The invention discloses a loop-mediated isothermal amplification (LAMP) primer combination, an LAMP kit and an LAMP method for detecting trichothecenes. The LAMP primer combination consists of a forward outer primer F3, a reverse outer primer B3, a forward inner primer FIP, a reverse inner primer BIP, a forward loop primer LB and a reverse loop primer RB. The detection method disclosed by the invention can be used for simultaneously detecting all trichothecenes, and the detection method is strong in specificity, high in accuracy, simple and convenient to operate, good in practicability and capable of achieving, isothermal amplification; and the detection method is applicable to the high-sensitivity rapid detection of the trichothecenes and is easy for large-scale popularization.