Plant stress-resistant nutrient solution mother liquor composition as well as preparation method and application thereof

Abstract

The invention discloses a plant stress-resistant nutrient solution mother liquor composition as well as a preparation method and application thereof. The plant stress-resistant nutrient solution mother liquor composition consists of a first stress-resistant mother liquor and a second stress-resistant mother liquor, the first stress-resistant mother liquor is formed by mixing a betaine aqueous solution and a carboxymethyl chitosan acetic acid solution; the second stress-resistant mother liquor is prepared by mixing a folium cortex eucommiae extract ethanol solution, a bacillus amyloliquefaciens fermentation supernatant and a nano-hydroxyapatite suspension according to a volume ratio of (1.5-2.5): (2.5-3.5): (0.8-1.2). The stress-resistant nutrient solution mother liquor composition is designed, multiple components including betaine, chitosan, eucommia ulmoides extract, bacillus amyloliquefaciens and nano-hydroxyapatite are synergistically compounded and applied through root irrigation, so that the stress resistance of crops is stably improved for a long time, and the defects that traditional foliage dressing is short in lasting effect, high in operation frequency and limited by the environment are effectively overcome.

Description

Technical Field

[0001] This invention relates to the field of plant nutrient solution technology, and in particular to a plant stress-resistance nutrient solution mother liquor composition, its preparation method, and its application. Background Technology

[0002] In recent years, the trend of extreme global climate change has intensified, and the negative impacts of abiotic stresses such as high temperatures, low temperatures, and drought on crop production have become increasingly prominent. Against this backdrop, although traditional nutrient solutions can provide basic nutrients, they generally lack systemic stress resistance functions, leading to physiological disorders such as membrane lipid peroxidation and decreased enzyme activity in crops under adverse conditions, resulting in significant yield reductions.

[0003] In the field of plant stress resistance technology, the application of exogenous stress-resistant substances is an important means to enhance crop resistance. Among them, stress resistance programs with betaine and chitosan as the main functional components have been widely used. Betaine, as a highly efficient osmotic regulator, can stabilize the structure of biological macromolecules and maintain cellular osmotic balance; chitosan can stimulate the plant's own immune response, promote root development, and has certain antibacterial activity. In current technologies, these stress-resistant substances are mostly formulated as foliar fertilizers and applied to crops through spraying. While this method can quickly replenish nutrients and show results in a short period, its drawbacks are also quite significant: First, foliar fertilizers have a short-lasting effect, requiring frequent application and incurring high labor costs; second, their effectiveness is strictly limited by environmental conditions, and cannot be applied in high temperature, high humidity, rainy, or windy weather, and may even cause phytotoxicity due to excessive evaporation or leaf burn from droplets; third, during critical growth stages of crops, such as the flowering and fruit enlargement stages, effective foliar spraying is often difficult to carry out due to canopy closure or to avoid physical damage to flowers and fruits, and at this time, the plant's nutritional needs are high, which cannot be met by foliar absorption alone.

[0004] Furthermore, existing stress-resistance programs often have single components and lack synergistic mechanisms, making it difficult to comprehensively address complex abiotic stresses. Therefore, there is an urgent need to develop a plant stress-resistance nutrient solution formula that can provide a stable and long-lasting supply through the root system, possesses multiple stress-resistance mechanisms, and is suitable for application in fertigation systems. Summary of the Invention

[0005] To address the aforementioned technical problems, the present invention aims to provide a plant stress-resistance nutrient solution stock solution composition, its preparation method, and its application. This invention designs a stress-resistance nutrient solution stock solution composition that synergistically combines multiple components, including betaine, chitosan, Eucommia ulmoides extract, Bacillus amyloliquefaciens, and nano-hydroxyapatite. Applied via root irrigation, this achieves a long-lasting and stable enhancement of crop stress resistance, effectively overcoming the shortcomings of traditional foliar fertilization, such as short-lasting effects, frequent operation, and susceptibility to environmental constraints.

[0006] To achieve the above-mentioned technical objectives and effects, the present invention is implemented through the following technical solution: The present invention provides a plant stress-resistant nutrient solution mother liquor composition, which is composed of a first stress-resistant mother liquor and a second stress-resistant mother liquor; the first stress-resistant mother liquor is composed of a mixture of betaine aqueous solution and carboxymethyl chitosan acetate solution; the second stress-resistant mother liquor is composed of a mixture of ethanol solution of Eucommia ulmoides leaf extract, fermentation supernatant of Bacillus amyloliquefaciens and nano hydroxyapatite suspension in a volume ratio of (1.5~2.5):(2.5~3.5):(0.8~1.2).

[0007] Furthermore, the concentration of betaine in the betaine aqueous solution is 800-1200 ppm, and the concentration of carboxymethyl chitosan in the carboxymethyl chitosan acetate solution is 18000-22000 ppm; in the first anti-stress mother liquor, the mixing volume ratio of the betaine aqueous solution and the carboxymethyl chitosan acetate solution is 1:1.

[0008] Furthermore, the concentration of Eucommia ulmoides leaf extract in the ethanol solution is 4500~5500 ppm; and the concentration of nano-hydroxyapatite in the nano-hydroxyapatite suspension is 800~1200 ppm.

[0009] Furthermore, the betaine is agricultural grade with a purity of ≥90%; the carboxymethyl chitosan has a degree of deacetylation of ≥85%.

[0010] Furthermore, the chlorogenic acid content in the Eucommia ulmoides leaf extract is ≥12%, and the eucommia gum content is ≥5%.

[0011] Furthermore, the volume ratio of the first anti-stress mother liquor to the second anti-stress mother liquor is 5:1.

[0012] Another aspect of the present invention provides a method for preparing the plant stress-resistance nutrient solution mother liquor composition, comprising the following steps: (1) Preparation of the first stress-resistant mother liquor: dissolve betaine in water to prepare a betaine aqueous solution; dissolve carboxymethyl chitosan in acetic acid solution to prepare a chitosan acetic acid solution; mix the two solutions to obtain the first stress-resistant mother liquor; (2) Preparation of the second stress-resistant mother liquor: Eucommia ulmoides leaf extract was dissolved in ethanol solution to prepare Eucommia ulmoides leaf extract ethanol solution; Bacillus amyloliquefaciens fermentation broth was filtered and the supernatant was retained; nano-hydroxyapatite was ultrasonically dispersed in deionized water to prepare nano-hydroxyapatite suspension; the above Eucommia ulmoides leaf extract ethanol solution, Bacillus amyloliquefaciens fermentation supernatant and nano-hydroxyapatite suspension were mixed in a certain volume ratio to obtain the second stress-resistant mother liquor; (3) Preparation of plant stress resistance nutrient solution mother liquor composition: The first stress resistance mother liquor and the second stress resistance mother liquor are mixed in a certain proportion to obtain the plant stress resistance nutrient solution mother liquor composition.

[0013] The present invention also provides the application of the plant stress resistance nutrient solution stock solution composition in enhancing plant stress resistance, specifically: the plant stress resistance nutrient solution stock solution composition is diluted and mixed with diluted conventional nutrient solution, and applied through root irrigation.

[0014] In the above applications, the compounding and application process is achieved through an integrated water and fertilizer system.

[0015] In the above applications, the plant stress-resistance nutrient solution stock solution composition is applied to the root system by drip irrigation during the flowering or fruit-expanding stage of the crop.

[0016] The beneficial effects of this invention are as follows: This invention separates components with significantly different or potentially interdependent physicochemical properties into two mother liquors, thereby preventing precipitation, inactivation, or degradation of the components during high-concentration storage and ensuring the stability of the mother liquor composition during storage.

[0017] The plant stress-resistance nutrient solution mother liquor composition of the present invention achieves synergistic effects of multiple stress-resistance mechanisms. The first stress-resistance mother liquor (betaine + carboxymethyl chitosan) provides basic osmotic regulation and immune stimulation functions. Betaine, as an osmotic regulator, can maintain cellular osmotic balance and stabilize protein and enzyme activity, thereby enhancing cellular tolerance to stress; carboxymethyl chitosan can stimulate plant resistance and promote root growth, enhancing nutrient absorption capacity. The second stress-resistance mother liquor (Eucommia ulmoides leaf extract + bacterial metabolites + nanomaterials) provides enhanced antioxidant, microbial regulation, and nutrient transport functions. Eucommia ulmoides leaf extract (rich in chlorogenic acid) synergistically enhances antioxidant capacity and inhibits membrane lipid peroxidation with betaine; the fermentation supernatant of Bacillus amyloliquefaciens is rich in active metabolites, which can improve rhizosphere microecology, promote the establishment of beneficial microbial communities, and further enhance the plant's systemic resistance; nano-hydroxyapatite not only provides phosphorus and calcium nutrition itself, but its unique adsorption and nano-effects may also serve as a carrier, improving the stability and targeted delivery efficiency of other active ingredients.

[0018] When using this mother liquor composition, it can be used as an independent module with a water and fertilizer machine. When using it, it should be diluted according to the ratio and mixed with conventional nutrient solution. It can be applied once through root irrigation, which greatly simplifies the operation and realizes the simplification and long-term effectiveness of stress management, effectively overcoming many drawbacks of traditional foliar fertilization methods. Detailed Implementation

[0019] The technical solutions of the present invention will be clearly and completely described below with reference to specific embodiments. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0020] This invention provides a plant stress-resistant nutrient solution mother liquor composition, which is composed of a first stress-resistant mother liquor and a second stress-resistant mother liquor; the first stress-resistant mother liquor is formed by mixing betaine aqueous solution and carboxymethyl chitosan acetate solution; the second stress-resistant mother liquor is formed by mixing Eucommia ulmoides leaf extract ethanol solution, Bacillus amyloliquefaciens fermentation supernatant and nano hydroxyapatite suspension in a volume ratio of (1.5~2.5):(2.5~3.5):(0.8~1.2).

[0021] The betaine aqueous solution contains 800-1200 ppm betaine, and the carboxymethyl chitosan acetate solution contains 18000-22000 ppm carboxymethyl chitosan. In the first stress-resistant mother liquor, the mixing volume ratio of the betaine aqueous solution to the carboxymethyl chitosan acetate solution is 1:1. The betaine is agricultural grade with a purity ≥90%, and the degree of deacetylation of the carboxymethyl chitosan is ≥85%.

[0022] The concentration of Eucommia ulmoides leaf extract in the ethanol solution was 4500-5500 ppm; the concentration of nano-hydroxyapatite in the nano-hydroxyapatite suspension was 800-1200 ppm. The Eucommia ulmoides leaf extract contained ≥12% chlorogenic acid and ≥5% eucommia gum.

[0023] In this plant stress-resistance nutrient solution mother liquor composition, the volume ratio of the first stress-resistance mother liquor to the second stress-resistance mother liquor is 5:1.

[0024] The preparation method of this plant stress-resistance nutrient solution mother liquor composition includes the following steps: (1) Preparation of the first stress-resistant mother liquor: dissolve betaine in water to prepare a betaine aqueous solution; dissolve carboxymethyl chitosan in acetic acid solution to prepare a chitosan acetic acid solution; mix the two solutions to obtain the first stress-resistant mother liquor; (2) Preparation of the second stress-resistant mother liquor: Eucommia ulmoides leaf extract was dissolved in ethanol solution to prepare Eucommia ulmoides leaf extract ethanol solution; Bacillus amyloliquefaciens fermentation broth was filtered and the supernatant was retained; nano-hydroxyapatite was ultrasonically dispersed in deionized water to prepare nano-hydroxyapatite suspension; the above Eucommia ulmoides leaf extract ethanol solution, Bacillus amyloliquefaciens fermentation supernatant and nano-hydroxyapatite suspension were mixed in a certain volume ratio to obtain the second stress-resistant mother liquor; (3) Preparation of plant stress resistance nutrient solution mother liquor composition: The first stress resistance mother liquor and the second stress resistance mother liquor are mixed in a certain proportion to obtain the plant stress resistance nutrient solution mother liquor composition.

[0025] This plant stress-resistance nutrient solution stock solution composition can be used to enhance plant stress resistance. Specifically, the stock solution composition is diluted and mixed with diluted conventional nutrient solution, then applied through root irrigation. This mixing and application process is achieved through an integrated water and fertilizer system.

[0026] In the above applications, the plant stress-resistance nutrient solution stock solution composition is applied to the root system by drip irrigation during the flowering or fruit-expanding stage of the crop.

[0027] Example 1. Preparation of mother liquor for the integrated water and fertilizer machine and setup of the fertilization system: 1.1 Prepare a mixed solution of 1000ppm betaine aqueous solution and 20000ppm chitosan acetic acid solution: 12.5g betaine + 12.5L water, 250g chitosan + 12.5L 1% acetic acid solution. The 25L nutrient solution obtained by mixing the two is called stress-resistant mother liquor C1 (first stress-resistant mother liquor).

[0028] 1.2 Mix 1666 ml of Eucommia ulmoides extract ethanol solution (Eucommia ulmoides leaf extract concentration of 5000 ppm), 2500 ml of Bacillus amyloliquefaciens fermentation supernatant, and 834 ml of nano-hydroxyapatite suspension (nano-hydroxyapatite concentration of 1000 ppm) at room temperature for 20 minutes with a stirring speed of 200 r / min to obtain 5 L of second stress-resistant mother liquor, denoted as stress-resistant mother liquor C2.

[0029] 1.3 C1 and C2 were diluted 10 times and then mixed to obtain an anti-stress nutrient solution C3 with a total volume of 300L.

[0030] 1.4 Prepare conventional nutrient solutions for leafy vegetables. Use the modified Hogland nutrient solution formula to prepare 300L of conventional nutrient stock solution A and 300L of conventional nutrient stock solution B. Conventional nutrient stock solution A is prepared by adding 22.98kg of nitrogen-potassium fertilizer [Ca(NO3)2·4H2O] + 0.74kg of chelated iron (Fe-EDTA) + 14.78kg of nitrogen-potassium fertilizer (KNO3) + 3.13kg of urea [CO(NH2)2] + 300L of water. The conventional nutrient stock solution B is prepared using 4.16 kg of potassium dihydrogen phosphate (KH2PO4) + 0.429 kg of potassium sulfate (K2SO4) + 1.13 kg of nitrogen and magnesium fertilizer [Mg(NO3)2·4H2O] + 10.62 kg of magnesium sulfate (MgSO4·7H2O) + 0.11 kg of borax (Na2B4O7·10H2O) + 8.84 g of zinc sulfate (ZnSO4·7H2O) + 68.19 g of manganese sulfate (MnSO4·H2O) + 7.09 g of copper sulfate (CuSO4·5H2O) + 7.33 g of sodium molybdate (Na2MoO4·2H2O) + 300 L of water.

[0031] 1.5 Fertilization Setup: For the integrated water and fertilizer machine channel one: Regular nutrient solution A and B are diluted 100 times each for drip irrigation; stress-resistant nutrient solution C3 is diluted 10 times for drip irrigation. Fertilization time is 8:00 AM daily, with 500ml of the mixture of regular nutrient solution A, B, and C3 dripped per plant; at 4:00 PM, 200ml of clean water dripped per plant. For the integrated water and fertilizer machine channel two: Regular nutrient solution A and B are diluted 100 times each for drip irrigation. Fertilization time is 8:00 AM daily, with 500ml of the mixture of regular nutrient solution A and B dripped per plant; at 4:00 PM, 200ml of clean water dripped per plant. 2. Crop preparation (1) Seedling raising: 288-cell trays were used to raise seedlings of the "Dasusheng" variety of lettuce. Two trays were used at a time, and the seedling raising period was 7 days.

[0032] (2) Transplanting was carried out when the seedlings had 3 leaves after 7 days of seedling cultivation. The control group CK group had 3 replicates, with 40 seedlings in each replicate, for a total of 120 seedlings. The drip irrigation operation in step 1.5 above was carried out by channel 2 of the integrated water and fertilizer machine. At the same time, the experimental group C group had 3 replicates, with 40 seedlings in each replicate, for a total of 120 seedlings. The drip irrigation operation in step 1.5 above was carried out by channel 1 of the integrated water and fertilizer machine.

[0033] (3) It is grown in a glass greenhouse with an average daytime temperature of 35°C and an average nighttime temperature of 28°C. No additional foliar fertilizer or pesticides are applied during the growth period.

[0034] 3. Data Recording (1) Leaf morphology index: ① Plant type index, plant height / plant width ratio, measure the height of the highest point of the plant (plant height) and the maximum spread width (plant width) with a ruler. A plant height / plant width ratio ≤ 0.5 is excellent; ② Leaf length-width ratio, the ratio of the longest part of the leaf to the widest part of the leaf, which reflects the degree of leaf narrowness and elongation. The ideal value is 1.5~2.0. (2) Leaf gloss and color index: ① Leaf gloss: The gloss unit (GU) of the leaf surface is measured by a gloss meter (60° incident angle). The higher the value, the stronger the reflection. The standard is 10~20 GU for fresh leaves, and the gloss of wilted or diseased leaves drops to below 5 GU; ② Scan the leaves with a multispectral imaging system and calculate the color uniformity of the whole leaf (standard deviation SD≤5). (3) Structural integrity index: ① The percentage of damaged leaves is counted. Damage is defined as leaf area missing > 5%. The evaluation criteria are shown in Table 1. ② Vein clarity: Under transmitted light, observe the number of branches of the main vein and secondary veins, and record them in grades. One main vein and ≥ 5 pairs of secondary veins are considered excellent.

[0035] Table 1 (4) Indicators of stress resistance: ① Relative water content of leaves, measured by RWC weighing method; ② Antioxidant enzyme activity was detected by spectrophotometry.

[0036] 4. Results Over a 21-day growth period (calculated from transplanting), under continuous high-temperature stress, the experimental group (C group) receiving stress-resistant nutrient solution C3 plus conventional nutrient solution and the control group (CK group) receiving conventional nutrient solution only showed significant differences in multiple indicators. Specific experimental results are as follows: (1) Leaf morphology indicators: Plant type index (plant height / plant width): Group C: The average value was 0.48 ± 0.03. The plants were short and sturdy, with good leaf spread and a compact plant shape, which was better than the standard of ≤0.5.

[0037] CK group: The average value was 0.62 ± 0.05. The plants showed a tendency to grow excessively, with slightly elongated stems and a looser plant shape, in order to compete for more space for heat dissipation.

[0038] Leaf length-to-width ratio: Group C: The average value is 1.8 ± 0.2. The leaf shape is regular and meets the ideal standard of 1.5~2.0.

[0039] CK group: The average value was 2.3 ± 0.3. The leaves were noticeably narrow and elongated, which is a typical manifestation of poor growth under adverse conditions.

[0040] (2) Leaf gloss and color indicators: Leaf gloss (GU): Group C: The average value was 16.5 ± 1.5 GU. The waxy layer on the leaf surface was intact, and the reflectivity was strong, which was within the standard range (10~20 GU) for fresh leaves.

[0041] CK group: The average value was 10.2 ± 2.0 GU. The leaves were slightly damaged and dehydrated due to high temperature stress, resulting in lower gloss compared to the C group.

[0042] Leaf color uniformity (standard deviation SD): Group C: The standard deviation of color difference across the entire leaf is 3.8. The color is uniform, the leaves are dark green, and there is no obvious yellowing or spots.

[0043] CK group: The standard deviation of color difference across the entire leaf is 5.5. Uneven coloring, with slight yellowing and scorching symptoms visible on the leaf edges or tips.

[0044] (3) Structural integrity index: Percentage of damaged blades: Group C: The damage rate was only 1.5%. The vast majority of leaves were intact, with no scorching at the edges and no visible damage to the naked eye, and were rated as "excellent".

[0045] Group CK: Damage rate was 4.2%. Some leaves showed edge scorching and minor damage, and were rated as "good".

[0046] Leaf vein clarity: Group C: The main veins of all tested leaves were clearly visible, and the average number of secondary veins was 6-7 pairs. The vascular system was well developed and the nutrient transport capacity was strong. The rating was "excellent".

[0047] Group CK: Although the main vein is clear, the number of secondary vein branches is small, averaging only 3-4 pairs, and the veins are slightly blurred.

[0048] (4) Physiological indicators of stress resistance: Relative water content of leaves (RWC): Group C: 85.2% ± 2.1%. This indicates that the plant has an extremely strong water retention capacity, effectively maintaining cell turgor pressure and resisting water evaporation caused by high temperatures.

[0049] CK group: 78.5% ± 3.8%. Leaves showed wilting, softening, and reduced luster, with increased fiber affecting lettuce quality.

[0050] Antioxidant enzyme activity (SOD, superoxide dismutase): Group C: Enzyme activity was 356.7 U / g FW (fresh weight). Compared with the CK group, the activity was significantly increased (p<0.01), indicating that the plant's ability to scavenge harmful free radicals was greatly enhanced, effectively reducing membrane lipid peroxidation damage.

[0051] CK group: Enzyme activity was 215.3 U / g FW. The enzyme activity level was low and insufficient to completely remove the large amount of reactive oxygen species generated by stress, leading to cell membrane damage.

[0052] The data in this embodiment fully demonstrate that the plant stress-resistance nutrient solution stock solution composition (C3) provided by this invention, applied through root irrigation, can significantly enhance the overall resistance of lettuce to high-temperature stress. Compared with the control group using only conventional nutrients, the experimental group showed statistically significant advantages in morphology, leaf health, structural integrity, and key physiological stress resistance. This composition effectively alleviates growth inhibition and oxidative damage caused by high-temperature stress, enabling the crop to maintain excellent marketable traits and physiological functions, thus achieving the design objective of stress-resistance management through an integrated water and fertilizer system.

[0053] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within the present invention.

[0054] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A plant stress tolerance nutrient solution stock composition, characterized in that, The first anti-stress mother liquor is prepared by mixing betaine aqueous solution with carboxymethyl chitosan acetic acid solution; the second anti-stress mother liquor is prepared by mixing eucommia leaf extract ethanol solution, bacillus amyloliquefaciens fermentation supernatant and nano-hydroxyapatite suspension according to the volume ratio (1.5-2.5):(2.5-3.5):(0.8-1.2).

2. The plant stress nutrition mother liquor composition according to claim 1, characterized in that, The concentration of betaine in the betaine aqueous solution is 800-1200 ppm, and the concentration of carboxymethyl chitosan in the carboxymethyl chitosan acetic acid solution is 18000-22000 ppm; in the first anti-stress mother liquor, the volume ratio of betaine aqueous solution to carboxymethyl chitosan acetic acid solution is 1:

1.

3. The plant stress nutrition mother liquor composition according to claim 1 or 2, characterized in that, The concentration of eucommia leaf extract in the eucommia leaf extract ethanol solution is 4500-5500 ppm, and the concentration of nano-hydroxyapatite in the nano-hydroxyapatite suspension is 800-1200 ppm.

4. The plant stress nutrition mother liquor composition according to claim 1, characterized in that, The betaine is agricultural grade, and the purity is greater than or equal to 90%; the degree of deacetylation of the carboxymethyl chitosan is greater than or equal to 85%.

5. The plant stress nutrition mother liquor composition according to claim 1, characterized in that, In the eucommia leaf extract, the content of chlorogenic acid is greater than or equal to 12%, and the content of eucommia gum is greater than or equal to 5%.

6. The plant stress nutrition mother liquor composition according to claim 1, characterized in that, The volume ratio of the first anti-stress mother liquor to the second anti-stress mother liquor is 5:

1.

7. A method of preparing a plant stress tolerance nutrient solution stock composition according to any one of claims 1 to 6, characterized by, The method comprises the following steps: (1) preparing the first anti-stress mother liquor: dissolving betaine in water to prepare betaine aqueous solution; dissolving carboxymethyl chitosan in acetic acid solution to prepare chitosan acetic acid solution; mixing the two solutions to obtain the first anti-stress mother liquor; (2) preparing the second anti-stress mother liquor: dissolving eucommia leaf extract in ethanol solution to prepare eucommia leaf extract ethanol solution; filtering bacillus amyloliquefaciens fermentation broth to retain the supernatant; dispersing nano-hydroxyapatite in deionized water by ultrasonic to prepare nano-hydroxyapatite suspension; mixing the eucommia leaf extract ethanol solution, bacillus amyloliquefaciens fermentation supernatant and nano-hydroxyapatite suspension according to a certain volume ratio to obtain the second anti-stress mother liquor; (3) preparing the plant anti-stress nutrient solution mother liquor composition: mixing the first anti-stress mother liquor and the second anti-stress mother liquor according to a certain ratio to obtain the plant anti-stress nutrient solution mother liquor composition.

8. Use of a plant stress tolerance nutrient solution mother liquor composition according to any one of claims 1 to 6 for enhancing plant stress tolerance, characterized in that, The plant anti-stress nutrient solution mother liquor composition is diluted and compounded with the diluted conventional nutrient solution, and is applied by root irrigation.

9. Use according to claim 8, characterized in that, The compounding and application process is realized by a water and fertilizer integrated system.

10. Use according to claim 8, characterized in that, The plant anti-stress nutrient solution mother liquor composition is applied to the root system by drip irrigation during the flowering period or fruit swelling period of crops.

Images