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Transporter protein for regulating and controlling salt resistance and drought resistance of plant and encoding gene and application thereof

A technology for encoding genes and anti-transporters, which is applied to the Na+/H+ anti-transporters of S. vulgaris and their encoded genes and application fields, and can solve problems such as staying in the physiological and biochemical stages.

Inactive Publication Date: 2012-09-05
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the stress tolerance of Ilex japonica only stays at the physiological and biochemical stage, and it is a blank to explain the stress resistance mechanism of Ilex japonica genetically.

Method used

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  • Transporter protein for regulating and controlling salt resistance and drought resistance of plant and encoding gene and application thereof
  • Transporter protein for regulating and controlling salt resistance and drought resistance of plant and encoding gene and application thereof
  • Transporter protein for regulating and controlling salt resistance and drought resistance of plant and encoding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1, holly holly Na + / H + Acquisition of genes encoding antiporters

[0023] 1.1 Salt stress treatment of Ilex japonica

[0024] The holly seeds were sterilized with 0.01% HgCl2 surface for 15 minutes, rinsed with sterile water for 5 times, soaked in sterile water at 37°C overnight and then transferred to 1 / 2 MS+1.5% sucrose+1.0% agar+200mmNaCl salt stress medium. Cultivate for one week at 24°C under 16h L / 8h D light.

[0025] 1.2 RNA extraction

[0026] Take about 0.1 g of the young root material of Ilex japonica treated with salt stress. After the liquid nitrogen is fully ground, transfer to a 1.5ml centrifuge tube, add 1ml (Invitrogen Company), after mixing, place at room temperature for 15 minutes, add 0.2ml chloroform:isoamyl alcohol (24:1), shake vigorously for 15 seconds, place at room temperature for 5 minutes, and centrifuge at 13000rpm at 4°C for 15 minutes. Take the supernatant and add an equal volume of isopropanol, mix carefully, place at r...

Embodiment 2

[0051] Example 2: Functional analysis of Na+ / H+ antiporter protein

[0052] 2.1 Sequence comparison analysis

[0053] The secondary structure of AmNHX1 and the homology with other species were analyzed by TMpred software and Genedoc software respectively. The analysis results showed that AmNHX1 has 12 transmembrane domains, such as Figure 5 shown; and has great homology with the Na+ / H+ antiporters of other species, and has the amiloride-binding sequence "FFIYLLPPI", which is highly conserved in other plants and mammalian NHE, Such as Figure 6 As shown, the blackened part in the figure is the consistent amino acid sequence, and the framed part is the amiloride binding sequence. The gene accession numbers of Arabidopsis thaliana, soybean, rice, barley and salt mustard are: NP_198067, AAY43006, NP_001060571, BAC56698, ABJ97691.

[0054] In order to analyze AmNHX1 with other species Na + / H + The phylogenetic relationship of antiporters, AmNHX1 and other species Na + / H ...

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Abstract

The invention belongs to the technical field of plant gene engineering, specifically discloses an Ammopiptanthus mongolicus Na+ / H+ inverse transporter protein and the encoding gene and application thereof. The Ammopiptanthus mongolicus Na+ / H+ inverse transporter protein provided by the invention is originated from Ammopiptanthus mongolicus and named as AmNHX1, and the amino acid sequence thereof is represented by SEQ ID NO: 2. The encoding gene of the Ammopiptanthus mongolicus Na+ / H+ inverse transporter protein is one of the following nucleotide sequences: 1) SEQ ID NO: 1 in the sequence table; and 2) polynucleotide for encoding the amino acid sequence represented by SEQ ID NO: 2 in the sequence table. The gene plays important roles in researching salt-resisting and drought-resisting molecular mechanism of Ammopiptanthus mongolicus and improving salt resistance and drought resistance of plant.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, in particular to a transport protein and its coding gene and application, in particular to the Na + / H + Antiporters and their coding genes and applications. Background technique [0002] Salt damage and drought are two limiting factors that limit the growth of crops. The area of ​​China's arid region accounts for 1 / 3 of the total area of ​​the country, and saline-alkali soil is widely distributed in it. The coexistence of drought and salinity seriously limits the increase of crop yield and agricultural development in our country. Therefore, it is of great practical and strategic significance to understand and study the mechanism of salt-alkali and drought-resistant plants; to clone salt- and drought-resistant genes from unique resource organisms in my country to obtain valuable gene resources; to cultivate drought- and saline-alkali-tolerant crops. [0003] During the long-t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N15/82C12N5/10A01H5/00
Inventor 蒯本科魏强郭玉娟孙镇菲梁宁菁
Owner FUDAN UNIV
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