9 results about "Trichothecene" patented technology

The invention relates to a method for improving fermentation production efficiency of bacillus subtilis lipopeptide sodium by controlling threonine flow acceleration rate based on fermentation process oxygen uptake rate curve feedback. Bacillus subtilis is used as a production strain, and lipopeptide sodium is produced through deep liquid state fermentation. In the fermentation process, the oxygen uptake rate of the process is detected, a threonine and ammonium sulfate continuous feeding control process based on oxygen uptake rate curve parameters is implemented, the oxygen uptake rate in the fermentation process is stably maintained to be 60-65 mmo1 / L / h, the ammonium ion concentration is maintained to be 15-20 mmol / L, and rapid growth of hyphae and rapid increase of the lipopeptide sodium synthesis rate can be achieved. The threonine and ammonium sulfate fed-batch control process based on oxygen uptake rate feedback control is established, the production period is shortened, and the fermentation production level is stably improved.

The present application discloses a polypeptide with catalytic activity of removing epoxy group, its coding nucleic acid and use. The polypeptide of the present application can catalyze the reaction of removing epoxy group between trichothecene and glutathione under mild conditions, and produce nontoxic and harmless glutathione derivative, so as to realize the detoxification and detoxication of trichothecene. The polypeptide of the present application has wide use in the fields of agriculture, food, feed and medicine.

The present invention relates to a composition, a method for reducing adverse effects of a trichothecene, use of a compound, and a compound for use in treatment, amelioration and / or prevention of symptom(s) associated with a trichothecene.

The application discloses a strain of completely degrading deoxynivalenol (DON), the N25 Nocardioides, the preservation number of the strain being CCTCC M 20252560. Average nucleotide identity (ANI) analysis shows that the strain is a new species in the Nocardioides genus, the strain can utilize DON toxin as a sole carbon source, and can completely degrade the DON toxin to form CO2 and H2O, completely eliminating the toxic effect, and can also degrade a plurality of fusarium toxins including A-type trichothecene toxin neosolensaric acid (NEO) and B-type trichothecene toxin 3-acetyl-DON (3-Ac-DON), nivalenol (NIV) and fusarenon-X (FUS-X). Therefore, the strain has a wide application prospect in the field of fusarium toxin detoxification.

The present invention relates to a method of converting 3-keto-DON into 3-epi-DON, reducing the content of DON in a composition comprising DON or of reducing the toxicity of a composition comprising DON as well as a method for converting a trichothecene comprising a 3-oxo group into a trichothecene comprising a 3-hydroxy group using one or more polypeptide(s) comprising or consisting of SEQ ID NO. 1 or a sequence having a sequence identity of at least 72.0% to SEQ ID NO. 1. Also envisioned are feed or food additives or feed or food as well as pharmaceutical compositions comprising one or more polypeptide(s) comprising or consisting of a sequence of SEQ ID NO. 1 or a sequence having a sequence identity of at least 72.0% to SEQ ID NO. 1 as well as the manufacture thereof. Encompassed are further polypeptide(s) comprising or consisting of a sequence of SEQ ID NO. 1 or a sequence having a sequence identity of at least 88.5% to SEQ ID NO. 1. Also envisioned are host cells or plants.

A method of reducing the toxicity of a trichothecene mycotoxin such as deoxynivalenol (DON) comprises exposing the trichothecene mycotoxin to a UDP- glycosyltransferase enzyme originating from a strain of Bacillus subtilis. The enzyme catalyzes the conversion of the trichothecene mycotoxin to a non-toxic monoglycosylated metabolite. The enzyme is useful in reducing the toxicity of animal feed or food products which would otherwise be contaminated with the trichothecene mycotoxin.

The present invention relates to methods of modifying trichothecene, mutant variants of polypeptides, additives, methods of manufacture, uses of said polypeptides, polynucleotides, recombinant host cells and polypeptides for treating, ameliorating and / or preventing symptoms caused by fungal poisoning.

The invention belongs to the field of gene engineering, and particularly relates to application of BcGLOA protein in regulation and control of type-B trichothecenes, the BcGLOA protein can reduce accumulation of the type-B trichothecenes in plants, and the accumulation amount of the type-B trichothecenes in crop grains is reduced. Based on detoxification of B-type trichothecene toxins, toxin accumulation in crops is reduced, a specific catalytic mechanism is achieved, and the influence on other non-pathogenic bacteria is small.

Use of an alcohol dehydrogenase of SEQ ID No. 1 containing a metal ion and a quinone cofactor, or a functional variant thereof showing at least 80 %, preferably at least 86 %, especially preferably at least 89 % sequence identity and at least one redox cofactor, for the conversion of at least one trichothecene compound presenting a hydroxyl group at the C-3 atom, as well as methods for the enzymatic conversion of trichothecene compounds and trichothecene conversion additives.