92 results about "Tanshinone I" patented technology

The invention discloses a coding gene sequence of an AP2 / ERF transcription factor SmAP2 / ERF82 for regulating tanshinone synthesis. The SmAP2 / ERF82 gene provided by the invention has a nucleotide sequence as shown in the SEQ ID No.1. The gene encoded protein has an amino acid sequence as shown in the SEQ ID No.2. A SmAP2 / ERF82-RNAi vector and a SmAP2 / ERF82-overexpression vector are constructed in the invention. Through a genetic transformation method for transformation of red sage root, transgenic hairy roots are obtained. In comparison with a control line, contents of dihydrotanshinone I, cryptotanshinone, tanshinone I and tanshinone II A in the SmAP2 / ERF82-RNAi line are remarkably reduced, and contents of dihydrotanshinone I and cryptotanshinone in the SmAP2 / ERF82-overexpression line areremarkably raised. The SmAP2 / ERF82 provided by the invention has the function of positive regulation of tanshinone compound biosynthesis. The compounds have remarkable efficacy of treating cardiovascular diseases. The invention brings forth new ideas for the research on molecular mechanism for tanshinone synthesis regulation and lays a foundation for the biological research on synthesis of tanshinone.

The invention discloses a method for extracting and preparing tanshinone compounds from dregs of radix salviae miltiorrhizae by virtue of a saccharification pretreatment method. The method comprises the steps of firstly carrying out saccharification treatment on waste dregs of radix salviae miltiorrhizae to damage cell wall cellulose, hemicelluloses and lignocelluloses, extracting treatment liquid by virtue of an organic solvent, concentrating extraction liquid to a certain volume, carrying out ethanol elution on a macroporous resin column, collecting optimized eluate parts, concentrating, and carrying out spray drying, so as to prepare chemical components of tanshinone. According to the method, the extraction rate can reach 80%-100%, and the purity can reach 70%-95%; the content of tanshinone IIA can reach 36%, the content of tanshinone I can reach 32%, the content of cryptotanshinone can reach 8%, the content of dihydrotanshinone can reach 9%, and the content of active ingredients is high. Experiment results prove that the tanshinone compounds have well efficacies of promoting blood circulation and removing stasis and can be used for preparing medicines or functional health products capable of preventing and treating cardiovascular and cerebrovascular diseases.

The invention is a high performance liquidoid analytical method of tanshinone IIA sodium sulfonate which employs the reverse-phase gradient elution method, The proportion of organic phase and aqueous phase is 0-80:20-40 at 0 minute -8 to 20 minutes,the proportion of organic phase and aqueous phase is 80-95:5-20 at 8 minutes to 20 minutes -20 to 40 minutes. The invention can detect the tanshinone IIA, tanshinone I sodium sulfonate and other relative substance in tanshinone IIA sodium sulfonate at the same time, resolve the shortage of general liquid phase analysis, effectively control the quality of raw material of tanshinone IIA sodium sulfonate and its preparation, quickly and conveniently detect the relative substance of raw material of tanshinone IIA sodium sulfonate and its preparation.

The invention belongs to the field of preparations, and particularly relates to radix salviae miltiorrhizae extract nanocrystalline self-stabilization Pickering emulsion and preparation method thereof. According to the radix salviae miltiorrhizae extract nanocrystalline self-stabilization Pickering emulsion disclosed by the invention, a radix salviae miltiorrhizae extract of which the salvianolicacid B content is 60-100mg / g, the tanshinol content is 3-10mg / g, the rosmarinic acid content is 3-10mg / g, the tanshinone II A content is 5-20mg / g, the cryptotanshinone content is 2-10mg / g and the tanshinone I content is 3-10mg / g is used as a raw material medicine, and the radix salviae miltiorrhizae extract, an oil phase and an aqueous phase are prepared into oil-in-water Pickering emulsion. The nanocrystalline self-stabilization Pickering emulsion having stability properties is prepared through using the radix salviae miltiorrhizae extract containing water-soluble medicine effect ingredientsand insoluble medicine effect ingredients of radix salviae miltiorrhizae at the same time as the raw material medicine, wherein the water-soluble ingredients of salvianolic acid B and the like are distributed in the aqueous phase, and insoluble ingredients of tanshinone and the like are partially distributed in the oil phase and are partially adsorbed on an emulsion droplet interface, so that theemulsion is stabilized.

The invention relates to the field of natural medicine, in particular to a use of ethyl acetate extract of Salvia miltiorrhiza, namely tanshinone, in the preparation of drugs to resist uric acid nephropathy, and a use thereof in the preparation of antioxidant drugs. The drugs containing tanshinone are applied to treat uric acid nephropathy, particularly to prevent or treat hyperuricemia, gout andhyperuricemia-induced chronic nephropathy. Tanshinone mainly includes diterpene phenanthraquinone ingredients such as tanshinone IIA, 15,16-dihydrotanshinone I, cryptotanshinone and miltirone; owing to their synergy, the ingredients have better therapeutic effect in terms of prevention and treatment of uric acid nephropathy than single tanshinone IIA and the clinically common drug allopurinol under single dosage. When the four ingredients, tanshinone IIA, 15,16-dihydrotanshinone I, cryptotanshinone and miltirone, are combined in the optimal ratio of 6:3:6:4 into a tanshinone mixture, best prevention and treatment effect is provided for mouse uric acid nephropathy; through the use of the tanshinone combinations, Kunming mice co-modeled with adenine and oteracil potassium are significantly lower than model groups in terms of blood uric acid, creatinine and urea nitrogen, and kidney protection is achieved.

The invention is concerned with a kind of extract of total ketone of salviae miltiorrhizae and total phenolic acid and its produce method form radix Salviae Miltiorrhizae. The extract of total ketoneof salviae miltiorrhizae has cryptotanshinone, tanshinone I, tanshinone IIA, methyl Tanshinon, dihydrotanshinon I and ramification. The extract of total phenolic acid has salvianolic acid A, salvianolic acid B, protocatechuic aldehyde and ramification. The extract can be got form one or arbitrary compound of extraction with solvent method, macroporous resin method, column chromatography and liquid-liquid counter-current chromatography. The summation of the content to each total ketone of salviae miltiorrhizae is 20 to 100 percnte (w / w) of the extract of total ketone of salviae miltiorrhizae, the contene of cryptotanshinone, tanshinone I and tanshinone IIA is 5 to 100 percent (w / w) of whole content of total ketone of salviae miltiorrhizae. The summation of the content to each total phenolicacid is 5 to 100 percent (w / w) of the extract of the radix salviae miltiorrhizae total phenolic acid. The content of salvianolic acid B is the 5 to 100 percent (w / w) of the whole salvianolic acid.