31 results about "Tanshinone I" patented technology

The invention discloses an application of a salvia miltiorrhiza SmPYL4 gene in regulating and controlling the content of tanshinone. The nucleotide sequence of the salvia miltiorrhiza SmPYL4 gene is as shown in SEQ ID NO. 1. Specifically, the expression of the SmPYL4 gene of the salvia miltiorrhiza is silenced or inhibited, so that the content of tanshinone in hairy roots of the salvia miltiorrhiza is increased. The tanshinone is any one or more than two of tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone. The invention further discloses a method for increasing the content of tanshinone in the hairy roots of salvia miltiorrhiza bunge. Researches find that the Salvia miltiorrhiza SmPYL4 gene has a negative regulation effect on synthesis and accumulation of tanshinone components, and the content of the tanshinone components can be effectively increased by silencing the gene. The method has important reference value and significance for cultivation of excellent varieties of salvia miltiorrhiza.

The invention discloses an application of a salvia miltiorrhiza SmPYL8 gene in regulating and controlling the content of tanshinone. The nucleotide sequence of the salvia miltiorrhiza SmPYL8 gene is as shown in SEQ ID NO. 1. Specifically, the expression of the SmPYL8 gene of the Salvia miltiorrhiza is silenced or inhibited, so that the content of tanshinone in hairy roots of the Salvia miltiorrhiza is increased. The tanshinone is any one or more than two of tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone. The invention further discloses a method for increasing the content of tanshinone in the hairy roots of salvia miltiorrhiza bunge. Researches find that the Salvia miltiorrhiza SmPYL8 gene has a negative regulation effect on synthesis and accumulation of tanshinone components, and the content of the tanshinone components can be effectively increased by silencing the gene. The method has important reference value and significance for cultivation of excellent varieties of salvia miltiorrhiza.

The invention discloses an application of a salvia miltiorrhiza SmPP2C24 gene in regulating and controlling the content of tanshinone. The nucleotide sequence of the salvia miltiorrhiza SmPP2C24 gene is as shown in SEQ ID NO. 1. Specifically, the expression of the SmPP2C24 gene of the radix salviae miltiorrhizae is silenced or inhibited, so that the content of tanshinone in hairy roots of the radix salviae miltiorrhizae is increased. The tanshinone is any one or more than two of tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone. The invention further discloses a method for increasing the content of tanshinone in the hairy roots of salvia miltiorrhiza bunge. Researches find that the Salvia miltiorrhiza SmPP2C24 gene has a negative regulation effect on synthesis and accumulation of tanshinone components, and the content of the tanshinone components can be effectively increased by silencing the gene. The method has important reference value and significance for cultivation of excellent varieties of salvia miltiorrhiza.

The present invention discloses a root heterostem mold and its application for improving the growth and effective ingredient content of salvia miltiorrhiza, belonging to the field of microbial technology. The strain is deposited in the China Center for Type Culture Collection on May 31, 2024, and the deposit number is CCTCC NO: M 20241118. The strain obtained by the present invention is root heterostem mold (Paraphoma radicina), which is isolated from the root system of Ammopiptanthus mongolicus, and exhibits excellent ability to dissolve phosphorus, potassium, and secrete indoleacetic acid (IAA). It can effectively promote the growth of salvia miltiorrhiza plants, increase the aboveground biomass and underground biomass of salvia miltiorrhiza plants, and effectively improve the content of effective ingredients in salvia miltiorrhiza, increase the content of tanshinone I, tanshinone II A, cryptotanshinone and salvianolic acid B, and has important application value in the quality improvement cultivation of salvia miltiorrhiza.

The invention discloses a synchronous green extraction method for salvianolic acid B, tanshinone I, cryptotanshinone and tanshinone IIA in salvia miltiorrhiza, which comprises the following steps: crushing the salvia miltiorrhiza into powder, and adding a bio-based solvent into the salvia miltiorrhiza powder for ultrasonic-assisted extraction. According to the synchronous green extraction method of salvianolic acid B, tanshinone I, cryptotanshinone and tanshinone IIA in salvia miltiorrhiza, provided by the embodiment of the invention, when the extraction solvent is glycerol formal / levulinic acid (57.8 / 42.2, v / v), the liquid-solid ratio is 15mL / g, the ultrasonic extraction temperature is 35 DEG C, the ultrasonic extraction time is 30min and the ultrasonic power is 360W, the content of salvianolic acid B, tanshinone I, cryptotanshinone and tanshinone IIA in salvia miltiorrhiza can be effectively reduced, and the extraction efficiency is improved. The extraction rate of salvianolic acid is 85.18 (mg / g), the extraction rate of tanshinone I is 0.347 (mg / g), the extraction rate of tanshinone is 1.120 (mg / g), and the extraction rate of tanshinone IIA is 0.931 (mg / g), which are significantly higher than those of traditional methanol and ethanol. Therefore, according to the method provided by the invention, the salvianolic acid B, the tanshinone I, the cryptotanshinone and the tanshinone IIA are extracted from the radix salviae miltiorrhizae, the efficiency can be effectively improved, the use of organic solvents methanol and ethanol is avoided, and the method is more environment-friendly.

The present invention discloses a method for screening effective substances of traditional Chinese medicine based on a 6-dimensional spider web model, belonging to the field of medical technology. Based on the results of UHPLC analysis, the present invention uses danshensu, chlorogenic acid, procyanidin B2, epicatechin, hyperoside, isoquercetin, rosmarinic acid, lithospermic acid, salvianolic acid B, salvianolic acid A, dihydrotanshinone I, tanshinone I, cryptotanshinone, tanshinone IIA, oleanolic acid, and ursolic acid as the "specific" candidate effective ingredients of the Danshen-Hawthorn medicinal pair. Based on the data of the candidate effective ingredients in six dimensions, namely, effectiveness dimension, compatibility environment dimension, transmission and traceability dimension, content measurability dimension, network pharmacology degree value dimension, and molecular docking target binding activity dimension, a "spider web" model is constructed to obtain the key effective substances of the Danshen-Hawthorn medicinal pair. The present invention provides theoretical support for revealing the compatibility theory of the Danshen-Hawthorn medicinal pair, formulating its quality evaluation standards, and clinical application of the Danshen-Hawthorn medicinal pair.

The invention discloses a salvia miltiorrhiza endogenous oligopeptide composition and application thereof. The composition contains salvia miltiorrhiza endogenous oligopeptides miPEP167b-5p and miPEP171b-1, wherein the concentration ratio of the salvia miltiorrhiza endogenous oligopeptides miPEP167b-5p to the miPEP171b-1 is 0.8-1.2. The oligopeptide used in the composition has high species specificity, does not promote the growth of other plants in the field, and avoids the ecological risk of a traditional broad-spectrum growth regulator. Field experiment results show that after the composition is applied to treatment, the root system yield of salvia miltiorrhiza is increased by 56% compared with that of a control group, the number of roots is increased by 435% compared with that of the control group, the rosmarinic acid content is increased by 43% compared with that of the control group, the salvianolic acid B content is increased by 81%, the tanshinone I content is increased by 214%, the tanshinone IIA content is increased by 75%, the leap-type improvement of the salvia miltiorrhiza medicinal material yield and quality is synchronously achieved, and the application prospect is wide. And reliable technical support is provided for green, efficient and high-quality development of the salvia miltiorrhiza industry.

The invention relates to an application of tanshinone I in preparation of a medicine for treating AIDS (Acquired Immune Deficiency Syndrome), and a tanshinone I compound is used for preparing the medicine for treating the AIDS and is applied to treatment of the AIDS. The Tanshinone I compound can significantly reduce the efficiency of programmed ribosome frameshift when the mRNA of HIV-1 translates a Gag-Pol fusion protein, so that the translation efficiency of the Pol protein is greatly reduced, thereby causing the ratio imbalance of Gag and Pol in cells, causing the virus not to be effectively packaged, and finally causing the sharp reduction of the yield of the virus, and having a good anti-HIV effect.

The invention discloses a method for detecting effective components of a radix salviae miltiorrhizae extracting solution based on a Raman spectrum and a neural network, and belongs to the field of analysis and detection. The detection method comprises the following steps: providing a salvia miltiorrhiza extracting solution sample; acquiring Raman spectrum characteristic data of the extracting solution sample; and inputting the Raman spectrum characteristic data into a quantitative detection model of the effective components of the salvia miltiorrhiza, and determining the concentrations of tanshinone I, tanshinone IIA and salvianolic acid B in the sample to be detected. The method has the advantages of being high in prediction precision (the correlation coefficient R2 reaches 0.98), high in universality and free of destructiveness, and is suitable for rapid quality evaluation of the traditional Chinese medicine compound preparation.

The present invention relates to a quality control method for Jingutongxiao pills, belonging to the technical field of traditional Chinese medicine. The quality control method is based on the existing quality standards, by adding the thin layer identification of licorice, improving the thin layer identification of cyperus rotundus, cinnamon twig, white peony root, cyperus rotundus and gentiana, and using HPLC to simultaneously determine cryptotanshinone, tanshinone I and tanshinone II A The content of the product was optimized and the chromatographic conditions were optimized, thus achieving the technical goal of better controlling the product quality.

The application discloses a tanshinone MAPK4 protein kinase and application of a coding gene thereof in regulating tanshinone content in Salvia miltiorrhiza. The application finds that the tanshinone MAPK4 protein kinase can improve gene transcription level and protein level of a key synthesis enzyme on a tanshinone synthesis path, and then improves tanshinone content (such as tanshinone IIA, dihydrotanshinone I, cryptotanshinone and tanshinone I) in Salvia miltiorrhiza. Therefore, the tanshinone MAPK4 protein kinase, the coding gene and an expression vector containing the coding gene can be used for regulating and improving tanshinone in Salvia miltiorrhiza.

The invention discloses a salvia miltiorrhiza quality evaluation method based on HPLC-DAD and multivariate analysis, and relates to the technical field of medicine quality evaluation, and the salvia miltiorrhiza quality evaluation method comprises the following steps: extracting effective components from salvia miltiorrhiza medicinal material samples collected from different regions by adopting an extraction solvent, the effective components at least comprise salvianic acid A sodium, rosmarinic acid, salvianolic acid B, cryptotanshinone, tanshinone I and tanshinone IIA, HPLC-DAD is adopted for chromatographic analysis, and the extracted effective components are subjected to multivariate statistical analysis. According to the system, the quality difference of salvia miltiorrhiza from different producing areas is evaluated, and scientific basis and data support are provided for standardized planting, producing area screening and clinical reasonable application of salvia miltiorrhiza medicinal materials.

The present invention discloses a long-branch Trichoderma strain and a freeze-dried powder thereof for enhancing the fat-soluble medicinal components of Salvia miltiorrhiza, and belongs to the field of microbial technology. The strain is named SBa-TL, and is deposited in the China Center for Type Culture Collection on April 18, 2025, with a deposit number of CCTCC NO: M 2025821. The present invention provides a long-branch Trichoderma strain and a long-branch Trichoderma freeze-dried powder that can enhance the fat-soluble medicinal components of Salvia miltiorrhiza. The strain and the freeze-dried powder prepared therefrom can effectively promote the growth of Salvia miltiorrhiza plants, increase the above-ground and underground biomass of Salvia miltiorrhiza plants, and significantly improve the content of medicinal components in Salvia miltiorrhiza, especially increase the content of tanshinone I, tanshinone IIA and cryptotanshinone. In the cultivation of improving the quality of Salvia miltiorrhiza, the strain has significant application value.

A traditional Chinese drug monomer composition for treating Helicobacter pylori and its preparation method and use thereof, the steps are: a. dissolving drugs: dissolving berberine, evolitrine, and tanshinone I respectively in absolute ethanol; b. combining the drugs: mixing an ethanol solution obtained from the step a according to the mass ratio of berberine, evolitrine, and tanshinone I at 6:1:(0.75-6) to obtain the composition. The present disclosure successfully prepares a traditional Chinese drug monomer composition for the treatment of Helicobacter pylori, which has an extremely obvious inhibitory effect on Helicobacter pylori both in vivo and in vitro, and has a specific effect, low toxicity, and is not easy to produce drug resistance.

The application discloses a kind of heart tonic tablet fingerprint spectrum detection methods, belong to traditional Chinese medicine detection technical field.The technical scheme includes: to system applicability solution, reference solution, medicinal material solution, test solution are detected by high performance liquid chromatography, obtain chromatogram;According to the chromatogram obtained in S5, determine the chemical composition of each chromatographic peak in the fingerprint spectrum, respectively 3 peaks of calycosin-7-glucoside, 4 peaks of 4-methoxy salicylaldehyde, 6 peaks of salvianolic acid B, 9 peaks of tanshinone I, 10 peaks of cryptotanshinone, 12 peaks of tanshinone IIA;According to the chromatogram obtained in S5, determine the source of each chromatographic peak in the fingerprint spectrum.The application is applied to heart tonic tablet detection aspect, solves the determination of only tanshinone IIA under the content item of existing heart tonic tablet current quality standard, control index is single, thus cannot comprehensively reflect the overall quality level of preparation problem, with the characteristics of accurate, stable and reliable detection result.

The invention discloses a method for determining the content of five components in a white jade ointment based on quantitative analysis of multi-components by single marker, and relates to the technical field of medicine detection. According to the method, tanshinone IIA is used as an internal standard substance for quantitative analysis of multi-components by single marker, relative correction factors of 1, 4-di [4-(glucose oxygen) benzyl]-2-isobutyl malate, tanshinone I, 6-gingerol and beta, beta '-dimethylacryloyl alkannin are established, and the content of each component in the white jade ointment is calculated through the relative correction factors. The method comprises the following specific steps: preparing a test solution; preparing a reference substance solution; performing chromatographic condition and system adaptability test; and measuring and calculating. According to the method, the oneness and one-sidedness of quality control of the white jade ointment are overcome, and the method has important significance in further improving the quality control level of the white jade ointment, ensuring the clinical curative effect and improving a related evaluation system.

Disclosed is compound YM155, tanshinone I or cryptotanshinone, or a pharmaceutically acceptable salt thereof, a solvate thereof, a solvate of the pharmaceutically acceptable salt thereof, or a crystal form thereof, for treating and / or preventing diseases caused by coronavirus.

The invention discloses application of Salvia miltiorrhiza MAPK4 protein kinase and a coding gene thereof in regulating the content of tanshinone compounds in Salvia miltiorrhiza. It is found that the salvia miltiorrhiza MAPK4 protein kinase can improve the gene transcription level and the protein level of key synthetase on a tanshinone substance synthesis pathway, and then the content of tanshinone substances (such as tanshinone IIA, dihydrotanshinone I, cryptotanshinone and tanshinone I) in salvia miltiorrhiza is increased. Therefore, the Salvia miltiorrhiza MAPK4 protein kinase, the coding gene thereof and the expression vector containing the coding gene can be used for regulating and improving tanshinone substances in Salvia miltiorrhiza.

The application discloses a synchronous green extraction method of salvianolic acid B, tanshinone I, cryptotanshinone and tanshinone IIA in Danshen, which comprises the following steps: crushing Danshen into powder, and adding a bio-based solvent to the Danshen powder for ultrasonic-assisted extraction. When the extraction solvent is glycerol formal / acetic propionic acid (57.8 / 42.2, v / v), the liquid-solid ratio is 15 mL / g, the ultrasonic extraction temperature is 35 DEG C, the ultrasonic extraction time is 30 min, and the ultrasonic power is 360 W, the extraction rate of salvianolic acid B, tanshinone I, cryptotanshinone and tanshinone IIA in Danshen is 85.18 (mg / g), 0.347 (mg / g), 1.120 (mg / g) and 0.931 (mg / g) respectively, which is significantly higher than that of traditional methanol and ethanol. Therefore, the method provided by the application can effectively improve the extraction efficiency of salvianolic acid B, tanshinone I, cryptotanshinone and tanshinone IIA in Danshen, avoid the use of organic solvents such as methanol and ethanol, and is more green and environment-friendly.

This invention relates to an additive for NK cell induction and expansion culture, a culture medium, and their applications. The additive comprises tanshinone I and berberine. Specifically, PBMCs are isolated from human peripheral blood. On the third day of NK cell activation culture, tanshinone I (final concentration 0.1-10 µM) and berberine (final concentration 10-100 µM) are added to the culture system. Both tanshinone I and berberine are natural products with wide availability. Their combined use can significantly increase the proportion of memory NK cells, while exhibiting high viability, high purity, high expansion rate, and high cytotoxic activity. Flow cytometry analysis showed a significant increase in the positive expression rates of some NK cell markers, including CD62L, CD127, and Eomes.

The application discloses a method for determining the content of five components in Baiyu ointment based on a quantitative analysis of multiple components, and relates to the technical field of drug detection. ′ The method uses tanshinone II A as an internal standard for quantitative analysis of multiple components, establishes the relative correction factors of 1,4-di[4-(glucose oxygen) benzyl]-2-isobutyl malonic acid ester, tanshinone I, 6-gingerol, beta, beta The content of each component in the Baiyu ointment is calculated through the relative correction factors, and the specific steps include: preparation of a test sample solution; preparation of a reference sample solution; chromatographic conditions and system suitability test; determination and calculation. The application overcomes the single and one-sided quality control of the Baiyu ointment, and has important significance for further improving the quality control level of the Baiyu ointment, guaranteeing the clinical curative effect and improving the related evaluation system.

The invention discloses a tanshinone I intrauterine sustained-release medicament for treating gynecological tumors and application thereof, which is characterized in that tanshinone I is prepared into a nano-structure lipid carrier with the average particle size of 100-200nm, so that the water solubility and biological membrane permeability of the nano-structure lipid carrier are remarkably improved; and then the carrier is loaded in a biodegradable polymer slow-release rod which can be placed in a uterine cavity. The sustained-release rod can release drugs when the environment in the uterine cavity declines, and local, long-acting and targeted drug delivery to the focus part is achieved. An in-vitro cell experiment proves that the inhibiting effect of the preparation on hysteromyoma cells is obviously higher than the toxicity of the preparation on normal hysteromyoma cells, and the selectivity index is greater than 2; therefore, the problems of poor solubility of tanshinone I and weak systemic administration targeting are solved, and the tanshinone I has the outstanding advantages of high efficiency, low toxicity and good patient compliance.

The application discloses application of a Salvia miltiorrhiza SmPYL8 gene in regulating tanshinone content, and a nucleotide sequence of the Salvia miltiorrhiza SmPYL8 gene is shown as SEQ ID NO. 1. Specifically, the expression of the Salvia miltiorrhiza SmPYL8 gene is silenced or inhibited, so that the content of tanshinone in the hairy roots of Salvia miltiorrhiza is increased. The tanshinone is any one or two or more of tanshinone I, dihydrotanshinone I, tanshinone II A and cryptotanshinone. The application further discloses a method for increasing the content of tanshinone in the hairy roots of Salvia miltiorrhiza. It is found by the application that the Salvia miltiorrhiza SmPYL8 gene has a negative regulation effect on the synthesis and accumulation of tanshinone components, and silencing the gene can effectively increase the content of tanshinone components. The application has important reference value and significance for the cultivation of excellent varieties of Salvia miltiorrhiza.

The invention discloses a trichoderma longibrachiatum strain for enhancing fat-soluble medicinal components of salvia miltiorrhiza and application of freeze-dried powder of the trichoderma longibrachiatum strain, and belongs to the technical field of microorganisms. The strain is named as SBa-TL and is preserved in the China Center for Type Culture Collection, the preservation time is April 18, 2025, and the preservation number is CCTCC NO: M 2025821. The invention provides a trichoderma longibrachiatum strain and trichoderma longibrachiatum freeze-dried powder capable of enhancing fat-soluble medicinal components of salvia miltiorrhiza, and the strain and the prepared freeze-dried powder can effectively promote the growth of salvia miltiorrhiza plants, increase the overground and underground biomass of the salvia miltiorrhiza plants, remarkably improve the content of medicinal components in the salvia miltiorrhiza, and improve the yield of the salvia miltiorrhiza. The Salvia miltiorrhiza strain can improve the content of Salvia miltiorrhiza, especially the content of tanshinone I, tanshinone IIA and cryptotanshinone, and has significant application value in cultivation for improving the quality of Salvia miltiorrhiza.

The invention provides a producing area processing method for improving the content of index components of salvia miltiorrhiza, and belongs to the technical field of traditional Chinese medicine processing. The invention provides a production place processing method of salvia miltiorrhiza. The production place processing method comprises the following steps: performing adversity stress treatment on salvia miltiorrhiza; and drying the salvia miltiorrhiza subjected to adversity stress treatment. When the processing method of the radix salviae miltiorrhizae in the producing area is used for processing the fresh radix salviae miltiorrhizae medicinal material, the content of four index components including salvianolic acid B, cryptotanshinone, tanshinone I and tanshinone IIA in the radix salviae miltiorrhizae is remarkably increased, and further, the processing method of the radix salviae miltiorrhizae in the producing area has the effects of promoting the increase of secondary metabolites of the radix salviae miltiorrhizae and improving the yield of the radix salviae miltiorrhizae. The content of index components of salvia miltiorrhiza is increased, and meanwhile, the obtained dried salvia miltiorrhiza medicinal material is good in appearance property and good in condition. In conclusion, the radix salviae miltiorrhizae producing area processing method provided by the invention is low in cost, simple and feasible to operate in an adversity stress stage and a drying stage after stress, low in manpower and material resource consumption, environment-friendly, wide in application prospect and good in economic value.

Compound YM155, tanshinone I or cryptotanshinone, or a pharmaceutically acceptable salt thereof, a solvate thereof, a solvate of a pharmaceutically acceptable salt thereof, or a crystal form thereof, is used for treating and / or preventing diseases caused by coronaviruses.

The application discloses application of a Salvia miltiorrhiza SmPYL4 gene in regulating tanshinone content, and a nucleotide sequence of the Salvia miltiorrhiza SmPYL4 gene is shown as SEQ ID NO. 1. Specifically, the expression of the Salvia miltiorrhiza SmPYL4 gene is silenced or inhibited, so that the content of tanshinone in the hairy roots of Salvia miltiorrhiza is increased. The tanshinone is any one or two or more of tanshinone I, dihydrotanshinone I, tanshinone II A and cryptotanshinone. The application further discloses a method for increasing the content of tanshinone in the hairy roots of Salvia miltiorrhiza. The application finds that the Salvia miltiorrhiza SmPYL4 gene has a negative regulation effect on the synthesis and accumulation of tanshinone components, and silencing the gene can effectively increase the content of tanshinone components. The application has important reference value and significance for the cultivation of excellent varieties of Salvia miltiorrhiza.

The invention discloses a fingerprint detection method for tonifying and heart-strengthening tablets, and belongs to the technical field of traditional Chinese medicine detection. According to the technical scheme, the method comprises the following steps: performing high performance liquid chromatography detection on a system applicability solution, a reference substance solution, a medicinal material solution and a test solution to obtain chromatograms; s5, determining the chemical components of each chromatographic peak in the fingerprint spectrum according to the chromatogram obtained in S5, namely calycosin glucoside at the peak 3, 4-methoxysalicylaldehyde at the peak 4, salvianolic acid B at the peak 6, tanshinone I at the peak 9, cryptotanshinone at the peak 10 and tanshinone IIA at the peak 12; and determining the source of each chromatographic peak in the fingerprint spectrum according to the chromatogram obtained in the step S5. The method is applied to the detection of the tonifying and heart-strengthening tablets, solves the problem that the whole quality level of the preparation cannot be comprehensively reflected because only tanshinone IIA is determined and the control index is single under the content item of the current quality standard of the existing tonifying and heart-strengthening tablets, and has the characteristics of accurate detection result, stability and reliability.

The invention discloses a traditional Chinese medicine efficacy substance screening method based on a 6-dimensional cobweb model, and belongs to the technical field of medicine. Based on a UHPLC (Ultra High Performance Liquid Chromatography) analysis result, tanshinol, chlorogenic acid, procyanidine B2, epicatechin, hyperoside, isoquercitrin, rosmarinic acid, alkannic acid, salvianolic acid B, salvianolic acid A, dihydrotanshinone I, tanshinone I, cryptotanshinone, tanshinone IIA, oleanolic acid and ursolic acid are used as specific candidate functional components of the salvia miltiorrhiza-hawthorn medicine pair; and constructing a cobweb model based on data of the candidate functional components in six dimensions, namely an effectiveness dimension, a compatibility environment dimension, a transmission and traceability dimension, a content testability dimension, a network pharmacology value dimension and a molecular docking target binding activity dimension, so as to obtain the key medicinal effect substances of the salvia miltiorrhiza-hawthorn drug pair. The invention provides theoretical support for revealing the compatibility theory of the salvia miltiorrhiza-hawthorn medicine pair, formulating the quality evaluation standard and clinical application of the salvia miltiorrhiza-hawthorn medicine pair.

The invention discloses application of a salvia miltiorrhiza bHLH128 transcription factor and a coding gene thereof in regulating and controlling the content of tanshinone compounds in salvia miltiorrhiza. It is found that the salvia miltiorrhiza bHLH128 transcription factor can be combined with a promoter of a gene CPS1, the level of key enzyme CPS1 on a tanshinone substance synthesis pathway is increased, and then the content of tanshinone substances (such as tanshinone IIA, dihydrotanshinone I, cryptotanshinone and tanshinone I) in salvia miltiorrhiza is increased. Therefore, the salvia miltiorrhiza bHLH128 transcription factor, the coding gene of the salvia miltiorrhiza bHLH128 transcription factor and the expression vector containing the coding gene can be used for regulating and improving tanshinone substances in salvia miltiorrhiza.