31 results about "Antiporters" patented technology

The invention relates to a cadaverine-producing engineering strain, a construction method thereof and a method for producing cadaverine by using the strain. Based on a gene engineering technology, an L-lysine decarboxylase gene (cadA / LDC) and a cadaverine-lysine antiporter gene (cadB) are respectively or simultaneously cloned into Corynebacterium glutamicum and subjected to induced coexpression, the lysine is decarboxylated to generate cadaverine under the catalytic action of the CadA enzyme, and the cadaverine is transported out of cells by the CadB protein, thus constructing a high-yield cadaverine-producing engineering strain and establishing a novel one-step cadaverine synthesis method based on the fermentation of Corynebacterium glutamicum. The one-step cadaverine synthesis method based on microbial fermentation provides a new way for large-scale cadaverine production, thereby having great economic benefits and social benefits and wide market development prospects.

The present invention provides a novel Na+ / H+ antiporter protein expressed in the rape, coding sequence and its application for improving adverse resistance (salt-resistance and alkali-resistance) of plant. Said invention relates to the fusion gene construction body containing the described gene and new recombinant expresion vector with said construction body, also relates to the plant cell converted by said expression vector and the transgenic plant of the described gene produced by converted cell and its progeny, including plant seed and plant tissue. Said invention can make said gene be expressed in plant, and the obtained transgenic plant has enhanced adverse-resistance.

The present invention relates to the cloning, recombination and salt tolerance analysis of cotton Na+ / H+ reverse transport protein GhNHX1 gene, and belongs to the field of molecular biology and biological technology. Total RNA is extracted from cotton leaf treated with 0.4 mol / L NaCl solution and inversely transcribed into cDNA. Facultative primer is used for conventional PCR to obtain intermediate segment, and whole length cDNA is obtained through fast amplification in 3' and 5' ends. The gene is transformed to salt-sensitive yeast mutant to restore its salt tolerant capacity to some degree; and the gene is further transformed to tobacco to obtain transgenic plant capabl eof growing normally in salt concentration of 200 mmol / L. Therefore, the gene is one important salt tolerant gene and may be used in raising the salt tolerant capacity of plant for plantation in saline land.

The present invention discloses an E. coli engineering bacteria producing 1,5-pentanediamine through a whole cell catalysis and its application. The engineering bacteria according to the present invention, is Escherichia coli (E. coli) strain B or its derivative strains with the overexpression of a lysine decarboxylase gene and a proper expression of a lysine-cadaverine antiporter gene cadB. The engineering bacteria according to the present invention is the engineering bacteria producing 1,5-pentanediamine through the whole cell catalysis constructed from Escherichia coli B derivative strains, which has an overexpression of a lysine decarboxylase gene cadA and a proper expression of the lysine-cadaverine antiporter gene cadB. The present invention further discloses a method of producing a 1,5-pentanediamine catalyzed by the engineering bacteria, the yield and production intensity of 1,5-pentanediamine in bio-based production could be significantly improved through the method, hence it could be applied to mass production and convenient for extending applications.

The invention provides a novel CkNHX genen and excision adorn gene CkNHXn thereof. The gene stems from Caragana korshinskii Kom, its gene codes Na+ / H+ antiporter gene. The cDNA obtained by clone constructs yeast expression carrier and plant expression carrier. The yeast mutant strain vertifies salt resistance thereof by complament experiment. Moreover, a transgenic plant is also obtained. By testing salt resistance of transformed plant, transformation of the gene can make the transgenic plant with salt resistance.

The invention provides a novel plant strong salt tolerant gene AtNHXS1 acquired through DNA reshuffling technology, and the ionic transport activity of Na+ / H+ antiporter protein coded by the gene is stronger than that of wild Na+ / H+ antiporter protein ATNHX1. A nucleotide sequence of the gene as shown in SEQ ID NO:1 also comprises genes of the nucleotide sequence of sequence 1 in a sequence table with the homology between 70 and 100 percent, or a nucleotide sequence of an amino acid sequence of sequence 2 in a coded sequence table. The new Na+ / H+ antiporter has a protein of the amino acid sequence of sequence 2 in the sequence table, protein of the amino acid sequence of the sequence 2 with the homology between 70 and 100 percent, or protein which replaces, deletes or adds one or a plurality of amino acids in the amino acid sequence of the sequence 2 with the same homology. The invention also provides the construction of recombinant vector, the transgenic plant and other methods so as to apply the gene and the protein, thereby cultivating a novel variety of transgenic plant with strong capacity of salt tolerance or other improved biologic characters.

The invention relates to the field of plant genetic engineering and provides a novel plant strong salt-tolerant gene NHXS1 obtained by a restructuring technology. A nucleotide sequence of the plant strong salt-tolerant gene NHXS1 is SEQ ID NO: 1, or a DNA sequence which has 70 to 100 percent homology with the SEQ ID NO: 1 nucleotide sequence or a DNA sequence for coding a protein sequence of SEQ ID NO: 2. Na + / H + antiporter NHXS1 colded by the gene has stronger ion transport activity than the wild-type Na + / H + antiporter AtNHX1. The invention also provides methods for construction of recombinant vectors and transgenic plants to apply the genes and proteins, and can culture a novel breed of transgenic plant with stronger salt-tolerant property or other improved biological characteristics.

The present invention provides novel polynucleotides encoding plant Na+ / H+ antiporter polypeptides, fragments and homologs thereof. Also provided are vectors, host cells, antibodies, and recombinant methods for producing said polypeptides. The invention further relates to methods of applying these novel plants polypeptides to the identification, prevention, and / or conferment of resistence to various plant diseases and / or disorders, particularly those associated with modulating environmental stress responses, such as drought and salt tolerance.

The invention discloses a gene sequence of Aneurolepidiumchinense Na+ / H+ antiport protein, a clone and application thereof, belonging to the molecular biology field. A coded gene of vacuole type Na+ / H+ antiport protein is separated from the Aneurolepidiumchinense for the first time with RT-PCR through designing a primer according to an amino acid sequence of the Na+ / H+ antipor protein published in other plants. With the transfer of the gene, the anti-salt property of a salt-sensitive yeast mutant can be recovered to a certain degree. A transgenic plant has higher salt tolerance which can reach 200mM through further constructing a sense expression vector and transforming tobacco. The gene can be used in genetic transformation of a plant to improve the salt tolerance of the plant.

The invention relates to the cloning, recombination, salt tolerance function analysis and application of alfalfa Na+ / H+ antiporter MsNHX1 gene, and belongs to the field of molecular biology and biotechnology. Total RNA was extracted from alfalfa roots, and then 1 μg of total RNA was reverse transcribed into cDNA. According to the conserved amino acid sequences of Na+ / H+ antiporters in other plants, a pair of degenerate primers were designed, and conventional polymerase chain reaction (PCR) was performed. The PCR product was connected to the pGEM-T vector and transformed into DH5α cells. Perform sequence determination. The full-length cDNA was then obtained by rapid amplification of the 3' and 5' ends. Further construct the sense expression vector and transform Arabidopsis. Transgenic Arabidopsis has higher salt tolerance. Therefore, the gene transformation of dicotyledonous crops such as alfalfa will improve their salt tolerance, yield and quality, thereby expanding the planting area of ​​dicotyledonous plants on saline-alkali land and generating huge economic and social benefits.

An SOS gene has been isolated through PCR cloning from Thellungiella halophila and is designated TSOS1. The polynucleotide encodes a transmembrane protein with similarities to plasma membrane Na+ / H+ antiporters from bacteria and fungi. The invention encompasses the TSOS1 gene, the corresponding protein, closely related polypeptides that confer salt tolerance, and related polynucleotides that encode a polypeptide that has Na+ / H+ antiporter activity.

The present invention provides isolated or genetically modified strains of microorganisms that display enhanced resistance to acetate as a result of increased expression of a sodium proton antiporter. The present invention also provides methods for producing such microbial strains, as well as related promoter sequences and expression vectors. Further, the present invention provides methods of producing alcohol from biomass materials by using microorganisms with enhanced resistance to acetate.

Compositions and methods useful in identifying and counter-selecting maize plants with having enhanced yield-related traits relative to control plants under abiotic stress conditions such as salt stress and / or drought are provided herein. The methods use molecular genetic markers to identify, select and / or construct salt stress tolerant and / or drought tolerant maize plants. Also provided are methods to enhance tolerance to salt stress and / or drought in crop plants by transforming crop plants with the Zea mays antiporter / sodiunn ion transporter or by introducing favorable allelic variants of the Zea mays antiporter / sodiunn ion transporter gene via gene editing.

The present invention belongs to the cloning, recombination and salt endurance function analysis of couchgrass sodium ion reverse transport protein ENHX1 gene, and belongs to the field of molecular biology and biological technology. General RNA is first extracted from couchgrass root and then inverse transcripted into cDNA. A pair of facultative primer is designed based on the conservative amino acid sequence of other plant's sodium ion reverse transport protein CycD2 for conventional PCR, land the PCR product is connected to pGEM-The vector to convert DH5 alpha cell and determine sequence. Then, whole length cDNA is obtained via fast amplification in 3' and 5' ends. Further, positive-sense expression vector is constituted to convert pseudomustard.

The invention provides a novel CkNHX genen and excision adorn gene CkNHXn thereof. The gene stems from Caragana korshinskii Kom, its gene codes Na+ / H+ antiporter gene. The cDNA obtained by clone constructs yeast expression carrier and plant expression carrier. The yeast mutant strain vertifies salt resistance thereof by complament experiment. Moreover, a transgenic plant is also obtained. By testing salt resistance of transformed plant, transformation of the gene can make the transgenic plant with salt resistance.

The present invention provides isolated or genetically modified strains of microorganisms that display enhanced resistance to acetate as a result of increased expression of a sodium proton antiporter. The present invention also provides methods for producing such microbial strains, as well as related promoter sequences and expression vectors. Further, the present invention provides methods of producing alcohol from biomass materials by using microorganisms with enhanced resistance to acetate.

The invention provides cDNA of a switchgrass tonoplast Na<+> / H<+> antiporter protein gene PvNHX1, and is characterized in that the cDNA has a nucleotide sequence shown in SEQ ID No.1. The invention also provides a cloning method of the cDNA of the switchgrass tonoplast Na<+> / H<+> antiporter protein gene PvNHX1 according to the claim 1, wherein the method comprises the specific steps: (1) extracting total RNA of switchgrass, and carrying out reverse transcription into cDNA; (2) taking the cDNA as a template, and carrying out a high-fidelity full-length PCR reaction by a PvNHX1 gene full-length specific primer; and (3) recovering and purifying the PCR product of the step (2), carrying out an A addition reaction, and thus obtaining the cDNA of the switchgrass tonoplast Na<+> / H<+> antiporter protein gene PvNHX1. The method for obtaining the switchgrass PvNHX1 gene full-length cDNA sequence lays a foundation for vector construction, genetic transformation and the like by utilizing the sequence later on.

An SOS gene has been isolated through PCR cloning from Thellungiella halophila and is designated TSOS1. The polynucleotide encodes a transmembrane protein with similarities to plasma membrane Na+ / H+ antiporters from bacteria and fungi. The invention encompasses the TSOS1 gene, the corresponding protein, closely related polypeptides that confer salt tolerance, and related polynucleotides that encode a polypeptide that has Na+ / H+ antiporter activity.

The present invention discloses a Na / H antiporter gene ZjNHX1 coming from zoysia japonica, an encoding protein and an application thereof. The gene is one of the following nucleotide sequences: (1) SEQ ID No:1 in the sequence table; (2) SEQ ID No:2 protein in the coding sequence table, and a nucleotide sequence 70 percent homologous to the 409th position to 2031th position of SEQ ID No:1 of the sequence table; (3) a nucleotide sequence capable of being hybridized with the 409th position to 2031th position of SEQ ID No:1 sequence in the sequence table under the medium stringency condition. The result of a functional complementation experiment conducted between the ZjNHX1 and the single and the double mutant of salt-sensitive yeast shows that the gene can reinstate the anti-salt capability of the salt-sensitive yeast to a certain degree. The present invention is applicable to the cultivation of novel salt-tolerant plant varieties or other novel genetically modified plant varieties with improved biological characters.

A Na<+> / H+ antiporter gene on a wheat plasma membrane and applications thereof are disclosed. The full length of the antiporter gene is 3460 bp. The gene after expression is positioned on a cell plasma membrane, and exerts a Na<+> / H+ exchange function to discharge Na<+> out of cells. A method of constructing a plant genetic transformation vector by utilizing the antiporter gene and improving plant salt tolerance is also disclosed.

The invention provides a novel plant strong salt tolerant gene AtNHXS1 acquired through DNA reshuffling technology, and the ionic transport activity of Na+ / H+ antiporter protein coded by the gene is stronger than that of wild Na+ / H+ antiporter protein ATNHX1. A nucleotide sequence of the gene as shown in SEQ ID NO:1 also comprises genes of the nucleotide sequence of sequence 1 in a sequence tablewith the homology between 70 and 100 percent, or a nucleotide sequence of an amino acid sequence of sequence 2 in a coded sequence table. The new Na+ / H+ antiporter has a protein of the amino acid sequence of sequence 2 in the sequence table, protein of the amino acid sequence of the sequence 2 with the homology between 70 and 100 percent, or protein which replaces, deletes or adds one or a plurality of amino acids in the amino acid sequence of the sequence 2 with the same homology. The invention also provides the construction of recombinant vector, the transgenic plant and other methods so asto apply the gene and the protein, thereby cultivating a novel variety of transgenic plant with strong capacity of salt tolerance or other improved biologic characters.

The present invention relates to the cloning, recombination and salt tolerance analysis of cotton Na+ / H+ reverse transport protein GhNHX1 gene, and belongs to the field of molecular biology and biological technology. Total RNA is extracted from cotton leaf treated with 0.4 mol / L NaCl solution and inversely transcribed into cDNA. Facultative primer is used for conventional PCR to obtain intermediate segment, and whole length cDNA is obtained through fast amplification in 3' and 5' ends. The gene is transformed to salt-sensitive yeast mutant to restore its salt tolerant capacity to some degree; and the gene is further transformed to tobacco to obtain transgenic plant capabl eof growing normally in salt concentration of 200 mmol / L. Therefore, the gene is one important salt tolerant gene and may be used in raising the salt tolerant capacity of plant for plantation in saline land.

The invention relates to the field of plant gene engineering, providing a buckwheat Na+ / H+ antiporter FtNHX. The buckwheat Na+ / H+ antiporter FtNHX is provided with SEQ ID No:2 amino acid residue sequence in a sequence table, or the buckwheat Na+ / H+ antiporter FtNHX is obtained by the substitution, deletion or adding of one or multiple amino acid residues by SEQ ID No: 2 in the sequence table, has the same activity with the SEQ ID No:2 amino acid residue sequence and is derived by SEQ ID No:2. The gene for coding the protein is preferably selected from a nucleotide sequence comprising the 288th-1949th bit of SEQ ID No:1, or the gene is a nucleotide sequence which has more than 90% of homology with a DNA sequence specified by the SEQ ID No:1 and codes same functional protein. The invention also provides methods for recombinant vector construction, transgenic plant and the like to apply the above gene and protein, and can cultivate new improved gene plant species with stronger salt tolerance or other biological characters.

Imaging agents are described that comprise labeled substrates capable of being introduced into cells via the cystine / glutamate antiporter. The substrates may be used for imaging or detecting oxidative stress in cells by introducing the labeled agents into cells via the cystine / glutamate antiporter and subsequent detection.

A method for cultivating sugar beet with strong salt tolerance and drought resistance, the purpose of which is to avoid the defects of traditional breeding and the lack of previous techniques for single gene transformation, and realize the combination of two Na+ / H+ antiporter genes ZxNHX and vacuolar Membrane H+-pyrophosphatase gene ZxVP1-1 is polymerized, and a new transgenic sugar beet variety (line) co-expressing ZxNHX and ZxVP1-1 is obtained through genetic transformation, providing a method for cultivating sugar beets with strong salt tolerance and drought resistance and high sugar content; The steps include seedling cultivation, seedling infection, molecular identification and stress resistance evaluation. This method greatly shortens the cultivation time of transgenic seedlings, the whole cycle is controlled at about 5 months, no callus is required, and the genetic transformation efficiency is as high as 10%.

Provided herein are compositions and methods useful for identifying and counter-selecting maize plants having enhanced yield-related traits relative to control plants under abiotic stress conditions, such as salt stress and / or drought. The methods use molecular genetic markers to identify, select and / or construct salt stress tolerant and / or drought tolerant maize plants. Also provided is the enhancement of crop plants to salt stress and / or by transforming crop plants with maize antiporter / sodium ion transporter or by introducing favorable allelic variants of maize antiporter / sodium ion transporter gene via gene editing or drought tolerance methods.

The invention relates to a K<+> / H<+>antiporter protein. The K<+> / H<+>antiporter protein has an amino acid sequence represented by SEQ ID No:2, or has an amino acid sequence obtained through substituting, deleting or adding one or more amino acid residues to the amino acid sequence represented by the SEQ ID No:2 and still having K<+> / H<+>antiporter protein activities. The invention also relates to a coding gene of the K<+> / H<+>antiporter protein. The gene has a nucleotide sequence represented by SEQ ID No:1, or has a nucleotide sequence coding the amino acid sequence represented by SEQ ID No:2. The invention further relates to a recombinant vector containing the K<+> / H<+>antiporter gene, a cell containing the K<+> / H<+>antiporter gene, and applications of the K<+> / H<+>antiporter protein, the coding gene thereof, the recombinant vector containing the gene and the cell containing the gene in the cultivation of alkali-resistant transgenic organisms.

This invention is fake unicell bacteria Na+ / H+ antiport albumen gene and its clone method, it relates to one kind of gene clone. One kind of fake unicell bacteria Na+ / H+ antiport albumen structure gene and one kind of quickly and economic clone method are provided, gene length is 1089bp, coding 362 amino acids. Procedures are that extra anti-salt fake unicell bacteria is inoculated and cultivated, whole DNA is extracted, primer is designed, PCR reaction and amplified product is gel imaged system sweep record, then the amplified about 1.1kb tripe is cut down. One gland piao lin deoxyribonucleotide (A) is added to end of PCR product, above clear liquid is abandoned, and 20muL weight distilled water is added to dissolve DNA. Carrier is connected with DNA to form single colony. White colony is selected and recombination particle is extracted by caustic cracking solution. Goal gene is identificated and anti-salt level of transformant is detected, clone gene of transformant is sequenced and weight plasmid of transformant is identificated, then colone gene is estimated.

The present invention relates to cloning, recombination, salt endurance function analysis and applications of Na+ / H+ reverse transport protein MsNHX genes in alfalfa, and belongs to molecular biology and biological technology field. The total RNA is extracted from roots of alfalfa, then 1 microgramme total RNA is reversely transcripted into cDNA. According to conservative amino acid sequences in Na+ / H+ reverse transport protein in other plants, a pair of degenerated primers are designed to execute a routine polyase chain reaction (PCR), wherein the PCR product is connected to a pGEM-T carrier to converse DH5 alpha cells and execute sequence determination. Then, 3' and 5' tail ends rapid amplifications are executed to obtain cDNA with full-length. Right expression carriers are further constructed for conversion of arabidopsis thaliana. The transgenic arabidopsis thaliana possesses higher salt resistant capability. Hence the transgenic double-seminal leaf crops such as heart trefoil will improve its salt resistant capability, yield and quality, then the planted area of dicotyledon at alkaline land can be expanded to generate huge economic benefit and social benefit.