82 results about "Bacillus amylolyticus" patented technology

The invention relates to bacillus amyloliquefaciens NCPSJ7 and further relates to an application of the strain in treating plant diseases, diseases before and after harvesting fruits and vegetables as well as in preventing food-borne pathogenic bacteria and putrefying bacteria. The strain is preserved in China Center for Type Culture Collection (CCTCC) on March 22, 2013, wherein the preservation number is CCTCC NO: M2013098 and the strain is named as bacillus amyloliquefaciens NCPSJ7. The thallus and fermentation liquor of the strain disclosed by the invention has the effects of treating plant diseases caused by plant pathogenic fungus including antagonistic peach root rotten disease, fusarium wilt of cucumber, botrytis cinerea, jujube anthracnose, pear black spot, pear blue mould, apple brown rot, apple altermaria leaf spot, watermelon fusarium wilt and the like, as well as diseases after harvesting fruits and vegetables and food-borne pathogenic bacteria and putrefying bacteria including antagonistic staphylococcus aureus, salmonella paratyphi A, vibrio parahaemolyticus, yeast and the like; moreover, the bacillus amyloliquefaciens is broad in spectrum and antibacterial and great in potential in developing novel, efficient and natural biological control and biological preservative and fresh-keeping preparation.

The invention relates to a biological agent against crucifer club root and application thereof, belonging to the technical field of bio-pesticide. The strain for production is Bacillus subtilis XF-1, whose preserving number is CGMCC NO.2357. The stain has the characteristics as follows: (1) the primary colony on the LB culture substrate is white and round having a wet surface; the later colony is light yellow having uneven edge with dry and crimple surface; observed from the microscope, the strain is short-bar shaped and movable with spore, peritricha and dimension of 0.7 - 0.8 * 2.0-2.4 mum; (2) the strain is Gram-positive and aerobic and makes use of glycogen, sugar, citrate, gelatin hydrolysate, starch and casein, but does not make use of cellulose, tyrosine and catalase positive; (3) the stain has the function of sterilization, disease prevention, and yield improvement. The embodiment of the invention is as follows: using the test tube of Bacillus subtilis XF-1 stain, shake cultivation, and culture solution for fermentation to prepare biological agent, then applying the biological agent to the rhizosphere soil of crucifer crops, thereby having good effect in preventing and treating, and simple production.

The present invention relates to bacillus amyloliquefaciens and a preparation method of a microbial inoculum of the bacillus amyloliquefaciens, and belongs to the field of bio-pesticides and plant protection. The bacillus amyloliquefaciens HN-11 is isolated from azadirachta indica tree rhizosphere soil, azadirachta indica residue is used as a medium raw material and a microbial inoculum adsorption carrier for secondary solid state fermentation composting to prepare the microbial inoculums, the microbial inoculums comprises an arbitrary content of the bacillus amyloliquefaciens, 0 to 30% of the azadirachta indica residue, 4 to 12% of the soybean powder, 5 to 25% of wheat bran, 30-50% of peat, 1-5% of KCl, 0.5 to 2% of urea and 1 to 8% of a surfactant. Tests show that the microbial inoculum can overcome the problem of seedling injury caused by direct use of the azadirachta indica residue, can expand the control spectrum, has obvious integral control effect on banana wilt and Radopholus similes Thorne, can also promote plant growth, and the field control effect on the banana wilt can reach 96.2%. The microbial inoculum has great significance to the promotion of integral pest control and agricultural and sideline product recycling, and has broad application prospects in the production practice.

The invention provides a compound microbial agent, a bio-organic fertilizer as well as a preparation method and application, and belongs to the technical field of microbial agent organic fertilizer. The compound microbial agent comprises the following components in parts by mass: 1.5-2.5 parts of Bacillus amyloliquefaciens, 0.5-1 part of Bacillus licheniformis, 3-4 parts of Bacillus subtilis, 1.5-2 parts of Bacillus megaterium and 1.5-2.5 parts of Aspergillus oryzae. The compound microbial agent can ferment livestock and poultry manure quickly, and the fermentation only lasts for 10-15 days; and the compound microbial agent has a good deodorizing effect, and can quickly decompose crude fibers in livestock and poultry manure and effectively remove heavy metals in the livestock and poultry manure.

The present invention relates to a purple sweet potato feed of microbial fermentation, which is made of the fermentation from the microbial mother liquor together with the inoculated purple sweet potato mixture; wherein the strains for the microbial mother liquor is 400 to 500 ml of starch solution bacillus, 100 to 200 ml of condensation bacillus, 300 to 400 ml of lactobacillus acidophilus, 10 to90 ml of Candida utilis; the purple sweet potato comprises 14 to 34 percent of purple sweet potato, 10.7 to 18.7 percent of chili powder, 10 to 18 percent of chrysanthemum pollen, 0.3 to 0.6 percentof sodium hydroxide, 0.7 to 1.0 percent of ammonium sulfate, 40 to 52 percent of water. Other plant wastes from which the pigment has been extracted are added into the remaining purple sweet potato from which the pigment also has been extracted, and through the microbial fermentation which is transformed into the feeds for pigs, chickens and geese, which improves the palatability of the feed, increases feed enzyme components and decreases the cost, which is of reasonable design, simple operation, convenient production, and is suitable for the broad farmers and breeding plants.

The invention discloses bacillus amyloliquefaciens. The strain is preserved in the China General Microbiological Culture Collection Center on July 14th, 2015, and the preservation number is CGMCC No.11067. The invention further discloses a preparation method of the bacillus amyloliquefaciens. The invention further discloses protease produced by the bacillus amyloliquefaciens and further discloses an application of the bacillus amyloliquefaciens in tanning depilation. The protease produced by the bacillus amyloliquefaciens has good depilation activity in sheepskin, pigskin and cow leather which are frequently used in tanning, wherein the depilation effects on the cow leather and the sheepskin are the best, naked leather which is subjected to the depilation is soft, a softening effect is achieved, the grain surface is smooth, and pores are clear.

The invention provides a hermetia illucens compound amino acid fertilizer and a preparation method thereof.The preparation method comprises the steps that 1, smashing and drying dried hermetia illucens bodies, and stirring and mixing obtained powder and dried grape skin powder residues under hot air according to the mass ratio of (50-80): 1; (2) inoculating bacillus amyloliquefaciens Bt521 into aseed culture medium, and carrying out fermentation, so as to obtain a seed solution; (3) adding 50-60% of an obtained composite hermetia illucens powder into a fermentation medium; (4) inoculating 1-3% of the seed solution to the fermentation medium, and carrying out oscillation fermentation at 26-30 DEG C and 150-250 rpm for 72-78 h to obtain the hermetia illucens compound amino acid fertilizer;the prepared compound amino acid fertilizer has the advantages that the raw materials are rich in protein, the degradation rate of hermetia illucens is high, the fermentation period is short, the varieties of amino acids are rich, and the content of the amino acids can reach 0.32 mg/mL.

The invention discloses a seedling raising substrate for cultivating salt resistant plant seedlings, and a preparation method and application of the seedling raising substrate for cultivating salt resistant plant seedlings. The seedling raising substrate for cultivating salt resistant plant seedlings is prepared from mushroom residues, livestock and poultry excrement and urine, vinegar residues, gypsum residues, vermiculite, functional microorganisms and plant nutrient substances. The preparation method comprises the steps of preparing thoroughly decomposed mushroom residues through stacking fermentation of the mushroom residues, the livestock and poultry excrement and urine and the vinegar residues, preparing plant nutrition element enveloping bodies through entrapping potassium nitrate and magnesium sulphate with urea-formaldehyde resins, preparing microorganism enveloping bodies through entrapping functional microorganisms of trichoderma harzinum T83 and bacillus amyloliquefaciens IAE with polyglutamic acid, and preparing the substrate through solid fermentation and compounding of raw materials. The prepared substrate has stable high-cation environment, resistance of seedlings to hyperosmosis is continuously trained, and seedling raising substrate products suitable for heavy-salt soil regions are developed. The salinity of transplanted soil, which can be borne by plant seedlings cultivated by the products, is increased by 50% to 200%.

The invention provides microbial fermentation feed for procambarus clarkia. The feed comprises the following components: soybean meal, bean dregs, vegetable cake, corn meal, fish meal, kelp meal, an attractant, a microbial strain and microelements, wherein the attractant contains betaine and squid visceral powder, the microbial strain includes at least one of bacillus subtilis and bacillus amyloliquefaciens and aspergillus niger, and the microelements include iron, manganese, zinc, silicon, cobalt and boron. The feed has a reasonable formula, is rich in various nutrient ingredients such as protein, amino acids, vitamins, minerals and unsaturated fatty acids, and can effectively meet the growth needs of the procambarus clarkia, and microorganisms in the feed can hydrolyze organic matter ina water body through the own metabolic activities, thereby achieving the purpose of purifying water.

The invention discloses an ecological base stock for field planting engineering of a mangrove forest, a preparation method for the ecological base stock and application of the ecological base stock. The ecological base stock for field planting engineering of the mangrove forest is prepared from a porous material, peat, a functional microbe and decomposed manure of livestock and poultry, wherein aratio (in parts by mass) of the porous material to the decomposed manure of livestock and poultry to the peat is (1 to 5): (2 to 3): (5 to 10); and the addition level of the functional microbe accounts for 1.5%-3.5% the total mass of the ecological base stock. The functional microbe is bacillus amyloliquefaciens IAE. The porous material comprises ceramsite, biochar large granules and a macro-porosity lightweight substance; the peat comprises pearlite and coco coir; and the decomposed manure comprises fermented chicken manure and cattle manure. The ecological base stock is laid in holes of a tidal flat for transplanting young mangrove plants. According to the ecological base stock, pores of soil/deposit are increased, water exchange capacity of the deposit is improved, the oxygen content ofroot systems of the young mangrove plants is increased, and the survival rate and growth speed of the young mangrove plants are increased.

The invention provides a microbial soil improvement microbial agent for preventing and treating tobacco epidemic diseases. The microbial soil improvement microbial agent comprises bacillus amyloliquefaciens cd5bc rape straw fermentation products and streptomyces oxysporus WS-29246. The microbial soil improvement microbial agent comprises bacillus amyloliquefaciens cd5bc rape straw fermentation products and streptomyces oxysporus WS-29246, the preservation number of the bacillus amyloliquefaciens cd5bc is CCTCC (China Center For Type Culture Collection) NO: M 2016232; the preservation number of the streptomyces oxysporus WS-29246 is CCTCC (China Center for Type Culture Collection) NO: M 2012413; the bacillus amyloliquefaciens cd5bc rape straw fermentation product is prepared by taking rape straw as a fermentation substrate and inoculating bacillus amyloliquefaciens cd5bc as zymophyte for fermentation. The control effects of the composition on the tobacco black shank and the tobacco root black rot can reach 85% or above; cadmium accumulation in tobacco can be effectively reduced, lysine in the tobacco can be effectively reduced, the quality of the tobacco can be improved, harm to a human body is reduced, and the method has a good application prospect.

The invention belongs to the field of gene and biological fermentation, and discloses the application of ornithine cyclohexanase in improving the yield of ibuprofen A produced by bacillus amylolyticus. A plasmid pHY300PLK is used as a basis, by constructing an over-expression vector pHY-ocd of ornithine cyclohexanase Ocd from corynebacterium glutamicum, expression of a gene ocd is promoted in thebacillus amylolyticus LX-12 successfully, and bacillus amylolyticus engineered strains LX-12/pHY-ocd are obtained. Compared with control strains LX-12/pHY300, the yield of IturinA of the engineered strains LX-12/pHY-ocd is increased by at least 37%.

The invention provides a method for preparing a pesticide by fermenting polygonum orientale. The method is technologically characterized in that the polygonum orientale is used as a main raw material and is assisted by domestic garbage, starchy materials and water, commercially available bacillus pumilus, bacillus thuringiensis and starch hydrolyzing bacillus H47 are used as fermenting strains, pH is kept to be 5-8 according to the ratio of the polygonum orientale to the domestic garbage to the starchy materials to the purified water of (2-5): (1-2): 0.01: (3-7), the 2-5% strain inoculation quantity of a system, the fermentation temperature of 25-35 DEG C and the rotating speed is 100-200 rpm, and the fermentation time is 6-9 days. Complete fermentation is achieved, so that various enzymes, vitamins, amino acids and trace elements are fully released, and the efficacy and function of the pesticide are improved. In addition, the physiologically active substances of the plant pesticide are also not affected.

The invention relates to bacillus amyloliquefaciens KY09 and application thereof in deodorization. The classification name of the bacterial strain is Bacillus amyloliquefaciens KY09, the bacterial strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO:M 2020153. The strain can remove ammonia nitrogen and hydrogen sulfide at the same time, can effectively degrade odor generated by kitchen garbage fermentation, reduces odor pollution, and has the advantages of high efficiency and no need of additional energy consumption. When the strain is used for treating the odor of the kitchen garbage, the energy consumption in the resource utilization process of the kitchen garbage can be remarkably reduced, energy conservation and emission reductionare achieved, and more economic values are brought to resource utilization of the kitchen garbage.

The invention relates to the field of biological control and bacterial manure, in particular to bacillus amyloliquefaciens GLD-191, a microbial agent of the bacillus amyloliquefaciens GLD-191 and a preparation method and application of the bacillus amyloliquefaciens GLD-191. The bacillus amyloliquefaciens GLD-191 strain is deposited in the China General Microbiological Culture Collection Center onDecember 19, 2018, and the preservation number is CGMCC No.17011. The invention further provides the microbial agent prepared from the bacillus amyloliquefaciens GLD-191, a preparation method of themicrobial agent, and application of the bacillus amyloliquefaciens GLD-191 and the microbial agent thereof in the prevention and treatment of plant diseases. The bacillus amyloliquefaciens GLD-191 andan isatis root slag solid medium are subjected to secondary fermentation to prepare the novel microbial agent, the utilization rate of nutrients in isatis root slag is improved, and the bacillus amylolyticus GLD-191 can produce antibacterial substances with antimicrobial activity against plant pathogenic fungi, the population of beneficial microorganisms is increased, and the spectrum of diseasecontrol is expanded.

The invention discloses a fermentation broth processing method capable of improving final concentration of pullulanase, and belongs to the technical field of enzyme engineering and fermentation engineering. According to the fermentation broth processing method capable of improving final concentration of pullulanase, thermal insulation is carried out after mixing of a treatment solution C with a pullulanase fermentation liquid supernatant; and then, ultra-filtration concentration is carried out so as to obtain pullulanase fermentation broth with high enzyme activity. Being applied in post-extraction stage of pullulanase prepared by performing fermentation with recombinant bacillus amylolyticus BF7658/pUC980-BdP, the fermentation broth processing method is capable of improving ultrafiltration performance of the pullulanase fermentation broth. Being subjected to ultra-filtration concentration, the enzyme activity of the concentrate prepared from the fermentation broth liquid supernatant by the fermentation broth processing method disclosed by the invention is higher than 1.75 times of the enzyme activities of concentrates prepared from fermentation broths which are not yet treated with the treatment solution C after the same ultra-filtration concentration process.

The invention discloses bacillus amyloliquefaciens for inhibiting soy sauce from forming albuginea and application of the bacillus amyloliquefaciens, and belongs to the technical field of microorganisms. According to the invention, soy sauce mash microorganisms are separated and purified to obtain the strain bacillus amyloliquefaciens JDF-1 capable of inhibiting pichia pastoris or albuginea produced by pichia pastoris and inhibiting or eliminating formation of albuginea in soy sauce; and in a simulated soy sauce fermentation system, the bacillus amyloliquefaciens JDF-1 is added, so that generation of albuginea in the system can be inhibited. The bacillus amyloliquefaciens JDF-1 obtained by screening is suitable for a soy sauce fermentation production environment, and albuginea in soy sauce can be well generated by adjusting the addition amount of the strain, so that the bacillus amyloliquefaciens JDF-1 has important significance in reducing the generation of the albuginea and improving the quality of the soy sauce in the soy sauce fermentation production.