This invention is fake unicell bacteria Na+ / H+ antiport albumen gene and its clone method, it relates to one kind of gene clone. One kind of fake unicell bacteria Na+ / H+ antiport albumen structure gene and one kind of quickly and economic clone method are provided, gene length is 1089bp, coding 362 amino acids. Procedures are that extra anti-salt fake unicell bacteria is inoculated and cultivated, whole DNA is extracted, primer is designed, PCR reaction and amplified product is gel imaged system sweep record, then the amplified about 1.1kb tripe is cut down. One gland piao lin deoxyribonucleotide (A) is added to end of PCR product, above clear liquid is abandoned, and 20muL weight distilled water is added to dissolve DNA. Carrier is connected with DNA to form single colony. White colony is selected and recombination particle is extracted by caustic cracking solution. Goal gene is identificated and anti-salt level of transformant is detected, clone gene of transformant is sequenced and weight plasmid of transformant is identificated, then colone gene is estimated.