41 results about "Ammopiptanthus mongolicus" patented technology

The invention discloses a CBF transcription factor capable of regulating plant stress resistance, and a coded gene and application thereof. A CBF full-length gene is cloned and identified from Ammopiptanthus Mongolicus by an RACE strategy; a semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) test proves that the gene is induced by low temperature to start to express in the first hour after cold treatment and have the highest expression quantity after two hours. According to bioinformatics analysis, AmCBF gene coded protein has an AP2 structure domain and a conservative characteristic polypeptide sequence in a CBF family and belongs to a CBF gene family in an AP2/EREBP transcription factor family. Compared with wild type tobaccos, the AmCBF gene-transcribed tobacco leaves after cold treatment obviously reduce percolation ratio of electrolyte and obviously increase proline content and soluble sugar content, so the cold resistance is improved. A drought tolerance test shows that the drought tolerance of the transgenic tobaccos is greatly improved. The test result shows that the AmCBF can respond adversity signals and can play a role.

The invention discloses an in-vitro rapid propagation method of rare and endangered plant ammopiptanthus mongolicus, belongs to the biological field and aims to solve the problems of severe browning and differentiation difficulty in callus induction in existing methods. The in-vitro rapid propagation method of the ammopiptanthus mongolicus comprises steps of obtaining of ammopiptanthus mongolicus explants, induction culture of cluster buds, extension culture of the cluster buds, rooting culture and expansion propagation. Compared with existing in-vitro culture methods of the ammopiptanthus mongolicus, the method has the advantages that cotyledonary nodes are directly subjected to induction of cluster buds and rooting is performed, callus cannot be produced in the process, thus, the step of callus induction is omitted, the cotyledonary nodes are directly cultured into adventitious buds, the in-vitro culture time is greatly shortened, and the whole process from seed germination, obtaining of cotyledonary nodes, induction and extension of the cluster buds to rooting only takes 79 d.

The invention provides a propagation method of ammopiptanthus mongolicus tissue culture seedlings. The method includes the steps: 1) sequentially flushing ammopiptanthus mongolicus seeds by the aid ofrunning water, soaking and disinfecting the flushed seeds by the aid of ethyl alcohol, soaking and disinfecting the seeds by the aid of HgCl2 solution, and flushing the seeds by the aid of sterile water to obtain disinfected seeds; 2) inoculating the disinfected seeds into 1/2MS solid media to culture the seeds for 10-20 days to obtain aseptic seedlings; 3) acquiring stem sections with knobs fromthe aseptic seedlings, and inoculating the stem sections into axillary bud induction media to culture the stem sections for 15-20 days to obtain axillary buds; 4) inoculating the axillary buds obtained in the steps 3) into rooting media to culture the axillary buds for 10-15 days to obtain the tissue culture seedlings. According to the method, explants are acquired from aseptic seedlings, so thatpropagation of the tissue culture seedlings is performed, the method is simple to operate, short in use time, zero in contamination rate and high in emergence rate.

The invention discloses stress tolerance related transcription factor protein NAC and application thereof, wherein the amino acid sequence is shown by SEQ ID No.1. According to the invention, a transcription regulation factor NAC protein is cloned from extreme drought tolerant plant ammopiptanthus mongolicus, and a plant expression vector is established and introduced into arabidopsis through agrobacterium-mediated transformation; and through the means such as preliminary screening of hygromycin, molecular detection, drought stress and in-vivo iron content measurement, a transgenic arabidopsis strain with remarkably improved drought resistance and iron absorbability is obtained. A new variety of transgenic plant capable of efficiently absorbing iron and resisting drought is obtained, which is of great significance in improving the crop quality and enhancing the plant tolerance to adversity stress.

The invention discloses an ammopiptanthus mongolicus cold-resistant gene AmEBP1, and relates to a national secondary endangered and protected evergreen broad-leaved woody plant derived from the tertiary period in the desert regions of northwest China. The molecular cloning of the gene comprises the following steps of: obtaining a low temperature-induced and up regulation-expressed EST fragment through low temperature induction and differential expression analysis; and obtaining a full-length cDNA sequence of the gene through RACE amplification, and registering in a gene bank with the registration number of DQ519359. The AmEBP1 contains a whole ORF region with 1,188 nucleotides at the overall length, and the nucleotides code 395 amino acids and a terminator codon (TGA); a eukaryotic expression vector pCAMBIA2300-AmEBR1 is used to transform arabidopsis thaliana, and the obtained transgenic plant has improved cold resistance; and the ammopiptanthus mongolicus cold-resistant gene AmEBP1 is the first international woody plant cold-resistant gene which is cloned and passes genetic transformation verification.

The invention discloses a subsequence of Ammopiptanthus mongolicus PeNAC1 gene 1217bp promoter, which is named as PeNAC1P. A PLACE database can be used for analyzing and predicting cis acting elements which are contained in PeNAC1P and are relevant to the induction of drought, high salts, ABA, GA, JA, SA, MeJA and other hormones, elements relevant to bacterial and salts, cis elements responding to calcium ions and cis elements participating in the sugar signal transfer. Analyzed by a transient expression experiment, the activity of the PeNAC1P promoter is induced by the salts, GA and IAA. The Ammopiptanthus mongolicus PeNAC1P promoter provided by the research belongs to an induction type promoter and provides an alternative promoter for generating a normal tolerant transgene plant on the basis of researching the function.

The invention discloses a stumping and rejuvenating method of natural ammopiptanthus mongolicus which belongs to rare or endangered species. An optimized implement way of the stumping and rejuvenating method of the ammopiptanthus mongolicus includes the following steps that at the end of winter and the beginning of spring, ammopiptanthus mongolicus populations are divided to be in stagger arrangement; vegetation coverage of a stumping area is more than 60 %; an area ratio of the stumping area and a reserved area is 1:2; the band width of the stumping area is 2 m; the band width of the reserved area is 4 m; a whole cluster is cut through sharp tools; the height of stubble is 0-3 cm apart from the ground; and the diameter class thickness of stump plants is 15.09-19.95 mm. The stumping and rejuvenating method of the ammopiptanthus mongolicus has the advantages of solving the problems that the ammopiptanthus mongolicus is easy to have problems of growth recession, dried-up to death and being endangered due to harsh environment and increasing ages, being simple in operating step, easy to popularize and capable of promoting growth of sprout tillers and new branches on root portions, promoting spontaneous recovery of the ammopiptanthus mongolicus, eliminating plant diseases and insect pests, greatly improving blossom and maturing rates due to the fact that the stump plant starts to bloom and seed at the second year and being capable of feasibly protecting the ammopiptanthus mongolicus which belongs to the rare or endangered species.

The invention relates to a plant water-saving antitranspirant and a preparation method thereof, and belongs to the technical field of plant water-saving agents. The plant water-saving antitranspirantis capable of using a wax layer of ammopiptanthus mongolicus skin to combine with n-dodecyl alcohol, n-hexadecyl alcohol and aromatic alcohol, and is sprayed on the leaf surface to form a layer of a transparent molecular film, so the mass transfer resistance of water gas-liquid transmission is increased, the antitranspirant achieves the effect of a vapor exchange energy barrier and has the stronginhibition and water retaining effect, the antitranspirant can be rapidly and autonomously spread out to form the molecular film after sprayed on the leaf surface, and a leaf surface water evaporationrate can be effectively delayed. The plant water-saving antitranspirant is capable of using drought-resisting stress-resisting natural substances, such as more than ten types of amino acids and alkaloid contained in the ammopiptanthus mongolicus, to combine with a drought-resisting element, keeping and improving the plant cellular affinity and water retaining capacity, preventing the cell dehydration, enabling plant cells to have the apparent drought-resisting capacity, reducing the plant water transpiration, remarkably increasing the chlorophyll content, improving the photosynthesis, enhancing the plant enzyme system physiological activity, and stimulating the growth of a plant root system.

The invention provides a tissue culture method of ammopiptanthus mongolicus. Embryos in ammopiptanthus mongolicus seeds are taken as explants, through primary inducing culture, subculture, rooting culture and transplantation, selection of ammopiptanthus mongolicus explants, the optimal culture condition, optimal culture mediums at different stages, and the compounding ratio of cultivation substrates are systematically researched. Through experiments, the optimal culture mediums at different stages and the combination of the culture mediums in tissue culture of the ammopiptanthus mongolicus areobtained, through the optimal culture condition and the culture manner are obtained, the browning problem in the tissue culture process of the ammopiptanthus mongolicus is solved, the survival rate of tissue culture seedlings is increased, the production cost is reduced, and a reliable base is established for realizing the industrialized production of the ammopiptanthus mongolicus. The tissue culture method disclosed by the invention is simple and convenient to operate and low in cost and has favorable application prospects.

The invention belongs to the technical field of forestry seedling raising, and particularly relates to a container seedling raising method for ammopiptanthus mongolicus, which comprises the following steps that: S1, sandy soil, nursery soil and a compound fertilizer are mixed to prepare a seedling raising matrix according to a mass ratio of 2: (0.9-1.1): (0.9-1.1); S2, a seedling raising bag is filled with the seedling raising matrix, the seedling raising bag is placed in a seedling raising pond, and the seedling raising matrix and the seedling raising bag are thoroughly watered; S3, the seeds are disinfected and washed, the seeds are soaked in water for 1-1.2 h, water is added to submerge the seeds by 1-1.3 cm after washing and draining, the seeds are covered with water absorption cloth, and soaking lasts for 10-15 h; S4, sowing is performed when the temperature of the seedling raising matrix is above 15 DEG C, and the seedling raising bag is covered with a plastic film after sowing; S5, after the seedlings break through the ground by 1-1.5 cm, the plastic film is uncovered, watering is conducted once when the first pair of real leaves grow, weeds are removed at the same time, and then watering is conducted once every 15-20 days; S6, after the fourth pair of real leaves and more than three branches of the seedlings grow, and the height of the seedlings is more than 15 cm, outplanting and transplanting are performed. The container seedling raising method for ammopiptanthus mongolicus can prevent ammopiptanthus mongolicus seedlings from the disease, and meanwhile, the germination rate, the survival rate and the outplanting rate of the seedlings are remarkably improved.

The invention discloses a container seedling raising method of ammopiptanthus and relates to the technical field of planting. The method comprises the steps that during seedling raising of ammopiptanthus, a seedling raising medium keeps insulated from external soil. It is discovered through a large amount of creative labor of an inventor that ammopiptanthus belongs to the tap root system, if during seedling raising, a container is in contact with the soil, the root systems of ammopiptanthus seedlings can quickly penetrate through the container and enter the soil, so that the root systems of the ammopiptanthus seedlings are easily injured during transplantation, and the content above is the main reason of low survival rate of the ammopiptanthus seedlings after transplantation. In this way,through the inventor, the seedling raising medium is insulated from the soil, the root systems of the ammopiptanthus seedlings always grow in the container, the integrality of the root systems of theammopiptanthus seedlings can be maintained during transplantation, and the survival rate of transplantation is greatly increased. According to the planting method of ammopiptanthus, the container seedling raising method of ammopiptanthus is adopted for seedling raising, and cultivated seedlings continue to be cultivated and ripen. The planting method is high in survival rate, and large-scale planting of ammopiptanthus can be achieved.

The invention relates to a rapid propagation method for Ammopiptanthus mongolicus, belonging to the technical field of vegetative propagation. The rapid propagation method provided by the invention solves the problem of difficult rooting in cutting propagation of the Ammopiptanthus mongolicus on the basis of maintaining excellent characters of a valuble endangered plant namely the Ammopiptanthus mongolicus through arrangement of conditions of callus induction, differentiation culture and rooting culture for aseptic seedlings of the Ammopiptanthus mongolicus. The rapid propagation method has the advantages of short propagation period, rapid rooting rate, high survival rate and low culture cost, and can lay a good preliminary foundation for protection of Ammopiptanthus mongolicus germplasm resources and production and research of high-quality Ammopiptanthus mongolicus seedlings.

The invention discloses a seedling culture afforestation method of ammopiptanthus mongolicus in desert regions and belongs to the technical field of seedling culture afforestation of ammopiptanthus mongolicus. The method mainly includes: improving soil; arranging grass check sand barriers; culturing seedlings for afforestation; performing later-period management. By adopting the method, seedling culture survival rate can reach higher than 85%, and survival rate of artificial planting of ammopiptanthus mongolicus is increased effectively. A soil improver can improve obstacle soil, utilization rate of chemical fertilizer is increased, root systems are promoted to be developed, and a biomass material has obvious effect in prevention and control of soil disease microorganisms. A grass check fixing method has good sand prevention effect, can promote growth of ammopiptanthus mongolicus and can effectively increase speed of returning sand into soil. Biochemical fertilizer utilizes organic matter as a raw material, so that the raw material is rich in nutrient and low in cost.

The invention discloses a method for producing seeds of ammopiptanthus nanus for ex-situ conservation in a desert region. The flower bud differentiation stage of ammopiptanthus nanus is managed. The method comprises the specific steps of fertilization, irrigation, prevention and control of harmful creatures and overwintering management and protection; flowering period management of ammopiptanthusnanus comprises the specific steps that prevention of low-temperature freezing damage, prevention of high wind, fertilization in spring, irrigation and artificial supplementary pollination; fruiting period management of ammopiptanthus nanus comprises the specific steps of topdressing, irrigation, prevention and control of harmful creatures and seed harvesting; through artificial fine breeding andmanagement, ammopiptanthus nanus can bloom in quantity, lots of seeds with vitality can be produced, the difficult problem of dysgenesis in ex-situ conservation is solved, and the method has importantsignificance on germplasm resource conservation and population enlargement and reproduction of a rare and endangered plant ammopiptanthus nanus.

The present invention relates to a new anti-cancer medicine. It includes extract of seed, elaf and stem of saline-alkali land desert plant ammopiptanthus mongolicus-ammopiptanthus mongolicus lipid. The invention also relates to the application of ammopiptanthus mongolicus and ammopiptanthus mongolicus lipid in preparation of anti-cancer Chinese medicine preparation, oral preparation and non-oral preparation. The clinical test shows that it has obvious effect for inhibiting liver cancer, its toxicity is low, it has the action of stopping pain, can prolong life and has the action of regulating lipometabolism.

The invention discloses bacteriostatic liquid. The bacteriostatic liquid is prepared from a nourishing solution and repair blended essence; the nourishing solution is prepared from the following materials: water, radix oenotherae erythrosepalae, rose flowers, honeysuckle flowers, frankincense, cortex cinnamomi, silk fibroin and folium artemisiae argyi; the repair blended essence is prepared from the following materials: water, fructus canarii albi, aged black tea leaves, saffron crocus stigma, yellow old ginger, sandalwood, sophora japonica, lemons, ammopiptanthus mongolicus, licorice roots, myrrh, cloves, methyl salicylate, turpentine and silk glutin. The bacteriostatic liquid has the advantages that pelvic gaps are relieved, and external virus infection of the pelvic environment is reduced; menstrual disorder, dysmenorrhea, leucorrhea abnormality, uterus coldness and sexual apathy are alleviated; the sensitivity of the couple's sexual life is improved, and the internal and external vagina contours are enabled to be compact, lubricated, delicate and pink; the two-way balance of the human body is regulated, and the body's immunity is enhanced; internally deposited toxin is removed,the ovarian self-purification ability is recovered, and the skin tone is improved.

The invention relates to the technical field of gene engineering, in particular to an Ammopiptanthus mongolicus (Maxim. ex kon) Chengf. hairy root system induction and culture method for improving the yield of formononetin. The Ammopiptanthus mongolicus (Maxim. ex kon) Chengf. hairy root system induction and culture method comprises the following steps of (1) obtaining an Ammopiptanthus mongolicus (Maxim. ex kon) Chengf. explant; (2) carrying out Agrobacterium rhizogenes selection and activation; (3) carrying out secondary activation of Agrobacterium rhizogenes; (4) infecting the explant with the Agrobacterium rhizogenes; (5) carrying out hairy root induction and propagation; and (6) carrying out amplification culture. By means of the method, hairy roots capable of producing the formononetin can be obtained. The content of the formononetin produced by the hairy roots is remarkably increased and is increased by 14 times if being compared with that of wild plants. The Ammopiptanthus mongolicus (Maxim. ex kon) Chengf. hairy root has the advantages of high growth speed, short periods, high relative yield of secondary metabolites and the like, is not limited by seasons, and can be used for industrial production of the Ammopiptanthus mongolicus (Maxim. ex kon) Chengf. formononetin.