17 results about "Trichothecene" patented technology

The invention relates to a method for improving fermentation production efficiency of bacillus subtilis lipopeptide sodium by controlling threonine flow acceleration rate based on fermentation process oxygen uptake rate curve feedback. Bacillus subtilis is used as a production strain, and lipopeptide sodium is produced through deep liquid state fermentation. In the fermentation process, the oxygen uptake rate of the process is detected, a threonine and ammonium sulfate continuous feeding control process based on oxygen uptake rate curve parameters is implemented, the oxygen uptake rate in the fermentation process is stably maintained to be 60-65 mmo1 / L / h, the ammonium ion concentration is maintained to be 15-20 mmol / L, and rapid growth of hyphae and rapid increase of the lipopeptide sodium synthesis rate can be achieved. The threonine and ammonium sulfate fed-batch control process based on oxygen uptake rate feedback control is established, the production period is shortened, and the fermentation production level is stably improved.

The invention relates to nitrate reduction pelagic bacteria and application thereof. The nitrate reductive ocean bacillus can be fermented to produce pyrroloquinoline quinone (PQQ) and trichothecenes toxin degrading enzyme, the trichothecenes toxin degrading enzyme mainly exists in bacterial cell lysis buffer, and the degradation rate of the trichothecenes toxin degrading enzyme to vomitoxin is 83%. The bacterial strain fermentation liquor can be used for degrading 39.3 percent of nivalenol and 100 percent of 15-acetyl deoxynivalenol. The strain or the trichothecene toxin degrading enzyme produced by the strain can be used for complete feed, concentrated feed, premixed feed, feed raw materials such as grain, corn, peanut meal, wheat and oat, silage, grain, grain and oil, grain processing and agricultural and sideline products, aged grain, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour, corn flour and corn flour. Through biodegradation of industrial ethanol processing byproducts and trichothecenes in food, the quality safety of feed and food can be improved.

The present application discloses a polypeptide with catalytic activity of removing epoxy group, its coding nucleic acid and use. The polypeptide of the present application can catalyze the reaction of removing epoxy group between trichothecene and glutathione under mild conditions, and produce nontoxic and harmless glutathione derivative, so as to realize the detoxification and detoxication of trichothecene. The polypeptide of the present application has wide use in the fields of agriculture, food, feed and medicine.

The present invention relates to a composition, a method for reducing adverse effects of a trichothecene, use of a compound, and a compound for use in treatment, amelioration and / or prevention of symptom(s) associated with a trichothecene.

The application discloses a strain of completely degrading deoxynivalenol (DON), the N25 Nocardioides, the preservation number of the strain being CCTCC M 20252560. Average nucleotide identity (ANI) analysis shows that the strain is a new species in the Nocardioides genus, the strain can utilize DON toxin as a sole carbon source, and can completely degrade the DON toxin to form CO2 and H2O, completely eliminating the toxic effect, and can also degrade a plurality of fusarium toxins including A-type trichothecene toxin neosolensaric acid (NEO) and B-type trichothecene toxin 3-acetyl-DON (3-Ac-DON), nivalenol (NIV) and fusarenon-X (FUS-X). Therefore, the strain has a wide application prospect in the field of fusarium toxin detoxification.

The present invention relates to a method of converting 3-keto-DON into 3-epi-DON, reducing the content of DON in a composition comprising DON or of reducing the toxicity of a composition comprising DON as well as a method for converting a trichothecene comprising a 3-oxo group into a trichothecene comprising a 3-hydroxy group using one or more polypeptide(s) comprising or consisting of SEQ ID NO. 1 or a sequence having a sequence identity of at least 72.0% to SEQ ID NO. 1. Also envisioned are feed or food additives or feed or food as well as pharmaceutical compositions comprising one or more polypeptide(s) comprising or consisting of a sequence of SEQ ID NO. 1 or a sequence having a sequence identity of at least 72.0% to SEQ ID NO. 1 as well as the manufacture thereof. Encompassed are further polypeptide(s) comprising or consisting of a sequence of SEQ ID NO. 1 or a sequence having a sequence identity of at least 88.5% to SEQ ID NO. 1. Also envisioned are host cells or plants.

A method of reducing the toxicity of a trichothecene mycotoxin such as deoxynivalenol (DON) comprises exposing the trichothecene mycotoxin to a UDP- glycosyltransferase enzyme originating from a strain of Bacillus subtilis. The enzyme catalyzes the conversion of the trichothecene mycotoxin to a non-toxic monoglycosylated metabolite. The enzyme is useful in reducing the toxicity of animal feed or food products which would otherwise be contaminated with the trichothecene mycotoxin.

The application discloses a heterocyclic piperazine truncated pleuromutilin derivative and application thereof, relates to the field of piperazine truncated pleuromutilin derivatives and application thereof, and the heterocyclic piperazine truncated pleuromutilin derivative comprises a compound or a stereoisomer or a pharmaceutically acceptable salt thereof as shown in a structural formula of formula I.The heterocyclic piperazine truncated pleuromutilin derivative of the application shows excellent antibacterial effects on gram-positive bacteria, such as Staphylococcus aureus drug-resistant strains ATCC 33591 and ATCC 43300, Staphylococcus aureus sensitive strains ATCC 29213, Staphylococcus epidermidis drug-resistant strains ATCC 51625 and Staphylococcus epidermidis sensitive strains ATCC 12228.

The invention belongs to the technical field of agricultural biology, and particularly relates to aldoketoreductase ZHAKR8 as well as a coding gene, a preparation method and application of the aldoketoreductase ZHAKR8. The amino acid sequence of the aldehyde ketoreductase ZHAKR8 provided by the invention is as shown in SEQ ID NO. 1, and the nucleotide sequence of the coding gene of the aldehyde ketoreductase ZHAKR8 is as shown in SEQ ID NO. 2. According to the aldoketoreductase provided by the invention, the 3-ketone-vomitoxin can be converted into the 3-epivomitoxin, the speed of degrading the 3-ketone-vomitoxin is high, and the toxicity of the product 3-epivomitoxin is greatly reduced. The method has huge application prospects in the fields of feed, food, Chinese herbal medicine, biomass energy production and the like.

The present invention relates to methods of modifying trichothecene, mutant variants of polypeptides, additives, methods of manufacture, uses of said polypeptides, polynucleotides, recombinant host cells and polypeptides for treating, ameliorating and / or preventing symptoms caused by fungal poisoning.

The present invention relates to a method for modifying trichothecene, a composition comprising at least one polypeptide having at least 70% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO. 1 to SEQ ID NO. 54, and the use of said polypeptide.

The present invention relates to novel methods for detoxifying trichothecene-contaminated materials. More specifically, the present invention relates to a method for bioconverting trichothecenes by contacting trichothecene-contaminated materials with an exogenous, non-animal glutathione-S-transferase (GST) that has substrate specificity for the epoxy ring of trichothecenes. The present invention also relates to recombinant GST and transgenic plants and animals expressing said GST.

The invention belongs to the field of gene engineering, and particularly relates to application of BcGLOA protein in regulation and control of type-B trichothecenes, the BcGLOA protein can reduce accumulation of the type-B trichothecenes in plants, and the accumulation amount of the type-B trichothecenes in crop grains is reduced. Based on detoxification of B-type trichothecene toxins, toxin accumulation in crops is reduced, a specific catalytic mechanism is achieved, and the influence on other non-pathogenic bacteria is small.

The invention discloses a degrading enzyme ZHDDH4 with activity of degrading trichothecene toxin as well as a mutant and application of the degrading enzyme ZHDDH4. The invention firstly provides the trichothecene toxin degrading enzyme ZHDDH4, and the amino acid sequence of the trichothecene toxin degrading enzyme ZHDDH4 is shown as SEQ ID No. 1 or SEQ ID No. 2. According to the invention, the trichothecenes toxin degrading enzyme ZHDDH4 is further subjected to single-site mutation of any one of amino acids of A43M, N95M, H140F, G278L or D515R to obtain a single-site mutant. The trichothecenes toxin degrading enzyme ZHDDH4 or each mutant provided by the invention has relatively high activity of degrading the trichothecenes toxin, and can be used for biodegradation of the trichothecenes toxin in various biological matrixes; the method has a huge application prospect in the fields of enzyme preparation preparation, feed, food, Chinese herbal medicine, biomass energy production and sewage treatment.

The present invention provides a method for preparing an adsorbent for efficiently enriching multiple mycotoxins in cereals. The method adopts polydopamine and 1-carboxyethyl-3-butylimidazolium bromide dual-modified magnetic nanoparticles as a novel adsorbent to enrich multiple types of mycotoxins in cereals, such as aflatoxins, ochratoxins, fumonisins, zearalenone toxins, penicillin toxins, sterigmatocystin, and Class A and Class B trichothecenes toxins. The method reduces the co-adsorption of matrix components in the cereals, and provides a sample to be tested in which the target is enriched and interference is purified for subsequent analysis and detection.

Provided herein are Drug-Linkers and Antibody-Drug Conjugates comprising a Trichothecene for the use of treating diseases, disorders, or conditions.

Use of an alcohol dehydrogenase of SEQ ID No. 1 containing a metal ion and a quinone cofactor, or a functional variant thereof showing at least 80 %, preferably at least 86 %, especially preferably at least 89 % sequence identity and at least one redox cofactor, for the conversion of at least one trichothecene compound presenting a hydroxyl group at the C-3 atom, as well as methods for the enzymatic conversion of trichothecene compounds and trichothecene conversion additives.