed meal product, various bioactive substances can be produced in the probiotic fermentation process, and the method has the advantages of low cost, simple operation, short time consumption and high efficiency.

The invention discloses a method for reducing emission of nitrous oxide (N2O) in farmland soil based on synergistic fertilization of bacillus amyloliquefaciens EBL11, and belongs to the technical field of agricultural environment treatment. Aiming at the problems of high cost, high environmental risk, poor stability and the like in the existing N2O emission reduction technology, the bacillus amyloliquefaciens EBL11 is cooperatively applied with a fertilizer in a solid microbial inoculum form, so that the N2O emission of the farmland is remarkably reduced. The method is characterized by comprising the following steps: (1) synergistically applying a compound fertilizer and a fungicide; (2) synergistically applying an organic fertilizer and a fungicide; and (3) mixing the microbial inoculum and the fertilizer, uniformly spreading the mixture to the soil, and combining intermittent irrigation and repeated quadrat monitoring management. Experiments show that the method can reduce the N2O emission rate of the paddy field by 131.2%-215.4% within one week, soil is converted into an absorption state from emission, and meanwhile, the biomass of crops such as oilseed rape is increased. The method has the characteristics of high efficiency, emission reduction, low cost and environmental friendliness, and is suitable for large-scale popularization in farmlands such as paddy fields and rape fields.

The application provides a method for processing cigar tobacco leaves by using bacillus fermentation, a bacterial agent for fermenting and processing cigar tobacco leaves and application thereof, and belongs to the technical field of tobacco fermentation. The bacillus is inoculated and cultured to obtain a seed liquid, the seed liquid is inoculated into a culture medium containing wine lees at an inoculation amount of 1-5% (v / v), and the culture is cultured at 30-40 DEG C for 12-14h to obtain a bacillus liquid; the bacillus liquid is sprayed on the cigar tobacco leaves, and the cigar tobacco leaves are subjected to temperature combination fermentation and then balanced in moisture. The bacillus is bacillus amyloliquefaciens SS0813, bacillus velezensis MZ1030 or bacillus aryabhattai GL0525. The strains are combined with temperature combination fermentation to process the cigar tobacco leaves, the content of various aroma components in the cigar tobacco leaves can be stably increased, the stimulating aroma components such as nicotine are obviously reduced, and the cigar tobacco is more mellow when smoked.

The invention provides a saline-alkali soil improver and a method for improving saline-alkali soil, and belongs to the technical field of soil remediation and agricultural resource utilization. The saline-alkali soil improver is prepared from polymeric amino acid, humic acid, phosphogypsum, pyroligneous liquor, oxalic acid, a compound microbial agent and biochar. The compound microbial agent is obtained by mixing bacillus subtilis, pseudomonas, bacillus amyloliquefaciens and citrobacter Young. The invention further provides a method for improving the saline-alkali soil, the saline-alkali degree of the soil can be rapidly reduced, the granular structure is improved, the soil fertility is improved, and the yield and quality of crops in the saline-alkali soil are improved.

The invention discloses a plant stress-resistant nutrient solution mother liquor composition as well as a preparation method and application thereof. The plant stress-resistant nutrient solution mother liquor composition consists of a first stress-resistant mother liquor and a second stress-resistant mother liquor, the first stress-resistant mother liquor is formed by mixing a betaine aqueous solution and a carboxymethyl chitosan acetic acid solution; the second stress-resistant mother liquor is prepared by mixing a folium cortex eucommiae extract ethanol solution, a bacillus amyloliquefaciens fermentation supernatant and a nano-hydroxyapatite suspension according to a volume ratio of (1.5-2.5): (2.5-3.5): (0.8-1.2). The stress-resistant nutrient solution mother liquor composition is designed, multiple components including betaine, chitosan, eucommia ulmoides extract, bacillus amyloliquefaciens and nano-hydroxyapatite are synergistically compounded and applied through root irrigation, so that the stress resistance of crops is stably improved for a long time, and the defects that traditional foliage dressing is short in lasting effect, high in operation frequency and limited by the environment are effectively overcome.

The invention discloses a bifunctional xylanase fusion protein, a preparation method thereof and application of the bifunctional xylanase fusion protein in preparation of feruloyl oligosaccharide, and belongs to the technical field of genetic engineering and enzyme engineering. The fusion protein is composed of GH10 xylanase derived from bacillus altitudinis, a connecting peptide and GH11 xylanase derived from bacillus amyloliquefaciens. According to the invention, through an elaborately designed linker, the GH10 family xylanase and the GH11 family xylanase are fused through a specific connecting peptide, so that mutual interference between structural domains can be effectively overcome, the synergistic catalysis of GH10 and GH11 is fully played, and the novel bifunctional xylanase with synergistic interaction, strong specificity, high catalytic efficiency and high thermal stability is obtained. Compared with a single enzyme or a physical compound enzyme, the compound enzyme has better hydrolysis efficiency and application performance, and can be widely applied to the fields of food, textile, paper pulp bleaching, feed industry and the like.

This invention relates to a black rice fermentation powder, its preparation method, and its application, belonging to the field of food fermentation and processing technology. This invention provides a method for preparing black rice fermentation powder, comprising the following steps: (1) crushing black rice and sterilizing it to obtain black rice flour; (2) inoculating the black rice flour with a fermenting agent to obtain a fermentation substrate; (3) mixing the fermentation substrate with water for fermentation and freeze-drying to obtain black rice fermentation powder. This invention uses a specific combination of *Saccharomyces cerevisiae* and *Bacillus amyloliquefaciens* as a fermenting agent to ferment black rice flour. Through experiments, the optimal fermentation parameters were determined. The resulting black rice fermentation powder exhibits significantly higher levels of active ingredients and antioxidant capacity than unfermented and single-strain fermented products, fully verifying the advanced nature, synergy, and high efficiency of this application.

The invention relates to biological prevention and control of weeds and discloses an antagonistic microorganism combined fungicide for inhibiting growth of trifolium repens. The combined fungicide is prepared from the following active ingredients in percentage by weight: 30%-45% of bacillus amyloliquefaciens liquid, 25%-35% of pseudomonas fluorescens liquid, 20%-30% of trichoderma harzianum liquid and 5%-10% of bacillus subtilis liquid, the viable count of the bacillus amyloliquefaciens is greater than or equal to 1 * 10 < 9 > CFU / mL, the viable count of the pseudomonas fluorescens is greater than or equal to 5 * 10 < 8 > CFU / mL, the spore concentration of the trichoderma harzianum is greater than or equal to 2 * 10 < 7 > / mL, and the viable count of the bacillus subtilis is greater than or equal to 8 * 10 < 8 > CFU / mL. According to the antagonistic microorganism combined fungicide, through the synergistic antagonistic effect of bacillus amyloliquefaciens, pseudomonas fluorescens, trichoderma harzianum and bacillus subtilis, and in cooperation with a specific auxiliary agent and an adaptive use method, the antagonistic microorganism combined fungicide has the advantages that the antagonistic microorganism combined fungicide can be applied to lawn weed control, farmland intercropping and interplanting white clover prevention and control, garden green land white clover overgrowth treatment and other scenes; the growth of the trifolium repens can be effectively inhibited, and the plant height growth rate, the fresh weight and the coverage / density of the trifolium repens can be remarkably reduced.

The invention belongs to the technical field of microorganisms and baijiu brewing, and particularly relates to bacillus amyloliquefaciens capable of producing ethyl lactate and application of the bacillus amyloliquefaciens to improvement of fermented ethyl lactate of rice-flavor baijiu. The strain is separated from later-stage fermentation liquor of rice-flavor liquor, and has the characteristics of acid resistance, high-concentration ethanol resistance and high-yield ethyl lactate. According to the invention, the strain is added in the later period of fermentation of the rice-flavor liquor for enhanced fermentation, and then physical temperature control is carried out, so that the concentration of ethyl lactate can be synergistically increased, flavor substances such as total ester of the rice-flavor liquor can be remarkably increased, and meanwhile, the liquor yield is not influenced, so that the compound fragrance of rice wine is increased, and the liquor quality is improved. The method is good in stability among batches, can obtain the rice-flavor baijiu with high ethyl lactate content, and is suitable for popularization and application in baijiu brewing.

The invention discloses a plant source cadmium blocking agent and application thereof. According to the plant-source cadmium blocking agent provided by the invention, two-stage fermentation products of organic carbohydrates are taken as a core, and the plant-source cadmium blocking agent is prepared by synergistic fermentation of three bacteria, namely bacillus subtilis, bacillus licheniformis and bacillus amyloliquefaciens, and synergistic catalysis of three enzymes, namely cellulase, amylase and protease. And four auxiliaries including xanthan gum, sodium carboxymethyl cellulose, sodium alginate and Arabic gum are compounded to form a stable system. After the preparation is applied to leaf vegetables through rhizosphere irrigation, the effects are remarkable: (1) cadmium accumulation is reduced; (2) promoting growth and development; (3) enhancing stress resistance; and (4) improving the rhizosphere environment. Compared with a chemical passivator and an inorganic element preparation, the preparation provided by the invention has the advantages of synergism of growth promotion and cadmium resistance, easily available raw materials, simple and convenient process, environmental friendliness and the like, the treatment cost per mu is 50-80 yuan, the input-output ratio is more than 1: 3, and the preparation is suitable for large-scale safe production of leaf vegetables in alkaline cadmium-polluted soil in the north.

The invention provides a method for preparing chitosan with high deacetylation degree based on a microbial fermentation method. The preparation method comprises the following steps: preparing colloidal chitin turbid liquid; preparing a fermentation culture medium; transferring the prepared culture medium into a fermentation tank, sterilizing, cooling, and adjusting the pH value; inoculating an aspergillus oryzae seed solution and fermenting; inoculating bacillus amyloliquefaciens and fermenting; and carrying out sterilization, centrifugation, precipitate collection, washing, treatment with a NaOH solution, washing and freeze drying so as to obtain the product. The method comprises the following steps: by simulating a microorganism synergistic degradation process in nature and regulating and controlling fermentation conditions of aspergillus oryzae and bacillus amyloliquefaciens in stages, firstly completing wall breaking pretreatment by the aspergillus oryzae, carrying out mild and controllable enzymolysis, destroying crystal structures of the aspergillus oryzae and exposing a large amount of free amino groups and acetyl sites, so that the viscosity of fermentation liquor can be obviously reduced, and the fermentation liquor can be used for fermenting the bacillus amyloliquefaciens; extremely favorable conditions are created for the next-step deacetylation reaction, and deep and thorough deacetylation is realized.

The invention relates to the technical field of agriculture, and discloses a potato seed dressing agent for improving soil and a preparation method thereof.The potato seed dressing agent is composed of a component A, a component B, a component C and a component D. The component A comprises tannic acid, ferric ammonium citrate, citric acid, glycerin, chitosan oligosaccharide, caffeic acid and deionized water; the component B comprises bacillus subtilis powder, bacillus amyloliquefaciens powder, trehalose, betaine, L-proline and deionized water; the component C comprises charcoal powder, ammonium magnesium phosphate, potassium humate and potassium silicate powder; and the component D comprises ferulic acid and deionized water. By adopting a seed dressing mode that the component A, the component B, the component C and the component D are sequentially applied in use, the component A is sprayed in advance, and then the component B and the component C are applied under a rolling mixing condition, so that microorganisms and powder components can be captured and fixed on an adhesion base layer, and the risk that a medicament falls off from the surfaces of seed potatoes in the seed dressing process is reduced.

The invention discloses a walnut anthracnose control agent and a preparation method thereof, and belongs to the technical field of walnut disease control, and the control agent comprises bacillus amyloliquefaciens, sodium alginate, sodium cholate, mannitol, soya bean lecithin, xanthan gum and other components. Sodium alginate and crystalline mannitol are adopted to jointly prepare a porous carrier to load bacillus amyloliquefaciens, so that a sufficient and appropriate living space is provided for the bacillus amyloliquefaciens, and sodium cholate, xanthan gum and other components are combined to jointly act to shield the interference of external adverse environmental factors at the initial stage of applying a control agent; the bacillus amyloliquefaciens is ensured to enter the environment to play a disease control role after being massively and rapidly proliferated, the disease control effect is improved, and the control agent has a good application prospect in the field of walnut anthracnose control.